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Protein

Malate dehydrogenase [NADP], chloroplastic

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activityi

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulationi

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei76Activation of NADP-MDH1
Sitei81Activation of NADP-MDH1
Binding sitei186SubstratePROSITE-ProRule annotation1
Binding sitei192SubstratePROSITE-ProRule annotation1
Binding sitei199NADPBy similarity1
Binding sitei206NADBy similarity1
Binding sitei225SubstratePROSITE-ProRule annotation1
Binding sitei256SubstratePROSITE-ProRule annotation1
Active sitei281Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi105 – 111NADPBy similarity7
Nucleotide bindingi223 – 225NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

SABIO-RKP21528.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase [NADP], chloroplastic (EC:1.1.1.82)
Alternative name(s):
NADP-MDH
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 58Chloroplast1 PublicationAdd BLAST58
ChainiPRO_000001864659 – 441Malate dehydrogenase [NADP], chloroplasticAdd BLAST383

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi76 ↔ 81In oxidized inactive NAD-MDH1 Publication
Disulfide bondi417 ↔ 429In oxidized inactive NAD-MDHBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP21528.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP21528. 1 interactor.
MINTiMINT-2584130.

Structurei

3D structure databases

ProteinModelPortaliP21528.
SMRiP21528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTQLNSTC SKPQLHSSSQ LSFLSRTRTR TLPRHYHSTF APLHRTQHAR
60 70 80 90 100
ISCSVAPNQV QVPAAQTQDP KGKPDCYGVF CLTYDLKAEE ETKSWKKLIN
110 120 130 140 150
IAVSGAAGMI SNHLLFKLAS GEVFGPDQPI ALKLLGSERS IQALEGVAME
160 170 180 190 200
LEDSLFPLLR EVVISIDPYE VFQDAEWALL IGAKPRGPGV ERAALLDING
210 220 230 240 250
QIFAEQGKAL NAVASRNAKV IVVGNPCNTN ALICLKNAPN IPAKNFHALT
260 270 280 290 300
RLDENRAKCQ LALKAGVFYD KVSNMTIWGN HSTTQVPDFL NARIDGLPVK
310 320 330 340 350
EVIKDNKWLE EEFTEKVQKR GGVLIQKWGR SSAASTSVSI VDAIRSLITP
360 370 380 390 400
TPEGDWFSSG VYTNGNPYGI AEDIVFSMPC RSKGDGDYEL VNDVIFDDYL
410 420 430 440
RQKLAKTEAE LLAEKKCVAH LTGEGIAVCD LPGDTMLPGE M
Length:441
Mass (Da):48,264
Last modified:February 1, 1994 - v2
Checksum:i613747E6357C325B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90E → T AA sequence (PubMed:1986782).Curated1
Sequence conflicti234C → M AA sequence (PubMed:3665938).Curated1
Sequence conflicti287P → K AA sequence (PubMed:3665938).Curated1
Sequence conflicti294I → L AA sequence (PubMed:1986782).Curated1
Sequence conflicti297L → I AA sequence (PubMed:1986782).Curated1
Sequence conflicti366N → V AA sequence (PubMed:1986782).Curated1
Sequence conflicti377S → G AA sequence (PubMed:1986782).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74507 mRNA. Translation: CAA52614.1.
PIRiS38346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74507 mRNA. Translation: CAA52614.1.
PIRiS38346.

3D structure databases

ProteinModelPortaliP21528.
SMRiP21528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP21528. 1 interactor.
MINTiMINT-2584130.

Proteomic databases

PRIDEiP21528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP21528.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDHP_PEA
AccessioniPrimary (citable) accession number: P21528
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.