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P21528

- MDHP_PEA

UniProt

P21528 - MDHP_PEA

Protein

Malate dehydrogenase [NADP], chloroplastic

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

    Catalytic activityi

    (S)-malate + NADP+ = oxaloacetate + NADPH.

    Enzyme regulationi

    Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei76 – 761Activation of NADP-MDH
    Sitei81 – 811Activation of NADP-MDH
    Binding sitei186 – 1861SubstratePROSITE-ProRule annotation
    Binding sitei192 – 1921SubstratePROSITE-ProRule annotation
    Binding sitei199 – 1991NADPBy similarity
    Binding sitei206 – 2061NADBy similarity
    Binding sitei225 – 2251SubstratePROSITE-ProRule annotation
    Binding sitei256 – 2561SubstratePROSITE-ProRule annotation
    Active sitei281 – 2811Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi105 – 1117NADPBy similarity
    Nucleotide bindingi223 – 2253NADPBy similarity

    GO - Molecular functioni

    1. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase [NADP], chloroplastic (EC:1.1.1.82)
    Alternative name(s):
    NADP-MDH
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5858Chloroplast1 PublicationAdd
    BLAST
    Chaini59 – 441383Malate dehydrogenase [NADP], chloroplasticPRO_0000018646Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi76 ↔ 81In oxidized inactive NAD-MDH1 Publication
    Disulfide bondi417 ↔ 429In oxidized inactive NAD-MDHBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP21528.
    ProMEXiP21528.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiP21528. 1 interaction.
    MINTiMINT-2584130.

    Structurei

    3D structure databases

    ProteinModelPortaliP21528.
    SMRiP21528. Positions 71-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011273. Malate_DH_NADP-dep_pln.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
    TIGR01759. MalateDH-SF1. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21528-1 [UniParc]FASTAAdd to Basket

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    MALTQLNSTC SKPQLHSSSQ LSFLSRTRTR TLPRHYHSTF APLHRTQHAR    50
    ISCSVAPNQV QVPAAQTQDP KGKPDCYGVF CLTYDLKAEE ETKSWKKLIN 100
    IAVSGAAGMI SNHLLFKLAS GEVFGPDQPI ALKLLGSERS IQALEGVAME 150
    LEDSLFPLLR EVVISIDPYE VFQDAEWALL IGAKPRGPGV ERAALLDING 200
    QIFAEQGKAL NAVASRNAKV IVVGNPCNTN ALICLKNAPN IPAKNFHALT 250
    RLDENRAKCQ LALKAGVFYD KVSNMTIWGN HSTTQVPDFL NARIDGLPVK 300
    EVIKDNKWLE EEFTEKVQKR GGVLIQKWGR SSAASTSVSI VDAIRSLITP 350
    TPEGDWFSSG VYTNGNPYGI AEDIVFSMPC RSKGDGDYEL VNDVIFDDYL 400
    RQKLAKTEAE LLAEKKCVAH LTGEGIAVCD LPGDTMLPGE M 441
    Length:441
    Mass (Da):48,264
    Last modified:February 1, 1994 - v2
    Checksum:i613747E6357C325B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti90 – 901E → T AA sequence (PubMed:1986782)Curated
    Sequence conflicti234 – 2341C → M AA sequence (PubMed:3665938)Curated
    Sequence conflicti287 – 2871P → K AA sequence (PubMed:3665938)Curated
    Sequence conflicti294 – 2941I → L AA sequence (PubMed:1986782)Curated
    Sequence conflicti297 – 2971L → I AA sequence (PubMed:1986782)Curated
    Sequence conflicti366 – 3661N → V AA sequence (PubMed:1986782)Curated
    Sequence conflicti377 – 3771S → G AA sequence (PubMed:1986782)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74507 mRNA. Translation: CAA52614.1.
    PIRiS38346.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74507 mRNA. Translation: CAA52614.1 .
    PIRi S38346.

    3D structure databases

    ProteinModelPortali P21528.
    SMRi P21528. Positions 71-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P21528. 1 interaction.
    MINTi MINT-2584130.

    Proteomic databases

    PRIDEi P21528.
    ProMEXi P21528.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011273. Malate_DH_NADP-dep_pln.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01757. Malate-DH_plant. 1 hit.
    TIGR01759. MalateDH-SF1. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, site-specific mutagenesis, expression and characterization of full-length chloroplast NADP-malate dehydrogenase from Pisum sativum."
      Reng W., Riessland R., Scheibe R., Jaenicke R.
      Eur. J. Biochem. 217:189-197(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Primary structure and analysis of the location of the regulatory disulfide bond of pea chloroplast NADP-malate dehydrogenase."
      Scheibe R., Kampfenkel K., Wessels R., Tripier D.
      Biochim. Biophys. Acta 1076:1-8(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 65-441, DISULFIDE BOND.
      Strain: cv. Kleine Rheinlaenderin.
    3. "Amino acid sequence similarity between malate dehydrogenases (NAD) and pea chloroplast malate dehydrogenase (NADP)."
      Fickenscher K., Scheibe R., Marcus F.
      Eur. J. Biochem. 168:653-658(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
    4. "Limited proteolysis of NADP-malate dehydrogenase from pea chloroplast by aminopeptidase K yields monomers. Evidence of proteolytic degradation of NADP-malate dehydrogenase during purification from pea."
      Kampfenkel K.
      Biochim. Biophys. Acta 1156:71-77(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 59-74 AND 92-112, SUBUNIT.
      Strain: cv. Kleine Rheinlaenderin.

    Entry informationi

    Entry nameiMDHP_PEA
    AccessioniPrimary (citable) accession number: P21528
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3