P21526 (HN_PI4HA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hemagglutinin-neuraminidase
EC=3.2.1.18

Gene names

Name:

HN

Organism

Human parainfluenza 4a virus (strain Toshiba) (HPIV-4a)

Taxonomic identifier

11225 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus › Human parainfluenza virus 4 › Human parainfluenza virus 4a

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Host cell membrane Host membrane Membrane Viral envelope protein Virion
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hemagglutinin Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Hemagglutinin-neuraminidase		PRO_0000142632
Regions
	Topological domain	1 – 27	27	Intravirion Potential
	Transmembrane	28 – 48	21	Helical; Potential
	Topological domain	49 – 573	525	Virion surface Potential
Amino acid modifications
	Glycosylation	258	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	330	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	339	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	347	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	433	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	502	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	530	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P21526 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 3540F24F17931AC4	FASTA	573	65,544
	10 20 30 40 50 60 MQDSHGNTQI LNQANSMVKR TWRLLFRIAT LILLVSIFVL SLIIVLQSTP GNLQNDINII 70 80 90 100 110 120 RKELNELMEN FETTSKSLLS VSNQITYDVS VLTPIRQEAI ETNIISKIKD HCKDRVIKEG 130 140 150 160 170 180 STCTLNRSPL HDVSFLNGFN KFYFTYKDNM QIKFKSLLDY PNFIPTATTP HGCIRIPSFS 190 200 210 220 230 240 LGQTHWCYTH NINLLGCADP ASSNQYVSLG TLQVLKMGDP YFKVEHSHYL NDGRNRKSCS 250 260 270 280 290 300 VVAVPDGCLR NCVTMTKNET ENFKDLNWQH NYLHTYHIMV PLKTRIINPP GSSRDWVHIA 310 320 330 340 350 360 PGVGSGLLYA KLLIFPLYGG LTEKSVIHNN QSGKYFFPNS TKLQCRNSTM EKIKGAKDSY 370 380 390 400 410 420 TITYFSGRLI QSAFLVCDLR QFLSEDCEIL IPSNDYMMVG AEGRLYNIEN NIFYYQRGSS 430 440 450 460 470 480 WWPYPSLYRI RLNLSKKYPR ITEIKFTKIE IAPRPGNKDC PGNKACPKEC ITGVYQDILP 490 500 510 520 530 540 LSYPNTAFPH LKQAYYTGFY LNNSLERRNP TFYTADNLDY HQQERLGKFN LTAGYSTTTC 550 560 570 FKQTTTARLY CLYIIEVGDS VIGDFQITLF LAA

References

[1]

"Molecular cloning and sequence analysis of human parainfluenza type 4A virus HN gene: its irregularities on structure and activities." Bando H., Kondo K., Kawano M., Komada H., Tsurudome M., Nishio M., Ito Y. Virology 175:307-312(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34033 Genomic RNA. Translation: AAA46799.1.
PIR	HNNZ4A. A34683.
3D structure databases
ProteinModelPortal	P21526.
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.120.10.10. 1 hit.
InterPro	IPR000665. Hemagglutn-neuramid. IPR016285. Hemagglutn-neuramid_paramyxo. IPR011040. Sialidases. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAM	SSF50939. Sialidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_PI4HA

Accession

Primary (citable) accession number: P21526

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program