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Protein

Transcription factor kayak, isoforms A/B/F

Gene

kay

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Developmentally regulated transcription factor AP-1 binds and recognizes the enhancer DNA sequence: 5'-TGA[CG]TCA-3'. May play a role in the function or determination of a particular subset of cells in the developing embryo. It is able to carry out its function either independently of or in conjunction with Jra.1 Publication

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • protein heterodimerization activity Source: FlyBase
  • repressing transcription factor binding Source: FlyBase
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: GO_Central
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: FlyBase
  • transcription factor activity, sequence-specific DNA binding Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • adult chitin-containing cuticle pigmentation Source: FlyBase
  • antimicrobial humoral response Source: FlyBase
  • border follicle cell migration Source: FlyBase
  • collateral sprouting of injured axon Source: FlyBase
  • compound eye development Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • dorsal closure Source: FlyBase
  • embryo development Source: UniProtKB
  • establishment of planar polarity Source: FlyBase
  • germ cell development Source: FlyBase
  • imaginal disc fusion, thorax closure Source: FlyBase
  • JNK cascade Source: FlyBase
  • locomotor rhythm Source: FlyBase
  • ovarian follicle cell migration Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • R3/R4 cell fate commitment Source: FlyBase
  • regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle Source: FlyBase
  • response to wounding Source: FlyBase
  • second mitotic wave involved in compound eye morphogenesis Source: FlyBase
  • synaptic growth at neuromuscular junction Source: FlyBase
  • wing disc development Source: FlyBase
  • wound healing Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-DME-2559580. Oxidative Stress Induced Senescence.
R-DME-2871796. FCERI mediated MAPK activation.
R-DME-450341. Activation of the AP-1 family of transcription factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor kayak, isoforms A/B/F
Alternative name(s):
AP-1
Fos-related antigen
Short name:
Dfos
Short name:
dFra
Gene namesi
Name:kay
Synonyms:Fra
ORF Names:CG15509
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0001297. kay.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • nucleus Source: FlyBase
  • transcription factor complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Loss of isoform A causes embryonic lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764781 – 755Transcription factor kayak, isoforms A/B/FAdd BLAST755

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei548Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21525.
PRIDEiP21525.

PTM databases

iPTMnetiP21525.

Expressioni

Tissue specificityi

Early expression in the embryo is mesodermal and some of this expression is localized to a region surrounding the cephalic furrow. Later in embryonic development expression is ectodermal, corresponding to muscle attachment sites. Also observed in part of the mid- and hindgut and in the anal pad.1 Publication

Developmental stagei

Isoform A and isoform B are expressed both maternally and zygotically. Zygotically expressed throughout embryogenesis, until second larval instar. Isoform A is more highly expressed than isoform B.2 Publications

Gene expression databases

BgeeiFBgn0001297.
ExpressionAtlasiP21525. baseline.
GenevisibleiP21525. DM.

Interactioni

Subunit structurei

Homodimer. Heterodimer with Jra. The kay-Jra heterodimer binds more stably to the AP-1 site than either of the two proteins alone.

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • repressing transcription factor binding Source: FlyBase
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi533679. 18 interactors.
DIPiDIP-22245N.
IntActiP21525. 2 interactors.
MINTiMINT-855437.
STRINGi7227.FBpp0288515.

Structurei

3D structure databases

ProteinModelPortaliP21525.
SMRiP21525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini418 – 481bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni420 – 439Basic motifPROSITE-ProRule annotationAdd BLAST20
Regioni446 – 453Leucine-zipperPROSITE-ProRule annotation8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi19 – 150Gln-richAdd BLAST132
Compositional biasi176 – 273Thr-richAdd BLAST98

Sequence similaritiesi

Belongs to the bZIP family. Fos subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1414. Eukaryota.
ENOG4111CH5. LUCA.
GeneTreeiENSGT00730000110541.
InParanoidiP21525.
KOiK09031.
OMAiYQNGHIM.
OrthoDBiEOG091G0FN2.
PhylomeDBiP21525.

Family and domain databases

InterProiIPR000837. AP-1.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR23351. PTHR23351. 1 hit.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
PRINTSiPR00042. LEUZIPPRFOS.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform F (identifier: P21525-5) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKNLNGRTHN ACYHPYYHQS LHFAQQQQQQ QQHHLQQQQQ HMQQQQQQQQ
60 70 80 90 100
APQQQLRHQQ RQLPTQPAYQ QSQSVAHNAF PLRSSSNNYG HVASSAYAAS
110 120 130 140 150
SGSHNSNNAA AMAAVCQMQN FFNQQQQQQQ QLEFNNNCMP INYYQQQQQQ
160 170 180 190 200
HYPSESQSSA SGWNPETPGQ AQLALTATTC NTTAAATCNT TAAATTSTTA
210 220 230 240 250
TSAAAGSDNN HSDNFAMDAS EIATFLANEL FLQQLGNFET GQSVLTLTTP
260 270 280 290 300
TLTPTTTRNI EDTLGHLLSD TQTDRVAGCA GFAVPKVLPN AIDVLGMGIP
310 320 330 340 350
TGVSSLPLQQ TFDLSLGQGS ESEDSNASYN DTQMNEEQDT TDTSSAHTDS
360 370 380 390 400
TSYQAGHIMA GSVNGGGVNN FSNVLAAVSS SRGSASVGSS NANTSNTPAR
410 420 430 440 450
RGGGRRPNRS TNMTPEEEQK RAVRRERNKQ AAARCRKRRV DQTNELTEEV
460 470 480 490 500
EQLEKRGESM RKEIEVLTNS KNQLEYLLAT HRATCQKIRS DMLSVVTCNG
510 520 530 540 550
LIAPAGLLSA GSSGSGASSH HNHNSNDSSN GTITGMDATL NSTGRSNSPL
560 570 580 590 600
DLKPAANIDS LLMHIKDEPL DGAIDSGSSL DQDGPPPSKR ITLPPMSTMP
610 620 630 640 650
HVHLSTILTP TGASSGSLQT PITSTAPGGF GSAFPVTSNG SSINNINSIG
660 670 680 690 700
NNMNSPTLNA HNKVPKERPN TLAFQRPLGQ MHLTMANNKA GGPTQIQGVP
710 720 730 740 750
IQTPSTGTFN FDSLMDGGTG LTPVSGPLVP NSSSTNKHPL ELPTPTAEPS

KLVSL
Note: No experimental confirmation available.
Length:755
Mass (Da):80,579
Last modified:June 16, 2009 - v4
Checksum:i6E21F0460B626DAA
GO
Isoform A (identifier: P21525-2) [UniParc]FASTAAdd to basket
Also known as: beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: MKNLNGRTHN...FLANELFLQQ → MKVKVERTTK...KIATPTRNLI

Note: Produced by alternative promoter usage.Curated
Show »
Length:595
Mass (Da):62,812
Checksum:i99B46F99444141A7
GO
Isoform B (identifier: P21525-3) [UniParc]FASTAAdd to basket
Also known as: gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: MKNLNGRTHN...FLANELFLQQ → MTLDSYNIFNDEYLFNMPLSPLPKV

Note: Produced by alternative promoter usage.
Show »
Length:546
Mass (Da):57,483
Checksum:iAAC27E0A7AC6F27F
GO
Isoform D (identifier: A8MPH9-1) [UniParc]FASTAAdd to basket
Also known as: alpha
The sequence of this isoform can be found in the external entry A8MPH9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative promoter usage.
Length:722
Mass (Da):78,320
GO
Isoform sro (identifier: A8MPH9-2) [UniParc]FASTAAdd to basket
Also known as: shroud
The sequence of this isoform can be found in the external entry A8MPH9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: May perform a regulatory function, acting as a dominant negative regulator of isoform alpha.
Length:210
Mass (Da):24,742
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti613A → P in CAA38082 (PubMed:2116361).Curated1
Isoform A (identifier: P21525-2)
Sequence conflicti19D → N in AAK11267 (PubMed:11470538).Curated1
Sequence conflicti19D → N in AAK11268 (PubMed:11470538).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0178911 – 234MKNLN…LFLQQ → MKVKVERTTKKPAIRKPEDP DPAEEDRVKMVQDDPEDQEN QAVDEEELDFLPADLSAAIS TATTKIATPTRNLI in isoform A. 3 PublicationsAdd BLAST234
Alternative sequenceiVSP_0178921 – 234MKNLN…LFLQQ → MTLDSYNIFNDEYLFNMPLS PLPKV in isoform B. 2 PublicationsAdd BLAST234

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54143 mRNA. Translation: CAA38082.1.
AF238310 mRNA. Translation: AAF69496.1.
AY574373 mRNA. Translation: AAS87366.1.
AH010388 mRNA. Translation: AAK11268.2.
AF332660 mRNA. Translation: AAK11267.2.
DQ858476 mRNA. Translation: ABI74758.1.
AE014297 Genomic DNA. Translation: AAZ83992.1.
AE014297 Genomic DNA. Translation: AAZ83993.1.
AE014297 Genomic DNA. Translation: AAZ83994.2.
AY069805 mRNA. Translation: AAL39950.1.
PIRiA35847.
RefSeqiNP_001027577.2. NM_001032405.4. [P21525-5]
NP_001027578.1. NM_001032406.2. [P21525-3]
NP_001027579.1. NM_001032407.3. [P21525-2]
UniGeneiDm.6968.

Genome annotation databases

EnsemblMetazoaiFBtr0290076; FBpp0288515; FBgn0001297. [P21525-5]
GeneIDi3772082.
KEGGidme:Dmel_CG33956.

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54143 mRNA. Translation: CAA38082.1.
AF238310 mRNA. Translation: AAF69496.1.
AY574373 mRNA. Translation: AAS87366.1.
AH010388 mRNA. Translation: AAK11268.2.
AF332660 mRNA. Translation: AAK11267.2.
DQ858476 mRNA. Translation: ABI74758.1.
AE014297 Genomic DNA. Translation: AAZ83992.1.
AE014297 Genomic DNA. Translation: AAZ83993.1.
AE014297 Genomic DNA. Translation: AAZ83994.2.
AY069805 mRNA. Translation: AAL39950.1.
PIRiA35847.
RefSeqiNP_001027577.2. NM_001032405.4. [P21525-5]
NP_001027578.1. NM_001032406.2. [P21525-3]
NP_001027579.1. NM_001032407.3. [P21525-2]
UniGeneiDm.6968.

3D structure databases

ProteinModelPortaliP21525.
SMRiP21525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi533679. 18 interactors.
DIPiDIP-22245N.
IntActiP21525. 2 interactors.
MINTiMINT-855437.
STRINGi7227.FBpp0288515.

PTM databases

iPTMnetiP21525.

Proteomic databases

PaxDbiP21525.
PRIDEiP21525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0290076; FBpp0288515; FBgn0001297. [P21525-5]
GeneIDi3772082.
KEGGidme:Dmel_CG33956.

Organism-specific databases

CTDi3772082.
FlyBaseiFBgn0001297. kay.

Phylogenomic databases

eggNOGiKOG1414. Eukaryota.
ENOG4111CH5. LUCA.
GeneTreeiENSGT00730000110541.
InParanoidiP21525.
KOiK09031.
OMAiYQNGHIM.
OrthoDBiEOG091G0FN2.
PhylomeDBiP21525.

Enzyme and pathway databases

ReactomeiR-DME-2559580. Oxidative Stress Induced Senescence.
R-DME-2871796. FCERI mediated MAPK activation.
R-DME-450341. Activation of the AP-1 family of transcription factors.

Miscellaneous databases

ChiTaRSikay. fly.
GenomeRNAii3772082.

Gene expression databases

BgeeiFBgn0001297.
ExpressionAtlasiP21525. baseline.
GenevisibleiP21525. DM.

Family and domain databases

InterProiIPR000837. AP-1.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR23351. PTHR23351. 1 hit.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
PRINTSiPR00042. LEUZIPPRFOS.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOSLA_DROME
AccessioniPrimary (citable) accession number: P21525
Secondary accession number(s): A8MPI0
, Q400M6, Q400M7, Q53YJ8, Q8T9E2, Q9BH37, Q9NBW7, Q9VAH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 16, 2009
Last modified: November 30, 2016
This is version 152 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Mammals typically have four copies of fos, Drosophila has a single gene with multiple transcription start sites giving rise to multiple protein isoforms.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.