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Protein

Transcription factor kayak, isoforms A/B/F

Gene

kay

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Developmentally regulated transcription factor AP-1 binds and recognizes the enhancer DNA sequence: 5'-TGA[CG]TCA-3'. May play a role in the function or determination of a particular subset of cells in the developing embryo. It is able to carry out its function either independently of or in conjunction with Jra.1 Publication

Miscellaneous

Mammals typically have four copies of fos, Drosophila has a single gene with multiple transcription start sites giving rise to multiple protein isoforms.

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • protein heterodimerization activity Source: FlyBase
  • protein serine/threonine kinase activity Source: Reactome
  • repressing transcription factor binding Source: FlyBase
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • adult chitin-containing cuticle pigmentation Source: FlyBase
  • antimicrobial humoral response Source: FlyBase
  • border follicle cell migration Source: FlyBase
  • collateral sprouting of injured axon Source: FlyBase
  • compound eye development Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • dorsal closure Source: FlyBase
  • embryo development ending in birth or egg hatching Source: UniProtKB
  • establishment of planar polarity Source: FlyBase
  • establishment or maintenance of cell polarity Source: BHF-UCL
  • germ cell development Source: FlyBase
  • imaginal disc fusion, thorax closure Source: FlyBase
  • JNK cascade Source: FlyBase
  • locomotor rhythm Source: FlyBase
  • ovarian follicle cell migration Source: FlyBase
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • R3/R4 cell fate commitment Source: FlyBase
  • regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle Source: FlyBase
  • response to wounding Source: FlyBase
  • second mitotic wave involved in compound eye morphogenesis Source: FlyBase
  • synaptic growth at neuromuscular junction Source: FlyBase
  • wing disc development Source: FlyBase
  • wound healing Source: FlyBase

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-209394 Transcriptional activtion and repression of REL-68 target genes
R-DME-209409 Formation of the nuclear AP-1 transcription factor 'scaffolding complex'
R-DME-209425 Transcriptional activtion by AP-1 transcription factor
R-DME-2559580 Oxidative Stress Induced Senescence
R-DME-2871796 FCERI mediated MAPK activation
R-DME-450341 Activation of the AP-1 family of transcription factors

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor kayak, isoforms A/B/F
Alternative name(s):
AP-1
Fos-related antigen
Short name:
Dfos
Short name:
dFra
Gene namesi
Name:kay
Synonyms:Fra
ORF Names:CG15509
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0001297 kay

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Loss of isoform A causes embryonic lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764781 – 755Transcription factor kayak, isoforms A/B/FAdd BLAST755

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei548Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21525
PRIDEiP21525

PTM databases

iPTMnetiP21525

Expressioni

Tissue specificityi

Early expression in the embryo is mesodermal and some of this expression is localized to a region surrounding the cephalic furrow. Later in embryonic development expression is ectodermal, corresponding to muscle attachment sites. Also observed in part of the mid- and hindgut and in the anal pad.1 Publication

Developmental stagei

Isoform A and isoform B are expressed both maternally and zygotically. Zygotically expressed throughout embryogenesis, until second larval instar. Isoform A is more highly expressed than isoform B.2 Publications

Gene expression databases

BgeeiFBgn0001297
ExpressionAtlasiP21525 baseline and differential
GenevisibleiP21525 DM

Interactioni

Subunit structurei

Homodimer. Heterodimer with Jra. The kay-Jra heterodimer binds more stably to the AP-1 site than either of the two proteins alone.

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • repressing transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi533679, 61 interactors
DIPiDIP-22245N
IntActiP21525, 2 interactors
STRINGi7227.FBpp0288515

Structurei

3D structure databases

ProteinModelPortaliP21525
SMRiP21525
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini418 – 481bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni420 – 439Basic motifPROSITE-ProRule annotationAdd BLAST20
Regioni446 – 453Leucine-zipperPROSITE-ProRule annotation8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi19 – 150Gln-richAdd BLAST132
Compositional biasi176 – 273Thr-richAdd BLAST98

Sequence similaritiesi

Belongs to the bZIP family. Fos subfamily.Curated

Phylogenomic databases

eggNOGiKOG1414 Eukaryota
ENOG4111CH5 LUCA
GeneTreeiENSGT00730000110541
InParanoidiP21525
KOiK09031
OMAiHMSTLMT
OrthoDBiEOG091G0FN2
PhylomeDBiP21525

Family and domain databases

InterProiView protein in InterPro
IPR000837 AP-1
IPR004827 bZIP
PANTHERiPTHR23351 PTHR23351, 2 hits
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
PRINTSiPR00042 LEUZIPPRFOS
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform F (identifier: P21525-5) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKNLNGRTHN ACYHPYYHQS LHFAQQQQQQ QQHHLQQQQQ HMQQQQQQQQ
60 70 80 90 100
APQQQLRHQQ RQLPTQPAYQ QSQSVAHNAF PLRSSSNNYG HVASSAYAAS
110 120 130 140 150
SGSHNSNNAA AMAAVCQMQN FFNQQQQQQQ QLEFNNNCMP INYYQQQQQQ
160 170 180 190 200
HYPSESQSSA SGWNPETPGQ AQLALTATTC NTTAAATCNT TAAATTSTTA
210 220 230 240 250
TSAAAGSDNN HSDNFAMDAS EIATFLANEL FLQQLGNFET GQSVLTLTTP
260 270 280 290 300
TLTPTTTRNI EDTLGHLLSD TQTDRVAGCA GFAVPKVLPN AIDVLGMGIP
310 320 330 340 350
TGVSSLPLQQ TFDLSLGQGS ESEDSNASYN DTQMNEEQDT TDTSSAHTDS
360 370 380 390 400
TSYQAGHIMA GSVNGGGVNN FSNVLAAVSS SRGSASVGSS NANTSNTPAR
410 420 430 440 450
RGGGRRPNRS TNMTPEEEQK RAVRRERNKQ AAARCRKRRV DQTNELTEEV
460 470 480 490 500
EQLEKRGESM RKEIEVLTNS KNQLEYLLAT HRATCQKIRS DMLSVVTCNG
510 520 530 540 550
LIAPAGLLSA GSSGSGASSH HNHNSNDSSN GTITGMDATL NSTGRSNSPL
560 570 580 590 600
DLKPAANIDS LLMHIKDEPL DGAIDSGSSL DQDGPPPSKR ITLPPMSTMP
610 620 630 640 650
HVHLSTILTP TGASSGSLQT PITSTAPGGF GSAFPVTSNG SSINNINSIG
660 670 680 690 700
NNMNSPTLNA HNKVPKERPN TLAFQRPLGQ MHLTMANNKA GGPTQIQGVP
710 720 730 740 750
IQTPSTGTFN FDSLMDGGTG LTPVSGPLVP NSSSTNKHPL ELPTPTAEPS

KLVSL
Note: No experimental confirmation available.
Length:755
Mass (Da):80,579
Last modified:June 16, 2009 - v4
Checksum:i6E21F0460B626DAA
GO
Isoform A (identifier: P21525-2) [UniParc]FASTAAdd to basket
Also known as: beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: MKNLNGRTHN...FLANELFLQQ → MKVKVERTTK...KIATPTRNLI

Note: Produced by alternative promoter usage.Curated
Show »
Length:595
Mass (Da):62,812
Checksum:i99B46F99444141A7
GO
Isoform B (identifier: P21525-3) [UniParc]FASTAAdd to basket
Also known as: gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: MKNLNGRTHN...FLANELFLQQ → MTLDSYNIFNDEYLFNMPLSPLPKV

Note: Produced by alternative promoter usage.
Show »
Length:546
Mass (Da):57,483
Checksum:iAAC27E0A7AC6F27F
GO
Isoform D (identifier: A8MPH9-1) [UniParc]FASTAAdd to basket
Also known as: alpha
The sequence of this isoform can be found in the external entry A8MPH9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative promoter usage.
Length:722
Mass (Da):78,320
GO
Isoform sro (identifier: A8MPH9-2) [UniParc]FASTAAdd to basket
Also known as: shroud
The sequence of this isoform can be found in the external entry A8MPH9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: May perform a regulatory function, acting as a dominant negative regulator of isoform alpha.
Length:210
Mass (Da):24,742
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti613A → P in CAA38082 (PubMed:2116361).Curated1
Isoform A (identifier: P21525-2)
Sequence conflicti19D → N in AAK11267 (PubMed:11470538).Curated1
Sequence conflicti19D → N in AAK11268 (PubMed:11470538).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0178911 – 234MKNLN…LFLQQ → MKVKVERTTKKPAIRKPEDP DPAEEDRVKMVQDDPEDQEN QAVDEEELDFLPADLSAAIS TATTKIATPTRNLI in isoform A. 3 PublicationsAdd BLAST234
Alternative sequenceiVSP_0178921 – 234MKNLN…LFLQQ → MTLDSYNIFNDEYLFNMPLS PLPKV in isoform B. 2 PublicationsAdd BLAST234

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54143 mRNA Translation: CAA38082.1
AF238310 mRNA Translation: AAF69496.1
AY574373 mRNA Translation: AAS87366.1
AH010388 mRNA Translation: AAK11268.2
AF332660 mRNA Translation: AAK11267.2
DQ858476 mRNA Translation: ABI74758.1
AE014297 Genomic DNA Translation: AAZ83992.1
AE014297 Genomic DNA Translation: AAZ83993.1
AE014297 Genomic DNA Translation: AAZ83994.2
AY069805 mRNA Translation: AAL39950.1
PIRiA35847
RefSeqiNP_001027577.2, NM_001032405.4 [P21525-5]
NP_001027578.1, NM_001032406.2 [P21525-3]
NP_001027579.1, NM_001032407.3 [P21525-2]
UniGeneiDm.6968

Genome annotation databases

EnsemblMetazoaiFBtr0099989; FBpp0084844; FBgn0001297 [P21525-2]
FBtr0099990; FBpp0084846; FBgn0001297 [P21525-3]
FBtr0290076; FBpp0288515; FBgn0001297 [P21525-5]
GeneIDi3772082
KEGGidme:Dmel_CG33956

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFOSLA_DROME
AccessioniPrimary (citable) accession number: P21525
Secondary accession number(s): A8MPI0
, Q400M6, Q400M7, Q53YJ8, Q8T9E2, Q9BH37, Q9NBW7, Q9VAH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 169 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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