Reviewed, UniProtKB/Swiss-Prot P21520 (SCA_DROME)

Names and origin

Protein names

Recommended name:
    Protein scabrous

Gene names

Name:	sca
ORF Names:	CG17579

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	799 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in regulation of neurogenesis. May encode a lateral inhibitor of R8 differentiation. In conjunction with Gp150, promotes Notch activation in response to Delta by regulating acquisition of insensitivity to Delta in a subset of cells. Ref.1 Ref.5
Subcellular location	Late endosome. Note: Colocalizes in late endosomes with Gp150. Ref.1 Ref.5
Post-translational modification	Possesses five pairs of dibasic residues that may be the target of proteolytic processing.
Sequence similarities	Contains 1 fibrinogen C-terminal domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Notch signaling pathway
Cellular component	Endosome
Domain	Signal
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW R8 cell fate commitment Traceable author statement. Source: FlyBase female meiosis chromosome segregation Inferred from mutant phenotype. Source: FlyBase imaginal disc-derived wing margin morphogenesis Inferred from mutant phenotype. Source: FlyBase lateral inhibition Non-traceable author statement. Source: FlyBase ommatidial rotation Inferred from mutant phenotype. Source: FlyBase regulation of R8 cell spacing in compound eye Traceable author statement. Source: FlyBase
Cellular component	extracellular region Non-traceable author statement. Source: FlyBase late endosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	receptor binding Inferred from electronic annotation. Source: InterPro signal transducer activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
alph	Q9VAK1	1	EBI-95026,EBI-207670

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 51	51	Potential
	Chain	52 – 799	748	Protein scabrous		PRO_0000009108
Regions
	Domain	533 – 737	205	Fibrinogen C-terminal
	Compositional bias	200 – 343	144	Gln-rich
Amino acid modifications
	Glycosylation	372	1	N-linked (GlcNAc...) Potential
	Glycosylation	587	1	N-linked (GlcNAc...) Potential
	Glycosylation	618	1	N-linked (GlcNAc...) Potential
	Glycosylation	660	1	N-linked (GlcNAc...) Potential
	Glycosylation	744	1	N-linked (GlcNAc...) Potential
	Glycosylation	787	1	N-linked (GlcNAc...) Potential
	Disulfide bond	542 ↔ 568		By similarity
	Disulfide bond	687 ↔ 700		By similarity
Experimental info
	Sequence conflict	301	1	G → C in AAA28880. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21520-1 [UniParc]. Last modified October 24, 2003. Version 3. Checksum: 87E1B4D60D4F5C29	FASTA	799	90,119
	10 20 30 40 50 60 MRDWQTFPDL QKKKVSRDHL NCPATMAGSN VLWPILLAVV LLQISVAFVS GAASGGVVLS 70 80 90 100 110 120 DVNNMLRDAK VVTSEKPVVH SKQETEAPES SVELLRFVDD DEDSEDISSI ERQDGRTMES 130 140 150 160 170 180 KKMADQVRLL TKQLNALMLR RREDYEMLEH NLRKSLRLTT NANSVDADMR SELNQLREEL 190 200 210 220 230 240 AALRSSQSGN KERLTVEWLQ QTISEIRKQL VDLQRTASNV AQDVQQRSST FEDLATIRSD 250 260 270 280 290 300 YQQLKLDLAA QRERQQQTEV YVQELREEML QQEQDFQHAL VKLQQRTRKD GSSASVEEES 310 320 330 340 350 360 GSQEANQEQT GLETTADHKR RHCRFQSEQI HQLQLAQRNL RRQVNGLRFH HIDERVRSIE 370 380 390 400 410 420 VEQHRIANAN FNLSSQIASL DKLHTSMLEL LEDVEGLQTK MDKSIPELRH EISKLEFANA 430 440 450 460 470 480 QITSEQSLIR EEGTNAARSL QAMAVSVSVL QEEREGMRKL SANVDQLRTN VDRLQSLVND 490 500 510 520 530 540 EMKNKLTHLN KPHKRPHHQN VQAQMPQDDS PIDSVLAETL VSELENVETQ YEAIINKLPH 550 560 570 580 590 600 DCSEVHTQTD GLHLIAPAGQ RHPLMTHCTA DGWTTVQRRF DGSADFNRSW ADYAQGFGAP 610 620 630 640 650 660 GGEFWIGNEQ LHHLTLDNCS RLQVQMQDIY DNVWVAEYKR FYISSRADGY RLHIAEYSGN 670 680 690 700 710 720 ASDALNYQQG MQFSAIDDDR DISQTHCAAN YEGGWWFSHC QHANLNGRYN LGLTWFDAAR 730 740 750 760 770 780 NEWIAVKSSR MLVKRLPAVE CQANASASGA FVSVSGSAAD AAPSSGATTT TTTATAAPAT 790 VTTPKTNNSV VQFVAAGQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Spacing differentiation in the developing Drosophila eye: a fibrinogen-related lateral inhibitor encoded by scabrous." Baker N.E., Mlodzik M., Rubin G.M. Science 250:1370-1377(1990) [PubMed: 2175046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Scabrous and Gp150 are endosomal proteins that regulate Notch activity." Li Y., Fetchko M., Lai Z.C., Baker N.E. Development 130:2819-2827(2003) [PubMed: 12756167] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60065 Genomic DNA. Translation: AAA28880.1. Different initiation. AE013599 Genomic DNA. Translation: AAF58455.2. AY129456 mRNA. Translation: AAM76198.1.
PIR	A39832.
RefSeq	NP_476710.2. NP_725230.2.
UniGene	Dm.4735
3D structure databases
HSSP	HSSP built from PDB template 1FZD based on UniProtKB P02671.
ModBase	Search...
Protein-protein interaction databases
IntAct	P21520. 1 interaction.
Genome annotation databases
Ensembl	FBgn0003326. Drosophila melanogaster. [Contig view]
GeneID	36411.
KEGG	dme:Dmel_CG17579.
Organism-specific databases
FlyBase	FBgn0003326. sca.
Phylogenomic databases
HOGENOM	P21520.
OMA	P21520. HRIANAN.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-004322-MON. DMEL-XXX-02:DMEL-XXX-02-004323-MON.
Gene expression databases
ArrayExpress	P21520.
GermOnline	CG17579. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002181. Fibrinogen_a/b/g_C. IPR014716. Fibrinogen_a/b/g_C_1. [Graphical view]
Gene3D	G3DSA:3.90.215.10. Fibrinogen_a/b/g_C_1. 1 hit.
Pfam	PF00147. Fibrinogen_C. 1 hit. [Graphical view]
SMART	SM00186. FBG. 1 hit. [Graphical view]
PROSITE	PS00514. FIBRINOGEN_C_1. 1 hit. PS51406. FIBRINOGEN_C_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	798423.

Entry information

Entry name

SCA_DROME

Accession

Primary (citable) accession number: P21520
Secondary accession number(s): Q0E998, Q8MQI8, Q9V6G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	October 24, 2003
Last modified:	June 16, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project