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Protein

Maltodextrin glucosidase

Gene

malZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in regulating the intracellular level of maltotriose. Cleaves glucose from the reducing end of maltotriose and longer maltodextrins with a chain length of up to 7 glucose units.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei336NucleophileBy similarity1
Active sitei373Proton donorBy similarity1
Sitei448Transition state stabilizerBy similarity1

GO - Molecular functioni

  • alpha-1,4-glucosidase activity Source: EcoCyc
  • maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  • alpha-glucan catabolic process Source: EcoCyc
  • cellular oligosaccharide catabolic process Source: EcoCyc

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processMaltose metabolism

Enzyme and pathway databases

BioCyciEcoCyc:MALTODEXGLUCOSID-MONOMER
MetaCyc:MALTODEXGLUCOSID-MONOMER
BRENDAi3.2.1.20 2026

Protein family/group databases

CAZyiCBM34 Carbohydrate-Binding Module Family 34
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Maltodextrin glucosidase (EC:3.2.1.20)
Alternative name(s):
Alpha-glucosidase
Gene namesi
Name:malZ
Ordered Locus Names:b0403, JW0393
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10565 malZ

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543341 – 604Maltodextrin glucosidaseAdd BLAST604

Proteomic databases

PaxDbiP21517
PRIDEiP21517

Expressioni

Inductioni

Under positive control of MalT.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi42632689 interactors.
DIPiDIP-10152N
IntActiP21517 8 interactors.
MINTiP21517
STRINGi316385.ECDH10B_0359

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5BN7X-ray3.70A1-604[»]
ProteinModelPortaliP21517
SMRiP21517
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4107R28 Bacteria
COG0366 LUCA
HOGENOMiHOG000055363
InParanoidiP21517
KOiK01187
OMAiTPDWVKH

Family and domain databases

CDDicd02857 E_set_CDase_PDE_N, 1 hit
Gene3Di2.60.40.11801 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR004185 Glyco_hydro_13_lg-like_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR014756 Ig_E-set
IPR017069 MalZ
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02903 Alpha-amylase_N, 1 hit
PIRSFiPIRSF036918 Maltodextrin_glucosidase, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

P21517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNAWHLPVP PFVKQSKDQL LITLWLTGED PPQRIMLRTE HDNEEMSVPM
60 70 80 90 100
HKQRSQPQPG VTAWRAAIDL SSGQPRRRYS FKLLWHDRQR WFTPQGFSRM
110 120 130 140 150
PPARLEQFAV DVPDIGPQWA ADQIFYQIFP DRFARSLPRE AEQDHVYYHH
160 170 180 190 200
AAGQEIILRD WDEPVTAQAG GSTFYGGDLD GISEKLPYLK KLGVTALYLN
210 220 230 240 250
PVFKAPSVHK YDTEDYRHVD PQFGGDGALL RLRHNTQQLG MRLVLDGVFN
260 270 280 290 300
HSGDSHAWFD RHNRGTGGAC HNPESPWRDW YSFSDDGTAL DWLGYASLPK
310 320 330 340 350
LDYQSESLVN EIYRGEDSIV RHWLKAPWNM DGWRLDVVHM LGEAGGARNN
360 370 380 390 400
MQHVAGITEA AKETQPEAYI VGEHFGDARQ WLQADVEDAA MNYRGFTFPL
410 420 430 440 450
WGFLANTDIS YDPQQIDAQT CMAWMDNYRA GLSHQQQLRM FNQLDSHDTA
460 470 480 490 500
RFKTLLGRDI ARLPLAVVWL FTWPGVPCIY YGDEVGLDGK NDPFCRKPFP
510 520 530 540 550
WQVEKQDTAL FALYQRMIAL RKKSQALRHG GCQVLYAEDN VVVFVRVLNQ
560 570 580 590 600
QRVLVAINRG EACEVVLPAS PFLNAVQWQC KEGHGQLTDG ILALPAISAT

VWMN
Length:604
Mass (Da):69,041
Last modified:November 16, 2011 - v5
Checksum:iB5A14754D7A42CCB
GO

Sequence cautioni

The sequence AAB40159 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE76183 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti301L → M in CAA42498 (PubMed:1918057).Curated1
Sequence conflicti445D → E in CAA42498 (PubMed:1918057).Curated1
Sequence conflicti546R → S in CAA42498 (PubMed:1918057).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59839 Genomic DNA Translation: CAA42498.1
U82664 Genomic DNA Translation: AAB40159.1 Different initiation.
U00096 Genomic DNA Translation: AAC73506.2
AP009048 Genomic DNA Translation: BAE76183.1 Different initiation.
M37702 Genomic DNA Translation: AAA16112.1
PIRiC64769
RefSeqiNP_414937.2, NC_000913.3
WP_000930752.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73506; AAC73506; b0403
BAE76183; BAE76183; BAE76183
GeneIDi949131
KEGGiecj:JW0393
eco:b0403
PATRICifig|511145.12.peg.419

Similar proteinsi

Entry informationi

Entry nameiMALZ_ECOLI
AccessioniPrimary (citable) accession number: P21517
Secondary accession number(s): Q2MC23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 16, 2011
Last modified: March 28, 2018
This is version 144 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome