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P21517 (MALZ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Maltodextrin glucosidase

EC=3.2.1.20
Alternative name(s):
Alpha-glucosidase
Gene names
Name:malZ
Ordered Locus Names:b0403, JW0393
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in regulating the intracellular level of maltotriose. Cleaves glucose from the reducing end of maltotriose and longer maltodextrins with a chain length of up to 7 glucose units.

Catalytic activity

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Subunit structure

Monomer.

Subcellular location

Cytoplasm.

Induction

Under positive control of MalT.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Sequence caution

The sequence AAB40159.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAE76183.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 604604Maltodextrin glucosidase
PRO_0000054334

Sites

Active site3361Nucleophile By similarity
Active site3731Proton donor By similarity
Site4481Transition state stabilizer By similarity

Experimental info

Sequence conflict3011L → M in CAA42498. Ref.1
Sequence conflict4451D → E in CAA42498. Ref.1
Sequence conflict5461R → S in CAA42498. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P21517 [UniParc].

Last modified November 16, 2011. Version 5.
Checksum: B5A14754D7A42CCB

FASTA60469,041
        10         20         30         40         50         60 
MLNAWHLPVP PFVKQSKDQL LITLWLTGED PPQRIMLRTE HDNEEMSVPM HKQRSQPQPG 

        70         80         90        100        110        120 
VTAWRAAIDL SSGQPRRRYS FKLLWHDRQR WFTPQGFSRM PPARLEQFAV DVPDIGPQWA 

       130        140        150        160        170        180 
ADQIFYQIFP DRFARSLPRE AEQDHVYYHH AAGQEIILRD WDEPVTAQAG GSTFYGGDLD 

       190        200        210        220        230        240 
GISEKLPYLK KLGVTALYLN PVFKAPSVHK YDTEDYRHVD PQFGGDGALL RLRHNTQQLG 

       250        260        270        280        290        300 
MRLVLDGVFN HSGDSHAWFD RHNRGTGGAC HNPESPWRDW YSFSDDGTAL DWLGYASLPK 

       310        320        330        340        350        360 
LDYQSESLVN EIYRGEDSIV RHWLKAPWNM DGWRLDVVHM LGEAGGARNN MQHVAGITEA 

       370        380        390        400        410        420 
AKETQPEAYI VGEHFGDARQ WLQADVEDAA MNYRGFTFPL WGFLANTDIS YDPQQIDAQT 

       430        440        450        460        470        480 
CMAWMDNYRA GLSHQQQLRM FNQLDSHDTA RFKTLLGRDI ARLPLAVVWL FTWPGVPCIY 

       490        500        510        520        530        540 
YGDEVGLDGK NDPFCRKPFP WQVEKQDTAL FALYQRMIAL RKKSQALRHG GCQVLYAEDN 

       550        560        570        580        590        600 
VVVFVRVLNQ QRVLVAINRG EACEVVLPAS PFLNAVQWQC KEGHGQLTDG ILALPAISAT 


VWMN 

« Hide

References

« Hide 'large scale' references
[1]"The malZ gene of Escherichia coli, a member of the maltose regulon, encodes a maltodextrin glucosidase."
Tapio S., Yeh F., Shuman H.A., Boos W.
J. Biol. Chem. 266:19450-19458(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes."
Reuter K., Slany R., Ullrich F., Kersten H.
J. Bacteriol. 173:2256-2264(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 191-604.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59839 Genomic DNA. Translation: CAA42498.1.
U82664 Genomic DNA. Translation: AAB40159.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73506.2.
AP009048 Genomic DNA. Translation: BAE76183.1. Different initiation.
M37702 Genomic DNA. Translation: AAA16112.1.
PIRC64769.
RefSeqNP_414937.2. NC_000913.3.
YP_488695.1. NC_007779.1.

3D structure databases

ProteinModelPortalP21517.
SMRP21517. Positions 42-601.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10152N.
IntActP21517. 8 interactions.
MINTMINT-1219352.
STRING511145.b0403.

Protein family/group databases

CAZyCBM34. Carbohydrate-Binding Module Family 34.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73506; AAC73506; b0403.
BAE76183; BAE76183; BAE76183.
GeneID12934274.
949131.
KEGGecj:Y75_p0391.
eco:b0403.
PATRIC32115955. VBIEscCol129921_0419.

Organism-specific databases

EchoBASEEB0560.
EcoGeneEG10565. malZ.

Phylogenomic databases

eggNOGCOG0366.
HOGENOMHOG000055363.
KOK01187.
OMAPYRRLRY.
ProtClustDBPRK10785.

Enzyme and pathway databases

BioCycEcoCyc:MALTODEXGLUCOSID-MONOMER.
ECOL316407:JW0393-MONOMER.
MetaCyc:MALTODEXGLUCOSID-MONOMER.

Gene expression databases

GenevestigatorP21517.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004185. Glyco_hydro_13_lg-like_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR014756. Ig_E-set.
IPR017069. MalZ.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02903. Alpha-amylase_N. 1 hit.
[Graphical view]
PIRSFPIRSF036918. Maltodextrin_glucosidase. 1 hit.
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

PROP21517.

Entry information

Entry nameMALZ_ECOLI
AccessionPrimary (citable) accession number: P21517
Secondary accession number(s): Q2MC23
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 16, 2011
Last modified: March 19, 2014
This is version 120 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene