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P21517

- MALZ_ECOLI

UniProt

P21517 - MALZ_ECOLI

Protein

Maltodextrin glucosidase

Gene

malZ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 5 (16 Nov 2011)
      Previous versions | rss
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    Functioni

    May play a role in regulating the intracellular level of maltotriose. Cleaves glucose from the reducing end of maltotriose and longer maltodextrins with a chain length of up to 7 glucose units.

    Catalytic activityi

    Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei336 – 3361NucleophileBy similarity
    Active sitei373 – 3731Proton donorBy similarity
    Sitei448 – 4481Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. alpha-1,4-glucosidase activity Source: EcoCyc
    2. cation binding Source: InterPro
    3. maltose alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. alpha-glucan catabolic process Source: EcoCyc
    2. cellular oligosaccharide catabolic process Source: EcoCyc
    3. maltose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Maltose metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:MALTODEXGLUCOSID-MONOMER.
    ECOL316407:JW0393-MONOMER.
    MetaCyc:MALTODEXGLUCOSID-MONOMER.

    Protein family/group databases

    CAZyiCBM34. Carbohydrate-Binding Module Family 34.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Maltodextrin glucosidase (EC:3.2.1.20)
    Alternative name(s):
    Alpha-glucosidase
    Gene namesi
    Name:malZ
    Ordered Locus Names:b0403, JW0393
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10565. malZ.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 604604Maltodextrin glucosidasePRO_0000054334Add
    BLAST

    Expressioni

    Inductioni

    Under positive control of MalT.

    Gene expression databases

    GenevestigatoriP21517.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    DIPiDIP-10152N.
    IntActiP21517. 8 interactions.
    MINTiMINT-1219352.
    STRINGi511145.b0403.

    Structurei

    3D structure databases

    ProteinModelPortaliP21517.
    SMRiP21517. Positions 42-601.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiCOG0366.
    HOGENOMiHOG000055363.
    KOiK01187.
    OMAiGNNDPFC.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004185. Glyco_hydro_13_lg-like_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR014756. Ig_E-set.
    IPR017069. MalZ.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02903. Alpha-amylase_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036918. Maltodextrin_glucosidase. 1 hit.
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P21517-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLNAWHLPVP PFVKQSKDQL LITLWLTGED PPQRIMLRTE HDNEEMSVPM    50
    HKQRSQPQPG VTAWRAAIDL SSGQPRRRYS FKLLWHDRQR WFTPQGFSRM 100
    PPARLEQFAV DVPDIGPQWA ADQIFYQIFP DRFARSLPRE AEQDHVYYHH 150
    AAGQEIILRD WDEPVTAQAG GSTFYGGDLD GISEKLPYLK KLGVTALYLN 200
    PVFKAPSVHK YDTEDYRHVD PQFGGDGALL RLRHNTQQLG MRLVLDGVFN 250
    HSGDSHAWFD RHNRGTGGAC HNPESPWRDW YSFSDDGTAL DWLGYASLPK 300
    LDYQSESLVN EIYRGEDSIV RHWLKAPWNM DGWRLDVVHM LGEAGGARNN 350
    MQHVAGITEA AKETQPEAYI VGEHFGDARQ WLQADVEDAA MNYRGFTFPL 400
    WGFLANTDIS YDPQQIDAQT CMAWMDNYRA GLSHQQQLRM FNQLDSHDTA 450
    RFKTLLGRDI ARLPLAVVWL FTWPGVPCIY YGDEVGLDGK NDPFCRKPFP 500
    WQVEKQDTAL FALYQRMIAL RKKSQALRHG GCQVLYAEDN VVVFVRVLNQ 550
    QRVLVAINRG EACEVVLPAS PFLNAVQWQC KEGHGQLTDG ILALPAISAT 600
    VWMN 604
    Length:604
    Mass (Da):69,041
    Last modified:November 16, 2011 - v5
    Checksum:iB5A14754D7A42CCB
    GO

    Sequence cautioni

    The sequence AAB40159.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence BAE76183.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti301 – 3011L → M in CAA42498. (PubMed:1918057)Curated
    Sequence conflicti445 – 4451D → E in CAA42498. (PubMed:1918057)Curated
    Sequence conflicti546 – 5461R → S in CAA42498. (PubMed:1918057)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59839 Genomic DNA. Translation: CAA42498.1.
    U82664 Genomic DNA. Translation: AAB40159.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73506.2.
    AP009048 Genomic DNA. Translation: BAE76183.1. Different initiation.
    M37702 Genomic DNA. Translation: AAA16112.1.
    PIRiC64769.
    RefSeqiNP_414937.2. NC_000913.3.
    YP_488695.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73506; AAC73506; b0403.
    BAE76183; BAE76183; BAE76183.
    GeneIDi12934274.
    949131.
    KEGGiecj:Y75_p0391.
    eco:b0403.
    PATRICi32115955. VBIEscCol129921_0419.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59839 Genomic DNA. Translation: CAA42498.1 .
    U82664 Genomic DNA. Translation: AAB40159.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC73506.2 .
    AP009048 Genomic DNA. Translation: BAE76183.1 . Different initiation.
    M37702 Genomic DNA. Translation: AAA16112.1 .
    PIRi C64769.
    RefSeqi NP_414937.2. NC_000913.3.
    YP_488695.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P21517.
    SMRi P21517. Positions 42-601.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10152N.
    IntActi P21517. 8 interactions.
    MINTi MINT-1219352.
    STRINGi 511145.b0403.

    Protein family/group databases

    CAZyi CBM34. Carbohydrate-Binding Module Family 34.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73506 ; AAC73506 ; b0403 .
    BAE76183 ; BAE76183 ; BAE76183 .
    GeneIDi 12934274.
    949131.
    KEGGi ecj:Y75_p0391.
    eco:b0403.
    PATRICi 32115955. VBIEscCol129921_0419.

    Organism-specific databases

    EchoBASEi EB0560.
    EcoGenei EG10565. malZ.

    Phylogenomic databases

    eggNOGi COG0366.
    HOGENOMi HOG000055363.
    KOi K01187.
    OMAi GNNDPFC.

    Enzyme and pathway databases

    BioCyci EcoCyc:MALTODEXGLUCOSID-MONOMER.
    ECOL316407:JW0393-MONOMER.
    MetaCyc:MALTODEXGLUCOSID-MONOMER.

    Miscellaneous databases

    PROi P21517.

    Gene expression databases

    Genevestigatori P21517.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004185. Glyco_hydro_13_lg-like_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR014756. Ig_E-set.
    IPR017069. MalZ.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02903. Alpha-amylase_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036918. Maltodextrin_glucosidase. 1 hit.
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The malZ gene of Escherichia coli, a member of the maltose regulon, encodes a maltodextrin glucosidase."
      Tapio S., Yeh F., Shuman H.A., Boos W.
      J. Biol. Chem. 266:19450-19458(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes."
      Reuter K., Slany R., Ullrich F., Kersten H.
      J. Bacteriol. 173:2256-2264(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 191-604.
      Strain: K12.

    Entry informationi

    Entry nameiMALZ_ECOLI
    AccessioniPrimary (citable) accession number: P21517
    Secondary accession number(s): Q2MC23
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: November 16, 2011
    Last modified: October 1, 2014
    This is version 122 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3