Reviewed, UniProtKB/Swiss-Prot P21515 (ACPH_ECOLI)

Names and origin

Protein names

Recommended name:
    Acyl carrier protein phosphodiesterase
      Short name=ACP phosphodiesterase
    EC=3.1.4.14

Gene names

Name:	acpH
Synonyms:	yajB
Ordered Locus Names:	b0404, JW0394

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts holo-ACP to apo-ACP by hydrolytic cleavage of the phosphopantetheine prosthetic group from ACP. Ref.5
Catalytic activity	Holo-[acyl-carrier-protein] + H2O = 4'-phosphopantetheine + apo-[acyl-carrier-protein]. HAMAP MF_01950
Sequence similarities	Belongs to the acpH family.
Sequence caution	The sequence AAA16113.1 differs from that shown. Reason: Frameshift at position 71.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	[acyl-carrier-protein] phosphodiesterase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Acyl carrier protein phosphodiesterase HAMAP MF_01950		PRO_0000168605

Sequences

	Sequence		Length	Mass (Da)
	P21515-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 0DE6F0CE01D6C523	FASTA	193	22,961
	10 20 30 40 50 60 MNFLAHLHLA HLAESSLSGN LLADFVRGNP EESFPPDVVA GIHMHRRIDV LTDNLPEVRE 70 80 90 100 110 120 AREWFRSETR RVAPITLDVM WDHFLSRHWS QLSPDFPLQE FVCYAREQVM TILPDSPPRF 130 140 150 160 170 180 INLNNYLWSE QWLVRYRDMD FIQNVLNGMA SRRPRLDALR DSWYDLDAHY DALETRFWQF 190 YPRMMAQASR KAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes." Reuter K., Slany R., Ullrich F., Kersten H. J. Bacteriol. 173:2256-2264(1991) [PubMed: 1706703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The enigmatic acyl carrier protein phosphodiesterase of Escherichia coli: genetic and enzymological characterization." Thomas J., Cronan J.E. J. Biol. Chem. 280:34675-34683(2005) [PubMed: 16107329] [Abstract] Cited for: FUNCTION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M37702 Genomic DNA. Translation: AAA16113.1. Frameshift. U82664 Genomic DNA. Translation: AAB40160.1. U00096 Genomic DNA. Translation: AAC73507.1. AP009048 Genomic DNA. Translation: BAE76184.1.
PIR	D64769.
RefSeq	AP_001054.1. NP_414938.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	949132.
GenomeReviews	Gene locus JW0394 in contig AP009048_GR. Gene locus b0404 in contig U00096_GR.
KEGG	ecj:JW0394. eco:b0404.
Organism-specific databases
EchoBASE	EB1087.
EcoGene	EG11095. acpH.
CMR	Search...
Phylogenomic databases
HOGENOM	P21515.
OMA	P21515. TFYPQLM.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11095-MON.
Family and domain databases
HAMAP	MF_01950. [Tree]
InterPro	IPR007431. ACP_phosphodiesterase. [Graphical view]
Pfam	PF04336. DUF479. 1 hit. [Graphical view]
PIRSF	PIRSF011489. DUF479. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACPH_ECOLI

Accession

Primary (citable) accession number: P21515
Secondary accession number(s): P46123, P77369, Q2MC22

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)