P21515 (ACPH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl carrier protein phosphodiesterase
Short name=ACP phosphodiesterase
EC=3.1.4.14

Gene names

Name:	acpH
Synonyms:	yajB
Ordered Locus Names:	b0404, JW0394

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts holo-ACP to apo-ACP by hydrolytic cleavage of the phosphopantetheine prosthetic group from ACP. Ref.5
Catalytic activity	Holo-[acyl-carrier-protein] + H2O = 4'-phosphopantetheine + apo-[acyl-carrier-protein]. HAMAP-Rule MF_01950
Sequence similarities	Belongs to the AcpH family.
Sequence caution	The sequence AAA16113.1 differs from that shown. Reason: Frameshift at position 71.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from direct assay Ref.5. Source: EcoCyc
Molecular_function	[acyl-carrier-protein] phosphodiesterase activity Inferred from direct assay PubMed 4290442. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Acyl carrier protein phosphodiesterase HAMAP-Rule MF_01950		PRO_0000168605

Sequences

	Sequence		Length	Mass (Da)
	P21515 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 0DE6F0CE01D6C523	FASTA	193	22,961
	10 20 30 40 50 60 MNFLAHLHLA HLAESSLSGN LLADFVRGNP EESFPPDVVA GIHMHRRIDV LTDNLPEVRE 70 80 90 100 110 120 AREWFRSETR RVAPITLDVM WDHFLSRHWS QLSPDFPLQE FVCYAREQVM TILPDSPPRF 130 140 150 160 170 180 INLNNYLWSE QWLVRYRDMD FIQNVLNGMA SRRPRLDALR DSWYDLDAHY DALETRFWQF 190 YPRMMAQASR KAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes." Reuter K., Slany R., Ullrich F., Kersten H. J. Bacteriol. 173:2256-2264(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The enigmatic acyl carrier protein phosphodiesterase of Escherichia coli: genetic and enzymological characterization." Thomas J., Cronan J.E. J. Biol. Chem. 280:34675-34683(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37702 Genomic DNA. Translation: AAA16113.1. Frameshift. U82664 Genomic DNA. Translation: AAB40160.1. U00096 Genomic DNA. Translation: AAC73507.1. AP009048 Genomic DNA. Translation: BAE76184.1.
PIR	D64769.
RefSeq	NP_414938.1. NC_000913.2. YP_488696.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P21515.
ModBase	Search...
Protein-protein interaction databases
IntAct	P21515. 2 interactions.
STRING	511145.b0404.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73507; AAC73507; b0404. BAE76184; BAE76184; BAE76184.
GeneID	12932723. 949132.
KEGG	ecj:Y75_p0392. eco:b0404.
PATRIC	32115957. VBIEscCol129921_0420.
Organism-specific databases
EchoBASE	EB1087.
EcoGene	EG11095. acpH.
Phylogenomic databases
eggNOG	COG3124.
HOGENOM	HOG000275880.
KO	K08682.
OMA	WLHRQID.
ProtClustDB	PRK10045.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11095-MONOMER. ECOL316407:JW0394-MONOMER. MetaCyc:EG11095-MONOMER.
BRENDA	3.1.4.14. 2026.
Gene expression databases
Genevestigator	P21515.
Family and domain databases
HAMAP	MF_01950. AcpH.
InterPro	IPR007431. ACP_phosphodiesterase. IPR023491. ACP_phosphodiesterase_gpbac. [Graphical view]
Pfam	PF04336. DUF479. 1 hit. [Graphical view]
PIRSF	PIRSF011489. DUF479. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACPH_ECOLI

Accession

Primary (citable) accession number: P21515
Secondary accession number(s): P46123, P77369, Q2MC22

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program