Reviewed, UniProtKB/Swiss-Prot P21472 (RS12_BACSU)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S12
      Short name=BS12

Gene names

Name:	rpsL
Synonyms:	fun, strA
Ordered Locus Names:	BSU01100

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	With S4 and S5 plays an important role in translational accuracy. HAMAP MF_00403 Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit By similarity.
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex By similarity.
Miscellaneous	Many streptomycin resistant B.subtilis with mutations in this protein produce increased quantities of a natural (but uncharacterized) antibiotic. HAMAP MF_00403 A number of substitutions and deletions can promote streptomycin resistance, dependence or pseudodependence; all but one of these (K55R) are associated with hyperaccurate translation. HAMAP MF_00403
Sequence similarities	Belongs to the ribosomal protein S12P family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: HAMAP
Cellular component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 138	137	30S ribosomal protein S12 HAMAP MF_00403		PRO_0000146178
Amino acid modifications
	Modified residue	102	1	3-methylthioaspartic acid By similarity
Natural variations
	Natural variant	56	1	K → I in RPSL4 (STRA1); confers streptomycin resistance and hyperaccurate translation, has no effect on natural antibiotic production.
	Natural variant	56	1	K → R in RPSL1 (KO-267); confers streptomycin resistance but not hyperaccurate translation, increases natural antibiotic production 10-fold.
Experimental info
	Mutagenesis	56	1	K → D: Confers streptomycin resistance and very poor growth in rich medium.
	Sequence conflict	102	1	D → N in BAA11001. Ref.1
	Sequence conflict	105	1	G → R in BAA11001. Ref.1
	Sequence conflict	128	1	S → P in BAA11001. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21472-1 [UniParc]. Last modified June 16, 2009. Version 5. Checksum: 1E8EDCEC75B75648	FASTA	138	15,216
	10 20 30 40 50 60 MPTINQLIRK GRVSKVENSK SPALNKGYNS FKKEHTNVSS PQKRGVCTRV GTMTPKKPNS 70 80 90 100 110 120 ALRKYARVRL TNGIEVTAYI PGIGHNLQEH SVVLIRGGRV KDLPGVRYHI VRGALDTAGV 130 ENRAQGRSKY GTKKPKAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome." Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H. Microbiology 142:3039-3046(1996) [PubMed: 8969501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 102; 105 AND 128.
[4]	"Genetic and transcriptional organization of the region encoding the beta subunit of Bacillus subtilis RNA polymerase." Boor K.J., Duncan M.L., Price C.W. J. Biol. Chem. 270:20329-20336(1995) [PubMed: 7657605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32. Strain: 168 / Marburg.
[5]	"Purification and characterization of 30S ribosomal proteins from Bacillus subtilis: correlation to Escherichia coli 30S proteins." Higo K., Otaka E., Osawa S. Mol. Gen. Genet. 185:239-244(1982) [PubMed: 6806564] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21.
[6]	"Acquisition of certain streptomycin-resistant (str) mutations enhances antibiotic production in bacteria." Hosoya Y., Okamoto S., Muramatsu H., Ochi K. Antimicrob. Agents Chemother. 42:2041-2047(1998) [PubMed: 9687404] [Abstract] Cited for: VARIANTS STREPTOMYCIN RESISTANT, ANTIBIOTIC PRODUCTION. Strain: 168.
[7]	"Construction of an in vivo nonsense readthrough assay system and functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus subtilis." Inaoka T., Kasai K., Ochi K. J. Bacteriol. 183:4958-4963(2001) [PubMed: 11489846] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS, VARIANTS STREPTOMYCIN RESISTANT. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D64127 Genomic DNA. Translation: BAA11001.1. AL009126 Genomic DNA. Translation: CAB11886.2. L43593 Genomic DNA. Translation: AAB59114.1.
PIR	C69700.
RefSeq	NP_387991.2.
3D structure databases
HSSP	HSSP built from PDB template 1J5E based on UniProtKB P17293.
SMR	P21472. Positions 15-137.
ModBase	Search...
Genome annotation databases
GeneID	936616.
GenomeReviews	Gene locus BSU01100 in contig AL009126_GR.
KEGG	bsu:BSU01100.
NMPDR	fig|224308.1.peg.110.
Organism-specific databases
SubtiList	BG19009. rpsL. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P21472.
OMA	SPALKSC.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0110-MON.
Family and domain databases
HAMAP	MF_00403. [Tree]
InterPro	IPR012340. NA-bd_OB-fold. IPR006032. Ribosomal_S12/S23. IPR005679. Ribosomal_S12_bac-type. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR11652:SF1. Ribosom_S12_bac. 1 hit. PTHR11652. Ribosomal_S12_23. 1 hit.
Pfam	PF00164. Ribosomal_S12. 1 hit. [Graphical view]
PIRSF	PIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTS	PR01034. RIBOSOMALS12.
TIGRFAMs	TIGR00981. rpsL_bact. 1 hit.
PROSITE	PS00055. RIBOSOMAL_S12. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RS12_BACSU

Accession

Primary (citable) accession number: P21472

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	June 16, 2009
Last modified:	November 3, 2009
This is version 88 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)