Reviewed, UniProtKB/Swiss-Prot P21467 (RS5_BACSU)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S5
      Short name=BS5

Gene names

Name:	rpsE
Synonyms:	spcA
Ordered Locus Names:	BSU01330

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	With S4 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations). HAMAP MF_01307
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S4 and S8 By similarity. HAMAP MF_01307
Domain	The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity. HAMAP MF_01307
Miscellaneous	Altered S5 proteins have been identified in a number of mutants. Some mutations in S5 have been shown to increase translational error frequencies. HAMAP MF_01307
Sequence similarities	Belongs to the ribosomal protein S5P family. Contains 1 S5 DRBM domain.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: HAMAP
Cellular component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	30S ribosomal protein S5 HAMAP MF_01307		PRO_0000131470
Regions
	Domain	11 – 74	64	S5 DRBM
Natural variations
	Natural variant	24 – 26	3	Missing in 1A241; spectinomycin resistant.
	Natural variant	26	1	K → I in BSPC 111; spectinomycin resistant.
	Natural variant	28	1	G → V in rpsE1; spectinomycin restistant, not a ram mutant.
	Natural variant	30	1	Missing in BPSC 31 and BPSC 61; spectinomycin resistant.
	Natural variant	104	1	G → R in rpsE9; suppresses S12 mutation K55D. Lethal in the absence of the S12 mutation.
	Natural variant	112	1	R → C in rpsE8; suppresses S12 mutant K55D. RspE8 is lethal in the absence of the S12 mutation.
	Natural variant	112	1	R → H in rpsE7; suppresses S12 mutant K55D. A ram mutation.

Sequences

	Sequence		Length	Mass (Da)
	P21467-1 [UniParc]. Last modified November 1, 1995. Version 3. Checksum: 5D96AA53FF892AF4	FASTA	166	17,623
	10 20 30 40 50 60 MRRIDPSKLE LEERLVTVNR VAKVVKGGRR FRFAALVVVG DKNGHVGFGT GKAQEVPEAI 70 80 90 100 110 120 RKAVEDAKKN LIEVPMVGTT IPHEIIGRFG AGNILLKPAS EGTGVIAGGP VRAVLELAGV 130 140 150 160 ADILSKSLGS NTPINMIRAT LQGLSELKRA EDVAKLRGKS VEELLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region." Suh J.-W., Boylan S.A., Oh S.H., Price C.W. Gene 169:17-23(1996) [PubMed: 8635744] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NCIB 3610.
[2]	"Sequence of the Bacillus subtilis spectinomycin resistance gene region." Yoshikawa H., Doi R.H. Nucleic Acids Res. 18:1647-1647(1990) [PubMed: 2139212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SPECTINOMYCIN RESISTANT. Strain: 1A241.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome." Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H. Microbiology 142:3039-3046(1996) [PubMed: 8969501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39. Strain: 168.
[5]	"Purification and characterization of 30S ribosomal proteins from Bacillus subtilis: correlation to Escherichia coli 30S proteins." Higo K., Otaka E., Osawa S. Mol. Gen. Genet. 185:239-244(1982) [PubMed: 6806564] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-40.
[6]	"Alteration of ribosomal protein S5 from two spectinomycin-resistant mutants of Bacillus subtilis: deletion of one amino acid residue." Higo K., Itoh T., Osawa S. Mol. Gen. Genet. 185:205-206(1982) [PubMed: 6211592] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-46, VARIANTS SPECTINOMYCIN RESISTANT BPSC 31 AND BPSC 61. Strain: ATCC 6633 / PCI 219 / NRS 231.
[7]	"Construction of an in vivo nonsense readthrough assay system and functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus subtilis." Inaoka T., Kasai K., Ochi K. J. Bacteriol. 183:4958-4963(2001) [PubMed: 11489846] [Abstract] Cited for: CHARACTERIZATION, VARIANTS. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L47971 Genomic DNA. Translation: AAB06816.1. M31102 Genomic DNA. Translation: AAB59115.1. Different initiation. AL009126 Genomic DNA. Translation: CAB11909.1. D64125 Genomic DNA. Translation: BAA20856.1.
PIR	R3BS5S. D69699.
RefSeq	NP_388014.1.
3D structure databases
SMR	P21467. Positions 4-148.
ModBase	Search...
Genome annotation databases
GeneID	938012.
GenomeReviews	Gene locus BSU01330 in contig AL009126_GR.
KEGG	bsu:BSU01330.
NMPDR	fig|224308.1.peg.133.
Organism-specific databases
SubtiList	BG10442. rpsE. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299153.
OMA	TLGIQDV.
PhylomeDB	P21467.
Family and domain databases
HAMAP	MF_01307_B. Ribosomal_S5_B. [Tree]
InterPro	IPR014720. dsRNA-bd-like. IPR000851. Ribosomal_S5. IPR005712. Ribosomal_S5_bac-type. IPR005324. Ribosomal_S5_C. IPR020568. Ribosomal_S5_D2-typ_fold. IPR013810. Ribosomal_S5_N. IPR018192. Ribosomal_S5_N_CS. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit.
PANTHER	PTHR13718. Ribosomal_S5. 1 hit.
Pfam	PF00333. Ribosomal_S5. 1 hit. PF03719. Ribosomal_S5_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01021. rpsE_bact. 1 hit.
PROSITE	PS00585. RIBOSOMAL_S5. 1 hit. PS50881. S5_DSRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RS5_BACSU

Accession

Primary (citable) accession number: P21467

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)