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Protein

Thyrotropin receptor

Gene

Tshr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).

GO - Molecular functioni

  • thyroid-stimulating hormone receptor activity Source: RGD

GO - Biological processi

  • positive regulation of cell proliferation Source: RGD
  • thyroid-stimulating hormone signaling pathway Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Thyrotropin receptor
Alternative name(s):
Thyroid-stimulating hormone receptor
Short name:
TSH-R
Gene namesi
Name:Tshr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3911. Tshr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 413392ExtracellularSequence analysisAdd
BLAST
Transmembranei414 – 44128Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini442 – 4509CytoplasmicSequence analysis
Transmembranei451 – 47323Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini474 – 49421ExtracellularSequence analysisAdd
BLAST
Transmembranei495 – 51723Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini518 – 53720CytoplasmicSequence analysisAdd
BLAST
Transmembranei538 – 56023Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini561 – 58020ExtracellularSequence analysisAdd
BLAST
Transmembranei581 – 60222Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini603 – 62523CytoplasmicSequence analysisAdd
BLAST
Transmembranei626 – 64924Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini650 – 66011ExtracellularSequence analysisAdd
BLAST
Transmembranei661 – 68222Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini683 – 76482CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane raft Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi623 – 6231A → E or K: Loss of TSH-increased inositol phosphate, but not cAMP formation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 764743Thyrotropin receptorPRO_0000012788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 41PROSITE-ProRule annotation
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence analysis
Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence analysis
Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence analysis
Disulfide bondi494 ↔ 569PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP21463.
PRIDEiP21463.

PTM databases

PhosphoSiteiP21463.

Interactioni

Subunit structurei

Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005671.

Structurei

3D structure databases

ProteinModelPortaliP21463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati100 – 12425LRR 1Add
BLAST
Repeati125 – 15026LRR 2Add
BLAST
Repeati151 – 17424LRR 3Add
BLAST
Repeati176 – 19924LRR 4Add
BLAST
Repeati201 – 22323LRR 5Add
BLAST
Repeati225 – 24824LRR 6Add
BLAST
Repeati264 – 28825LRR 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi762 – 7643PDZ-binding

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.PROSITE-ProRule annotation
Contains 7 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2087. Eukaryota.
ENOG410XR1T. LUCA.
HOGENOMiHOG000045902.
HOVERGENiHBG003521.
InParanoidiP21463.
KOiK04249.
PhylomeDBiP21463.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002131. Gphrmn_rcpt_fam.
IPR032675. L_dom-like.
IPR026906. LRR_5.
IPR002274. TSH_rcpt.
[Graphical view]
PANTHERiPTHR24372. PTHR24372. 1 hit.
PTHR24372:SF0. PTHR24372:SF0. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
PF13306. LRR_5. 2 hits.
[Graphical view]
PRINTSiPR00373. GLYCHORMONER.
PR00237. GPCRRHODOPSN.
PR01145. TSHRECEPTOR.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPGSLLQLT LLLALPRSLW GRGCTSPPCE CHQEDDFRVT CKELHQIPSL
60 70 80 90 100
PPSTQTLKLI ETHLKTIPSL AFSSLPNISR IYLSIDATLQ RLEPHSFYNL
110 120 130 140 150
SKMTHIEIRN TRSLTYIDPD ALTELPLLKF LGIFNTGLRI FPDLTKIYST
160 170 180 190 200
DVFFILEITD NPYMTSVPEN AFQGLCNETL TLKLYNNGFT SIQGHAFNGT
210 220 230 240 250
KLDAVYLNKN KYLTAIDKDA FGGVYSGPTL LDVSSTSVTA LPSKGLEHLK
260 270 280 290 300
ELIAKNTWTL KKLPLSLSFL HLTRADLSYP SHCCAFKNQK KIRGILESLM
310 320 330 340 350
CNESSIRNLR QRKSVNVMRG PVYQEYEEGL GDNHVGYKQN SKFQEGPSNS
360 370 380 390 400
HYYVFFEEQE DEIIGFGQEL KNPQEETLQA FDSHYDYTVC GDNEDMVCTP
410 420 430 440 450
KSDEFNPCED IMGYKFLRIV VWFVSPMALL GNVFVLFVLL TSHYKLTVPR
460 470 480 490 500
FLMCNLAFAD FCMGVYLLLI ASVDLYTHTE YYNHAIDWQT GPGCNTAGFF
510 520 530 540 550
TVFASELSVY TLTVITLERW YAITFAMRLD RKIRLRHAYT IMAGGWVSCF
560 570 580 590 600
LLALLPMVGI SSYAKVSICL PMDTDTPLAL AYIALVLLLN VVAFVIVCSC
610 620 630 640 650
YVKIYITVRN PQYNPRDKDT KIAKRMAVLI FTDFMCMAPI SFYALSALMN
660 670 680 690 700
KPLITVTNSG VLLVLFYPLN SCANPFLYAI FTKAFQRDVF ILLSKFGLCK
710 720 730 740 750
HQAQAYQAQR VCPNNNTGIQ IQKIPQDTRQ SLPNVQDTYE PLGSSHLTPK
760
LQGRISEEYT QTAL
Length:764
Mass (Da):86,475
Last modified:November 1, 1995 - v2
Checksum:i79FC45CC9D26167B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34842 mRNA. Translation: AAA42302.1.
PIRiA35956.
RefSeqiNP_037020.1. NM_012888.1.
UniGeneiRn.87913.

Genome annotation databases

GeneIDi25360.
KEGGirno:25360.
UCSCiRGD:3911. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34842 mRNA. Translation: AAA42302.1.
PIRiA35956.
RefSeqiNP_037020.1. NM_012888.1.
UniGeneiRn.87913.

3D structure databases

ProteinModelPortaliP21463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005671.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP21463.

Proteomic databases

PaxDbiP21463.
PRIDEiP21463.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25360.
KEGGirno:25360.
UCSCiRGD:3911. rat.

Organism-specific databases

CTDi7253.
RGDi3911. Tshr.

Phylogenomic databases

eggNOGiKOG2087. Eukaryota.
ENOG410XR1T. LUCA.
HOGENOMiHOG000045902.
HOVERGENiHBG003521.
InParanoidiP21463.
KOiK04249.
PhylomeDBiP21463.

Miscellaneous databases

NextBioi606337.
PROiP21463.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002131. Gphrmn_rcpt_fam.
IPR032675. L_dom-like.
IPR026906. LRR_5.
IPR002274. TSH_rcpt.
[Graphical view]
PANTHERiPTHR24372. PTHR24372. 1 hit.
PTHR24372:SF0. PTHR24372:SF0. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
PF13306. LRR_5. 2 hits.
[Graphical view]
PRINTSiPR00373. GLYCHORMONER.
PR00237. GPCRRHODOPSN.
PR01145. TSHRECEPTOR.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, chromosomal assignment, and regulation of the rat thyrotropin receptor: expression of the gene is regulated by thyrotropin, agents that increase cAMP levels, and thyroid autoantibodies."
    Akamizu T., Ikuyama S., Saji M., Kosugi S., Kozak C., McBride O.W., Kohn L.D.
    Proc. Natl. Acad. Sci. U.S.A. 87:5677-5681(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mutation of alanine 623 in the third cytoplasmic loop of the rat thyrotropin (TSH) receptor results in a loss in the phosphoinositide but not cAMP signal induced by TSH and receptor autoantibodies."
    Kosugi S., Okajima F., Ban T., Hidaka A., Shenker A., Kohn L.D.
    J. Biol. Chem. 267:24153-24156(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ALA-623.

Entry informationi

Entry nameiTSHR_RAT
AccessioniPrimary (citable) accession number: P21463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.