Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thyrotropin receptor

Gene

Tshr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed:1696008). Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin (PubMed:12045258). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (By similarity). Plays a central role in controlling thyroid cell metabolism (PubMed:12045258).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Thyrotropin receptor
Alternative name(s):
Thyroid-stimulating hormone receptor
Short name:
TSH-R
Gene namesi
Name:Tshr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3911 Tshr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 413ExtracellularSequence analysisAdd BLAST392
Transmembranei414 – 441Helical; Name=1Sequence analysisAdd BLAST28
Topological domaini442 – 450CytoplasmicSequence analysis9
Transmembranei451 – 473Helical; Name=2Sequence analysisAdd BLAST23
Topological domaini474 – 494ExtracellularSequence analysisAdd BLAST21
Transmembranei495 – 517Helical; Name=3Sequence analysisAdd BLAST23
Topological domaini518 – 537CytoplasmicSequence analysisAdd BLAST20
Transmembranei538 – 560Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini561 – 580ExtracellularSequence analysisAdd BLAST20
Transmembranei581 – 602Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini603 – 625CytoplasmicSequence analysisAdd BLAST23
Transmembranei626 – 649Helical; Name=6Sequence analysisAdd BLAST24
Topological domaini650 – 660ExtracellularSequence analysisAdd BLAST11
Transmembranei661 – 682Helical; Name=7Sequence analysisAdd BLAST22
Topological domaini683 – 764CytoplasmicSequence analysisAdd BLAST82

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi623A → E or K: Loss of TSH-increased inositol phosphate, but not cAMP formation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001278822 – 764Thyrotropin receptorAdd BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 41PROSITE-ProRule annotation
Glycosylationi77N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi177N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi198N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi302N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei385SulfotyrosineBy similarity1
Disulfide bondi494 ↔ 569PROSITE-ProRule annotation

Post-translational modificationi

Glycosylated.By similarity
Sulfated. Sulfation on Tyr-385 plays a role in thyrotropin receptor binding and activation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbiP21463
PRIDEiP21463

PTM databases

iPTMnetiP21463
PhosphoSitePlusiP21463

Interactioni

Subunit structurei

Interacts with heterodimer GPHA2:GPHB5; this interaction stimulates cAMP production. Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function.By similarity

Protein-protein interaction databases

IntActiP21463, 1 interactor
STRINGi10116.ENSRNOP00000005671

Structurei

3D structure databases

ProteinModelPortaliP21463
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati100 – 124LRR 1Add BLAST25
Repeati125 – 150LRR 2Add BLAST26
Repeati151 – 174LRR 3Add BLAST24
Repeati176 – 199LRR 4Add BLAST24
Repeati201 – 223LRR 5Add BLAST23
Repeati225 – 248LRR 6Add BLAST24
Repeati264 – 288LRR 7Add BLAST25

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi762 – 764PDZ-binding3

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2087 Eukaryota
ENOG410XR1T LUCA
HOGENOMiHOG000045902
HOVERGENiHBG003521
InParanoidiP21463
KOiK04249
PhylomeDBiP21463

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR002131 Gphrmn_rcpt_fam
IPR026906 LRR_5
IPR032675 LRR_dom_sf
IPR002274 TSH_rcpt
IPR034298 TSHR/LHCGR/FSHR
PANTHERiPTHR24372 PTHR24372, 1 hit
PTHR24372:SF0 PTHR24372:SF0, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PF13306 LRR_5, 2 hits
PRINTSiPR00373 GLYCHORMONER
PR00237 GPCRRHODOPSN
PR01145 TSHRECEPTOR
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPGSLLQLT LLLALPRSLW GRGCTSPPCE CHQEDDFRVT CKELHQIPSL
60 70 80 90 100
PPSTQTLKLI ETHLKTIPSL AFSSLPNISR IYLSIDATLQ RLEPHSFYNL
110 120 130 140 150
SKMTHIEIRN TRSLTYIDPD ALTELPLLKF LGIFNTGLRI FPDLTKIYST
160 170 180 190 200
DVFFILEITD NPYMTSVPEN AFQGLCNETL TLKLYNNGFT SIQGHAFNGT
210 220 230 240 250
KLDAVYLNKN KYLTAIDKDA FGGVYSGPTL LDVSSTSVTA LPSKGLEHLK
260 270 280 290 300
ELIAKNTWTL KKLPLSLSFL HLTRADLSYP SHCCAFKNQK KIRGILESLM
310 320 330 340 350
CNESSIRNLR QRKSVNVMRG PVYQEYEEGL GDNHVGYKQN SKFQEGPSNS
360 370 380 390 400
HYYVFFEEQE DEIIGFGQEL KNPQEETLQA FDSHYDYTVC GDNEDMVCTP
410 420 430 440 450
KSDEFNPCED IMGYKFLRIV VWFVSPMALL GNVFVLFVLL TSHYKLTVPR
460 470 480 490 500
FLMCNLAFAD FCMGVYLLLI ASVDLYTHTE YYNHAIDWQT GPGCNTAGFF
510 520 530 540 550
TVFASELSVY TLTVITLERW YAITFAMRLD RKIRLRHAYT IMAGGWVSCF
560 570 580 590 600
LLALLPMVGI SSYAKVSICL PMDTDTPLAL AYIALVLLLN VVAFVIVCSC
610 620 630 640 650
YVKIYITVRN PQYNPRDKDT KIAKRMAVLI FTDFMCMAPI SFYALSALMN
660 670 680 690 700
KPLITVTNSG VLLVLFYPLN SCANPFLYAI FTKAFQRDVF ILLSKFGLCK
710 720 730 740 750
HQAQAYQAQR VCPNNNTGIQ IQKIPQDTRQ SLPNVQDTYE PLGSSHLTPK
760
LQGRISEEYT QTAL
Length:764
Mass (Da):86,475
Last modified:November 1, 1995 - v2
Checksum:i79FC45CC9D26167B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34842 mRNA Translation: AAA42302.1
PIRiA35956
RefSeqiNP_037020.1, NM_012888.1
UniGeneiRn.87913

Genome annotation databases

GeneIDi25360
KEGGirno:25360
UCSCiRGD:3911 rat

Similar proteinsi

Entry informationi

Entry nameiTSHR_RAT
AccessioniPrimary (citable) accession number: P21463
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health