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Protein

E3 ubiquitin-protein ligase sina

Gene

sina

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that is required for specification of R7 photoreceptor cell fate in the eye by mediating the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk) (PubMed:9267026, PubMed:9267027, PubMed:11526076, PubMed:12215542, PubMed:18160715). E3 Ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:12215542). Acts via the formation of a complex with ebi and phyl that ubiquitinates the transcription repressor ttk, a general inhibitor of photoreceptor differentiation, in a subset of photoreceptor cells in the eye, leading to the differentiation of cells into neurons (PubMed:9267026, PubMed:9267027, PubMed:11526076, PubMed:12215542). Also involved in external sensory organ development (PubMed:11526076).5 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi130Zinc 1By similarity1
Metal bindingi137Zinc 1By similarity1
Metal bindingi149Zinc 1By similarity1
Metal bindingi153Zinc 1By similarity1
Metal bindingi160Zinc 2By similarity1
Metal bindingi167Zinc 2By similarity1
Metal bindingi179Zinc 2By similarity1
Metal bindingi184Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri73 – 108RING-typePROSITE-ProRule annotationAdd BLAST36
Zinc fingeri125 – 185SIAH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein self-association Source: FlyBase
  • ubiquitin-protein transferase activity Source: FlyBase
  • zinc ion binding Source: FlyBase

GO - Biological processi

  • enteroendocrine cell differentiation Source: FlyBase
  • proteasomal protein catabolic process Source: FlyBase
  • proteolysis Source: FlyBase
  • R7 cell fate commitment Source: FlyBase
  • regulation of R7 cell differentiation Source: UniProtKB
  • response to stimulus Source: UniProtKB-KW
  • sensory organ development Source: FlyBase
  • ubiquitin-dependent protein catabolic process Source: FlyBase
  • visual perception Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processSensory transduction, Ubl conjugation pathway, Vision
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-373752 Netrin-1 signaling
R-DME-5689880 Ub-specific processing proteases
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase sina (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase sinaCurated
Seven in absentia protein
Gene namesi
Name:sina
ORF Names:CG9949
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0003410 sina

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561751 – 314E3 ubiquitin-protein ligase sinaAdd BLAST314

Proteomic databases

PaxDbiP21461
PRIDEiP21461

Expressioni

Tissue specificityi

In many ommatidial precursor cells.

Gene expression databases

BgeeiFBgn0003410
ExpressionAtlasiP21461 baseline and differential
GenevisibleiP21461 DM

Interactioni

Subunit structurei

Component of some E3 complex at least composed of sina, ebi and phyl. Interacts with eff.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein self-association Source: FlyBase

Protein-protein interaction databases

BioGridi65183, 61 interactors
DIPiDIP-17392N
IntActiP21461, 48 interactors
STRINGi7227.FBpp0075091

Structurei

3D structure databases

ProteinModelPortaliP21461
SMRiP21461
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 314SBDAdd BLAST193

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 21Poly-Ala8
Compositional biasi26 – 55Ser/Thr-richAdd BLAST30
Compositional biasi46 – 55Poly-Ser10

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.By similarity
The SBD domain (substrate-binding domain) mediates the interaction with substrate proteins. It is related to the TRAF family.By similarity

Sequence similaritiesi

Belongs to the SINA (Seven in absentia) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri73 – 108RING-typePROSITE-ProRule annotationAdd BLAST36
Zinc fingeri125 – 185SIAH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
InParanoidiP21461
KOiK04506
OMAiEACEFRP
OrthoDBiEOG091G0BPY
PhylomeDBiP21461

Family and domain databases

Gene3Di2.60.210.10, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit

Sequencei

Sequence statusi: Complete.

P21461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKINPKRR EPTAAAAGAG ATGVATNTST STGSSSAGNT SSANTSSSSS
60 70 80 90 100
SSLSSAGGGD AGMSADLTSL FECPVCFDYV LPPILQCSSG HLVCVSCRSK
110 120 130 140 150
LTCCPTCRGP LANIRNLAME KVASNVKFPC KHSGYGCTAS LVYTEKTEHE
160 170 180 190 200
ETCECRPYLC PCPGASCKWQ GPLDLVMQHL MMSHKSITTL QGEDIVFLAT
210 220 230 240 250
DINLPGAVDW VMMQSCFGHH FMLVLEKQEK YDGHQQFFAI VQLIGSRKEA
260 270 280 290 300
ENFVYRLELN GNRRRLTWEA MPRSIHEGVA SAIHNSDCLV FDTSIAQLFA
310
DNGNLGINVT ISLV
Length:314
Mass (Da):33,707
Last modified:November 1, 1995 - v2
Checksum:iB58D7D5E8DA2F958
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38384 mRNA Translation: AAA28901.1
AE014296 Genomic DNA Translation: AAF49403.1
AE014296 Genomic DNA Translation: AAN11744.1
AY060358 mRNA Translation: AAL25397.1
PIRiA36195
RefSeqiNP_001287089.1, NM_001300160.1
NP_476725.1, NM_057377.4
NP_730206.1, NM_168689.2
UniGeneiDm.7276

Genome annotation databases

EnsemblMetazoaiFBtr0075332; FBpp0075091; FBgn0003410
FBtr0075333; FBpp0075092; FBgn0003410
FBtr0344230; FBpp0310636; FBgn0003410
GeneIDi39884
KEGGidme:Dmel_CG9949

Similar proteinsi

Entry informationi

Entry nameiSINA_DROME
AccessioniPrimary (citable) accession number: P21461
Secondary accession number(s): A4V214, Q9VVB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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