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Protein

Recoverin

Gene

RCVRN

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi74 – 851; low affinityAdd BLAST12
Calcium bindingi110 – 1212; high affinityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Recoverin
Alternative name(s):
p26
Gene namesi
Name:RCVRN
Synonyms:RCV1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000737582 – 202RecoverinAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Post-translational modificationi

The N-terminal glycine is linked to one of four different types of acyl groups. The most abundant is myristoleate (14:1), but 14:0, 14:2, and 12:0 acyl residues are also present. Myristoylation is necessary for recoverin to bind photoreceptor membranes when the calcium concentration is greater than one micromolar.1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP21457.
PRIDEiP21457.

Expressioni

Tissue specificityi

Retina and pineal gland.

Gene expression databases

BgeeiENSBTAG00000025088.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GRK1P283272EBI-8592784,EBI-7865560

Protein-protein interaction databases

IntActiP21457. 4 interactors.
MINTiMINT-1507490.
STRINGi9913.ENSBTAP00000034949.

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 20Combined sources10
Helixi25 – 38Combined sources14
Beta strandi42 – 45Combined sources4
Helixi46 – 56Combined sources11
Beta strandi58 – 60Combined sources3
Helixi63 – 73Combined sources11
Beta strandi78 – 81Combined sources4
Helixi83 – 94Combined sources12
Helixi98 – 100Combined sources3
Helixi102 – 109Combined sources8
Beta strandi114 – 117Combined sources4
Helixi119 – 130Combined sources12
Helixi135 – 138Combined sources4
Beta strandi139 – 141Combined sources3
Helixi143 – 145Combined sources3
Helixi148 – 158Combined sources11
Beta strandi162 – 164Combined sources3
Beta strandi166 – 168Combined sources3
Helixi169 – 178Combined sources10
Helixi180 – 186Combined sources7
Helixi190 – 196Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IKUNMR-A2-202[»]
1JSANMR-A2-202[»]
1LA3NMR-A2-202[»]
1OMRX-ray1.50A2-202[»]
1OMVX-ray1.90A2-202[»]
1RECX-ray1.90A2-202[»]
2HETX-ray3.00A/B/C/D2-190[»]
2I94NMR-A1-202[»]
4M2OX-ray1.50A2-197[»]
4M2PX-ray1.45A2-202[»]
4M2QX-ray1.90A2-202[»]
4MLWX-ray1.45A2-202[»]
4YI8X-ray1.20A2-202[»]
4YI9X-ray1.35A2-202[»]
ProteinModelPortaliP21457.
SMRiP21457.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21457.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 59EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini61 – 96EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini97 – 132EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini147 – 182EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP21457.
KOiK13764.
OMAiKECPSGR.
OrthoDBiEOG091G11T4.
TreeFamiTF300009.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNSKSGALS KEILEELQLN TKFTEEELSS WYQSFLKECP SGRITRQEFQ
60 70 80 90 100
TIYSKFFPEA DPKAYAQHVF RSFDANSDGT LDFKEYVIAL HMTSAGKTNQ
110 120 130 140 150
KLEWAFSLYD VDGNGTISKN EVLEIVTAIF KMISPEDTKH LPEDENTPEK
160 170 180 190 200
RAEKIWGFFG KKDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKLKEK

KL
Length:202
Mass (Da):23,333
Last modified:January 23, 2007 - v3
Checksum:i75504DEBE8288BA8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19L → Q AA sequence (PubMed:1672637).Curated1
Sequence conflicti21T → N AA sequence (PubMed:1672637).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95858 mRNA. Translation: AAB59256.1.
X63322 mRNA. Translation: CAA44928.1.
PIRiA46129.
RefSeqiNP_776590.1. NM_174165.2.
UniGeneiBt.56904.

Genome annotation databases

EnsembliENSBTAT00000035069; ENSBTAP00000034949; ENSBTAG00000025088.
GeneIDi281447.
KEGGibta:281447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95858 mRNA. Translation: AAB59256.1.
X63322 mRNA. Translation: CAA44928.1.
PIRiA46129.
RefSeqiNP_776590.1. NM_174165.2.
UniGeneiBt.56904.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IKUNMR-A2-202[»]
1JSANMR-A2-202[»]
1LA3NMR-A2-202[»]
1OMRX-ray1.50A2-202[»]
1OMVX-ray1.90A2-202[»]
1RECX-ray1.90A2-202[»]
2HETX-ray3.00A/B/C/D2-190[»]
2I94NMR-A1-202[»]
4M2OX-ray1.50A2-197[»]
4M2PX-ray1.45A2-202[»]
4M2QX-ray1.90A2-202[»]
4MLWX-ray1.45A2-202[»]
4YI8X-ray1.20A2-202[»]
4YI9X-ray1.35A2-202[»]
ProteinModelPortaliP21457.
SMRiP21457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP21457. 4 interactors.
MINTiMINT-1507490.
STRINGi9913.ENSBTAP00000034949.

Proteomic databases

PaxDbiP21457.
PRIDEiP21457.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000035069; ENSBTAP00000034949; ENSBTAG00000025088.
GeneIDi281447.
KEGGibta:281447.

Organism-specific databases

CTDi5957.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP21457.
KOiK13764.
OMAiKECPSGR.
OrthoDBiEOG091G11T4.
TreeFamiTF300009.

Enzyme and pathway databases

ReactomeiR-BTA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

EvolutionaryTraceiP21457.

Gene expression databases

BgeeiENSBTAG00000025088.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECO_BOVIN
AccessioniPrimary (citable) accession number: P21457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds two calcium ions; one with high affinity, the other with low affinity.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.