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P21452 (NK2R_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Substance-K receptor

Short name=SKR
Alternative name(s):
NK-2 receptor
Short name=NK-2R
Neurokinin A receptor
Tachykinin receptor 2
Gene names
Name:TACR2
Synonyms:NK2R, NKNAR, TAC2R
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is a receptor for the tachykinin neuropeptide substance K (neurokinin A). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance K > neuromedin-K > substance P. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processexcretion

Traceable author statement PubMed 10862829. Source: ProtInc

intestine smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

muscle contraction

Traceable author statement Ref.1. Source: ProtInc

negative regulation of luteinizing hormone secretion

Inferred from electronic annotation. Source: Ensembl

operant conditioning

Inferred from electronic annotation. Source: Ensembl

positive regulation of acetylcholine secretion, neurotransmission

Inferred from electronic annotation. Source: Ensembl

positive regulation of uterine smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

positive regulation of vascular permeability

Inferred from electronic annotation. Source: Ensembl

prolactin secretion

Inferred from electronic annotation. Source: Ensembl

tachykinin receptor signaling pathway

Traceable author statement PubMed 8307161. Source: ProtInc

   Cellular_componentintegral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 23597562. Source: IntAct

substance K receptor activity

Inferred from electronic annotation. Source: Ensembl

tachykinin receptor activity

Traceable author statement PubMed 8307161. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TAC1P203662EBI-6655413,EBI-6655449

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Substance-K receptor
PRO_0000069893

Regions

Topological domain1 – 3232Extracellular Potential
Transmembrane33 – 5624Helical; Name=1; Potential
Topological domain57 – 6913Cytoplasmic Potential
Transmembrane70 – 9021Helical; Name=2; Potential
Topological domain91 – 10717Extracellular Potential
Transmembrane108 – 12922Helical; Name=3; Potential
Topological domain130 – 14920Cytoplasmic Potential
Transmembrane150 – 17021Helical; Name=4; Potential
Topological domain171 – 19626Extracellular Potential
Transmembrane197 – 21822Helical; Name=5; Potential
Topological domain219 – 25133Cytoplasmic Potential
Transmembrane252 – 27221Helical; Name=6; Potential
Topological domain273 – 29018Extracellular Potential
Transmembrane291 – 31020Helical; Name=7; Potential
Topological domain311 – 39888Cytoplasmic Potential

Amino acid modifications

Lipidation3241S-palmitoyl cysteine Potential
Glycosylation111N-linked (GlcNAc...) Potential
Glycosylation191N-linked (GlcNAc...) Potential
Disulfide bond106 ↔ 181 By similarity

Natural variations

Natural variant231I → T. Ref.4 Ref.5
Corresponds to variant rs5030920 [ dbSNP | Ensembl ].
VAR_016159
Natural variant471A → T.
Corresponds to variant rs57500981 [ dbSNP | Ensembl ].
VAR_061221
Natural variant2451M → K. Ref.1 Ref.4 Ref.5 Ref.8 Ref.9 Ref.10 Ref.11
Corresponds to variant rs55953810 [ dbSNP | Ensembl ].
VAR_061222
Natural variant3631T → A.
Corresponds to variant rs57900755 [ dbSNP | Ensembl ].
VAR_061223
Natural variant3751R → H. Ref.6
Corresponds to variant rs2229170 [ dbSNP | Ensembl ].
VAR_014680
Natural variant3951H → R.
Corresponds to variant rs58692969 [ dbSNP | Ensembl ].
VAR_061224

Experimental info

Sequence conflict381T → P in AAC31760. Ref.1
Sequence conflict381T → P in AAA60347. Ref.1
Sequence conflict401Y → V Ref.4
Sequence conflict2641L → P in AAH96842. Ref.11
Sequence conflict2931F → FF Ref.6
Sequence conflict3401P → R in AAH96842. Ref.11

Sequences

Sequence LengthMass (Da)Tools
P21452 [UniParc].

Last modified May 5, 2009. Version 3.
Checksum: 20AD618EF74263A7

FASTA39844,442
        10         20         30         40         50         60 
MGTCDIVTEA NISSGPESNT TGITAFSMPS WQLALWATAY LALVLVAVTG NAIVIWIILA 

        70         80         90        100        110        120 
HRRMRTVTNY FIVNLALADL CMAAFNAAFN FVYASHNIWY FGRAFCYFQN LFPITAMFVS 

       130        140        150        160        170        180 
IYSMTAIAAD RYMAIVHPFQ PRLSAPSTKA VIAGIWLVAL ALASPQCFYS TVTMDQGATK 

       190        200        210        220        230        240 
CVVAWPEDSG GKTLLLYHLV VIALIYFLPL AVMFVAYSVI GLTLWRRAVP GHQAHGANLR 

       250        260        270        280        290        300 
HLQAMKKFVK TMVLVVLTFA ICWLPYHLYF ILGSFQEDIY CHKFIQQVYL ALFWLAMSST 

       310        320        330        340        350        360 
MYNPIIYCCL NHRFRSGFRL AFRCCPWVTP TKEDKLELTP TTSLSTRVNR CHTKETLFMA 

       370        380        390 
GDTAPSEATS GEAGRPQDGS GLWFGYGLLA PTKTHVEI 

« Hide

References

« Hide 'large scale' references
[1]"The human neurokinin A (substance K) receptor. Molecular cloning of the gene, chromosome localization, and isolation of cDNA from tracheal and gastric tissues."
Gerard N.P., Eddy R.L. Jr., Shows T.B., Gerard C.
J. Biol. Chem. 265:20455-20462(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT LYS-245.
Tissue: Stomach and Trachea.
[2]Erratum
Gerard N.P., Eddy R.L. Jr., Shows T.B., Gerard C.
J. Biol. Chem. 266:1354-1354(1991) [PubMed] [Europe PMC] [Abstract]
[3]"Molecular cloning of the human neurokinin-2 receptor cDNA by polymerase chain reaction and isolation of the gene."
Gerard N.P., Gerard C.
Ann. N. Y. Acad. Sci. 632:389-390(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Cloning, expression of the human substance K receptor, and analysis of its role in mitogenesis."
Cyr C., South V., Saltzman A., Felder S., Ricca G.A., Jaye M., Huebner K., Kagan J., Croce C.M., Schlessinger J.
Ann. N. Y. Acad. Sci. 632:426-427(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANTS THR-23 AND LYS-245.
Tissue: Jejunum.
[5]"Isolation and characterisation of the human lung NK-2 receptor gene using rapid amplification of cDNA ends."
Graham A., Hopkins B., Powell S.J., Danks P., Briggs I.
Biochem. Biophys. Res. Commun. 177:8-16(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-23 AND LYS-245.
Tissue: Lung.
[6]"Cloning and expression of the human substance K receptor and analysis of its role in mitogenesis."
Kris R.M., South V., Saltzman A., Felder S., Ricca G.A., Jaye M., Huebner K., Kagan J., Croce C.M., Schlessinger J.
Cell Growth Differ. 2:15-22(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-375.
[7]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-245.
Tissue: Small intestine.
[9]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Kopatz S.A., Aronstam R.S., Sharma S.V.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-245.
Tissue: Stomach.
[10]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LYS-245.
[11]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-245.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57414 mRNA. Translation: AAC31760.1.
M60284 expand/collapse EMBL AC list , M57415, M60281, M60282, M60283 Genomic DNA. Translation: AAA60347.1.
M75105 expand/collapse EMBL AC list , M75101, M75102, M75103, M75104 Genomic DNA. Translation: AAB05897.1.
AB065731 Genomic DNA. Translation: BAC05952.1.
AK291996 mRNA. Translation: BAF84685.1.
AY322545 mRNA. Translation: AAP84358.1.
CH471083 Genomic DNA. Translation: EAW54324.1.
BC096842 mRNA. Translation: AAH96842.1.
CCDSCCDS7293.1.
PIRJQ1059.
RefSeqNP_001048.2. NM_001057.2.
UniGeneHs.88372.

3D structure databases

ProteinModelPortalP21452.
SMRP21452. Positions 9-347.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112728. 2 interactions.
IntActP21452. 1 interaction.
STRING9606.ENSP00000362403.

Chemistry

BindingDBP21452.
ChEMBLCHEMBL2327.
DrugBankDB00575. Clonidine.
DB00104. Octreotide.
GuidetoPHARMACOLOGY361.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP21452.

Polymorphism databases

DMDM229462950.

Proteomic databases

PaxDbP21452.
PRIDEP21452.

Protocols and materials databases

DNASU6865.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000373306; ENSP00000362403; ENSG00000075073.
GeneID6865.
KEGGhsa:6865.
UCSCuc001jpn.2. human.

Organism-specific databases

CTD6865.
GeneCardsGC10M071163.
H-InvDBHIX0008887.
HGNCHGNC:11527. TACR2.
HPAHPA012084.
MIM162321. gene.
neXtProtNX_P21452.
PharmGKBPA36303.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG331485.
HOGENOMHOG000013018.
HOVERGENHBG103412.
InParanoidP21452.
KOK04223.
OMALCMAAFN.
PhylomeDBP21452.
TreeFamTF315303.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP21452.
BgeeP21452.
CleanExHS_TACR2.
GenevestigatorP21452.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001681. Neurokn_rcpt.
IPR000913. NK2_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR01025. NEUROKININ2R.
PR00244. NEUROKININR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTachykinin_receptor_2.
GenomeRNAi6865.
NextBio26793.
PROP21452.
SOURCESearch...

Entry information

Entry nameNK2R_HUMAN
AccessionPrimary (citable) accession number: P21452
Secondary accession number(s): A8K7I1 expand/collapse secondary AC list , Q4QRI5, Q8NGQ8, Q9UDE6, Q9UDE7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 5, 2009
Last modified: July 9, 2014
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries