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Reviewed, UniProtKB/Swiss-Prot P21449 (MDR2_CRIGR)

Last modified October 13, 2009. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Multidrug resistance protein 2
    EC=3.6.3.44
Alternative name(s):
    P-glycoprotein 2
Gene names
Name: PGY2
Synonyms: PGP2
OrganismCricetulus griseus (Chinese hamster)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

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Protein attributes

Sequence length1276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

PGP isoforms differ in their drug transport capabilities: PGP1 and PGP2 can mediate MDR, while PGP3 apparently cannot.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

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Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   DomainRepeat
Transmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12761276Multidrug resistance protein 2
PRO_0000093340

Regions

Topological domain1 – 5151Cytoplasmic Potential
Transmembrane52 – 7221 Potential
Transmembrane119 – 13921 Potential
Transmembrane188 – 20821 Potential
Transmembrane215 – 23521 Potential
Transmembrane296 – 31621 Potential
Transmembrane325 – 34521 Potential
Topological domain346 – 708363Cytoplasmic Potential
Transmembrane709 – 72921 Potential
Transmembrane755 – 77521 Potential
Transmembrane831 – 85121 Potential
Transmembrane852 – 87221 Potential
Transmembrane935 – 95521 Potential
Transmembrane972 – 99221 Potential
Topological domain993 – 1276284Cytoplasmic Potential
Domain51 – 356306ABC transmembrane type-1 1
Domain391 – 627237ABC transporter 1
Domain709 – 998290ABC transmembrane type-1 2
Domain1033 – 1271239ABC transporter 2
Nucleotide binding426 – 4338ATP 1 Potential
Nucleotide binding1068 – 10758ATP 2 Potential

Amino acid modifications

Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation8071N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
P21449-1 [UniParc].

Last modified November 1, 1991. Version 2.
Checksum: 5096B1385628812D

FASTA1,276141,058
        10         20         30         40         50         60 
MEFEEDFSAR ADKDFLKMGR KSKKEKKEKE NPNVGIFGMF RYADWLDKLY MVLGTLAAVL 

        70         80         90        100        110        120 
HGTSLPLLML VFGNMTDSFT KAETSIWPNM TNQSEINNTE VISGSLEEDM ATYAYYYTGI 

       130        140        150        160        170        180 
GAGVLIVAYI QVSFWCLAAG RQINKIRQKF FHAIMNQEIG WFDVHDIGEL NTRLTDDVSK 

       190        200        210        220        230        240 
INDGIGDKIG MFFQSIATFL AAFIVGFISG WKLTLVILAV SPLIGLSSAM WAKVLTSFTN 

       250        260        270        280        290        300 
KELQAYAKAG AVAEEVLAAI RTVIAFGGQN KELERYNKNL EEAKNVGIKK AVTANISIGI 

       310        320        330        340        350        360 
AYLLVYASYA LAFWYGTSLV LSNEYSVGQV LTVFFSILFG TFSIGHIAPN IEVFANARGA 

       370        380        390        400        410        420 
AYEIFKIIDN EPSIDSFSTQ GHKPDSVMGN LEFKNVHFSY PSRSGIKILK GLNLKVQSGQ 

       430        440        450        460        470        480 
TVALVGKSGC GKSTTVQLLQ RLYDPTEGVV SIDGQDIRTI NVRYLREIIG VVSQEPVLFA 

       490        500        510        520        530        540 
TTIAENIRYG RENVTMDEIE KAVKEANAYD FIMKLPHKFD TLVGERGAQL SGGQKQRIAI 

       550        560        570        580        590        600 
ARALVRNPKI LLLDEATSAL DTESEAVVQA ALDKAREGRT TIVIAHRLST VRNADVIAGF 

       610        620        630        640        650        660 
DGGVIVEQGN HEELMKEKGI YCRLVMMQTR GNEVELGSEA DGSQSDTIAS ELTSEEFKSP 

       670        680        690        700        710        720 
SVRKSTCRSI CGSQDQERRV SVKEAQDEDV PLVSFWGILK LNITEWPYLV VGVLCAVING 

       730        740        750        760        770        780 
CMQPVFSIVF SGIIGVFTRD DDPKTKQQNC NLFSLFFLVM GMICFVTYFF QGFTFGKAGE 

       790        800        810        820        830        840 
ILTKRLRYMV FKSMLRQDIS WFDDHRNSTG ALTTRLASDA ANVKGAMSSR LAGITQNVAN 

       850        860        870        880        890        900 
LGTGIIISLV YGWQLTLLLV VIAPLIILSG MMEMKVLSGQ ALKDKKELEV SGKIATEAIE 

       910        920        930        940        950        960 
NFRTVVSLTR EQKFENMYAQ SLQIPYRNAL KKAHVFGITF SFTQAMMYFS YAACFRFGAY 

       970        980        990       1000       1010       1020 
LVAHQIMTFE NVMLVFSAVV FGAIAAGNAS SFAPDYAKAK VSASHIIRIM EKIPSIDSYS 

      1030       1040       1050       1060       1070       1080 
TRGLKPNWLE GNVKFNEVVF NYPTRPDIPV LQGLSLEVKK GQTLALVGSS GCGKSTVVQL 

      1090       1100       1110       1120       1130       1140 
LERFYDPMAG TVFLDGKEIK QLNVQWLRAH LGIVSQEPIL FDCSIAENIA YGDNSRVVSQ 

      1150       1160       1170       1180       1190       1200 
DEIERAAKEA NIHQFIESLP DKYNTRVGDK GTQLSGGQKQ RIAIARALVR QPHILLLDEA 

      1210       1220       1230       1240       1250       1260 
TSALDTESEK VVQEALDKAR EGRTCIVIAH RLSTIQNADL IVVIQNGKVK EHGTHQQLLA 

      1270 
QKGIYFSMVQ AGAKRL

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References

[1]"Complete cDNA sequences encoding the Chinese hamster P-glycoprotein gene family."
Endicott J.A., Sarangi F., Ling V.
DNA Seq. 2:89-101(1991) [PubMed: 1685679] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Simultaneous expression of two P-glycoprotein genes in drug-sensitive Chinese hamster ovary cells."
Endicott J.A., Juranka P.F., Sarangi F., Gerlach J.H., Deuchars K.L., Ling V.
Mol. Cell. Biol. 7:4075-4081(1987) [PubMed: 2893255] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 622-1276.

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Cross-references

Sequence databases

M60041 mRNA. Translation: AAA68884.1.
M17896 mRNA. Translation: AAA37007.1.
PIRDVHY2C. B27126.

3D structure databases

HSSPHSSP built from PDB template 1MT0 based on UniProtKB P08716.
ModBaseSearch...

Phylogenomic databases

HOVERGENP21449.

Enzyme and pathway databases

BRENDA3.6.3.44. 18.

Family and domain databases

InterProIPR001140. ABC_TM_transpt.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
ProDomPD000006. ABC_transporter. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDR2_CRIGR
AccessionPrimary (citable) accession number: P21449
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1991
Last modified: October 13, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents