Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multidrug resistance protein 1

Gene

ABCB1

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Miscellaneous

PGP isoforms differ in their drug transport capabilities: PGP1 and PGP2 can mediate MDR, while PGP3 apparently cannot.

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi424 – 431ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1067 – 1074ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein 1 (EC:3.6.3.44)
Alternative name(s):
ATP-binding cassette sub-family B member 1
P-glycoprotein 1
CD_antigen: CD243
Gene namesi
Name:ABCB1
Synonyms:PGP1, PGY1
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaCricetidaeCricetinaeCricetulus
Proteomesi
  • UP000001075 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43CytoplasmicBy similarityAdd BLAST43
Transmembranei44 – 66HelicalPROSITE-ProRule annotationAdd BLAST23
Topological domaini67 – 113ExtracellularBy similarityAdd BLAST47
Transmembranei114 – 134HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini135 – 183CytoplasmicBy similarityAdd BLAST49
Transmembranei184 – 205HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini206 – 212ExtracellularBy similarity7
Transmembranei213 – 233HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini234 – 291CytoplasmicBy similarityAdd BLAST58
Transmembranei292 – 313HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini314 – 327ExtracellularBy similarityAdd BLAST14
Transmembranei328 – 349HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini350 – 708CytoplasmicBy similarityAdd BLAST359
Transmembranei709 – 729HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini730 – 753ExtracellularBy similarityAdd BLAST24
Transmembranei754 – 774HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini775 – 829CytoplasmicBy similarityAdd BLAST55
Transmembranei830 – 850HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini851ExtracellularBy similarity1
Transmembranei852 – 871HelicalPROSITE-ProRule annotationAdd BLAST20
Topological domaini872 – 931CytoplasmicBy similarityAdd BLAST60
Transmembranei932 – 954HelicalPROSITE-ProRule annotationAdd BLAST23
Topological domaini955 – 970ExtracellularBy similarityAdd BLAST16
Transmembranei971 – 992HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini993 – 1276CytoplasmicBy similarityAdd BLAST284

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933391 – 1276Multidrug resistance protein 1Add BLAST1276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi87N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi91N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi96N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei657PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Expressed at a higher level in intestines than in kidney and liver.1 Publication

Interactioni

Subunit structurei

Interacts with PSMB5.Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.By similarity

Structurei

Secondary structure

11276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1211 – 1220Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MVUX-ray1.78P1210-1222[»]
2AP2X-ray2.40P/Q1210-1223[»]
ProteinModelPortaliP21448.
SMRiP21448.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21448.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 354ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST305
Domaini389 – 625ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini708 – 997ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST290
Domaini1032 – 1270ABC transporter 2PROSITE-ProRule annotationAdd BLAST239

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG080809.
InParanoidiP21448.
KOiK05658.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
SMARTiView protein in SMART
SM00382. AAA. 2 hits.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

P21448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFEEDFSGR KDKNFLKMGR KSKKEKKEKK PVVSVFTMFR YAGWLDRLYM
60 70 80 90 100
LVGTLAAIIH GVALPLMMLV FGDMTDSFAS VGNIPTNATN NATQVNASDI
110 120 130 140 150
FGKLEEEMTT YAYYYTGIGA GVLIVAYIQV SFWCLAAGRQ IHKIRQKFFH
160 170 180 190 200
AIMNQEIGWF DVHDVGELNT RLTDDVSKIN EGIGDKIGMF FQAMATFFGG
210 220 230 240 250
FIIGFTRGWK LTLVILAISP VLGLSAGIWA KILSSFTDKE LQAYAKAGAV
260 270 280 290 300
AEEVLAAIRT VIAFGGQKKE LERYNNNLEE AKRLGIKKAI TANISMGAAF
310 320 330 340 350
LLIYASYALA FWYGTSLVIS KEYSIGQVLT VFFAVLIGAF SIGQASPNIE
360 370 380 390 400
AFANARGAAY EIFNIIDNKP SIDSFSKNGY KPDNIKGNLE FKNIHFSYPS
410 420 430 440 450
RKDVQILKGL NLKVQSGQTV ALVGNSGCGK STTVQLLQRL YDPTEGVVSI
460 470 480 490 500
DGQDIRTINV RYLREIIGVV SQEPVLFATT IAENIRYGRE NVTMDEIEKA
510 520 530 540 550
VKEANAYDFI MKLPHKFDTL VGERGAQLSG GQKQRIAIAR ALVRNPKILL
560 570 580 590 600
LDEATSALDT ESEAVVQAAL DKAREGRTTI VIAHRLSTVR NADIIAGFDG
610 620 630 640 650
GVIVEQGNHE ELMREKGIYF KLVMTQTAGN EIELGNEVGE SKNEIDNLDM
660 670 680 690 700
SSKDSASSLI RRRSTRRSIR GPHDQDRKLS TKEALDEDVP PISFWRILKL
710 720 730 740 750
NSSEWPYFVV GIFCAIVNGA LQPAFSIIFS KVVGVFTRNT DDETKRHDSN
760 770 780 790 800
LFSLLFLILG VISFITFFLQ GFTFGKAGEI LTKRLRYMVF KSMLRQDVSW
810 820 830 840 850
FDNPKNTTGA LTTRLANDAG QVKGATGARL AVITQNIANL GTGIIISLIY
860 870 880 890 900
GWQLTLLLLA IVPIIAIAGV VEMKMLSGQA LKDKKELEGS GKIATEAIEN
910 920 930 940 950
FRTVVSLTRE QKFENMYAQS LQIPYRNALK KAHVFGITFS FTQAMMYFSY
960 970 980 990 1000
AACFRFGAYL VARELMTFEN VLLVFSAIVF GAMAVGQVSS FAPDYAKAKV
1010 1020 1030 1040 1050
SASHIIMIIE KVPSIDSYST GGLKPNTLEG NVKFNEVVFN YPTRPDIPVL
1060 1070 1080 1090 1100
QGLNLEVKKG QTLALVGSSG CGKSTVVQLL ERFYDPMAGT VFLDGKEVNQ
1110 1120 1130 1140 1150
LNVQWLRAHL GIVSQEPILF DCSIAENIAY GDNSRVVSQD EIERAAKEAN
1160 1170 1180 1190 1200
IHQFIESLPD KYNTRVGDKG TQLSGGQKQR IAIARALVRQ PHILLLDEAT
1210 1220 1230 1240 1250
SALDTESEKV VQEALDKARE GRTCIVIAHR LSTIQNADLI VVIQNGKVKE
1260 1270
HGTHQQLLAQ KGIYFSMVSV QAGAKR
Length:1,276
Mass (Da):140,926
Last modified:November 1, 1991 - v2
Checksum:i44F3F92A186B4DFF
GO

Sequence cautioni

The sequence EGW07208 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti338 – 339GA → AP in AAA37004 (PubMed:1671863).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60040 mRNA. Translation: AAA68883.1.
M59253 mRNA. Translation: AAA37004.1.
JH000645 Genomic DNA. Translation: EGW07208.1. Sequence problems.
AH003795 Genomic DNA. Translation: AAP32275.1.
L03286 Genomic DNA. No translation available.
M17897 mRNA. Translation: AAA37006.1.
PIRiA38696. DVHY1C.
RefSeqiNP_001230917.1. NM_001243988.1.

Genome annotation databases

GeneIDi100682536.
KEGGicge:100682536.

Similar proteinsi

Entry informationi

Entry nameiMDR1_CRIGR
AccessioniPrimary (citable) accession number: P21448
Secondary accession number(s): G3HRY0, Q80W58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1991
Last modified: August 30, 2017
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families