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P21448 (MDR1_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug resistance protein 1
EC=3.6.3.44
Alternative name(s):
ATP-binding cassette sub-family B member 1
P-glycoprotein 1
CD_antigen=CD243
Gene names
Name:ABCB1
Synonyms:PGP1, PGY1
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) [Complete proteome]
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length1276 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed at a higher level in intestines than in kidney and liver. Ref.3

Miscellaneous

PGP isoforms differ in their drug transport capabilities: PGP1 and PGP2 can mediate MDR, while PGP3 apparently cannot.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   DomainRepeat
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
Phosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12761276Multidrug resistance protein 1
PRO_0000093339

Regions

Topological domain1 – 5050Cytoplasmic Potential
Transmembrane51 – 7121Helical; Potential
Transmembrane117 – 13721Helical; Potential
Transmembrane186 – 20621Helical; Potential
Transmembrane213 – 23321Helical; Potential
Transmembrane294 – 31421Helical; Potential
Transmembrane323 – 34321Helical; Potential
Topological domain344 – 707364Cytoplasmic Potential
Transmembrane708 – 72821Helical; Potential
Transmembrane754 – 77421Helical; Potential
Transmembrane830 – 85021Helical; Potential
Transmembrane851 – 87121Helical; Potential
Transmembrane934 – 95421Helical; Potential
Transmembrane971 – 99121Helical; Potential
Topological domain992 – 1276285Cytoplasmic Potential
Domain50 – 354305ABC transmembrane type-1 1
Domain389 – 625237ABC transporter 1
Domain708 – 997290ABC transmembrane type-1 2
Domain1032 – 1270239ABC transporter 2
Nucleotide binding424 – 4318ATP 1 Potential
Nucleotide binding1067 – 10748ATP 2 Potential

Amino acid modifications

Modified residue6571Phosphoserine By similarity
Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation8061N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict338 – 3392GA → AP in AAA37004. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P21448 [UniParc].

Last modified November 1, 1991. Version 2.
Checksum: 44F3F92A186B4DFF

FASTA1,276140,926
        10         20         30         40         50         60 
MEFEEDFSGR KDKNFLKMGR KSKKEKKEKK PVVSVFTMFR YAGWLDRLYM LVGTLAAIIH 

        70         80         90        100        110        120 
GVALPLMMLV FGDMTDSFAS VGNIPTNATN NATQVNASDI FGKLEEEMTT YAYYYTGIGA 

       130        140        150        160        170        180 
GVLIVAYIQV SFWCLAAGRQ IHKIRQKFFH AIMNQEIGWF DVHDVGELNT RLTDDVSKIN 

       190        200        210        220        230        240 
EGIGDKIGMF FQAMATFFGG FIIGFTRGWK LTLVILAISP VLGLSAGIWA KILSSFTDKE 

       250        260        270        280        290        300 
LQAYAKAGAV AEEVLAAIRT VIAFGGQKKE LERYNNNLEE AKRLGIKKAI TANISMGAAF 

       310        320        330        340        350        360 
LLIYASYALA FWYGTSLVIS KEYSIGQVLT VFFAVLIGAF SIGQASPNIE AFANARGAAY 

       370        380        390        400        410        420 
EIFNIIDNKP SIDSFSKNGY KPDNIKGNLE FKNIHFSYPS RKDVQILKGL NLKVQSGQTV 

       430        440        450        460        470        480 
ALVGNSGCGK STTVQLLQRL YDPTEGVVSI DGQDIRTINV RYLREIIGVV SQEPVLFATT 

       490        500        510        520        530        540 
IAENIRYGRE NVTMDEIEKA VKEANAYDFI MKLPHKFDTL VGERGAQLSG GQKQRIAIAR 

       550        560        570        580        590        600 
ALVRNPKILL LDEATSALDT ESEAVVQAAL DKAREGRTTI VIAHRLSTVR NADIIAGFDG 

       610        620        630        640        650        660 
GVIVEQGNHE ELMREKGIYF KLVMTQTAGN EIELGNEVGE SKNEIDNLDM SSKDSASSLI 

       670        680        690        700        710        720 
RRRSTRRSIR GPHDQDRKLS TKEALDEDVP PISFWRILKL NSSEWPYFVV GIFCAIVNGA 

       730        740        750        760        770        780 
LQPAFSIIFS KVVGVFTRNT DDETKRHDSN LFSLLFLILG VISFITFFLQ GFTFGKAGEI 

       790        800        810        820        830        840 
LTKRLRYMVF KSMLRQDVSW FDNPKNTTGA LTTRLANDAG QVKGATGARL AVITQNIANL 

       850        860        870        880        890        900 
GTGIIISLIY GWQLTLLLLA IVPIIAIAGV VEMKMLSGQA LKDKKELEGS GKIATEAIEN 

       910        920        930        940        950        960 
FRTVVSLTRE QKFENMYAQS LQIPYRNALK KAHVFGITFS FTQAMMYFSY AACFRFGAYL 

       970        980        990       1000       1010       1020 
VARELMTFEN VLLVFSAIVF GAMAVGQVSS FAPDYAKAKV SASHIIMIIE KVPSIDSYST 

      1030       1040       1050       1060       1070       1080 
GGLKPNTLEG NVKFNEVVFN YPTRPDIPVL QGLNLEVKKG QTLALVGSSG CGKSTVVQLL 

      1090       1100       1110       1120       1130       1140 
ERFYDPMAGT VFLDGKEVNQ LNVQWLRAHL GIVSQEPILF DCSIAENIAY GDNSRVVSQD 

      1150       1160       1170       1180       1190       1200 
EIERAAKEAN IHQFIESLPD KYNTRVGDKG TQLSGGQKQR IAIARALVRQ PHILLLDEAT 

      1210       1220       1230       1240       1250       1260 
SALDTESEKV VQEALDKARE GRTCIVIAHR LSTIQNADLI VVIQNGKVKE HGTHQQLLAQ 

      1270 
KGIYFSMVSV QAGAKR

« Hide

References

[1]"Complete cDNA sequences encoding the Chinese hamster P-glycoprotein gene family."
Endicott J.A., Sarangi F., Ling V.
DNA Seq. 2:89-101(1991) [PubMed: 1685679] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Full length and alternatively spliced pgp1 transcripts in multidrug-resistant Chinese hamster lung cells."
Devine S.E., Hussain A., Davide J.P., Melera P.W.
J. Biol. Chem. 266:4545-4555(1991) [PubMed: 1671863] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Analysis of the Chinese hamster P-glycoprotein/multidrug resistance gene pgp1 reveals that the AP-1 site is essential for full promoter activity."
Teeter L.D., Eckersberg T., Tsai Y., Kuo M.T.
Cell Growth Differ. 2:429-437(1991) [PubMed: 1661134] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21, TISSUE SPECIFICITY.
[4]"Structural and functional analysis of 5' flanking and intron 1 sequences of the hamster P-glycoprotein pgp1 and pgp2 genes."
Zastawny R.L., Ling V.
Biochim. Biophys. Acta 1173:303-313(1993) [PubMed: 8100449] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
Tissue: Ovary.
[5]"Simultaneous expression of two P-glycoprotein genes in drug-sensitive Chinese hamster ovary cells."
Endicott J.A., Juranka P.F., Sarangi F., Gerlach J.H., Deuchars K.L., Ling V.
Mol. Cell. Biol. 7:4075-4081(1987) [PubMed: 2893255] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 706-1276.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M60040 mRNA. Translation: AAA68883.1.
M59253 mRNA. Translation: AAA37004.1.
S81975 Genomic DNA. Translation: AAP32275.1.
L03286 Genomic DNA. No translation available.
M17897 mRNA. Translation: AAA37006.1.
PIRDVHY1C. A38696.
RefSeqNP_001230917.1. NM_001243988.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AP2X-ray2.40P/Q1210-1223[»]
ProteinModelPortalP21448.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100682536.

Phylogenomic databases

HOVERGENHBG080809.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF90123. ABC_TM_1. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDR1_CRIGR
AccessionPrimary (citable) accession number: P21448
Secondary accession number(s): Q80W58
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1991
Last modified: January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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