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Protein

Multidrug resistance protein 1A

Gene

Abcb1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.1 Publication

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi423 – 430ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1066 – 1073ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity, coupled Source: MGI
  • ATPase activity, coupled to transmembrane movement of substances Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • ceramide-translocating ATPase activity Source: BHF-UCL
  • phosphatidylcholine-translocating ATPase activity Source: BHF-UCL
  • phosphatidylethanolamine-translocating ATPase activity Source: BHF-UCL
  • xenobiotic-transporting ATPase activity Source: Reactome

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2161517. Abacavir transmembrane transport.
R-MMU-382556. ABC-family proteins mediated transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein 1A (EC:3.6.3.44)
Alternative name(s):
ATP-binding cassette sub-family B member 1A
MDR1A
Multidrug resistance protein 3
P-glycoprotein 3
Gene namesi
Name:Abcb1a
Synonyms:Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97570. Abcb1a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43CytoplasmicBy similarityAdd BLAST43
Transmembranei44 – 66HelicalAdd BLAST23
Topological domaini67 – 112ExtracellularBy similarityAdd BLAST46
Transmembranei113 – 133HelicalAdd BLAST21
Topological domaini134 – 182CytoplasmicBy similarityAdd BLAST49
Transmembranei183 – 204HelicalAdd BLAST22
Topological domaini205 – 211ExtracellularBy similarity7
Transmembranei212 – 232HelicalAdd BLAST21
Topological domaini233 – 290CytoplasmicBy similarityAdd BLAST58
Transmembranei291 – 312HelicalAdd BLAST22
Topological domaini313 – 326ExtracellularBy similarityAdd BLAST14
Transmembranei327 – 348HelicalAdd BLAST22
Topological domaini349 – 707CytoplasmicBy similarityAdd BLAST359
Transmembranei708 – 728HelicalAdd BLAST21
Topological domaini729 – 752ExtracellularBy similarityAdd BLAST24
Transmembranei753 – 773HelicalAdd BLAST21
Topological domaini774 – 828CytoplasmicBy similarityAdd BLAST55
Transmembranei829 – 849HelicalAdd BLAST21
Topological domaini850ExtracellularBy similarity1
Transmembranei851 – 870HelicalAdd BLAST20
Topological domaini871 – 930CytoplasmicBy similarityAdd BLAST60
Transmembranei931 – 953HelicalAdd BLAST23
Topological domaini954 – 969ExtracellularBy similarityAdd BLAST16
Transmembranei970 – 991HelicalAdd BLAST22
Topological domaini992 – 1276CytoplasmicBy similarityAdd BLAST285

GO - Cellular componenti

  • apical plasma membrane Source: BHF-UCL
  • cell surface Source: MGI
  • extracellular exosome Source: MGI
  • integral component of membrane Source: GO_Central
  • intercellular canaliculus Source: MGI
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2573.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933361 – 1276Multidrug resistance protein 1AAdd BLAST1276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi87N-linked (GlcNAc...)Sequence analysis1
Glycosylationi90N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP21447.
PaxDbiP21447.
PeptideAtlasiP21447.
PRIDEiP21447.

PTM databases

iPTMnetiP21447.
PhosphoSitePlusiP21447.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040584.
GenevisibleiP21447. MM.

Interactioni

Subunit structurei

Interacts with PSMB5.By similarity

Protein-protein interaction databases

IntActiP21447. 1 interactor.
MINTiMINT-4101695.
STRINGi10090.ENSMUSP00000041204.

Chemistry databases

BindingDBiP21447.

Structurei

Secondary structure

11276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni35 – 41Combined sources7
Helixi45 – 86Combined sources42
Turni93 – 97Combined sources5
Helixi98 – 153Combined sources56
Helixi156 – 160Combined sources5
Helixi164 – 181Combined sources18
Helixi184 – 206Combined sources23
Helixi209 – 262Combined sources54
Turni263 – 265Combined sources3
Helixi266 – 318Combined sources53
Helixi324 – 366Combined sources43
Beta strandi388 – 395Combined sources8
Helixi400 – 402Combined sources3
Beta strandi410 – 413Combined sources4
Beta strandi418 – 422Combined sources5
Helixi428 – 435Combined sources8
Turni436 – 438Combined sources3
Beta strandi443 – 453Combined sources11
Turni454 – 456Combined sources3
Helixi459 – 464Combined sources6
Beta strandi466 – 472Combined sources7
Helixi480 – 485Combined sources6
Helixi493 – 502Combined sources10
Helixi506 – 511Combined sources6
Helixi515 – 517Combined sources3
Beta strandi521 – 524Combined sources4
Helixi529 – 542Combined sources14
Beta strandi546 – 552Combined sources7
Beta strandi555 – 557Combined sources3
Helixi559 – 572Combined sources14
Beta strandi574 – 581Combined sources8
Turni585 – 590Combined sources6
Beta strandi592 – 598Combined sources7
Beta strandi601 – 606Combined sources6
Helixi608 – 614Combined sources7
Helixi617 – 625Combined sources9
Helixi695 – 700Combined sources6
Helixi707 – 736Combined sources30
Helixi741 – 743Combined sources3
Helixi744 – 794Combined sources51
Helixi797 – 800Combined sources4
Helixi807 – 825Combined sources19
Helixi827 – 849Combined sources23
Beta strandi850 – 852Combined sources3
Helixi853 – 898Combined sources46
Helixi900 – 906Combined sources7
Helixi909 – 956Combined sources48
Turni957 – 961Combined sources5
Beta strandi962 – 964Combined sources3
Helixi967 – 1009Combined sources43
Beta strandi1031 – 1038Combined sources8
Beta strandi1048 – 1056Combined sources9
Beta strandi1060 – 1069Combined sources10
Helixi1070 – 1079Combined sources10
Beta strandi1086 – 1096Combined sources11
Helixi1097 – 1099Combined sources3
Helixi1102 – 1106Combined sources5
Beta strandi1109 – 1112Combined sources4
Beta strandi1120 – 1122Combined sources3
Helixi1123 – 1127Combined sources5
Helixi1128 – 1130Combined sources3
Helixi1138 – 1147Combined sources10
Helixi1151 – 1156Combined sources6
Beta strandi1157 – 1159Combined sources3
Helixi1160 – 1162Combined sources3
Beta strandi1164 – 1169Combined sources6
Helixi1174 – 1187Combined sources14
Beta strandi1191 – 1195Combined sources5
Helixi1204 – 1217Combined sources14
Beta strandi1218 – 1225Combined sources8
Beta strandi1230 – 1235Combined sources6
Beta strandi1237 – 1243Combined sources7
Beta strandi1246 – 1251Combined sources6
Helixi1253 – 1258Combined sources6
Helixi1262 – 1272Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G5UX-ray3.80A/B1-1276[»]
3G60X-ray4.40A/B1-1276[»]
3G61X-ray4.35A/B1-1276[»]
4KSBX-ray3.80A1-1276[»]
4KSCX-ray4.00A1-1276[»]
4KSDX-ray4.10A1-1276[»]
4LSGX-ray3.80A/B1-1276[»]
4M1MX-ray3.80A/B1-1276[»]
4M2SX-ray4.40A/B1-1276[»]
4M2TX-ray4.35A/B1-1276[»]
4Q9HX-ray3.40A1-1276[»]
4Q9IX-ray3.78A1-1276[»]
4Q9JX-ray3.60A1-1276[»]
4Q9KX-ray3.80A1-1276[»]
4Q9LX-ray3.80A1-1276[»]
4XWKX-ray3.50A1-1276[»]
ProteinModelPortaliP21447.
SMRiP21447.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21447.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 353ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST304
Domaini388 – 624ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini707 – 996ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST290
Domaini1031 – 1269ABC transporter 2PROSITE-ProRule annotationAdd BLAST239

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00530000062896.
HOVERGENiHBG080809.
InParanoidiP21447.
KOiK05658.
OMAiSQGQDRK.
OrthoDBiEOG091G0HVA.
PhylomeDBiP21447.
TreeFamiTF105193.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21447-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELEEDLKGR ADKNFSKMGK KSKKEKKEKK PAVSVLTMFR YAGWLDRLYM
60 70 80 90 100
LVGTLAAIIH GVALPLMMLI FGDMTDSFAS VGNVSKNSTN MSEADKRAMF
110 120 130 140 150
AKLEEEMTTY AYYYTGIGAG VLIVAYIQVS FWCLAAGRQI HKIRQKFFHA
160 170 180 190 200
IMNQEIGWFD VHDVGELNTR LTDDVSKINE GIGDKIGMFF QAMATFFGGF
210 220 230 240 250
IIGFTRGWKL TLVILAISPV LGLSAGIWAK ILSSFTDKEL HAYAKAGAVA
260 270 280 290 300
EEVLAAIRTV IAFGGQKKEL ERYNNNLEEA KRLGIKKAIT ANISMGAAFL
310 320 330 340 350
LIYASYALAF WYGTSLVISK EYSIGQVLTV FFSVLIGAFS VGQASPNIEA
360 370 380 390 400
FANARGAAYE VFKIIDNKPS IDSFSKSGHK PDNIQGNLEF KNIHFSYPSR
410 420 430 440 450
KEVQILKGLN LKVKSGQTVA LVGNSGCGKS TTVQLMQRLY DPLDGMVSID
460 470 480 490 500
GQDIRTINVR YLREIIGVVS QEPVLFATTI AENIRYGRED VTMDEIEKAV
510 520 530 540 550
KEANAYDFIM KLPHQFDTLV GERGAQLSGG QKQRIAIARA LVRNPKILLL
560 570 580 590 600
DEATSALDTE SEAVVQAALD KAREGRTTIV IAHRLSTVRN ADVIAGFDGG
610 620 630 640 650
VIVEQGNHDE LMREKGIYFK LVMTQTAGNE IELGNEACKS KDEIDNLDMS
660 670 680 690 700
SKDSGSSLIR RRSTRKSICG PHDQDRKLST KEALDEDVPP ASFWRILKLN
710 720 730 740 750
STEWPYFVVG IFCAIINGGL QPAFSVIFSK VVGVFTNGGP PETQRQNSNL
760 770 780 790 800
FSLLFLILGI ISFITFFLQG FTFGKAGEIL TKRLRYMVFK SMLRQDVSWF
810 820 830 840 850
DDPKNTTGAL TTRLANDAAQ VKGATGSRLA VIFQNIANLG TGIIISLIYG
860 870 880 890 900
WQLTLLLLAI VPIIAIAGVV EMKMLSGQAL KDKKELEGSG KIATEAIENF
910 920 930 940 950
RTVVSLTREQ KFETMYAQSL QIPYRNAMKK AHVFGITFSF TQAMMYFSYA
960 970 980 990 1000
ACFRFGAYLV TQQLMTFENV LLVFSAIVFG AMAVGQVSSF APDYAKATVS
1010 1020 1030 1040 1050
ASHIIRIIEK TPEIDSYSTQ GLKPNMLEGN VQFSGVVFNY PTRPSIPVLQ
1060 1070 1080 1090 1100
GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE RFYDPMAGSV FLDGKEIKQL
1110 1120 1130 1140 1150
NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVSYEE IVRAAKEANI
1160 1170 1180 1190 1200
HQFIDSLPDK YNTRVGDKGT QLSGGQKQRI AIARALVRQP HILLLDEATS
1210 1220 1230 1240 1250
ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIQNGKVKEH
1260 1270
GTHQQLLAQK GIYFSMVSVQ AGAKRS
Length:1,276
Mass (Da):140,647
Last modified:June 16, 2009 - v3
Checksum:i542E101DF18DDC52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti526 – 527QL → HV in AAA39514 (PubMed:1972547).Curated2
Sequence conflicti526 – 527QL → HV in AAA03243 (PubMed:2473069).Curated2
Sequence conflicti939S → F in AAA39517 (PubMed:1969610).Curated1
Sequence conflicti1036V → F in AAA39517 (PubMed:1969610).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30697 mRNA. Translation: AAA39517.1.
M33581 mRNA. Translation: AAA39514.1.
M33580 Genomic DNA. Translation: AAA39518.1.
AY864315 mRNA. Translation: AAW56448.1.
CH466600 Genomic DNA. Translation: EDL14677.1.
M24417 mRNA. Translation: AAA03243.1.
CCDSiCCDS19084.1.
PIRiA34175. DVMS1A.
A34786.
RefSeqiNP_035206.2. NM_011076.2.
XP_006503618.1. XM_006503555.3.
XP_006503619.1. XM_006503556.3.
UniGeneiMm.207354.

Genome annotation databases

EnsembliENSMUST00000047753; ENSMUSP00000041204; ENSMUSG00000040584.
GeneIDi18671.
KEGGimmu:18671.
UCSCiuc008wkl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30697 mRNA. Translation: AAA39517.1.
M33581 mRNA. Translation: AAA39514.1.
M33580 Genomic DNA. Translation: AAA39518.1.
AY864315 mRNA. Translation: AAW56448.1.
CH466600 Genomic DNA. Translation: EDL14677.1.
M24417 mRNA. Translation: AAA03243.1.
CCDSiCCDS19084.1.
PIRiA34175. DVMS1A.
A34786.
RefSeqiNP_035206.2. NM_011076.2.
XP_006503618.1. XM_006503555.3.
XP_006503619.1. XM_006503556.3.
UniGeneiMm.207354.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G5UX-ray3.80A/B1-1276[»]
3G60X-ray4.40A/B1-1276[»]
3G61X-ray4.35A/B1-1276[»]
4KSBX-ray3.80A1-1276[»]
4KSCX-ray4.00A1-1276[»]
4KSDX-ray4.10A1-1276[»]
4LSGX-ray3.80A/B1-1276[»]
4M1MX-ray3.80A/B1-1276[»]
4M2SX-ray4.40A/B1-1276[»]
4M2TX-ray4.35A/B1-1276[»]
4Q9HX-ray3.40A1-1276[»]
4Q9IX-ray3.78A1-1276[»]
4Q9JX-ray3.60A1-1276[»]
4Q9KX-ray3.80A1-1276[»]
4Q9LX-ray3.80A1-1276[»]
4XWKX-ray3.50A1-1276[»]
ProteinModelPortaliP21447.
SMRiP21447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP21447. 1 interactor.
MINTiMINT-4101695.
STRINGi10090.ENSMUSP00000041204.

Chemistry databases

BindingDBiP21447.
ChEMBLiCHEMBL2573.

PTM databases

iPTMnetiP21447.
PhosphoSitePlusiP21447.

Proteomic databases

MaxQBiP21447.
PaxDbiP21447.
PeptideAtlasiP21447.
PRIDEiP21447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047753; ENSMUSP00000041204; ENSMUSG00000040584.
GeneIDi18671.
KEGGimmu:18671.
UCSCiuc008wkl.1. mouse.

Organism-specific databases

CTDi18671.
MGIiMGI:97570. Abcb1a.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00530000062896.
HOVERGENiHBG080809.
InParanoidiP21447.
KOiK05658.
OMAiSQGQDRK.
OrthoDBiEOG091G0HVA.
PhylomeDBiP21447.
TreeFamiTF105193.

Enzyme and pathway databases

ReactomeiR-MMU-2161517. Abacavir transmembrane transport.
R-MMU-382556. ABC-family proteins mediated transport.

Miscellaneous databases

EvolutionaryTraceiP21447.
PROiP21447.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040584.
GenevisibleiP21447. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDR1A_MOUSE
AccessioniPrimary (citable) accession number: P21447
Secondary accession number(s): Q5I1Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In mouse the MDR gene family includes three or more related but distinct cellular genes.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.