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Protein

Integrase

Gene

int

Organism
Haemophilus phage HP1 (strain HP1c1) (Bacteriophage HP1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei315 – 3151O-(3'-phospho-DNA)-tyrosine intermediate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Integrase (EC:2.7.7.-By similarity, EC:3.1.-.-By similarity)
Gene namesi
Name:int
OrganismiHaemophilus phage HP1 (strain HP1c1) (Bacteriophage HP1)
Taxonomic identifieri1289570 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePeduovirinaeHpunalikevirus
Virus hostiHaemophilus [TaxID: 724]
Proteomesi
  • UP000001713 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337IntegrasePRO_0000197510Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
337
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi176 – 18611Combined sources
Beta strandi189 – 1913Combined sources
Helixi194 – 20411Combined sources
Helixi208 – 2125Combined sources
Turni216 – 2183Combined sources
Beta strandi223 – 23210Combined sources
Beta strandi234 – 2385Combined sources
Helixi241 – 2466Combined sources
Beta strandi251 – 2555Combined sources
Helixi259 – 26810Combined sources
Turni278 – 2803Combined sources
Helixi281 – 29212Combined sources
Helixi297 – 3048Combined sources
Helixi309 – 3124Combined sources
Helixi313 – 3186Combined sources
Helixi325 – 3295Combined sources
Turni331 – 3333Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIHX-ray2.50A/B/C/D168-337[»]
ProteinModelPortaliP21442.
SMRiP21442. Positions 168-337.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21442.

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
[Graphical view]
PfamiPF00589. Phage_integrase. 2 hits.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P21442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRKDTKNG KWLAEVYVNG NASRKWFLTK GDALRFYNQA KEQTTSAVDS
60 70 80 90 100
VQVLESSDLP ALSFYVQEWF DLHGKTLSDG KARLAKLKNL CSNLGDPPAN
110 120 130 140 150
EFNAKIFADY RKRRLDGEFS VNKNNPPKEA TVNREHAYLR AVFNELKSLR
160 170 180 190 200
KWTTENPLDG VRLFKERETE LAFLYERDIY RLLAECDNSR NPDLGLIVRI
210 220 230 240 250
CLATGARWSE AETLTQSQVM PYKITFTNTK SKKNRTVPIS KELFDMLPKK
260 270 280 290 300
RGRLFNDAYE SFENAVLRAE IELPKGQLTH VLRHTFASHF MMNGGNILVL
310 320 330
KEILGHSTIE MTMRYAHFAP SHLESAVKFN PLSNPAQ
Length:337
Mass (Da):38,598
Last modified:May 1, 1991 - v1
Checksum:iD66C28425899D1A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24159 Genomic DNA. Translation: AAB09182.1.
PIRiA33857. RSBPHP.
RefSeqiNP_043466.1. NC_001697.1.

Genome annotation databases

GeneIDi1261146.
KEGGivg:1261146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24159 Genomic DNA. Translation: AAB09182.1.
PIRiA33857. RSBPHP.
RefSeqiNP_043466.1. NC_001697.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIHX-ray2.50A/B/C/D168-337[»]
ProteinModelPortaliP21442.
SMRiP21442. Positions 168-337.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261146.
KEGGivg:1261146.

Miscellaneous databases

EvolutionaryTraceiP21442.

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
[Graphical view]
PfamiPF00589. Phage_integrase. 2 hits.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and expression of the gene for the site-specific integration protein from bacteriophage HP1 of Haemophilus influenzae."
    Goodman S.D., Scocca J.J.
    J. Bacteriol. 171:4232-4240(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of an HP1 phage protein required for site-specific excision."
    Esposito D., Scocca J.J.
    Mol. Microbiol. 13:685-695(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Molecular organization in site-specific recombination: the catalytic domain of bacteriophage HP1 integrase at 2.7-A resolution."
    Hickman A.B., Waninger S., Scocca J.J., Dyda F.
    Cell 89:227-237(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiVINT_BPHC1
AccessioniPrimary (citable) accession number: P21442
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: January 20, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.