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Reviewed, UniProtKB/Swiss-Prot P21441 (MDR_LEITA)

Last modified October 13, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Multidrug resistance protein
    EC=3.6.3.44
Alternative name(s):
    P-glycoprotein
Gene names
Name: PGPA
OrganismLeishmania tarentolae (Sauroleishmania tarentolae)
Taxonomic identifier5689 [NCBI]
Taxonomic lineageEukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmania › lizard Leishmania

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Protein attributes

Sequence length1548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

In this organism, this protein is encoded by a gene, located in "H circle", a 68 kb duplex DNA circle containing a 30 kb inverted repeat. Acquired resistance to methotrexate in the organism is associated with the amplification of h circles with increasing level of LTPGPA gene transcription.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

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Ontologies

Keywords
   Biological processAntibiotic resistance
Transport
   Cellular componentMembrane
   DomainRepeat
Transmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processresponse to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15481548Multidrug resistance protein
PRO_0000093346

Regions

Topological domain1 – 238238Cytoplasmic Potential
Transmembrane239 – 25618 Potential
Transmembrane266 – 28722 Potential
Transmembrane349 – 36719 Potential
Transmembrane375 – 39218 Potential
Transmembrane463 – 48018 Potential
Transmembrane500 – 51920 Potential
Topological domain520 – 932413Cytoplasmic Potential
Transmembrane933 – 95018 Potential
Transmembrane975 – 99319 Potential
Transmembrane1051 – 107020 Potential
Transmembrane1072 – 108817 Potential
Transmembrane1164 – 118219 Potential
Transmembrane1186 – 120520 Potential
Topological domain1206 – 1548343Cytoplasmic Potential
Domain231 – 514284ABC transmembrane type-1 1
Domain634 – 855222ABC transporter 1
Domain940 – 1221282ABC transmembrane type-1 2
Domain1286 – 1521236ABC transporter 2
Nucleotide binding667 – 6748ATP 1 Potential
Nucleotide binding1320 – 13278ATP 2 Potential

Amino acid modifications

Glycosylation2631N-linked (GlcNAc...) Potential
Glycosylation10951N-linked (GlcNAc...) Potential
Glycosylation11541N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
P21441-1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: AFE4CC405C77B2EA

FASTA1,548172,236
        10         20         30         40         50         60 
MVDNGHVTIA MADLGTVVEI AQVRCQQEAQ RKFAEQLDEL WGGEPAYTPT VEDQASWFQQ 

        70         80         90        100        110        120 
LYYGWIGDYI YKAAAGNITE ADLPPPTRST RTYHIGRKLS RQAHADIDAS RRWQGYIGCE 

       130        140        150        160        170        180 
VVYKSEAEAK GVLRWVGHLQ QSDYPRSLVA GVEWRMPPRH RRLAVLGSAA ALHNGVVHGE 

       190        200        210        220        230        240 
RLFWPHEDNY LCSCEPVEQL YVKSKYNLIP PRPPPSPDLL RTLFKVHWYH VWAQILPKLL 

       250        260        270        280        290        300 
SDVTALMLPV LLEYFVKYLN ADNATWGWGL GLALTIFLTN VIQSCSAHKY DHISIRTAAL 

       310        320        330        340        350        360 
FETSSMALLF EKCFTVSRRS LQRPDMSVGR IMNMVGNDVD NIGSLNWYVM YFWSAPLQLV 

       370        380        390        400        410        420 
LCLLLLIRLV GWLRVPGMAV LFVTLPLQAV ISKHVQDVSE RMASVVDLRI KRTNELLSGV 

       430        440        450        460        470        480 
RIVKFMGWEP VFLARIQDAR SRELRCLRDV HVANVFFMFV NDATPTLVIA VVFILYHVSG 

       490        500        510        520        530        540 
KVLKPEVVFP TIALLNTMRV SFFMIPIIIS SILQCFVSAK RVTAFIECPD THSQVQDIAS 

       550        560        570        580        590        600 
IDVPDAAAIF KGASIHTYLP VKLPRCKSRL TAMQRSTLWF RRRGVPETEW YEVDSPDASA 

       610        620        630        640        650        660 
SSLAVHSTTV HMGSTQTVIT DSDGAAGEDE KGEVEEGDRE YYQLVSKELL RNVSLTIPKG 

       670        680        690        700        710        720 
KLTMVIGSTG SGKSTLLGAL MGEYSVESGE LWAERSIAYV PQQAWIMNAT LRGNILFFDE 

       730        740        750        760        770        780 
ERAEDLQDVI RCCQLEADLA QFCGGLDTEI GEMGVNLSGG QKARVSLARA VYANRDVYLL 

       790        800        810        820        830        840 
DDPLSALDAH VGQRIVQDVI LGRLRGKTRV LATHQIHLLP LADYIVVLQH GSIVFAGDFA 

       850        860        870        880        890        900 
AFSATALEET LRGELKGSKD VESCSSDVDT ESATAETAPY VAKAKGLNAE QETSLAGGED 

       910        920        930        940        950        960 
PLRSDVEAGR LMTTEEKATG KVPWSTYVAY LKSCGGLEAW GCLLATFALT ECVTAASSVW 

       970        980        990       1000       1010       1020 
LSIWSTGSLM WSADTYLYVY LFIVFLEIFG SPLRFFLCYY LIRIGSRNMH RDLLESIGVA 

      1030       1040       1050       1060       1070       1080 
RMSFFDTTPV GRVLNRFTKD MSILDNTLND GYLYLLEYFF SMCSTVIIMV VVQPFVLVAI 

      1090       1100       1110       1120       1130       1140 
VPCVYSYYKL MQVYNASNRE TRRIKSIAHS PVFTLLEESL QGQRTIATYG KLHLVLQEAL 

      1150       1160       1170       1180       1190       1200 
GRLDVVYSAL YMQNVSNRWL GVRLEFLSCV VTFMVAFIGV IGKMEGASSQ NIGLISLSLT 

      1210       1220       1230       1240       1250       1260 
MSMTLTETLN WLVRQVAMVE ANMNSVERVL HYTQEVEHEH VPEMGELVAQ LVRSESGRGA 

      1270       1280       1290       1300       1310       1320 
NVTETVVIES AGAASSALHP VQAGSLVLEG VQMRYREGLP LVLRGVSFQI APREKVGIVG 

      1330       1340       1350       1360       1370       1380 
RTGSGKSTLL LTFMRMVEVC GGVIHVNGRE MSAYGLRDVR RHFSMIPQDP VLFDGTVRQN 

      1390       1400       1410       1420       1430       1440 
VDPFLEASSA EVWAALELVG LRERVASESE GIDSRVLEGG SNYSVGQRQL MCMARALLKR 

      1450       1460       1470       1480       1490       1500 
GSGFILMDEA TANIDPALDR QIQATVMSAF SAYTVITIAH RLHTVAQYDK IIVMDHGVVA 

      1510       1520       1530       1540 
EMGSPRELVM NHQSMFHSMV ESLGSRGSKD FYELLMGRRI VQPAVLSD

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References

[1]"The amplified H circle of methotrexate-resistant Leishmania tarentolae contains a novel P-glycoprotein gene."
Ouellette M., Fase-Fowler F., Borst P.
EMBO J. 9:1027-1033(1990) [PubMed: 1969794] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

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Cross-references

Sequence databases

X17154 Genomic DNA. Translation: CAA35038.1.
PIRDVLNS. S09248.

3D structure databases

HSSPHSSP built from PDB template 1NBD based on UniProtKB P13569.
ModBaseSearch...

Protein family/group databases

TCDB3.A.1.208.6. ATP-binding cassette (ABC) superfamily.

Enzyme and pathway databases

BRENDA3.6.3.44. 1138.

Family and domain databases

InterProIPR001140. ABC_TM_transpt.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR003593. ATPase_AAA+_core.
IPR000938. Cytoskel-assoc-prot_CAP-Gly.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
PF01302. CAP_GLY. 1 hit.
[Graphical view]
ProDomPD000006. ABC_transporter. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDR_LEITA
AccessionPrimary (citable) accession number: P21441
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1995
Last modified: October 13, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents