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Reviewed, UniProtKB/Swiss-Prot P21440 (MDR2_MOUSE)

Last modified October 13, 2009. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Multidrug resistance protein 2
    EC=3.6.3.44
Alternative name(s):
    ATP-binding cassette sub-family B member 4
    P-glycoprotein 2
Gene names
Name: Abcb4
Synonyms: Mdr2, Pgy-2, Pgy2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length1276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Mouse MDR2 is not capable of conferring drug resistance.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

In mouse the MDR gene family includes three or more related but distinct cellular genes.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

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Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   DomainRepeat
Transmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

intercellular canaliculus

Inferred from direct assay. Source: MGI

membrane fraction

Inferred from direct assay. Source: MGI

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12761276Multidrug resistance protein 2
PRO_0000093337

Regions

Topological domain1 – 5151Cytoplasmic Potential
Transmembrane52 – 7524 Potential
Transmembrane119 – 13921 Potential
Transmembrane188 – 20821 Potential
Transmembrane215 – 23521 Potential
Transmembrane296 – 31621 Potential
Transmembrane325 – 34521 Potential
Topological domain346 – 707362Cytoplasmic Potential
Transmembrane708 – 72821 Potential
Transmembrane753 – 77321 Potential
Transmembrane829 – 84921 Potential
Transmembrane850 – 87021 Potential
Transmembrane933 – 95321 Potential
Transmembrane970 – 99021 Potential
Topological domain991 – 1276286Cytoplasmic Potential
Domain54 – 356303ABC transmembrane type-1 1
Domain391 – 627237ABC transporter 1
Domain708 – 996289ABC transmembrane type-1 2
Domain1031 – 1269239ABC transporter 2
Nucleotide binding426 – 4338ATP 1 By similarity
Nucleotide binding1066 – 10738ATP 2 By similarity

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation8051N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
P21440-1 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: A6C38DCD2C118EEF

FASTA1,276140,333
        10         20         30         40         50         60 
MDLEAARNGT ARRLDGDFEL GSISNQGREK KKKVNLIGLL TLFRYSDWQD KLFMFLGTLM 

        70         80         90        100        110        120 
AIAHGSGLPL MMIVFGEMTD KFVDNTGNFS LPVNFSLSML NPGRILEEEM TRYAYYYSGL 

       130        140        150        160        170        180 
GGGVLVAAYI QVSFWTLAAG RQIKKIRQKF FHAILRQEMG WFDIKGTTEL NTRLTDDVSK 

       190        200        210        220        230        240 
ISEGIGDKVG MFFQAIATFF AGFIVGFIRG WKLTLVIMAI SPILGLSTAV WAKILSTFSD 

       250        260        270        280        290        300 
KELAAYAKAG AVAEEAPGAI RTVIAFGGQN KELERYQKHL ENAKKIGIKK AISANISMGI 

       310        320        330        340        350        360 
AFLLIYASYA LAFWYGSTLV ISKEYTIGNA MTVFFSILIG AFSVGQAAPC IDAFANARGA 

       370        380        390        400        410        420 
AYVIFDIIDN NPKIDSFSER GHKPDNIKGN LEFSDVHFSY PSRANIKILK GLNLKVKSGQ 

       430        440        450        460        470        480 
TVALVGNSGC GKSTTVQLLQ RLYDPTEGKI SIDGQDIRNF NVRCLREIIG VVSQEPVLFS 

       490        500        510        520        530        540 
TTIAENIRYG RGNVTMDEIE KAVKEANAYD FIMKLPQKFD TLVGDRGAQL SGGQKQRIAI 

       550        560        570        580        590        600 
ARALVRNPKI LLLDEATSAL DTESEAEVQA ALDKAREGRT TIVIAHRLST IRNADVIAGF 

       610        620        630        640        650        660 
EDGVIVEQGS HSELMKKEGI YFRLVNMQTA GSQILSEEFE VELSDEKAAG DVAPNGWKAR 

       670        680        690        700        710        720 
IFRNSTKKSL KSPHQNRLDE ETNELDANVP PVSFLKVLKL NKTEWPYFVV GTVCAIANGA 

       730        740        750        760        770        780 
LQPAFSIILS EMIAIFGPGD DAVKQQKCNM FSLVFLGLGV LSFFTFFLQG FTFGKAGEIL 

       790        800        810        820        830        840 
TTRLRSMAFK AMLRQDMSWF DDHKNSTGAL STRLATDAAQ VQGATGTKLA LIAQNTANLG 

       850        860        870        880        890        900 
TGIIISFIYG WQLTLLLLSV VPFIAVAGIV EMKMLAGNAK RDKKEMEAAG KIATEAIENI 

       910        920        930        940        950        960 
RTVVSLTQER KFESMYVEKL HGPYRNSVRK AHIYGITFSI SQAFMYFSYA GCFRFGSYLI 

       970        980        990       1000       1010       1020 
VNGHMRFKDV ILVFSAIVLG AVALGHASSF APDYAKAKLS AAYLFSLFER QPLIDSYSGE 

      1030       1040       1050       1060       1070       1080 
GLWPDKFEGS VTFNEVVFNY PTRANVPVLQ GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE 

      1090       1100       1110       1120       1130       1140 
RFYDPMAGSV LLDGQEAKKL NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVPHDE 

      1150       1160       1170       1180       1190       1200 
IVRAAKEANI HPFIETLPQK YNTRVGDKGT QLSGGQKQRI AIARALIRQP RVLLLDEATS 

      1210       1220       1230       1240       1250       1260 
ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIENGKVKEH GTHQQLLAQK 

      1270 
GIYFSMVNIQ AGTQNL

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References

[1]"Cloning and characterization of a second member of the mouse mdr gene family."
Gros P., Raymond M., Bell J., Housman D.
Mol. Cell. Biol. 8:2770-2778(1988) [PubMed: 3405218] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"5'-end analysis of the murine mdr2 mRNA reveals complex and tissue-specific processing."
Kirschner L.S., Horwitz S.B.
Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 1-23.
Strain: BALB/c.
[3]"De novo generation of simple sequence during gene amplification."
Kirschner L.S.
Nucleic Acids Res. 24:2829-2834(1996) [PubMed: 8759018] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-92.
Strain: BALB/c.
Tissue: Liver.
+Additional computationally mapped references.

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Cross-references

Sequence databases

J03398 mRNA. Translation: AAA39516.1.
M74151 Genomic DNA. Translation: AAA39515.1.
U46839 Genomic DNA. Translation: AAC52722.1.
IPIIPI00123676.
PIRDVMS2. A30409.
UniGeneMm.297825

3D structure databases

HSSPHSSP built from PDB template 1JJ7 based on UniProtKB Q03518.
ModBaseSearch...

Protein-protein interaction databases

STRINGP21440.

Proteomic databases

PRIDEP21440.

Genome annotation databases

EnsemblENSMUST00000003717; ENSMUSP00000003717; ENSMUSG00000042476; Mus musculus. [Genome view]
UCSCuc008wkr.1. mouse.

Organism-specific databases

MGIMGI:97569. Abcb4.

Phylogenomic databases

HOGENOMP21440.
HOVERGENP21440.

Enzyme and pathway databases

BRENDA3.6.3.44. 244.

Gene expression databases

ArrayExpressP21440.
BgeeP21440.
GenevestigatorP21440.

Family and domain databases

InterProIPR001140. ABC_TM_transpt.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
ProDomPD000006. ABC_transporter. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameMDR2_MOUSE
AccessionPrimary (citable) accession number: P21440
Secondary accession number(s): Q6LCL9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 13, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents