SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P21440

- MDR3_MOUSE

UniProt

P21440 - MDR3_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Multidrug resistance protein 3
Gene
Abcb4, Mdr2, Pgy-2, Pgy2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi426 – 4338ATP 1 By similarity
Nucleotide bindingi1066 – 10738ATP 2 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity, coupled to transmembrane movement of substances Source: RefGenome
  3. xenobiotic-transporting ATPase activity Source: UniProtKB-EC

GO - Biological processi

  1. transmembrane transport Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.44. 244.
ReactomeiREACT_198602. PPARA activates gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein 3 (EC:3.6.3.44)
Alternative name(s):
ATP-binding cassette sub-family B member 4
Multidrug resistance protein 2
P-glycoprotein 2
P-glycoprotein 3
Gene namesi
Name:Abcb4
Synonyms:Mdr2, Pgy-2, Pgy2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:97569. Abcb4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747Cytoplasmic By similarity
Add
BLAST
Transmembranei48 – 7023Helical; By similarity
Add
BLAST
Topological domaini71 – 11545Extracellular By similarity
Add
BLAST
Transmembranei116 – 13621Helical; By similarity
Add
BLAST
Topological domaini137 – 18549Cytoplasmic By similarity
Add
BLAST
Transmembranei186 – 20722Helical; By similarity
Add
BLAST
Topological domaini208 – 2125Extracellular By similarity
Transmembranei213 – 23523Helical; By similarity
Add
BLAST
Topological domaini236 – 29358Cytoplasmic By similarity
Add
BLAST
Transmembranei294 – 31522Helical; By similarity
Add
BLAST
Topological domaini316 – 32914Extracellular By similarity
Add
BLAST
Transmembranei330 – 35122Helical; By similarity
Add
BLAST
Topological domaini352 – 708357Cytoplasmic By similarity
Add
BLAST
Transmembranei709 – 72921Helical; By similarity
Add
BLAST
Topological domaini730 – 75223Extracellular By similarity
Add
BLAST
Transmembranei753 – 77321Helical; By similarity
Add
BLAST
Topological domaini774 – 82855Cytoplasmic By similarity
Add
BLAST
Transmembranei829 – 84921Helical; By similarity
Add
BLAST
Topological domaini850 – 8501Extracellular By similarity
Transmembranei851 – 87020Helical; By similarity
Add
BLAST
Topological domaini871 – 93060Cytoplasmic By similarity
Add
BLAST
Transmembranei931 – 95323Helical; By similarity
Add
BLAST
Topological domaini954 – 96916Extracellular By similarity
Add
BLAST
Transmembranei970 – 99122Helical; By similarity
Add
BLAST
Topological domaini992 – 1276285Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: RefGenome
  2. intercellular canaliculus Source: MGI
  3. membrane Source: MGI
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12761276Multidrug resistance protein 3
PRO_0000093337Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi94 – 941N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP21440.
PaxDbiP21440.
PRIDEiP21440.

PTM databases

PhosphoSiteiP21440.

Expressioni

Gene expression databases

BgeeiP21440.
GenevestigatoriP21440.

Interactioni

Subunit structurei

Interacts with HAX1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP21440.
SMRiP21440. Positions 37-629.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 356303ABC transmembrane type-1 1
Add
BLAST
Domaini391 – 627237ABC transporter 1
Add
BLAST
Domaini708 – 996289ABC transmembrane type-1 2
Add
BLAST
Domaini1031 – 1269239ABC transporter 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni622 – 64625Interaction with HAX1 By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
GeneTreeiENSGT00530000062896.
HOVERGENiHBG080809.
InParanoidiP21440.
KOiK05659.
OMAiTLNEVVF.
OrthoDBiEOG7Z3F4H.
TreeFamiTF105193.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21440-1 [UniParc]FASTAAdd to Basket

« Hide

MDLEAARNGT ARRLDGDFEL GSISNQGREK KKKVNLIGLL TLFRYSDWQD     50
KLFMFLGTLM AIAHGSGLPL MMIVFGEMTD KFVDNTGNFS LPVNFSLSML 100
NPGRILEEEM TRYAYYYSGL GGGVLVAAYI QVSFWTLAAG RQIKKIRQKF 150
FHAILRQEMG WFDIKGTTEL NTRLTDDVSK ISEGIGDKVG MFFQAIATFF 200
AGFIVGFIRG WKLTLVIMAI SPILGLSTAV WAKILSTFSD KELAAYAKAG 250
AVAEEALGAI RTVIAFGGQN KELERYQKHL ENAKKIGIKK AISANISMGI 300
AFLLIYASYA LAFWYGSTLV ISKEYTIGNA MTVFFSILIG AFSVGQAAPC 350
IDAFANARGA AYVIFDIIDN NPKIDSFSER GHKPDNIKGN LEFSDVHFSY 400
PSRANIKILK GLNLKVKSGQ TVALVGNSGC GKSTTVQLLQ RLYDPTEGKI 450
SIDGQDIRNF NVRCLREIIG VVSQEPVLFS TTIAENIRYG RGNVTMDEIE 500
KAVKEANAYD FIMKLPQKFD TLVGDRGAQL SGGQKQRIAI ARALVRNPKI 550
LLLDEATSAL DTESEAEVQA ALDKAREGRT TIVIAHRLST IRNADVIAGF 600
EDGVIVEQGS HSELMKKEGI YFRLVNMQTA GSQILSEEFE VELSDEKAAG 650
DVAPNGWKAR IFRNSTKKSL KSPHQNRLDE ETNELDANVP PVSFLKVLKL 700
NKTEWPYFVV GTVCAIANGA LQPAFSIILS EMIAIFGPGD DAVKQQKCNM 750
FSLVFLGLGV LSFFTFFLQG FTFGKAGEIL TTRLRSMAFK AMLRQDMSWF 800
DDHKNSTGAL STRLATDAAQ VQGATGTRLA LIAQNTANLG TGIIISFIYG 850
WQLTLLLLSV VPFIAVAGIV EMKMLAGNAK RDKKEMEAAG KIATEAIENI 900
RTVVSLTQER KFESMYVEKL HGPYRNSVRK AHIYGITFSI SQAFMYFSYA 950
GCFRFGSYLI VNGHMRFKDV ILVFSAIVLG AVALGHASSF APDYAKAKLS 1000
AAYLFSLFER QPLIDSYSGE GLWPDKFEGS VTFNEVVFNY PTRANVPVLQ 1050
GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE RFYDPMAGSV LLDGQEAKKL 1100
NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVPHDE IVRAAKEANI 1150
HPFIETLPQK YNTRVGDKGT QLSGGQKQRI AIARALIRQP RVLLLDEATS 1200
ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIENGKVKEH 1250
GTHQQLLAQK GIYFSMVNIQ AGTQNL 1276
Length:1,276
Mass (Da):140,377
Last modified:June 28, 2011 - v2
Checksum:i30BC72EDDFA4388C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti257 – 2571L → P in AAA39516. 1 Publication
Sequence conflicti828 – 8281R → K in AAA39516. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03398 mRNA. Translation: AAA39516.1.
CH466600 Genomic DNA. Translation: EDL14681.1.
BC150687 mRNA. Translation: AAI50688.1.
M74151 Genomic DNA. Translation: AAA39515.1.
U46839 Genomic DNA. Translation: AAC52722.1.
CCDSiCCDS19086.1.
PIRiA30409. DVMS2.
RefSeqiNP_032856.2. NM_008830.2.
UniGeneiMm.297825.

Genome annotation databases

EnsembliENSMUST00000003717; ENSMUSP00000003717; ENSMUSG00000042476.
GeneIDi18670.
KEGGimmu:18670.
UCSCiuc008wkr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03398 mRNA. Translation: AAA39516.1 .
CH466600 Genomic DNA. Translation: EDL14681.1 .
BC150687 mRNA. Translation: AAI50688.1 .
M74151 Genomic DNA. Translation: AAA39515.1 .
U46839 Genomic DNA. Translation: AAC52722.1 .
CCDSi CCDS19086.1.
PIRi A30409. DVMS2.
RefSeqi NP_032856.2. NM_008830.2.
UniGenei Mm.297825.

3D structure databases

ProteinModelPortali P21440.
SMRi P21440. Positions 37-629.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P21440.

PTM databases

PhosphoSitei P21440.

Proteomic databases

MaxQBi P21440.
PaxDbi P21440.
PRIDEi P21440.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000003717 ; ENSMUSP00000003717 ; ENSMUSG00000042476 .
GeneIDi 18670.
KEGGi mmu:18670.
UCSCi uc008wkr.2. mouse.

Organism-specific databases

CTDi 5244.
MGIi MGI:97569. Abcb4.

Phylogenomic databases

eggNOGi COG1132.
GeneTreei ENSGT00530000062896.
HOVERGENi HBG080809.
InParanoidi P21440.
KOi K05659.
OMAi TLNEVVF.
OrthoDBi EOG7Z3F4H.
TreeFami TF105193.

Enzyme and pathway databases

BRENDAi 3.6.3.44. 244.
Reactomei REACT_198602. PPARA activates gene expression.

Miscellaneous databases

NextBioi 294682.
PROi P21440.
SOURCEi Search...

Gene expression databases

Bgeei P21440.
Genevestigatori P21440.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEi PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a second member of the mouse mdr gene family."
    Gros P., Raymond M., Bell J., Housman D.
    Mol. Cell. Biol. 8:2770-2778(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "5'-end analysis of the murine mdr2 mRNA reveals complex and tissue-specific processing."
    Kirschner L.S., Horwitz S.B.
    Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
    Strain: BALB/c.
  5. "De novo generation of simple sequence during gene amplification."
    Kirschner L.S.
    Nucleic Acids Res. 24:2829-2834(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-92.
    Strain: BALB/c.
    Tissue: Liver.

Entry informationi

Entry nameiMDR3_MOUSE
AccessioniPrimary (citable) accession number: P21440
Secondary accession number(s): B9EK77, Q6LCL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 28, 2011
Last modified: September 3, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi