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P21440

- MDR3_MOUSE

UniProt

P21440 - MDR3_MOUSE

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Protein

Multidrug resistance protein 3

Gene

Abcb4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi426 – 4338ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1066 – 10738ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  1. ATPase activity, coupled to transmembrane movement of substances Source: RefGenome
  2. ATP binding Source: UniProtKB-KW
  3. xenobiotic-transporting ATPase activity Source: UniProtKB-EC

GO - Biological processi

  1. transmembrane transport Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.44. 244.
ReactomeiREACT_198602. PPARA activates gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein 3 (EC:3.6.3.44)
Alternative name(s):
ATP-binding cassette sub-family B member 4
Multidrug resistance protein 2
P-glycoprotein 2
P-glycoprotein 3
Gene namesi
Name:Abcb4
Synonyms:Mdr2, Pgy-2, Pgy2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:97569. Abcb4.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747CytoplasmicBy similarityAdd
BLAST
Transmembranei48 – 7023HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini71 – 11545ExtracellularBy similarityAdd
BLAST
Transmembranei116 – 13621HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini137 – 18549CytoplasmicBy similarityAdd
BLAST
Transmembranei186 – 20722HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini208 – 2125ExtracellularBy similarity
Transmembranei213 – 23523HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini236 – 29358CytoplasmicBy similarityAdd
BLAST
Transmembranei294 – 31522HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini316 – 32914ExtracellularBy similarityAdd
BLAST
Transmembranei330 – 35122HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini352 – 708357CytoplasmicBy similarityAdd
BLAST
Transmembranei709 – 72921HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini730 – 75223ExtracellularBy similarityAdd
BLAST
Transmembranei753 – 77321HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini774 – 82855CytoplasmicBy similarityAdd
BLAST
Transmembranei829 – 84921HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini850 – 8501ExtracellularBy similarity
Transmembranei851 – 87020HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini871 – 93060CytoplasmicBy similarityAdd
BLAST
Transmembranei931 – 95323HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini954 – 96916ExtracellularBy similarityAdd
BLAST
Transmembranei970 – 99122HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini992 – 1276285CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: RefGenome
  2. intercellular canaliculus Source: MGI
  3. membrane Source: MGI
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12761276Multidrug resistance protein 3PRO_0000093337Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP21440.
PaxDbiP21440.
PRIDEiP21440.

PTM databases

PhosphoSiteiP21440.

Expressioni

Gene expression databases

BgeeiP21440.
GenevestigatoriP21440.

Interactioni

Subunit structurei

Interacts with HAX1.By similarity

Structurei

3D structure databases

ProteinModelPortaliP21440.
SMRiP21440. Positions 37-629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 356303ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini391 – 627237ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini708 – 996289ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1031 – 1269239ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni622 – 64625Interaction with HAX1By similarityAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
GeneTreeiENSGT00530000062896.
HOVERGENiHBG080809.
InParanoidiP21440.
KOiK05659.
OMAiTLNEVVF.
OrthoDBiEOG7Z3F4H.
TreeFamiTF105193.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21440-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLEAARNGT ARRLDGDFEL GSISNQGREK KKKVNLIGLL TLFRYSDWQD
60 70 80 90 100
KLFMFLGTLM AIAHGSGLPL MMIVFGEMTD KFVDNTGNFS LPVNFSLSML
110 120 130 140 150
NPGRILEEEM TRYAYYYSGL GGGVLVAAYI QVSFWTLAAG RQIKKIRQKF
160 170 180 190 200
FHAILRQEMG WFDIKGTTEL NTRLTDDVSK ISEGIGDKVG MFFQAIATFF
210 220 230 240 250
AGFIVGFIRG WKLTLVIMAI SPILGLSTAV WAKILSTFSD KELAAYAKAG
260 270 280 290 300
AVAEEALGAI RTVIAFGGQN KELERYQKHL ENAKKIGIKK AISANISMGI
310 320 330 340 350
AFLLIYASYA LAFWYGSTLV ISKEYTIGNA MTVFFSILIG AFSVGQAAPC
360 370 380 390 400
IDAFANARGA AYVIFDIIDN NPKIDSFSER GHKPDNIKGN LEFSDVHFSY
410 420 430 440 450
PSRANIKILK GLNLKVKSGQ TVALVGNSGC GKSTTVQLLQ RLYDPTEGKI
460 470 480 490 500
SIDGQDIRNF NVRCLREIIG VVSQEPVLFS TTIAENIRYG RGNVTMDEIE
510 520 530 540 550
KAVKEANAYD FIMKLPQKFD TLVGDRGAQL SGGQKQRIAI ARALVRNPKI
560 570 580 590 600
LLLDEATSAL DTESEAEVQA ALDKAREGRT TIVIAHRLST IRNADVIAGF
610 620 630 640 650
EDGVIVEQGS HSELMKKEGI YFRLVNMQTA GSQILSEEFE VELSDEKAAG
660 670 680 690 700
DVAPNGWKAR IFRNSTKKSL KSPHQNRLDE ETNELDANVP PVSFLKVLKL
710 720 730 740 750
NKTEWPYFVV GTVCAIANGA LQPAFSIILS EMIAIFGPGD DAVKQQKCNM
760 770 780 790 800
FSLVFLGLGV LSFFTFFLQG FTFGKAGEIL TTRLRSMAFK AMLRQDMSWF
810 820 830 840 850
DDHKNSTGAL STRLATDAAQ VQGATGTRLA LIAQNTANLG TGIIISFIYG
860 870 880 890 900
WQLTLLLLSV VPFIAVAGIV EMKMLAGNAK RDKKEMEAAG KIATEAIENI
910 920 930 940 950
RTVVSLTQER KFESMYVEKL HGPYRNSVRK AHIYGITFSI SQAFMYFSYA
960 970 980 990 1000
GCFRFGSYLI VNGHMRFKDV ILVFSAIVLG AVALGHASSF APDYAKAKLS
1010 1020 1030 1040 1050
AAYLFSLFER QPLIDSYSGE GLWPDKFEGS VTFNEVVFNY PTRANVPVLQ
1060 1070 1080 1090 1100
GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE RFYDPMAGSV LLDGQEAKKL
1110 1120 1130 1140 1150
NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVPHDE IVRAAKEANI
1160 1170 1180 1190 1200
HPFIETLPQK YNTRVGDKGT QLSGGQKQRI AIARALIRQP RVLLLDEATS
1210 1220 1230 1240 1250
ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIENGKVKEH
1260 1270
GTHQQLLAQK GIYFSMVNIQ AGTQNL
Length:1,276
Mass (Da):140,377
Last modified:June 28, 2011 - v2
Checksum:i30BC72EDDFA4388C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti257 – 2571L → P in AAA39516. (PubMed:3405218)Curated
Sequence conflicti828 – 8281R → K in AAA39516. (PubMed:3405218)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03398 mRNA. Translation: AAA39516.1.
CH466600 Genomic DNA. Translation: EDL14681.1.
BC150687 mRNA. Translation: AAI50688.1.
M74151 Genomic DNA. Translation: AAA39515.1.
U46839 Genomic DNA. Translation: AAC52722.1.
CCDSiCCDS19086.1.
PIRiA30409. DVMS2.
RefSeqiNP_032856.2. NM_008830.2.
UniGeneiMm.297825.

Genome annotation databases

EnsembliENSMUST00000003717; ENSMUSP00000003717; ENSMUSG00000042476.
GeneIDi18670.
KEGGimmu:18670.
UCSCiuc008wkr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03398 mRNA. Translation: AAA39516.1 .
CH466600 Genomic DNA. Translation: EDL14681.1 .
BC150687 mRNA. Translation: AAI50688.1 .
M74151 Genomic DNA. Translation: AAA39515.1 .
U46839 Genomic DNA. Translation: AAC52722.1 .
CCDSi CCDS19086.1.
PIRi A30409. DVMS2.
RefSeqi NP_032856.2. NM_008830.2.
UniGenei Mm.297825.

3D structure databases

ProteinModelPortali P21440.
SMRi P21440. Positions 37-629.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P21440.

Proteomic databases

MaxQBi P21440.
PaxDbi P21440.
PRIDEi P21440.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000003717 ; ENSMUSP00000003717 ; ENSMUSG00000042476 .
GeneIDi 18670.
KEGGi mmu:18670.
UCSCi uc008wkr.2. mouse.

Organism-specific databases

CTDi 5244.
MGIi MGI:97569. Abcb4.

Phylogenomic databases

eggNOGi COG1132.
GeneTreei ENSGT00530000062896.
HOVERGENi HBG080809.
InParanoidi P21440.
KOi K05659.
OMAi TLNEVVF.
OrthoDBi EOG7Z3F4H.
TreeFami TF105193.

Enzyme and pathway databases

BRENDAi 3.6.3.44. 244.
Reactomei REACT_198602. PPARA activates gene expression.

Miscellaneous databases

NextBioi 294682.
PROi P21440.
SOURCEi Search...

Gene expression databases

Bgeei P21440.
Genevestigatori P21440.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEi PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a second member of the mouse mdr gene family."
    Gros P., Raymond M., Bell J., Housman D.
    Mol. Cell. Biol. 8:2770-2778(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "5'-end analysis of the murine mdr2 mRNA reveals complex and tissue-specific processing."
    Kirschner L.S., Horwitz S.B.
    Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
    Strain: BALB/c.
  5. "De novo generation of simple sequence during gene amplification."
    Kirschner L.S.
    Nucleic Acids Res. 24:2829-2834(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-92.
    Strain: BALB/c.
    Tissue: Liver.

Entry informationi

Entry nameiMDR3_MOUSE
AccessioniPrimary (citable) accession number: P21440
Secondary accession number(s): B9EK77, Q6LCL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 28, 2011
Last modified: November 26, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3