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P21440 (MDR3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug resistance protein 3

EC=3.6.3.44
Alternative name(s):
ATP-binding cassette sub-family B member 4
Multidrug resistance protein 2
P-glycoprotein 2
P-glycoprotein 3
Gene names
Name:Abcb4
Synonyms:Mdr2, Pgy-2, Pgy2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1276 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subunit structure

Interacts with HAX1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12761276Multidrug resistance protein 3
PRO_0000093337

Regions

Topological domain1 – 4747Cytoplasmic By similarity
Transmembrane48 – 7023Helical; By similarity
Topological domain71 – 11545Extracellular By similarity
Transmembrane116 – 13621Helical; By similarity
Topological domain137 – 18549Cytoplasmic By similarity
Transmembrane186 – 20722Helical; By similarity
Topological domain208 – 2125Extracellular By similarity
Transmembrane213 – 23523Helical; By similarity
Topological domain236 – 29358Cytoplasmic By similarity
Transmembrane294 – 31522Helical; By similarity
Topological domain316 – 32914Extracellular By similarity
Transmembrane330 – 35122Helical; By similarity
Topological domain352 – 708357Cytoplasmic By similarity
Transmembrane709 – 72921Helical; By similarity
Topological domain730 – 75223Extracellular By similarity
Transmembrane753 – 77321Helical; By similarity
Topological domain774 – 82855Cytoplasmic By similarity
Transmembrane829 – 84921Helical; By similarity
Topological domain8501Extracellular By similarity
Transmembrane851 – 87020Helical; By similarity
Topological domain871 – 93060Cytoplasmic By similarity
Transmembrane931 – 95323Helical; By similarity
Topological domain954 – 96916Extracellular By similarity
Transmembrane970 – 99122Helical; By similarity
Topological domain992 – 1276285Cytoplasmic By similarity
Domain54 – 356303ABC transmembrane type-1 1
Domain391 – 627237ABC transporter 1
Domain708 – 996289ABC transmembrane type-1 2
Domain1031 – 1269239ABC transporter 2
Nucleotide binding426 – 4338ATP 1 By similarity
Nucleotide binding1066 – 10738ATP 2 By similarity
Region622 – 64625Interaction with HAX1 By similarity

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation941N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2571L → P in AAA39516. Ref.1
Sequence conflict8281R → K in AAA39516. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P21440 [UniParc].

Last modified June 28, 2011. Version 2.
Checksum: 30BC72EDDFA4388C

FASTA1,276140,377
        10         20         30         40         50         60 
MDLEAARNGT ARRLDGDFEL GSISNQGREK KKKVNLIGLL TLFRYSDWQD KLFMFLGTLM 

        70         80         90        100        110        120 
AIAHGSGLPL MMIVFGEMTD KFVDNTGNFS LPVNFSLSML NPGRILEEEM TRYAYYYSGL 

       130        140        150        160        170        180 
GGGVLVAAYI QVSFWTLAAG RQIKKIRQKF FHAILRQEMG WFDIKGTTEL NTRLTDDVSK 

       190        200        210        220        230        240 
ISEGIGDKVG MFFQAIATFF AGFIVGFIRG WKLTLVIMAI SPILGLSTAV WAKILSTFSD 

       250        260        270        280        290        300 
KELAAYAKAG AVAEEALGAI RTVIAFGGQN KELERYQKHL ENAKKIGIKK AISANISMGI 

       310        320        330        340        350        360 
AFLLIYASYA LAFWYGSTLV ISKEYTIGNA MTVFFSILIG AFSVGQAAPC IDAFANARGA 

       370        380        390        400        410        420 
AYVIFDIIDN NPKIDSFSER GHKPDNIKGN LEFSDVHFSY PSRANIKILK GLNLKVKSGQ 

       430        440        450        460        470        480 
TVALVGNSGC GKSTTVQLLQ RLYDPTEGKI SIDGQDIRNF NVRCLREIIG VVSQEPVLFS 

       490        500        510        520        530        540 
TTIAENIRYG RGNVTMDEIE KAVKEANAYD FIMKLPQKFD TLVGDRGAQL SGGQKQRIAI 

       550        560        570        580        590        600 
ARALVRNPKI LLLDEATSAL DTESEAEVQA ALDKAREGRT TIVIAHRLST IRNADVIAGF 

       610        620        630        640        650        660 
EDGVIVEQGS HSELMKKEGI YFRLVNMQTA GSQILSEEFE VELSDEKAAG DVAPNGWKAR 

       670        680        690        700        710        720 
IFRNSTKKSL KSPHQNRLDE ETNELDANVP PVSFLKVLKL NKTEWPYFVV GTVCAIANGA 

       730        740        750        760        770        780 
LQPAFSIILS EMIAIFGPGD DAVKQQKCNM FSLVFLGLGV LSFFTFFLQG FTFGKAGEIL 

       790        800        810        820        830        840 
TTRLRSMAFK AMLRQDMSWF DDHKNSTGAL STRLATDAAQ VQGATGTRLA LIAQNTANLG 

       850        860        870        880        890        900 
TGIIISFIYG WQLTLLLLSV VPFIAVAGIV EMKMLAGNAK RDKKEMEAAG KIATEAIENI 

       910        920        930        940        950        960 
RTVVSLTQER KFESMYVEKL HGPYRNSVRK AHIYGITFSI SQAFMYFSYA GCFRFGSYLI 

       970        980        990       1000       1010       1020 
VNGHMRFKDV ILVFSAIVLG AVALGHASSF APDYAKAKLS AAYLFSLFER QPLIDSYSGE 

      1030       1040       1050       1060       1070       1080 
GLWPDKFEGS VTFNEVVFNY PTRANVPVLQ GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE 

      1090       1100       1110       1120       1130       1140 
RFYDPMAGSV LLDGQEAKKL NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVPHDE 

      1150       1160       1170       1180       1190       1200 
IVRAAKEANI HPFIETLPQK YNTRVGDKGT QLSGGQKQRI AIARALIRQP RVLLLDEATS 

      1210       1220       1230       1240       1250       1260 
ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIENGKVKEH GTHQQLLAQK 

      1270 
GIYFSMVNIQ AGTQNL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a second member of the mouse mdr gene family."
Gros P., Raymond M., Bell J., Housman D.
Mol. Cell. Biol. 8:2770-2778(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"5'-end analysis of the murine mdr2 mRNA reveals complex and tissue-specific processing."
Kirschner L.S., Horwitz S.B.
Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
Strain: BALB/c.
[5]"De novo generation of simple sequence during gene amplification."
Kirschner L.S.
Nucleic Acids Res. 24:2829-2834(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-92.
Strain: BALB/c.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03398 mRNA. Translation: AAA39516.1.
CH466600 Genomic DNA. Translation: EDL14681.1.
BC150687 mRNA. Translation: AAI50688.1.
M74151 Genomic DNA. Translation: AAA39515.1.
U46839 Genomic DNA. Translation: AAC52722.1.
PIRDVMS2. A30409.
RefSeqNP_032856.2. NM_008830.2.
UniGeneMm.297825.

3D structure databases

ProteinModelPortalP21440.
SMRP21440. Positions 37-629.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP21440.

PTM databases

PhosphoSiteP21440.

Proteomic databases

PaxDbP21440.
PRIDEP21440.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003717; ENSMUSP00000003717; ENSMUSG00000042476.
GeneID18670.
KEGGmmu:18670.
UCSCuc008wkr.2. mouse.

Organism-specific databases

CTD5244.
MGIMGI:97569. Abcb4.

Phylogenomic databases

eggNOGCOG1132.
GeneTreeENSGT00530000062896.
HOVERGENHBG080809.
InParanoidP21440.
KOK05659.
OMAFATIDEQ.
OrthoDBEOG7Z3F4H.
TreeFamTF105193.

Enzyme and pathway databases

BRENDA3.6.3.44. 244.

Gene expression databases

BgeeP21440.
GenevestigatorP21440.

Family and domain databases

InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio294682.
PROP21440.
SOURCESearch...

Entry information

Entry nameMDR3_MOUSE
AccessionPrimary (citable) accession number: P21440
Secondary accession number(s): B9EK77, Q6LCL9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 28, 2011
Last modified: March 19, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot