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P21440

- MDR3_MOUSE

UniProt

P21440 - MDR3_MOUSE

Protein

Multidrug resistance protein 3

Gene

Abcb4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

    Catalytic activityi

    ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi426 – 4338ATP 1PROSITE-ProRule annotation
    Nucleotide bindingi1066 – 10738ATP 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATPase activity, coupled to transmembrane movement of substances Source: RefGenome
    2. ATP binding Source: UniProtKB-KW
    3. xenobiotic-transporting ATPase activity Source: UniProtKB-EC

    GO - Biological processi

    1. transmembrane transport Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi3.6.3.44. 244.
    ReactomeiREACT_198602. PPARA activates gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multidrug resistance protein 3 (EC:3.6.3.44)
    Alternative name(s):
    ATP-binding cassette sub-family B member 4
    Multidrug resistance protein 2
    P-glycoprotein 2
    P-glycoprotein 3
    Gene namesi
    Name:Abcb4
    Synonyms:Mdr2, Pgy-2, Pgy2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:97569. Abcb4.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein PROSITE-ProRule annotation

    GO - Cellular componenti

    1. integral component of membrane Source: RefGenome
    2. intercellular canaliculus Source: MGI
    3. membrane Source: MGI
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12761276Multidrug resistance protein 3PRO_0000093337Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP21440.
    PaxDbiP21440.
    PRIDEiP21440.

    PTM databases

    PhosphoSiteiP21440.

    Expressioni

    Gene expression databases

    BgeeiP21440.
    GenevestigatoriP21440.

    Interactioni

    Subunit structurei

    Interacts with HAX1.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP21440.
    SMRiP21440. Positions 37-629.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4747CytoplasmicBy similarityAdd
    BLAST
    Topological domaini71 – 11545ExtracellularBy similarityAdd
    BLAST
    Topological domaini137 – 18549CytoplasmicBy similarityAdd
    BLAST
    Topological domaini208 – 2125ExtracellularBy similarity
    Topological domaini236 – 29358CytoplasmicBy similarityAdd
    BLAST
    Topological domaini316 – 32914ExtracellularBy similarityAdd
    BLAST
    Topological domaini352 – 708357CytoplasmicBy similarityAdd
    BLAST
    Topological domaini730 – 75223ExtracellularBy similarityAdd
    BLAST
    Topological domaini774 – 82855CytoplasmicBy similarityAdd
    BLAST
    Topological domaini850 – 8501ExtracellularBy similarity
    Topological domaini871 – 93060CytoplasmicBy similarityAdd
    BLAST
    Topological domaini954 – 96916ExtracellularBy similarityAdd
    BLAST
    Topological domaini992 – 1276285CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei48 – 7023HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei116 – 13621HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei186 – 20722HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei213 – 23523HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei294 – 31522HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei330 – 35122HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei709 – 72921HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei753 – 77321HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei829 – 84921HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei851 – 87020HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei931 – 95323HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei970 – 99122HelicalPROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 356303ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini391 – 627237ABC transporter 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini708 – 996289ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1031 – 1269239ABC transporter 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni622 – 64625Interaction with HAX1By similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
    Contains 2 ABC transporter domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1132.
    GeneTreeiENSGT00530000062896.
    HOVERGENiHBG080809.
    InParanoidiP21440.
    KOiK05659.
    OMAiTLNEVVF.
    OrthoDBiEOG7Z3F4H.
    TreeFamiTF105193.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 2 hits.
    PROSITEiPS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 2 hits.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21440-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLEAARNGT ARRLDGDFEL GSISNQGREK KKKVNLIGLL TLFRYSDWQD     50
    KLFMFLGTLM AIAHGSGLPL MMIVFGEMTD KFVDNTGNFS LPVNFSLSML 100
    NPGRILEEEM TRYAYYYSGL GGGVLVAAYI QVSFWTLAAG RQIKKIRQKF 150
    FHAILRQEMG WFDIKGTTEL NTRLTDDVSK ISEGIGDKVG MFFQAIATFF 200
    AGFIVGFIRG WKLTLVIMAI SPILGLSTAV WAKILSTFSD KELAAYAKAG 250
    AVAEEALGAI RTVIAFGGQN KELERYQKHL ENAKKIGIKK AISANISMGI 300
    AFLLIYASYA LAFWYGSTLV ISKEYTIGNA MTVFFSILIG AFSVGQAAPC 350
    IDAFANARGA AYVIFDIIDN NPKIDSFSER GHKPDNIKGN LEFSDVHFSY 400
    PSRANIKILK GLNLKVKSGQ TVALVGNSGC GKSTTVQLLQ RLYDPTEGKI 450
    SIDGQDIRNF NVRCLREIIG VVSQEPVLFS TTIAENIRYG RGNVTMDEIE 500
    KAVKEANAYD FIMKLPQKFD TLVGDRGAQL SGGQKQRIAI ARALVRNPKI 550
    LLLDEATSAL DTESEAEVQA ALDKAREGRT TIVIAHRLST IRNADVIAGF 600
    EDGVIVEQGS HSELMKKEGI YFRLVNMQTA GSQILSEEFE VELSDEKAAG 650
    DVAPNGWKAR IFRNSTKKSL KSPHQNRLDE ETNELDANVP PVSFLKVLKL 700
    NKTEWPYFVV GTVCAIANGA LQPAFSIILS EMIAIFGPGD DAVKQQKCNM 750
    FSLVFLGLGV LSFFTFFLQG FTFGKAGEIL TTRLRSMAFK AMLRQDMSWF 800
    DDHKNSTGAL STRLATDAAQ VQGATGTRLA LIAQNTANLG TGIIISFIYG 850
    WQLTLLLLSV VPFIAVAGIV EMKMLAGNAK RDKKEMEAAG KIATEAIENI 900
    RTVVSLTQER KFESMYVEKL HGPYRNSVRK AHIYGITFSI SQAFMYFSYA 950
    GCFRFGSYLI VNGHMRFKDV ILVFSAIVLG AVALGHASSF APDYAKAKLS 1000
    AAYLFSLFER QPLIDSYSGE GLWPDKFEGS VTFNEVVFNY PTRANVPVLQ 1050
    GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE RFYDPMAGSV LLDGQEAKKL 1100
    NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVPHDE IVRAAKEANI 1150
    HPFIETLPQK YNTRVGDKGT QLSGGQKQRI AIARALIRQP RVLLLDEATS 1200
    ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIENGKVKEH 1250
    GTHQQLLAQK GIYFSMVNIQ AGTQNL 1276
    Length:1,276
    Mass (Da):140,377
    Last modified:June 28, 2011 - v2
    Checksum:i30BC72EDDFA4388C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti257 – 2571L → P in AAA39516. (PubMed:3405218)Curated
    Sequence conflicti828 – 8281R → K in AAA39516. (PubMed:3405218)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03398 mRNA. Translation: AAA39516.1.
    CH466600 Genomic DNA. Translation: EDL14681.1.
    BC150687 mRNA. Translation: AAI50688.1.
    M74151 Genomic DNA. Translation: AAA39515.1.
    U46839 Genomic DNA. Translation: AAC52722.1.
    CCDSiCCDS19086.1.
    PIRiA30409. DVMS2.
    RefSeqiNP_032856.2. NM_008830.2.
    UniGeneiMm.297825.

    Genome annotation databases

    EnsembliENSMUST00000003717; ENSMUSP00000003717; ENSMUSG00000042476.
    GeneIDi18670.
    KEGGimmu:18670.
    UCSCiuc008wkr.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03398 mRNA. Translation: AAA39516.1 .
    CH466600 Genomic DNA. Translation: EDL14681.1 .
    BC150687 mRNA. Translation: AAI50688.1 .
    M74151 Genomic DNA. Translation: AAA39515.1 .
    U46839 Genomic DNA. Translation: AAC52722.1 .
    CCDSi CCDS19086.1.
    PIRi A30409. DVMS2.
    RefSeqi NP_032856.2. NM_008830.2.
    UniGenei Mm.297825.

    3D structure databases

    ProteinModelPortali P21440.
    SMRi P21440. Positions 37-629.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P21440.

    PTM databases

    PhosphoSitei P21440.

    Proteomic databases

    MaxQBi P21440.
    PaxDbi P21440.
    PRIDEi P21440.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003717 ; ENSMUSP00000003717 ; ENSMUSG00000042476 .
    GeneIDi 18670.
    KEGGi mmu:18670.
    UCSCi uc008wkr.2. mouse.

    Organism-specific databases

    CTDi 5244.
    MGIi MGI:97569. Abcb4.

    Phylogenomic databases

    eggNOGi COG1132.
    GeneTreei ENSGT00530000062896.
    HOVERGENi HBG080809.
    InParanoidi P21440.
    KOi K05659.
    OMAi TLNEVVF.
    OrthoDBi EOG7Z3F4H.
    TreeFami TF105193.

    Enzyme and pathway databases

    BRENDAi 3.6.3.44. 244.
    Reactomei REACT_198602. PPARA activates gene expression.

    Miscellaneous databases

    NextBioi 294682.
    PROi P21440.
    SOURCEi Search...

    Gene expression databases

    Bgeei P21440.
    Genevestigatori P21440.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 2 hits.
    PROSITEi PS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 2 hits.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a second member of the mouse mdr gene family."
      Gros P., Raymond M., Bell J., Housman D.
      Mol. Cell. Biol. 8:2770-2778(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "5'-end analysis of the murine mdr2 mRNA reveals complex and tissue-specific processing."
      Kirschner L.S., Horwitz S.B.
      Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
      Strain: BALB/c.
    5. "De novo generation of simple sequence during gene amplification."
      Kirschner L.S.
      Nucleic Acids Res. 24:2829-2834(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-92.
      Strain: BALB/c.
      Tissue: Liver.

    Entry informationi

    Entry nameiMDR3_MOUSE
    AccessioniPrimary (citable) accession number: P21440
    Secondary accession number(s): B9EK77, Q6LCL9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3