P21411 (GAG_SMRVH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gag polyprotein

Cleaved into the following 4 chains:

1. Core protein p19
2. Core protein p16
3. Putative nucleocapsid protein p35
4. Probable core protein p10

Gene names

Name:

gag

Organism

Squirrel monkey retrovirus (SMRV-H) (SMRV-HLB) [Complete proteome]

Taxonomic identifier

11856 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Betaretrovirus

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Putative nucleocapsid protein p35: Virion Potential.
Domain	Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p35 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 Potential.

Ontologies

Keywords
Biological process	Host-virus interaction Viral budding Viral budding via the host ESCRT complexes Virus exit from host cell
Cellular component	Virion
Ligand	Viral nucleoprotein
Molecular function	Capsid protein
PTM	Lipoprotein Myristate
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	viral release from host cell Inferred from electronic annotation. Source: UniProtKB-KW virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host By similarity
	Chain	2 – 163	162	Core protein p19		PRO_0000040956
	Chain	164 – 318	155	Core protein p16		PRO_0000040957
	Chain	319 – 648	330	Putative nucleocapsid protein p35		PRO_0000040958
	Chain	649 – 740	92	Probable core protein p10		PRO_0000040959
Regions
	Motif	396 – 399	4	PTAP/PSAP motif Potential
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine; by host By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21411 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: CC2503C2661221F5	FASTA	740	80,543
	10 20 30 40 50 60 MGQASSHSEN DLFISHLKES LKVRRIRVRK KDLVSFFSFI FKTCPWFPQE GSIDSRVWGR 70 80 90 100 110 120 VGDCLNDYYR VFGPETIPIT TFNYYNLIRD VLTNQSDSPD IQRLCKEGHK ILISHSRPPS 130 140 150 160 170 180 RQAPVTITTS EKASSRPPSR APSTCPSVAI DIGSHDTGQS SLYPNLATLT DPPIQSPHSR 190 200 210 220 230 240 AHTPPQHLPL LANSKTLHNS GSQDDQLNPA DQADLEEAAA QYNNPDWPQL TNTPALPPFR 250 260 270 280 290 300 PPSYVSTAVP PVAVAAPVLH APTSGVPGSP TAPNLPGVAL AKPSGPIDET VSLLDGVKTL 310 320 330 340 350 360 VTKLSDLALL PPAGVMAFPV TRSQGQVSSN TTGRASPHPD THTIPEEEEA DSGESDSEDD 370 380 390 400 410 420 EEESSEPTEP TYTHSYKRLN LKTIEKIKTA VANYGPTAPF TVALVESLSE RWLTPSDWFF 430 440 450 460 470 480 LSRAALSGGD NILWKSEYED ISKQFAERTR VRPPPKDGPL KIPGASPYQN NDKQAQFPPG 490 500 510 520 530 540 LLTQIQSAGL KAWKRLPQKG AATTSLAKIR QGPDESYSDF VSRLQETADR LFGSGESESS 550 560 570 580 590 600 FVKHLAYENA NPACQSAIRP FRQKELSTMS PLLWYCSAHA VGLAIGAALQ NLAPAQLLEP 610 620 630 640 650 660 RPAFAIIVTN PAIFQETAPK KIQPPTQLPT QPNAPQASLI KNLGPTTKCP RCKKGFHWAS 670 680 690 700 710 720 ECRSRLDING QPIIKQGNLN RGQPQGPTTG MNSGASQFTP QYRQPTPALP VINHAATSQT 730 740 SGEQQRAVQD WTSVPPPTQY

References

[1]

"Molecular cloning, complete nucleotide sequence, and gene structure of the provirus genome of a retrovirus produced in a human lymphoblastoid cell line." Oda T., Ikeda S., Watanabe S., Hatsushika M., Akiyama K., Mitsunobu F. Virology 167:468-476(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23385 Genomic RNA. Translation: AAA66451.1.
PIR	FOLJHD. A31827.
RefSeq	NP_041259.1. NC_001514.1.
3D structure databases
ProteinModelPortal	P21411.
SMR	P21411. Positions 648-674.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1491964.
Family and domain databases
Gene3D	1.10.1200.30. 1 hit. 1.10.375.10. 1 hit. 4.10.60.10. 1 hit.
InterPro	IPR003322. B_retro_matrix_N. IPR000721. Gag_p24. IPR008916. Retrov_capsid_C. IPR008919. Retrov_capsid_N. IPR010999. Retrovr_matrix_N. IPR001878. Znf_CCHC. [Graphical view]
Pfam	PF02337. Gag_p10. 1 hit. PF00607. Gag_p24. 1 hit. [Graphical view]
ProDom	PD004265. B_retro_matrix_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00343. ZnF_C2HC. 1 hit. [Graphical view]
SUPFAM	SSF47353. Retrov_capsid_C. 1 hit. SSF47943. Retrov_capsid_N. 1 hit. SSF47836. Retrovir_matrix. 1 hit. SSF57756. SSF57756. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GAG_SMRVH

Accession

Primary (citable) accession number: P21411

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program