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Protein

Envelopment polyprotein

Gene

GP

Organism
Rift valley fever virus (strain ZH-548 M12) (RVFV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycoprotein N: Structural component of the virion that interacts with glycoprotein N. About 720 Gn and 720 Gc proteins form 12 pentameric and 110 hexameric capsomeres. Theses capsomeres are arranged on the virus envelop surface in an icosahedral lattice with a T=12 quasisymmetry. Attaches the virion to a cell receptor and thereby promotes fusion after endocytosis of the virion. Contains a Golgi retention signal on its C-terminal region and brings Gc to the host Golgi apparatus where assembly occurs.2 Publications
Glycoprotein C: Structural component of the virion that interacts with glycoprotein C. About 720 Gn and 720 Gc proteins form 12 pentameric and 110 hexameric capsomeres. Theses capsomeres are arranged on the virus envelop surface in an icosahedral lattice with a T=12 quasisymmetry. Attaches the virion to a cell receptor and thereby promotes fusion after endocytosis of the virion.1 Publication
Isoform NSm protein: Plays a role in the inhibition of virus-induced apoptosis. Plays a role for virus dissemination in vertebrates.2 Publications
NSm-Gn protein: Plays a role for virus dissemination in mosquitoes. May act as a strucutral virion protein in insects.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Modulation of host cell apoptosis by virus, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelopment polyprotein
Alternative name(s):
M polyprotein
Cleaved into the following 3 chains:
NSm-Gn protein1 Publication
Alternative name(s):
p78 protein
Glycoprotein N1 Publication
Short name:
Gn
Alternative name(s):
Glycoprotein G1
Glycoprotein C1 Publication
Short name:
Gc
Alternative name(s):
Glycoprotein G2
Gene namesi
Name:GP
OrganismiRift valley fever virus (strain ZH-548 M12) (RVFV)
Taxonomic identifieri11589 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesBunyaviridaePhlebovirus
Virus hostiAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Bos taurus x Bison bison (beefalo) [TaxID: 297284]
Camelus bactrianus (Bactrian camel) [TaxID: 9837]
Capra hircus (Goat) [TaxID: 9925]
Homo sapiens (Human) [TaxID: 9606]
Ovis aries (Sheep) [TaxID: 9940]
Phlebotomus papatasi (Sandfly) [TaxID: 29031]
Proteomesi
  • UP000002477 Componenti: Genome

Subcellular locationi

Glycoprotein N :
Glycoprotein C :
Isoform NSm protein :
  • Host mitochondrion outer membrane 1 Publication
NSm-Gn protein :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 582566LumenalSequence analysisAdd
BLAST
Transmembranei583 – 60321HelicalSequence analysisAdd
BLAST
Topological domaini604 – 69087CytoplasmicSequence analysisAdd
BLAST
Topological domaini691 – 1159469LumenalSequence analysisAdd
BLAST
Transmembranei1160 – 118021HelicalSequence analysisAdd
BLAST
Topological domaini1181 – 119717CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • host cell Golgi membrane Source: UniProtKB
  • host cell mitochondrial outer membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • virion Source: UniProtKB
  • virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Host mitochondrion, Host mitochondrion outer membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence analysisAdd
BLAST
Chaini17 – 11971181Envelopment polyproteinPRO_0000247010Add
BLAST
Chaini17 – 690674NSm-Gn proteinPRO_0000434914Add
BLAST
Chaini154 – 690537Glycoprotein NSequence analysisPRO_0000036851Add
BLAST
Chaini691 – 1197507Glycoprotein CSequence analysisPRO_0000036852Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi438 – 4381N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi794 – 7941N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi1035 – 10351N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi1077 – 10771N-linked (GlcNAc...); by hostSequence analysis

Post-translational modificationi

Envelopment polyprotein: Specific enzymatic cleavages in vivo yield mature proteins including NSm protein, Glycoprotein C, and Glycoprotein N.1 Publication
Glycoprotein C: Glycosylated by host (PubMed:2728348). The glycans can attach to host CD209/DC-SIGN, and may play a role in virus entry into dendritic cells (PubMed:21767814).2 Publications
Glycoprotein N: Glycosylated by host (PubMed:2728348). The glycans can attach to host CD209/DC-SIGN, and may play a role in virus entry into dendritic cells (PubMed:21767814).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei690 – 6912Cleavage; by host signal peptidaseBy similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP21401.

Interactioni

Subunit structurei

Glycoprotein C and Glycoprotein N interact with each other. Glycoprotein Gn interacts with nucleocapsid protein N and with the polymerase L in order to package them into virus particles.1 Publication

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR016404. M_polyprot_prcur_phlebovir.
IPR010826. Phlebovirus_G1.
IPR009878. Phlebovirus_G2.
IPR009879. Phlebovirus_NSM.
[Graphical view]
PfamiPF07243. Phlebovirus_G1. 1 hit.
PF07245. Phlebovirus_G2. 1 hit.
PF07246. Phlebovirus_NSM. 2 hits.
[Graphical view]
PIRSFiPIRSF003961. M_poly_PhleboV. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Envelopment polyprotein (identifier: P21401-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYVLLTILTS VLVCEAIIRV SLSSTREETC FGDSTNPEMI EGAWDSLREE
60 70 80 90 100
EMPEELSCSI SGIREVKTSS QELYRALKAI IAADGLNNIT CHGKDPEDKI
110 120 130 140 150
SLIKGPPHKK RVGIVRCERR RDAKQIGRKT MAGIAMTVLP ALAVFALAPV
160 170 180 190 200
VFAEDPHLRN RPGKGHNYID GMTQEDATCK PVTYAGACSS FDVLLEKGKF
210 220 230 240 250
PLFQSYAHHR TLLEAVHDTI IAKADPPSCD LLSAHGNPCM KEKLVMKTHC
260 270 280 290 300
PNDYQSAHHL NNDGKMASVK CPPKYELTED CNFCRQMTGA SLKKGSYPLQ
310 320 330 340 350
DLFCQSSEDD GSKLKTKMKG VCEVGVQALK KCDGQLSTAH EVVPFAVFKN
360 370 380 390 400
SKKVYLDKLD LKTEENLLPD SFVCFEHKGQ YKGTMDSGQT KRELKSFDIS
410 420 430 440 450
QCPKIGGHGS KKCTGDAAFC SAYECTAQYA NAYCSHANGS GIVQIQVSGV
460 470 480 490 500
WKKPLCVGYE RVVVKRELSA KPIQRVEPCT TCITKCEPHG LVVRSTGFKI
510 520 530 540 550
SSAVACASGV CVTGSQSPST EITLKYPGIS QSSGGDIGVH MAHDDQSVSS
560 570 580 590 600
KIVAHCPPQD PCLVHDCIVC AHGLINYQCH TALSAFVVVF VFSSIAIICL
610 620 630 640 650
AILYRVLKCL KIAPRKVLNP LMWITAFIRW IYKKMVARVA DNINQVNREI
660 670 680 690 700
GWMEGGQLVL GNPAPIPRHA PIPRYSTYLM LLLIVSYASA CSELIQASSR
710 720 730 740 750
ITTCSTEGVN TKCRLSGTAL IRAGSVGAEA CLMLKGVKED QTKFLKLKTV
760 770 780 790 800
SSELSCREGQ SYWTGSFSPK CLSSRRCHLV GECHVNRCLS WRDNETSAEF
810 820 830 840 850
SFVGESTTMR ENKCFEQCGG WGCGCFNVNP SCLFVHTYLQ SVRKEALRVF
860 870 880 890 900
NCIDWVHKLT LEITDFDGSV STIDLGASSS RFTNWGSVSL SLDAEGISGS
910 920 930 940 950
NSFSFIESPG KGYAIVDEPF SEIPRQGFLG EIRCNSESSV LSAHESCLRA
960 970 980 990 1000
PNLISYKPMI DQLECTTNLI DPFVVFERGS LPQTRNDKTF AASKGNRGVQ
1010 1020 1030 1040 1050
AFSKGSVQAD LTLMFDNFEV DFVGAAVSCD AAFLNLTGCY SCNAGARVCL
1060 1070 1080 1090 1100
SITSTGTGSL SAHNKDGSLH IVLPSENGTK DQCQILHFTV PEVEEEFMYS
1110 1120 1130 1140 1150
CDGDERPLLV KGTLIAIDPF DDRREAGGES TVVNPKSGSW NFFDWFSGLM
1160 1170 1180 1190
SWFGGPLKTI LLICLYVALS IGLFFLLIYL GGTGLSKMWL AATKKAS
Length:1,197
Mass (Da):130,805
Last modified:May 1, 1991 - v1
Checksum:i860B822CD968767F
GO
Isoform NSm protein1 Publication (identifier: P21401-3) [UniParc]FASTAAdd to basket
Also known as: P14

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.
     154-1197: Missing.

Show »
Length:115
Mass (Da):12,615
Checksum:i6A9A17415F6B6232
GO
Isoform NSm' protein1 Publication (identifier: P21401-5) [UniParc]FASTAAdd to basket
Also known as: P13

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.
     154-1197: Missing.

Show »
Length:102
Mass (Da):11,068
Checksum:i0E794648C9D34777
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5151Missing in isoform NSm' protein. VSP_057988Add
BLAST
Alternative sequencei1 – 3838Missing in isoform NSm protein. VSP_057989Add
BLAST
Alternative sequencei154 – 11971044Missing in isoform NSm protein and isoform NSm' protein. VSP_057990Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25276 Genomic RNA. Translation: AAA47449.1.
PIRiA30183. VGVURF.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25276 Genomic RNA. Translation: AAA47449.1.
PIRiA30183. VGVURF.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP21401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR016404. M_polyprot_prcur_phlebovir.
IPR010826. Phlebovirus_G1.
IPR009878. Phlebovirus_G2.
IPR009879. Phlebovirus_NSM.
[Graphical view]
PfamiPF07243. Phlebovirus_G1. 1 hit.
PF07245. Phlebovirus_G2. 1 hit.
PF07246. Phlebovirus_NSM. 2 hits.
[Graphical view]
PIRSFiPIRSF003961. M_poly_PhleboV. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGP_RVFVZ
AccessioniPrimary (citable) accession number: P21401
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.