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P21400 (GP_PUUMH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Envelope glycoprotein
Short name=GP
Alternative name(s):
M polyprotein

Cleaved into the following 2 chains:

  1. Glycoprotein G1
  2. Glycoprotein G2
Gene names
Name:GP
OrganismPuumala virus (strain Hallnas B1) (Nephropathia epidemica virus) [Complete proteome]
Taxonomic identifier11605 [NCBI]
Taxonomic lineageViruses › ssRNA negative-strand viruses › Bunyaviridae › Hantavirus ›
Virus hostHomo sapiens (Human) [TaxID: 9606]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]

Protein attributes

Sequence length1148 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion. G1 contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis By similarity.

Subunit structure

G1 and G2 interacts with each other By similarity.

Subcellular location

Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs By similarity.

Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential.

Post-translational modification

Specific enzymatic cleavages yield mature proteins including glycoprotein G1 and glycoprotein G2.

Sequence similarities

Belongs to the hantavirus envelope glycoprotein family.

Contains 1 ITAM domain.

Ontologies

Keywords
   Biological processFusion of virus membrane with host endosomal membrane
Fusion of virus membrane with host membrane
Host-virus interaction
Inhibition of host IFN-mediated response initiation by virus
Inhibition of host TRAFs by virus
Inhibition of host innate immune response by virus
Viral attachment to host cell
Viral immunoevasion
Viral penetration into host cytoplasm
Virus entry into host cell
   Cellular componentHost Golgi apparatus
Host endoplasmic reticulum
Host membrane
Membrane
Virion
   DomainSignal
Transmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processsignal transduction

Inferred from electronic annotation. Source: InterPro

suppression by virus of host TRAF activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon production

Inferred from electronic annotation. Source: UniProtKB-KW

viral attachment to host cell

Inferred from electronic annotation. Source: UniProtKB-KW

viral entry into host cell

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell Golgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

virion membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 11481125Envelope glycoprotein
PRO_0000036822
Chain24 – 658635Glycoprotein G1 By similarity
PRO_0000036823
Chain659 – 1148490Glycoprotein G2 By similarity
PRO_0000036824

Regions

Topological domain24 – 495472Lumenal Potential
Transmembrane496 – 51621Helical; Potential
Topological domain517 – 658142Cytoplasmic Potential
Topological domain659 – 1114456Lumenal Potential
Transmembrane1115 – 113521Helical; Potential
Topological domain1136 – 114813Cytoplasmic Potential
Domain621 – 64424ITAM

Sites

Site658 – 6592Cleavage; by host signal peptidase By similarity

Amino acid modifications

Glycosylation1421N-linked (GlcNAc...); by host Potential
Glycosylation3571N-linked (GlcNAc...); by host Potential
Glycosylation4091N-linked (GlcNAc...); by host Potential
Glycosylation8981N-linked (GlcNAc...); by host Potential
Glycosylation9371N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P21400 [UniParc].

Last modified August 1, 1992. Version 2.
Checksum: 52FE60F120C01AAB

FASTA1,148126,907
        10         20         30         40         50         60 
MGELSPVCLY LLLQGLLLCN TGAARNLNEL KMECPHTIRL GQGLVVGSVE LPSLPIQQVE 

        70         80         90        100        110        120 
TLKLESSCNF DLHTSTAGQQ SFTKWTWEIK GDLAENTQAS STSFQTKSSE VNLRGLCLIP 

       130        140        150        160        170        180 
TLVVETAARM RKTIACYDLS CNQTVCQPTV YLMGPIQTCI TTKSCLLSLG DQRIQVNYEK 

       190        200        210        220        230        240 
TYCVSGDLVE GICFNPIHTM ALSQPSHTYD IMTMMVRCFL VIKKVTSGDS MKIEKNFETL 

       250        260        270        280        290        300 
VQKNGCTANN FQGYYICLIG SSSEPLYVPA LDDYRSAEVL SRMAFAPHGE DHDIEKNAVS 

       310        320        330        340        350        360 
AMRIAGKVTG KVPSTESSDT VQGIAFSGSP LYTSTGVLTS KDDPVYIWAP GIIMEGNHSI 

       370        380        390        400        410        420 
CEKKTLPLTW TGFISLPGEI EKTTQCTVFC TLAGPGADCE AYSETGIFNI SSPTCLINRV 

       430        440        450        460        470        480 
QRFRGSEQQI KFVCQRVDMD ITVYCNGMKK VILTKTLVIG QCIYTFTSIF SLIPGVAHSL 

       490        500        510        520        530        540 
AVELCVPGLH GWATMLLLLT FCFGWVLIPT ITMILLKILI AFAYLCSKYN TDSKFRILIE 

       550        560        570        580        590        600 
KVKREYQKTM GSMVCEVCQY ECETAKELES HRKSCSIGSC PYCLNPSEAT TSALQAHFKV 

       610        620        630        640        650        660 
CKLRSRFQEN LRKSLTVYEP MQGCYRTLSL FRYRSRFFVG LVWCVLLVHH LIVWAASAET 

       670        680        690        700        710        720 
QNLNAGWTDT AHGSRIIPMK TDLELDFSLQ SSASYTYRRQ LQNPANEQEK IPFHLQLSKQ 

       730        740        750        760        770        780 
VIHAEIQHLG HWMDATFNLK TAFHCYGSCE KYAYPWQTAG CFIEKDYEYE TGWGCNPPDC 

       790        800        810        820        830        840 
PGVGTGCTAC GVYLDKLKSV GKVFKIVSLR YTRKVCIQLG TEQTCKTVDS NDCLITTSVK 

       850        860        870        880        890        900 
VCLIGTISKF QPSDTLLFLG PLQQGGLIFK QWCTTTCQFG DPGDIMSTPT GMKCPELNGS 

       910        920        930        940        950        960 
FRKKCAFATT PVCQFDGNTI SGYKRMIATK DSFQSFNVTE PHISTSALEW IDPDSSLRDH 

       970        980        990       1000       1010       1020 
INVIVSRDLS FQDLSETPCQ IDLATASIDG AWGSGVGFNL VCTVSLTECS AFLTSIKACD 

      1030       1040       1050       1060       1070       1080 
AAMCYGSTTA NLVRGQNTIH IVGKGGHSGS KFMCCHDTKC SSTGLVAAAP HLDRVTPYNQ 

      1090       1100       1110       1120       1130       1140 
ADSDKIFDDG APECGMSCWF KKSGEWILGV LNGNWMVVAV LVVLLILSIL LFTLCCPRRP 


SYRKEHKP

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References

[1]"Determination of the coding capacity of the M genome segment of nephropathia epidemica virus strain Hallnas B1 by molecular cloning and nucleotide sequence analysis."
Giebel L.B., Stohwasser R., Zoeller L., Bautz E.K.F., Darai G.
Virology 172:498-505(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Comparison of the deduced gene products of the L, M and S genome segments of hantaviruses."
Antic D., Kang C.Y., Spik K., Schmaljohn C.S., Vapalahti O., Vaheri A.
Virus Res. 24:35-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 802-803.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M29979 Genomic RNA. No translation available.
PIRGNVUNE. A33077.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR016402. Envelope_gylcoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view]
PfamPF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view]
PIRSFPIRSF003945. M_poly_HantaV. 1 hit.
ProDomPD001813. Hanta_G2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS51055. ITAM_1. False negative.
PS51056. ITAM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGP_PUUMH
AccessionPrimary (citable) accession number: P21400
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 1, 1992
Last modified: April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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