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P21396

- AOFA_RAT

UniProt

P21396 - AOFA_RAT

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Protein
Amine oxidase [flavin-containing] A
Gene
Maoa
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

Catalytic activityi

RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.

Cofactori

FAD.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei335 – 3351Important for substrate specificity
Sitei374 – 3741Important for catalytic activity By similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: RGD
  2. primary amine oxidase activity Source: RGD
  3. serotonin binding Source: RGD
Complete GO annotation...

GO - Biological processi

  1. catecholamine catabolic process Source: RGD
  2. neurotransmitter catabolic process Source: UniProtKB-KW
  3. phenylethylamine metabolic process Source: RGD
  4. serotonin metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Catecholamine metabolism, Neurotransmitter degradation

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14994.
BRENDAi1.4.3.4. 5301.
SABIO-RKP21396.

Names & Taxonomyi

Protein namesi
Recommended name:
Amine oxidase [flavin-containing] A (EC:1.4.3.4)
Alternative name(s):
Monoamine oxidase type A
Short name:
MAO-A
Gene namesi
Name:Maoa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61898. Maoa.

Subcellular locationi

Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 497497Cytoplasmic
Add
BLAST
Transmembranei498 – 51821Helical; Anchor for type IV membrane protein
Add
BLAST
Topological domaini519 – 5268Mitochondrial intermembrane

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial outer membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi208 – 2081F → A: Lower affinity for serotonin and tyramine; no change for tryptamine. 1 Publication
Mutagenesisi208 – 2081F → I: Lower affinity for serotonin and tyramine; no change for tryptamine. 1 Publication
Mutagenesisi208 – 2081F → V: Lower affinity for serotonin and tyramine; no change for tryptamine. 1 Publication
Mutagenesisi208 – 2081F → Y: No change in substrate affinity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Amine oxidase [flavin-containing] A
PRO_0000099854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei406 – 4061S-8alpha-FAD cysteine

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP21396.
PRIDEiP21396.

PTM databases

PhosphoSiteiP21396.

Expressioni

Gene expression databases

GenevestigatoriP21396.

Interactioni

Subunit structurei

Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 197
Helixi23 – 3412
Beta strandi39 – 424
Beta strandi44 – 485
Turni58 – 603
Beta strandi63 – 664
Helixi75 – 839
Beta strandi88 – 903
Beta strandi94 – 10310
Beta strandi105 – 1084
Beta strandi110 – 1123
Helixi118 – 13619
Helixi143 – 1453
Helixi149 – 1524
Helixi157 – 1648
Helixi168 – 18215
Turni186 – 1883
Helixi191 – 20010
Helixi203 – 2086
Beta strandi210 – 2134
Beta strandi216 – 2194
Helixi225 – 2328
Helixi235 – 2373
Beta strandi238 – 2414
Beta strandi244 – 2485
Beta strandi250 – 26112
Beta strandi263 – 2719
Helixi275 – 2806
Beta strandi281 – 2855
Helixi289 – 2946
Beta strandi303 – 3097
Helixi316 – 3183
Beta strandi320 – 3278
Beta strandi334 – 3385
Beta strandi348 – 3547
Helixi355 – 3628
Helixi366 – 38116
Helixi384 – 3874
Beta strandi390 – 3967
Turni397 – 3993
Turni401 – 4033
Beta strandi405 – 4073
Turni413 – 4153
Helixi416 – 4194
Helixi420 – 4223
Beta strandi428 – 4325
Helixi435 – 4373
Beta strandi439 – 4413
Helixi445 – 46117
Turni462 – 4643
Beta strandi479 – 4813
Helixi490 – 4945
Helixi498 – 51922

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1O5WX-ray3.20A/B/C/D1-526[»]
ProteinModelPortaliP21396.
SMRiP21396. Positions 10-521.

Miscellaneous databases

EvolutionaryTraceiP21396.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni520 – 5223Interaction with membrane phospholipid headgroups By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1231.
HOGENOMiHOG000221615.
HOVERGENiHBG004255.
InParanoidiP21396.
PhylomeDBiP21396.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.

Sequencei

Sequence statusi: Complete.

P21396-1 [UniParc]FASTAAdd to Basket

« Hide

MTDLEKPNLA GHMFDVGLIG GGISGLAAAK LLSEYKINVL VLEARDRVGG    50
RTYTVRNEHV KWVDVGGAYV GPTQNRILRL SKELGIETYK VNVNERLVQY 100
VKGKTYPFRG AFPPVWNPLA YLDYNNLWRT MDEMGKEIPV DAPWQARHAQ 150
EWDKMTMKDL IDKICWTKTA REFAYLFVNI NVTSEPHEVS ALWFLWYVRQ 200
CGGTARIFSV TNGGQERKFV GGSGQVSEQI MGLLGDKVKL SSPVTYIDQT 250
DDNIIVETLN HEHYECKYVI SAIPPILTAK IHFKPELPPE RNQLIQRLPM 300
GAVIKCMVYY KEAFWKKKDY CGCMIIEDEE APIAITLDDT KPDGSLPAIM 350
GFILARKADR QAKLHKDIRK RKICELYAKV LGSQEALYPV HYEEKNWCEE 400
QYSGGCYTAY FPPGIMTQYG RVIRQPVGRI YFAGTETATQ WSGYMEGAVE 450
AGERAAREVL NALGKVAKKD IWVEEPESKD VPAIEITHTF LERNLPSVPG 500
LLKITGVSTS VALLCFVLYK IKKLPC 526
Length:526
Mass (Da):59,508
Last modified:May 1, 1991 - v1
Checksum:i381AE35D5A73E25B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 182GL → VV in AAB23355. 1 Publication
Sequence conflicti361 – 3611Q → L in AAB23355. 1 Publication
Sequence conflicti406 – 4061C → S in AAB23355. 1 Publication
Sequence conflicti451 – 4511A → R in AAB23355. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00688 mRNA. Translation: BAA00592.1.
S45812 mRNA. Translation: AAB23355.2.
PIRiJT0528.
UniGeneiRn.224544.

Genome annotation databases

UCSCiRGD:61898. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00688 mRNA. Translation: BAA00592.1 .
S45812 mRNA. Translation: AAB23355.2 .
PIRi JT0528.
UniGenei Rn.224544.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1O5W X-ray 3.20 A/B/C/D 1-526 [» ]
ProteinModelPortali P21396.
SMRi P21396. Positions 10-521.
ModBasei Search...

Chemistry

BindingDBi P21396.
ChEMBLi CHEMBL2095196.

PTM databases

PhosphoSitei P21396.

Proteomic databases

PaxDbi P21396.
PRIDEi P21396.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:61898. rat.

Organism-specific databases

RGDi 61898. Maoa.

Phylogenomic databases

eggNOGi COG1231.
HOGENOMi HOG000221615.
HOVERGENi HBG004255.
InParanoidi P21396.
PhylomeDBi P21396.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-14994.
BRENDAi 1.4.3.4. 5301.
SABIO-RK P21396.

Miscellaneous databases

EvolutionaryTracei P21396.
PROi P21396.

Gene expression databases

Genevestigatori P21396.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
PRINTSi PR00757. AMINEOXDASEF.
ProtoNeti Search...

Publicationsi

  1. "Primary structure of rat monoamine oxidase A deduced from cDNA and its expression in rat tissues."
    Kuwahara T., Takamoto S., Ito A.
    Agric. Biol. Chem. 54:253-257(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "cDNA cloning and sequencing of rat monoamine oxidase A: comparison with the human and bovine enzymes."
    Kwan S.W., Abell C.W.
    Comp. Biochem. Physiol. 102B:143-147(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-526.
    Tissue: Liver.
  3. "A key amino acid responsible for substrate selectivity of monoamine oxidase A and B."
    Tsugeno Y., Ito A.
    J. Biol. Chem. 272:14033-14036(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PHE-208.
  4. "Structure of rat monoamine oxidase A and its specific recognitions for substrates and inhibitors."
    Ma J., Yoshimura M., Yamashita E., Nakagawa A., Ito A., Tsukihara T.
    J. Mol. Biol. 338:103-114(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH INHIBITOR, SUBUNIT, COFACTOR, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiAOFA_RAT
AccessioniPrimary (citable) accession number: P21396
Secondary accession number(s): Q63817
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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