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P21396

- AOFA_RAT

UniProt

P21396 - AOFA_RAT

Protein

Amine oxidase [flavin-containing] A

Gene

Maoa

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

    Catalytic activityi

    RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.

    Cofactori

    FAD.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei335 – 3351Important for substrate specificity
    Sitei374 – 3741Important for catalytic activityBy similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: RGD
    2. primary amine oxidase activity Source: RGD
    3. serotonin binding Source: RGD

    GO - Biological processi

    1. catecholamine catabolic process Source: RGD
    2. neurotransmitter catabolic process Source: UniProtKB-KW
    3. phenylethylamine metabolic process Source: RGD
    4. serotonin metabolic process Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Catecholamine metabolism, Neurotransmitter degradation

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14994.
    BRENDAi1.4.3.4. 5301.
    SABIO-RKP21396.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amine oxidase [flavin-containing] A (EC:1.4.3.4)
    Alternative name(s):
    Monoamine oxidase type A
    Short name:
    MAO-A
    Gene namesi
    Name:Maoa
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61898. Maoa.

    Subcellular locationi

    Mitochondrion outer membrane 1 Publication; Single-pass type IV membrane protein 1 Publication; Cytoplasmic side 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial outer membrane Source: RGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi208 – 2081F → A: Lower affinity for serotonin and tyramine; no change for tryptamine. 1 Publication
    Mutagenesisi208 – 2081F → I: Lower affinity for serotonin and tyramine; no change for tryptamine. 1 Publication
    Mutagenesisi208 – 2081F → V: Lower affinity for serotonin and tyramine; no change for tryptamine. 1 Publication
    Mutagenesisi208 – 2081F → Y: No change in substrate affinity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526Amine oxidase [flavin-containing] APRO_0000099854Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei406 – 4061S-8alpha-FAD cysteine

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP21396.
    PRIDEiP21396.

    PTM databases

    PhosphoSiteiP21396.

    Expressioni

    Gene expression databases

    GenevestigatoriP21396.

    Interactioni

    Subunit structurei

    Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.By similarity

    Structurei

    Secondary structure

    1
    526
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi13 – 197
    Helixi23 – 3412
    Beta strandi39 – 424
    Beta strandi44 – 485
    Turni58 – 603
    Beta strandi63 – 664
    Helixi75 – 839
    Beta strandi88 – 903
    Beta strandi94 – 10310
    Beta strandi105 – 1084
    Beta strandi110 – 1123
    Helixi118 – 13619
    Helixi143 – 1453
    Helixi149 – 1524
    Helixi157 – 1648
    Helixi168 – 18215
    Turni186 – 1883
    Helixi191 – 20010
    Helixi203 – 2086
    Beta strandi210 – 2134
    Beta strandi216 – 2194
    Helixi225 – 2328
    Helixi235 – 2373
    Beta strandi238 – 2414
    Beta strandi244 – 2485
    Beta strandi250 – 26112
    Beta strandi263 – 2719
    Helixi275 – 2806
    Beta strandi281 – 2855
    Helixi289 – 2946
    Beta strandi303 – 3097
    Helixi316 – 3183
    Beta strandi320 – 3278
    Beta strandi334 – 3385
    Beta strandi348 – 3547
    Helixi355 – 3628
    Helixi366 – 38116
    Helixi384 – 3874
    Beta strandi390 – 3967
    Turni397 – 3993
    Turni401 – 4033
    Beta strandi405 – 4073
    Turni413 – 4153
    Helixi416 – 4194
    Helixi420 – 4223
    Beta strandi428 – 4325
    Helixi435 – 4373
    Beta strandi439 – 4413
    Helixi445 – 46117
    Turni462 – 4643
    Beta strandi479 – 4813
    Helixi490 – 4945
    Helixi498 – 51922

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1O5WX-ray3.20A/B/C/D1-526[»]
    ProteinModelPortaliP21396.
    SMRiP21396. Positions 10-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21396.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 497497CytoplasmicAdd
    BLAST
    Topological domaini519 – 5268Mitochondrial intermembrane

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei498 – 51821Helical; Anchor for type IV membrane proteinAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni520 – 5223Interaction with membrane phospholipid headgroupsBy similarity

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1231.
    HOGENOMiHOG000221615.
    HOVERGENiHBG004255.
    InParanoidiP21396.
    PhylomeDBiP21396.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.

    Sequencei

    Sequence statusi: Complete.

    P21396-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDLEKPNLA GHMFDVGLIG GGISGLAAAK LLSEYKINVL VLEARDRVGG    50
    RTYTVRNEHV KWVDVGGAYV GPTQNRILRL SKELGIETYK VNVNERLVQY 100
    VKGKTYPFRG AFPPVWNPLA YLDYNNLWRT MDEMGKEIPV DAPWQARHAQ 150
    EWDKMTMKDL IDKICWTKTA REFAYLFVNI NVTSEPHEVS ALWFLWYVRQ 200
    CGGTARIFSV TNGGQERKFV GGSGQVSEQI MGLLGDKVKL SSPVTYIDQT 250
    DDNIIVETLN HEHYECKYVI SAIPPILTAK IHFKPELPPE RNQLIQRLPM 300
    GAVIKCMVYY KEAFWKKKDY CGCMIIEDEE APIAITLDDT KPDGSLPAIM 350
    GFILARKADR QAKLHKDIRK RKICELYAKV LGSQEALYPV HYEEKNWCEE 400
    QYSGGCYTAY FPPGIMTQYG RVIRQPVGRI YFAGTETATQ WSGYMEGAVE 450
    AGERAAREVL NALGKVAKKD IWVEEPESKD VPAIEITHTF LERNLPSVPG 500
    LLKITGVSTS VALLCFVLYK IKKLPC 526
    Length:526
    Mass (Da):59,508
    Last modified:May 1, 1991 - v1
    Checksum:i381AE35D5A73E25B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 182GL → VV in AAB23355. (PubMed:1526120)Curated
    Sequence conflicti361 – 3611Q → L in AAB23355. (PubMed:1526120)Curated
    Sequence conflicti406 – 4061C → S in AAB23355. (PubMed:1526120)Curated
    Sequence conflicti451 – 4511A → R in AAB23355. (PubMed:1526120)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00688 mRNA. Translation: BAA00592.1.
    S45812 mRNA. Translation: AAB23355.2.
    PIRiJT0528.
    UniGeneiRn.224544.

    Genome annotation databases

    UCSCiRGD:61898. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00688 mRNA. Translation: BAA00592.1 .
    S45812 mRNA. Translation: AAB23355.2 .
    PIRi JT0528.
    UniGenei Rn.224544.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1O5W X-ray 3.20 A/B/C/D 1-526 [» ]
    ProteinModelPortali P21396.
    SMRi P21396. Positions 10-521.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P21396.
    ChEMBLi CHEMBL2095196.

    PTM databases

    PhosphoSitei P21396.

    Proteomic databases

    PaxDbi P21396.
    PRIDEi P21396.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:61898. rat.

    Organism-specific databases

    RGDi 61898. Maoa.

    Phylogenomic databases

    eggNOGi COG1231.
    HOGENOMi HOG000221615.
    HOVERGENi HBG004255.
    InParanoidi P21396.
    PhylomeDBi P21396.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-14994.
    BRENDAi 1.4.3.4. 5301.
    SABIO-RK P21396.

    Miscellaneous databases

    EvolutionaryTracei P21396.
    PROi P21396.

    Gene expression databases

    Genevestigatori P21396.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00757. AMINEOXDASEF.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of rat monoamine oxidase A deduced from cDNA and its expression in rat tissues."
      Kuwahara T., Takamoto S., Ito A.
      Agric. Biol. Chem. 54:253-257(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "cDNA cloning and sequencing of rat monoamine oxidase A: comparison with the human and bovine enzymes."
      Kwan S.W., Abell C.W.
      Comp. Biochem. Physiol. 102B:143-147(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-526.
      Tissue: Liver.
    3. "A key amino acid responsible for substrate selectivity of monoamine oxidase A and B."
      Tsugeno Y., Ito A.
      J. Biol. Chem. 272:14033-14036(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF PHE-208.
    4. "Structure of rat monoamine oxidase A and its specific recognitions for substrates and inhibitors."
      Ma J., Yoshimura M., Yamashita E., Nakagawa A., Ito A., Tsukihara T.
      J. Mol. Biol. 338:103-114(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH INHIBITOR, SUBUNIT, COFACTOR, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiAOFA_RAT
    AccessioniPrimary (citable) accession number: P21396
    Secondary accession number(s): Q63817
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3