P21395 (XYLM_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xylene monooxygenase subunit 1
EC=1.14.15.-

Gene names

Name:

xylM

Encoded on

Plasmid TOL pWW0

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidizes toluene and xylenes to (methyl)benzyl alcohols. The enzyme has a broad specificity and also oxidizes (methyl)benzyl alcohols to (methyl)benzaldehydes and indole to indoxyl.
Cofactor	Binds 2 Fe3+ ions per subunit By similarity.
Subunit structure	Composed of two subunits: xylA and xylM.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the fatty acid desaturase family. AlkB subfamily.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Monooxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Xylene monooxygenase subunit 1		PRO_0000185435
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	60 – 80	21	Helical; Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Transmembrane	207 – 227	21	Helical; Potential
	Transmembrane	305 – 325	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P21395 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: AB76149B37DF7794	FASTA	369	41,566
	10 20 30 40 50 60 MDTLRYYLIP VVTACGLIGF YYGGYWVWLG AATFPALMVL DVILPKDFSA RKVSPFFADL 70 80 90 100 110 120 TQYLQLPLMI GLYGLLVFGV ENGRIELSEP LQVAGCILSL AWLSGVPTLP VSHELMHRRH 130 140 150 160 170 180 WLPRKMAQLL AMFYGDPNRD IAHVNTHHLY LDTPLDSDTP YRGQTIYSFV ISATVGSVKD 190 200 210 220 230 240 AIKIEAETLR RKGQSPWNLS NKTYQYVALL LALPGLVSYL GGPALGLVTI ASMIIAKGIV 250 260 270 280 290 300 EGFNYFQHYG LVRDLDQPIL LHHAWNHMGT IVRPLGCEIT NHINHHIDGY TRFYELRPEK 310 320 330 340 350 360 EAPQMPSLFV CFLLGLIPPL WFALIAKPKL RDWDQRYATP GERELAMAAN KKAGWPLWCE SELGRVASI

References

[1]	"Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system." Suzuki M., Hayakawa T., Shaw J.P., Rekik M., Harayama S. J. Bacteriol. 173:1690-1695(1991) [PubMed: 1999388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37480 Genomic DNA. Translation: AAA26026.1. D63341 Genomic DNA. Translation: BAA09662.1.
PIR	A37316.
RefSeq	NP_542887.1. NC_003350.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1218754.
Phylogenomic databases
ProtClustDB	CLSK782454.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-2942.
Family and domain databases
InterPro	IPR005804. Fatty_acid_desaturase-1. [Graphical view]
Pfam	PF00487. FA_desaturase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLM_PSEPU

Accession

Primary (citable) accession number: P21395

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	June 28, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program