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Protein

Xylene monooxygenase electron transfer component

Gene

xylA

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidizes toluene and xylenes to (methyl)benzyl alcohols. The enzyme has a broad specificity and also oxidizes (methyl)benzyl alcohols to (methyl)benzaldehydes and indole to indoxyl.

Catalytic activityi

Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi57Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi60Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi92Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAromatic hydrocarbons catabolism
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2941

Names & Taxonomyi

Protein namesi
Recommended name:
Xylene monooxygenase electron transfer component
Including the following 2 domains:
Ferredoxin
Ferredoxin--NAD(+) reductase (EC:1.18.1.3)
Gene namesi
Name:xylA
Encoded oniPlasmid TOL pWW00 Publication
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001676601 – 350Xylene monooxygenase electron transfer componentAdd BLAST350

Interactioni

Subunit structurei

Composed of two subunits: XylA and XylM.

Structurei

3D structure databases

ProteinModelPortaliP21394
SMRiP21394
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 1082Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST93
Domaini114 – 213FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 350Ferredoxin--NADH reductaseAdd BLAST242

Sequence similaritiesi

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR001221 Phe_hydroxylase
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00111 Fer2, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00371 FPNCR
PR00410 PHEHYDRXLASE
SUPFAMiSSF54292 SSF54292, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

P21394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEFFKKISG LFVPPPESTV SVRGQGFQFK VPRGQTILES ALHQGIAFPH
60 70 80 90 100
DCKVGSCGTC KYKLISGRVN ELTSSAMGLS GDLYQSGYRL GCQCIPKEDL
110 120 130 140 150
EIELDTVLGQ ALVPIETSAL ISKQKRLAHD IVEMEVVPDK QIAFYPGQYA
160 170 180 190 200
DVECAECSAV RSYSFSAPPQ PDGSLSFHVR LVPGGVFSGW LFGGDRTGAT
210 220 230 240 250
LTLRAPYGQF GLHESNATMV CVAGGTGLAP IKCVLQSMTQ AQRERDVLLF
260 270 280 290 300
FGARQQRDLY CLDEIEALQL DWGGRFELIP VLSEESSTSS WKGKRGMVTE
310 320 330 340 350
YFKEYLTGQP YEGYLCGPPP MVDAAETELV RLGVARELVF ADRFYNRPPC
Length:350
Mass (Da):38,455
Last modified:May 1, 1991 - v1
Checksum:i26828AC2226C1DDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37480 Genomic DNA Translation: AAA26027.1
D63341 Genomic DNA Translation: BAA09663.1
PIRiB37316
RefSeqiNP_542886.1, NC_003350.1
WP_011005929.1, NC_003350.1

Genome annotation databases

GeneIDi1218743

Similar proteinsi

Entry informationi

Entry nameiXYLA_PSEPU
AccessioniPrimary (citable) accession number: P21394
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid
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Main funding by: National Institutes of Health