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Protein

Fumarate reductase 2

Gene

OSM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Irreversibly catalyzes the reduction of fumarate to succinate. Together with the second isozyme of soluble fumarate reductase (FRD1), essential for anaerobic growth. Involved in maintaining redox balance during oxygen deficiency conditions. Reduction of fumarate is the main source of succinate during fermentation, and under anaerobic conditions, the formation of succinate is strictly required for the reoxidation of FADH2.4 Publications

Catalytic activityi

Succinate + NAD+ = fumarate + NADH.1 Publication

Cofactori

FAD1 PublicationNote: Binds 1 FAD per monomer.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei281 – 2811By similarity
Active sitei304 – 3041By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 5115FADSequence analysisAdd
BLAST

GO - Molecular functioni

  • fumarate reductase (NADH) activity Source: SGD
  • succinate dehydrogenase activity Source: InterPro

GO - Biological processi

  • FAD metabolic process Source: SGD
  • protein folding in endoplasmic reticulum Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciYEAST:YJR051W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate reductase 2 (EC:1.3.1.6)
Short name:
FRDS2
Alternative name(s):
NADH-dependent fumarate reductase
Osmotic sensitivity protein 1
Soluble fumarate reductase, mitochondrial isozyme
Gene namesi
Name:OSM1
Ordered Locus Names:YJR051W
ORF Names:J1659
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR051W.
SGDiS000003812. OSM1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Causes increased sensitivity to hypertonic growth medium.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232Mitochondrion1 PublicationAdd
BLAST
Chaini33 – 501469Fumarate reductase 2PRO_0000158668Add
BLAST

Proteomic databases

MaxQBiP21375.
PeptideAtlasiP21375.

PTM databases

iPTMnetiP21375.

Interactioni

Protein-protein interaction databases

BioGridi33804. 41 interactions.
DIPiDIP-5426N.
IntActiP21375. 2 interactions.
MINTiMINT-494436.

Structurei

3D structure databases

ProteinModelPortaliP21375.
SMRiP21375. Positions 20-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00650000094148.
HOGENOMiHOG000227327.
InParanoidiP21375.
KOiK18561.
OMAiWKGLMRK.
OrthoDBiEOG7T1RMD.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR010960. Flavocytochrome_c.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01813. flavo_cyto_c. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRSVRRVFI YVSIFVLIIV LKRTLSGTDQ TSMKQPVVVI GSGLAGLTTS
60 70 80 90 100
NRLISKYRIP VVLLDKAASI GGNSIKASSG INGAHTDTQQ NLKVMDTPEL
110 120 130 140 150
FLKDTLHSAK GRGVPSLMDK LTKESKSAIR WLQTEFDLKL DLLAQLGGHS
160 170 180 190 200
VPRTHRSSGK LPPGFEIVQA LSKKLKDISS KDSNLVQIML NSEVVDIELD
210 220 230 240 250
NQGHVTGVVY MDENGNRKIM KSHHVVFCSG GFGYSKEMLK EYSPNLIHLP
260 270 280 290 300
TTNGKQTTGD GQKILSKLGA ELIDMDQVQV HPTGFIDPND RENNWKFLAA
310 320 330 340 350
EALRGLGGIL LHPTTGRRFT NELSTRDTVT MEIQSKCPKN DNRALLVMSD
360 370 380 390 400
KVYENYTNNI NFYMSKNLIK KVSINDLIRQ YDLQTTASEL VTELKSYSDV
410 420 430 440 450
NTKDTFDRPL IINAFDKDIS TESTVYVGEV TPVVHFTMGG VKINEKSQVI
460 470 480 490 500
KKNSESVLSN GIFAAGEVSG GVHGANRLGG SSLLECVVFG KTAADNIAKL

Y
Length:501
Mass (Da):55,065
Last modified:February 1, 1995 - v3
Checksum:iDDBF803BC342A5F9
GO

Sequence cautioni

The sequence AAB59346.1 differs from that shown. Reason: Frameshift at positions 281 and 483. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1072LH → FD in AAB59346 (PubMed:2158927).Curated
Sequence conflicti170 – 1701A → AR in AAB59346 (PubMed:2158927).Curated
Sequence conflicti191 – 1922Missing in AAB59346 (PubMed:2158927).Curated
Sequence conflicti439 – 4391G → S in AAB59346 (PubMed:2158927).Curated
Sequence conflicti456 – 4572SV → TL in AAB59346 (PubMed:2158927).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37696 Genomic DNA. Translation: AAB59346.1. Frameshift.
L26347 Genomic DNA. Translation: AAA62859.1.
L36344 Genomic DNA. Translation: AAA88754.1.
Z49551 Genomic DNA. Translation: CAA89579.1.
BK006943 Genomic DNA. Translation: DAA08838.1.
PIRiS46591.
RefSeqiNP_012585.1. NM_001181709.1.

Genome annotation databases

EnsemblFungiiYJR051W; YJR051W; YJR051W.
GeneIDi853510.
KEGGisce:YJR051W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37696 Genomic DNA. Translation: AAB59346.1. Frameshift.
L26347 Genomic DNA. Translation: AAA62859.1.
L36344 Genomic DNA. Translation: AAA88754.1.
Z49551 Genomic DNA. Translation: CAA89579.1.
BK006943 Genomic DNA. Translation: DAA08838.1.
PIRiS46591.
RefSeqiNP_012585.1. NM_001181709.1.

3D structure databases

ProteinModelPortaliP21375.
SMRiP21375. Positions 20-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33804. 41 interactions.
DIPiDIP-5426N.
IntActiP21375. 2 interactions.
MINTiMINT-494436.

PTM databases

iPTMnetiP21375.

Proteomic databases

MaxQBiP21375.
PeptideAtlasiP21375.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR051W; YJR051W; YJR051W.
GeneIDi853510.
KEGGisce:YJR051W.

Organism-specific databases

EuPathDBiFungiDB:YJR051W.
SGDiS000003812. OSM1.

Phylogenomic databases

GeneTreeiENSGT00650000094148.
HOGENOMiHOG000227327.
InParanoidiP21375.
KOiK18561.
OMAiWKGLMRK.
OrthoDBiEOG7T1RMD.

Enzyme and pathway databases

BioCyciYEAST:YJR051W-MONOMER.

Miscellaneous databases

NextBioi974171.
PROiP21375.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR010960. Flavocytochrome_c.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01813. flavo_cyto_c. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the COR region: a cluster of six genes in the yeast Saccharomyces cerevisiae."
    Melnick L., Sherman F.
    Gene 87:157-166(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 10:811-818(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "One of the fumarate reductase isoenzymes from Saccharomyces cerevisiae is encoded by the OSM1 gene."
    Muratsubaki H., Enomoto K.
    Arch. Biochem. Biophys. 352:175-181(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 33-52 AND 487-501, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBCELLULAR LOCATION.
  6. "Deletions of the iso-1-cytochrome c and adjacent genes of yeast: discovery of the OSM1 gene controlling osmotic sensitivity."
    Singh A., Sherman F.
    Genetics 89:653-665(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Soluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth."
    Arikawa Y., Enomoto K., Muratsubaki H., Okazaki M.
    FEMS Microbiol. Lett. 165:111-116(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Investigation by 13C-NMR and tricarboxylic acid (TCA) deletion mutant analysis of pathways for succinate formation in Saccharomyces cerevisiae during anaerobic fermentation."
    Camarasa C., Grivet J.P., Dequin S.
    Microbiology 149:2669-2678(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Role in anaerobiosis of the isoenzymes for Saccharomyces cerevisiae fumarate reductase encoded by OSM1 and FRDS1."
    Camarasa C., Faucet V., Dequin S.
    Yeast 24:391-401(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOSM1_YEAST
AccessioniPrimary (citable) accession number: P21375
Secondary accession number(s): D6VWM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.