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Protein

Fumarate reductase 2

Gene

OSM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Irreversibly catalyzes the reduction of fumarate to succinate. Together with the second isozyme of soluble fumarate reductase (FRD1), essential for anaerobic growth. Involved in maintaining redox balance during oxygen deficiency conditions. Reduction of fumarate is the main source of succinate during fermentation, and under anaerobic conditions, the formation of succinate is strictly required for the reoxidation of FADH2.4 Publications

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Succinate + NAD+ = fumarate + NADH.1 Publication

Cofactori

FAD1 PublicationNote: Binds 1 FAD per monomer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei281By similarity1
Active sitei304By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 51FADSequence analysisAdd BLAST15

GO - Molecular functioni

  • fumarate reductase (NADH) activity Source: SGD
  • succinate dehydrogenase activity Source: InterPro

GO - Biological processi

  • FAD metabolic process Source: SGD
  • protein folding in endoplasmic reticulum Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciYEAST:YJR051W-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate reductase 2 (EC:1.3.1.6)
Short name:
FRDS2
Alternative name(s):
NADH-dependent fumarate reductase
Osmotic sensitivity protein 1
Soluble fumarate reductase, mitochondrial isozyme
Gene namesi
Name:OSM1
Ordered Locus Names:YJR051W
ORF Names:J1659
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR051W
SGDiS000003812 OSM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Causes increased sensitivity to hypertonic growth medium.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32Mitochondrion1 PublicationAdd BLAST32
ChainiPRO_000015866833 – 501Fumarate reductase 2Add BLAST469

Proteomic databases

MaxQBiP21375
PaxDbiP21375
PRIDEiP21375

PTM databases

iPTMnetiP21375

Interactioni

Protein-protein interaction databases

BioGridi33804, 73 interactors
DIPiDIP-5426N
IntActiP21375, 2 interactors
MINTiP21375
STRINGi4932.YJR051W

Structurei

Secondary structure

1501
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 40Combined sources4
Helixi44 – 57Combined sources14
Beta strandi61 – 64Combined sources4
Beta strandi66 – 70Combined sources5
Helixi74 – 76Combined sources3
Helixi87 – 91Combined sources5
Helixi98 – 109Combined sources12
Helixi115 – 123Combined sources9
Helixi125 – 136Combined sources12
Beta strandi142 – 144Combined sources3
Beta strandi154 – 156Combined sources3
Beta strandi158 – 161Combined sources4
Helixi163 – 181Combined sources19
Turni183 – 185Combined sources3
Beta strandi186 – 189Combined sources4
Beta strandi193 – 199Combined sources7
Beta strandi205 – 211Combined sources7
Beta strandi217 – 221Combined sources5
Beta strandi225 – 227Combined sources3
Helixi236 – 242Combined sources7
Helixi244 – 246Combined sources3
Beta strandi251 – 253Combined sources3
Helixi260 – 268Combined sources9
Beta strandi272 – 274Combined sources3
Beta strandi278 – 285Combined sources8
Helixi302 – 305Combined sources4
Beta strandi309 – 311Combined sources3
Turni313 – 315Combined sources3
Helixi326 – 336Combined sources11
Beta strandi339 – 341Combined sources3
Beta strandi344 – 348Combined sources5
Helixi350 – 355Combined sources6
Helixi357 – 365Combined sources9
Beta strandi368 – 373Combined sources6
Helixi374 – 380Combined sources7
Helixi387 – 398Combined sources12
Beta strandi424 – 437Combined sources14
Beta strandi440 – 443Combined sources4
Beta strandi447 – 451Combined sources5
Beta strandi454 – 458Combined sources5
Beta strandi462 – 464Combined sources3
Beta strandi469 – 473Combined sources5
Helixi481 – 500Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GLGX-ray1.80A32-501[»]
ProteinModelPortaliP21375
SMRiP21375
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00650000094148
HOGENOMiHOG000227327
InParanoidiP21375
KOiK18561
OMAiRLMGNAL
OrthoDBiEOG092C1BPA

Family and domain databases

Gene3Di3.50.50.60, 2 hits
3.90.700.10, 1 hit
InterProiView protein in InterPro
IPR003953 FAD-binding_2
IPR036188 FAD/NAD-bd_sf
IPR010960 Flavocytochrome_c
IPR027477 Succ_DH/fumarate_Rdtase_cat_sf
PfamiView protein in Pfam
PF00890 FAD_binding_2, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
SSF56425 SSF56425, 1 hit
TIGRFAMsiTIGR01813 flavo_cyto_c, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRSVRRVFI YVSIFVLIIV LKRTLSGTDQ TSMKQPVVVI GSGLAGLTTS
60 70 80 90 100
NRLISKYRIP VVLLDKAASI GGNSIKASSG INGAHTDTQQ NLKVMDTPEL
110 120 130 140 150
FLKDTLHSAK GRGVPSLMDK LTKESKSAIR WLQTEFDLKL DLLAQLGGHS
160 170 180 190 200
VPRTHRSSGK LPPGFEIVQA LSKKLKDISS KDSNLVQIML NSEVVDIELD
210 220 230 240 250
NQGHVTGVVY MDENGNRKIM KSHHVVFCSG GFGYSKEMLK EYSPNLIHLP
260 270 280 290 300
TTNGKQTTGD GQKILSKLGA ELIDMDQVQV HPTGFIDPND RENNWKFLAA
310 320 330 340 350
EALRGLGGIL LHPTTGRRFT NELSTRDTVT MEIQSKCPKN DNRALLVMSD
360 370 380 390 400
KVYENYTNNI NFYMSKNLIK KVSINDLIRQ YDLQTTASEL VTELKSYSDV
410 420 430 440 450
NTKDTFDRPL IINAFDKDIS TESTVYVGEV TPVVHFTMGG VKINEKSQVI
460 470 480 490 500
KKNSESVLSN GIFAAGEVSG GVHGANRLGG SSLLECVVFG KTAADNIAKL

Y
Length:501
Mass (Da):55,065
Last modified:February 1, 1995 - v3
Checksum:iDDBF803BC342A5F9
GO

Sequence cautioni

The sequence AAB59346 differs from that shown. Reason: Frameshift at positions 281 and 483.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106 – 107LH → FD in AAB59346 (PubMed:2158927).Curated2
Sequence conflicti170A → AR in AAB59346 (PubMed:2158927).Curated1
Sequence conflicti191 – 192Missing in AAB59346 (PubMed:2158927).Curated2
Sequence conflicti439G → S in AAB59346 (PubMed:2158927).Curated1
Sequence conflicti456 – 457SV → TL in AAB59346 (PubMed:2158927).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37696 Genomic DNA Translation: AAB59346.1 Frameshift.
L26347 Genomic DNA Translation: AAA62859.1
L36344 Genomic DNA Translation: AAA88754.1
Z49551 Genomic DNA Translation: CAA89579.1
BK006943 Genomic DNA Translation: DAA08838.1
PIRiS46591
RefSeqiNP_012585.1, NM_001181709.1

Genome annotation databases

EnsemblFungiiYJR051W; YJR051W; YJR051W
GeneIDi853510
KEGGisce:YJR051W

Similar proteinsi

Entry informationi

Entry nameiOSM1_YEAST
AccessioniPrimary (citable) accession number: P21375
Secondary accession number(s): D6VWM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 156 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
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Main funding by: National Institutes of Health