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P21373

- UTR1_YEAST

UniProt

P21373 - UTR1_YEAST

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Protein
NAD(+) kinase
Gene
UTR1, YJR049C, J1655
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically phosphorylates NAD in the presence of ATP, dATP, or CTP as phosphoryl donors.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Kineticsi

  1. KM=0.50 mM for NAD1 Publication
  2. KM=0.60 mM for ATP

pH dependencei

Optimum pH is 8.0.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: SGD
  3. NADH kinase activity Source: SGD

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: SGD
  3. cellular iron ion homeostasis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31684-MONOMER.
YEAST:G3O-31684-MONOMER.
BRENDAi2.7.1.23. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(+) kinase (EC:2.7.1.23)
Alternative name(s):
Unknown transcript 1 protein
Gene namesi
Name:UTR1
Ordered Locus Names:YJR049C
ORF Names:J1655
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR049c.
SGDiS000003810. UTR1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530NAD(+) kinaseUniRule annotation
PRO_0000120716Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei499 – 4991Phosphoserine2 Publications
Modified residuei503 – 5031Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21373.
PaxDbiP21373.

Expressioni

Gene expression databases

GenevestigatoriP21373.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

BioGridi33802. 62 interactions.
DIPiDIP-4479N.
IntActiP21373. 4 interactions.
MINTiMINT-549835.

Structurei

3D structure databases

ProteinModelPortaliP21373.
SMRiP21373. Positions 128-444.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.

Phylogenomic databases

eggNOGiCOG0061.
GeneTreeiENSGT00390000013792.
HOGENOMiHOG000176769.
OMAiIHNKLYC.
OrthoDBiEOG72VHFZ.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

P21373-1 [UniParc]FASTAAdd to Basket

« Hide

MKENDMNNGV DKWVNEEDGR NDHHNNNNNL MKKAMMNNEQ IDRTQDIDNA    50
KEMLRKISSE SSSRRSSLLN KDSSLVNGNA NSGGGTSING TRGSSKSSNT 100
HFQYASTAYG VRMLSKDISN TKVELDVENL MIVTKLNDVS LYFLTRELVE 150
WVLVHFPRVT VYVDSELKNS KKFAAGELCE DSKCRESRIK YWTKDFIREH 200
DVFFDLVVTL GGDGTVLFVS SIFQRHVPPV MSFSLGSLGF LTNFKFEHFR 250
EDLPRIMNHK IKTNLRLRLE CTIYRRHRPE VDPNTGKKIC VVEKLSTHHI 300
LNEVTIDRGP SPFLSMLELY GDGSLMTVAQ ADGLIAATPT GSTAYSLSAG 350
GSLVCPTVNA IALTPICPHA LSFRPIILPE SINLKVKVSM KSRAPAWAAF 400
DGKDRIELQK GDFITICASP YAFPTVEASP DEFINSISRQ LNWNVREQQK 450
SFTHILSQKN QEKYAHEANK VRNQAEPLEV IRDKYSLEAD ATKENNNGSD 500
DESDDESVNC EACKLKPSSV PKPSQARFSV 530
Length:530
Mass (Da):59,469
Last modified:February 1, 1995 - v2
Checksum:i1BF4F01D7213B94B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 3371A → V in AAT92918. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB044344 Genomic DNA. Translation: BAB83863.1.
L26347 Genomic DNA. Translation: AAA62857.1.
L36344 Genomic DNA. Translation: AAA88752.1.
M37696 Genomic DNA. No translation available.
Z49549 Genomic DNA. Translation: CAA89577.1.
AY692899 Genomic DNA. Translation: AAT92918.1.
BK006943 Genomic DNA. Translation: DAA08836.1.
PIRiS46589.
RefSeqiNP_012583.1. NM_001181707.1.

Genome annotation databases

EnsemblFungiiYJR049C; YJR049C; YJR049C.
GeneIDi853508.
KEGGisce:YJR049C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB044344 Genomic DNA. Translation: BAB83863.1 .
L26347 Genomic DNA. Translation: AAA62857.1 .
L36344 Genomic DNA. Translation: AAA88752.1 .
M37696 Genomic DNA. No translation available.
Z49549 Genomic DNA. Translation: CAA89577.1 .
AY692899 Genomic DNA. Translation: AAT92918.1 .
BK006943 Genomic DNA. Translation: DAA08836.1 .
PIRi S46589.
RefSeqi NP_012583.1. NM_001181707.1.

3D structure databases

ProteinModelPortali P21373.
SMRi P21373. Positions 128-444.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33802. 62 interactions.
DIPi DIP-4479N.
IntActi P21373. 4 interactions.
MINTi MINT-549835.

Proteomic databases

MaxQBi P21373.
PaxDbi P21373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR049C ; YJR049C ; YJR049C .
GeneIDi 853508.
KEGGi sce:YJR049C.

Organism-specific databases

CYGDi YJR049c.
SGDi S000003810. UTR1.

Phylogenomic databases

eggNOGi COG0061.
GeneTreei ENSGT00390000013792.
HOGENOMi HOG000176769.
OMAi IHNKLYC.
OrthoDBi EOG72VHFZ.

Enzyme and pathway databases

BioCyci MetaCyc:G3O-31684-MONOMER.
YEAST:G3O-31684-MONOMER.
BRENDAi 2.7.1.23. 984.

Miscellaneous databases

NextBioi 974165.

Gene expression databases

Genevestigatori P21373.

Family and domain databases

Gene3Di 2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPi MF_00361. NAD_kinase.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view ]
PANTHERi PTHR20275. PTHR20275. 1 hit.
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and identification of UTR1 of a yeast Saccharomyces cerevisiae as a gene encoding an NAD kinase."
    Kawai S., Mori S., Suzuki S., Murata K.
    FEMS Microbiol. Lett. 200:181-184(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  2. "Nucleotide sequence of the COR region: a cluster of six genes in the yeast Saccharomyces cerevisiae."
    Melnick L., Sherman F.
    Gene 87:157-166(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE.
  3. "Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 10:811-818(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499 AND SER-503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499 AND SER-503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUTR1_YEAST
AccessioniPrimary (citable) accession number: P21373
Secondary accession number(s): D6VWM0, E9P8Y8, Q54AD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5040 molecules/cell in log phase SD medium.

Caution

Was briefly thought to be FRE2.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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