Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD(+) kinase

Gene

UTR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically phosphorylates NAD in the presence of ATP, dATP, or CTP as phosphoryl donors.1 Publication

Miscellaneous

Present with 5040 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.

Kineticsi

  1. KM=0.50 mM for NAD1 Publication
  2. KM=0.60 mM for ATP1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • NAD+ kinase activity Source: SGD
    • NADH kinase activity Source: SGD

    GO - Biological processi

    • cellular iron ion homeostasis Source: SGD
    • NAD metabolic process Source: InterPro
    • NADP biosynthetic process Source: SGD

    Keywordsi

    Molecular functionKinase, Transferase
    LigandATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-31684-MONOMER.
    YEAST:G3O-31684-MONOMER.
    BRENDAi2.7.1.23. 984.
    ReactomeiR-SCE-196807. Nicotinate metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD(+) kinase (EC:2.7.1.23)
    Alternative name(s):
    Unknown transcript 1 protein
    Gene namesi
    Name:UTR1
    Ordered Locus Names:YJR049C
    ORF Names:J1655
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome X

    Organism-specific databases

    EuPathDBiFungiDB:YJR049C.
    SGDiS000003810. UTR1.

    Subcellular locationi

    GO - Cellular componenti

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001207161 – 530NAD(+) kinaseAdd BLAST530

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei499PhosphoserineCombined sources1
    Modified residuei503PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP21373.
    PRIDEiP21373.

    PTM databases

    iPTMnetiP21373.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Protein-protein interaction databases

    BioGridi33802. 84 interactors.
    DIPiDIP-4479N.
    IntActiP21373. 5 interactors.
    MINTiMINT-549835.
    STRINGi4932.YJR049C.

    Structurei

    3D structure databases

    ProteinModelPortaliP21373.
    SMRiP21373.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000013792.
    HOGENOMiHOG000176769.
    InParanoidiP21373.
    KOiK00858.
    OMAiIHNKLYC.
    OrthoDBiEOG092C2G28.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase. 1 hit.
    InterProiView protein in InterPro
    IPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiView protein in Pfam
    PF01513. NAD_kinase. 1 hit.
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P21373-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKENDMNNGV DKWVNEEDGR NDHHNNNNNL MKKAMMNNEQ IDRTQDIDNA
    60 70 80 90 100
    KEMLRKISSE SSSRRSSLLN KDSSLVNGNA NSGGGTSING TRGSSKSSNT
    110 120 130 140 150
    HFQYASTAYG VRMLSKDISN TKVELDVENL MIVTKLNDVS LYFLTRELVE
    160 170 180 190 200
    WVLVHFPRVT VYVDSELKNS KKFAAGELCE DSKCRESRIK YWTKDFIREH
    210 220 230 240 250
    DVFFDLVVTL GGDGTVLFVS SIFQRHVPPV MSFSLGSLGF LTNFKFEHFR
    260 270 280 290 300
    EDLPRIMNHK IKTNLRLRLE CTIYRRHRPE VDPNTGKKIC VVEKLSTHHI
    310 320 330 340 350
    LNEVTIDRGP SPFLSMLELY GDGSLMTVAQ ADGLIAATPT GSTAYSLSAG
    360 370 380 390 400
    GSLVCPTVNA IALTPICPHA LSFRPIILPE SINLKVKVSM KSRAPAWAAF
    410 420 430 440 450
    DGKDRIELQK GDFITICASP YAFPTVEASP DEFINSISRQ LNWNVREQQK
    460 470 480 490 500
    SFTHILSQKN QEKYAHEANK VRNQAEPLEV IRDKYSLEAD ATKENNNGSD
    510 520 530
    DESDDESVNC EACKLKPSSV PKPSQARFSV
    Length:530
    Mass (Da):59,469
    Last modified:February 1, 1995 - v2
    Checksum:i1BF4F01D7213B94B
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti337A → V in AAT92918 (PubMed:17322287).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB044344 Genomic DNA. Translation: BAB83863.1.
    L26347 Genomic DNA. Translation: AAA62857.1.
    L36344 Genomic DNA. Translation: AAA88752.1.
    M37696 Genomic DNA. No translation available.
    Z49549 Genomic DNA. Translation: CAA89577.1.
    AY692899 Genomic DNA. Translation: AAT92918.1.
    BK006943 Genomic DNA. Translation: DAA08836.1.
    PIRiS46589.
    RefSeqiNP_012583.1. NM_001181707.1.

    Genome annotation databases

    EnsemblFungiiYJR049C; YJR049C; YJR049C.
    GeneIDi853508.
    KEGGisce:YJR049C.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiUTR1_YEAST
    AccessioniPrimary (citable) accession number: P21373
    Secondary accession number(s): D6VWM0, E9P8Y8, Q54AD8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: February 1, 1995
    Last modified: July 5, 2017
    This is version 145 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    Was briefly thought to be FRE2.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names