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P21373 (UTR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD(+) kinase

EC=2.7.1.23
Alternative name(s):
Unknown transcript 1 protein
Gene names
Name:UTR1
Ordered Locus Names:YJR049C
ORF Names:J1655
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically phosphorylates NAD in the presence of ATP, dATP, or CTP as phosphoryl donors. Ref.1

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Subunit structure

Homohexamer. Ref.1

Miscellaneous

Present with 5040 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the NAD kinase family.

Caution

Was briefly thought to be FRE2.

Biophysicochemical properties

Kinetic parameters:

KM=0.50 mM for NAD Ref.1

KM=0.60 mM for ATP

pH dependence:

Optimum pH is 8.0.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 530530NAD(+) kinase HAMAP-Rule MF_00361
PRO_0000120716

Amino acid modifications

Modified residue4991Phosphoserine Ref.9 Ref.10
Modified residue5031Phosphoserine Ref.9 Ref.10

Experimental info

Sequence conflict3371A → V in AAT92918. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P21373 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 1BF4F01D7213B94B

FASTA53059,469
        10         20         30         40         50         60 
MKENDMNNGV DKWVNEEDGR NDHHNNNNNL MKKAMMNNEQ IDRTQDIDNA KEMLRKISSE 

        70         80         90        100        110        120 
SSSRRSSLLN KDSSLVNGNA NSGGGTSING TRGSSKSSNT HFQYASTAYG VRMLSKDISN 

       130        140        150        160        170        180 
TKVELDVENL MIVTKLNDVS LYFLTRELVE WVLVHFPRVT VYVDSELKNS KKFAAGELCE 

       190        200        210        220        230        240 
DSKCRESRIK YWTKDFIREH DVFFDLVVTL GGDGTVLFVS SIFQRHVPPV MSFSLGSLGF 

       250        260        270        280        290        300 
LTNFKFEHFR EDLPRIMNHK IKTNLRLRLE CTIYRRHRPE VDPNTGKKIC VVEKLSTHHI 

       310        320        330        340        350        360 
LNEVTIDRGP SPFLSMLELY GDGSLMTVAQ ADGLIAATPT GSTAYSLSAG GSLVCPTVNA 

       370        380        390        400        410        420 
IALTPICPHA LSFRPIILPE SINLKVKVSM KSRAPAWAAF DGKDRIELQK GDFITICASP 

       430        440        450        460        470        480 
YAFPTVEASP DEFINSISRQ LNWNVREQQK SFTHILSQKN QEKYAHEANK VRNQAEPLEV 

       490        500        510        520        530 
IRDKYSLEAD ATKENNNGSD DESDDESVNC EACKLKPSSV PKPSQARFSV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and identification of UTR1 of a yeast Saccharomyces cerevisiae as a gene encoding an NAD kinase."
Kawai S., Mori S., Suzuki S., Murata K.
FEMS Microbiol. Lett. 200:181-184(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
[2]"Nucleotide sequence of the COR region: a cluster of six genes in the yeast Saccharomyces cerevisiae."
Melnick L., Sherman F.
Gene 87:157-166(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE.
[3]"Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 10:811-818(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499 AND SER-503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499 AND SER-503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB044344 Genomic DNA. Translation: BAB83863.1.
L26347 Genomic DNA. Translation: AAA62857.1.
L36344 Genomic DNA. Translation: AAA88752.1.
M37696 Genomic DNA. No translation available.
Z49549 Genomic DNA. Translation: CAA89577.1.
AY692899 Genomic DNA. Translation: AAT92918.1.
BK006943 Genomic DNA. Translation: DAA08836.1.
PIRS46589.
RefSeqNP_012583.1. NM_001181707.1.

3D structure databases

ProteinModelPortalP21373.
SMRP21373. Positions 128-444.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33802. 62 interactions.
DIPDIP-4479N.
IntActP21373. 4 interactions.
MINTMINT-549835.

Proteomic databases

PaxDbP21373.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR049C; YJR049C; YJR049C.
GeneID853508.
KEGGsce:YJR049C.

Organism-specific databases

CYGDYJR049c.
SGDS000003810. UTR1.

Phylogenomic databases

eggNOGCOG0061.
GeneTreeENSGT00390000013792.
HOGENOMHOG000176769.
OMAIHNKLYC.
OrthoDBEOG72VHFZ.

Enzyme and pathway databases

BioCycMetaCyc:G3O-31684-MONOMER.
YEAST:G3O-31684-MONOMER.
BRENDA2.7.1.23. 984.

Gene expression databases

GenevestigatorP21373.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK_prd.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Other

NextBio974165.

Entry information

Entry nameUTR1_YEAST
AccessionPrimary (citable) accession number: P21373
Secondary accession number(s): D6VWM0, E9P8Y8, Q54AD8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1995
Last modified: March 19, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families