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Protein

NAD(+) kinase

Gene

UTR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically phosphorylates NAD in the presence of ATP, dATP, or CTP as phosphoryl donors.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.

Kineticsi

  1. KM=0.50 mM for NAD1 Publication
  2. KM=0.60 mM for ATP1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • NAD+ kinase activity Source: SGD
    • NADH kinase activity Source: SGD

    GO - Biological processi

    • cellular iron ion homeostasis Source: SGD
    • NAD metabolic process Source: InterPro
    • NADP biosynthetic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-31684-MONOMER.
    YEAST:G3O-31684-MONOMER.
    BRENDAi2.7.1.23. 984.
    ReactomeiREACT_352768. Nicotinate metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD(+) kinase (EC:2.7.1.23)
    Alternative name(s):
    Unknown transcript 1 protein
    Gene namesi
    Name:UTR1
    Ordered Locus Names:YJR049C
    ORF Names:J1655
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311 Componenti: Chromosome X

    Organism-specific databases

    CYGDiYJR049c.
    EuPathDBiFungiDB:YJR049C.
    SGDiS000003810. UTR1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 530530NAD(+) kinasePRO_0000120716Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei499 – 4991Phosphoserine2 Publications
    Modified residuei503 – 5031Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP21373.
    PaxDbiP21373.

    Expressioni

    Gene expression databases

    GenevestigatoriP21373.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Protein-protein interaction databases

    BioGridi33802. 62 interactions.
    DIPiDIP-4479N.
    IntActiP21373. 4 interactions.
    MINTiMINT-549835.

    Structurei

    3D structure databases

    ProteinModelPortaliP21373.
    SMRiP21373. Positions 128-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0061.
    GeneTreeiENSGT00390000013792.
    HOGENOMiHOG000176769.
    InParanoidiP21373.
    KOiK00858.
    OMAiIHNKLYC.
    OrthoDBiEOG72VHFZ.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P21373-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKENDMNNGV DKWVNEEDGR NDHHNNNNNL MKKAMMNNEQ IDRTQDIDNA
    60 70 80 90 100
    KEMLRKISSE SSSRRSSLLN KDSSLVNGNA NSGGGTSING TRGSSKSSNT
    110 120 130 140 150
    HFQYASTAYG VRMLSKDISN TKVELDVENL MIVTKLNDVS LYFLTRELVE
    160 170 180 190 200
    WVLVHFPRVT VYVDSELKNS KKFAAGELCE DSKCRESRIK YWTKDFIREH
    210 220 230 240 250
    DVFFDLVVTL GGDGTVLFVS SIFQRHVPPV MSFSLGSLGF LTNFKFEHFR
    260 270 280 290 300
    EDLPRIMNHK IKTNLRLRLE CTIYRRHRPE VDPNTGKKIC VVEKLSTHHI
    310 320 330 340 350
    LNEVTIDRGP SPFLSMLELY GDGSLMTVAQ ADGLIAATPT GSTAYSLSAG
    360 370 380 390 400
    GSLVCPTVNA IALTPICPHA LSFRPIILPE SINLKVKVSM KSRAPAWAAF
    410 420 430 440 450
    DGKDRIELQK GDFITICASP YAFPTVEASP DEFINSISRQ LNWNVREQQK
    460 470 480 490 500
    SFTHILSQKN QEKYAHEANK VRNQAEPLEV IRDKYSLEAD ATKENNNGSD
    510 520 530
    DESDDESVNC EACKLKPSSV PKPSQARFSV
    Length:530
    Mass (Da):59,469
    Last modified:February 1, 1995 - v2
    Checksum:i1BF4F01D7213B94B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti337 – 3371A → V in AAT92918 (PubMed:17322287).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB044344 Genomic DNA. Translation: BAB83863.1.
    L26347 Genomic DNA. Translation: AAA62857.1.
    L36344 Genomic DNA. Translation: AAA88752.1.
    M37696 Genomic DNA. No translation available.
    Z49549 Genomic DNA. Translation: CAA89577.1.
    AY692899 Genomic DNA. Translation: AAT92918.1.
    BK006943 Genomic DNA. Translation: DAA08836.1.
    PIRiS46589.
    RefSeqiNP_012583.1. NM_001181707.1.

    Genome annotation databases

    EnsemblFungiiYJR049C; YJR049C; YJR049C.
    GeneIDi853508.
    KEGGisce:YJR049C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB044344 Genomic DNA. Translation: BAB83863.1.
    L26347 Genomic DNA. Translation: AAA62857.1.
    L36344 Genomic DNA. Translation: AAA88752.1.
    M37696 Genomic DNA. No translation available.
    Z49549 Genomic DNA. Translation: CAA89577.1.
    AY692899 Genomic DNA. Translation: AAT92918.1.
    BK006943 Genomic DNA. Translation: DAA08836.1.
    PIRiS46589.
    RefSeqiNP_012583.1. NM_001181707.1.

    3D structure databases

    ProteinModelPortaliP21373.
    SMRiP21373. Positions 128-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi33802. 62 interactions.
    DIPiDIP-4479N.
    IntActiP21373. 4 interactions.
    MINTiMINT-549835.

    Proteomic databases

    MaxQBiP21373.
    PaxDbiP21373.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYJR049C; YJR049C; YJR049C.
    GeneIDi853508.
    KEGGisce:YJR049C.

    Organism-specific databases

    CYGDiYJR049c.
    EuPathDBiFungiDB:YJR049C.
    SGDiS000003810. UTR1.

    Phylogenomic databases

    eggNOGiCOG0061.
    GeneTreeiENSGT00390000013792.
    HOGENOMiHOG000176769.
    InParanoidiP21373.
    KOiK00858.
    OMAiIHNKLYC.
    OrthoDBiEOG72VHFZ.

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-31684-MONOMER.
    YEAST:G3O-31684-MONOMER.
    BRENDAi2.7.1.23. 984.
    ReactomeiREACT_352768. Nicotinate metabolism.

    Miscellaneous databases

    NextBioi974165.
    PROiP21373.

    Gene expression databases

    GenevestigatoriP21373.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Molecular cloning and identification of UTR1 of a yeast Saccharomyces cerevisiae as a gene encoding an NAD kinase."
      Kawai S., Mori S., Suzuki S., Murata K.
      FEMS Microbiol. Lett. 200:181-184(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    2. "Nucleotide sequence of the COR region: a cluster of six genes in the yeast Saccharomyces cerevisiae."
      Melnick L., Sherman F.
      Gene 87:157-166(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE.
    3. "Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X."
      Huang M.-E., Manus V., Chuat J.-C., Galibert F.
      Yeast 10:811-818(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499 AND SER-503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499 AND SER-503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUTR1_YEAST
    AccessioniPrimary (citable) accession number: P21373
    Secondary accession number(s): D6VWM0, E9P8Y8, Q54AD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: February 1, 1995
    Last modified: April 29, 2015
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5040 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was briefly thought to be FRE2.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.