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P21373

- UTR1_YEAST

UniProt

P21373 - UTR1_YEAST

Protein

NAD(+) kinase

Gene

UTR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Specifically phosphorylates NAD in the presence of ATP, dATP, or CTP as phosphoryl donors.1 Publication

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.

    Kineticsi

    1. KM=0.50 mM for NAD1 Publication
    2. KM=0.60 mM for ATP1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. NAD+ kinase activity Source: SGD
    3. NADH kinase activity Source: SGD

    GO - Biological processi

    1. cellular iron ion homeostasis Source: SGD
    2. NAD metabolic process Source: InterPro
    3. NADP biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-31684-MONOMER.
    YEAST:G3O-31684-MONOMER.
    BRENDAi2.7.1.23. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD(+) kinase (EC:2.7.1.23)
    Alternative name(s):
    Unknown transcript 1 protein
    Gene namesi
    Name:UTR1
    Ordered Locus Names:YJR049C
    ORF Names:J1655
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR049c.
    SGDiS000003810. UTR1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 530530NAD(+) kinasePRO_0000120716Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei499 – 4991Phosphoserine2 Publications
    Modified residuei503 – 5031Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP21373.
    PaxDbiP21373.

    Expressioni

    Gene expression databases

    GenevestigatoriP21373.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Protein-protein interaction databases

    BioGridi33802. 62 interactions.
    DIPiDIP-4479N.
    IntActiP21373. 4 interactions.
    MINTiMINT-549835.

    Structurei

    3D structure databases

    ProteinModelPortaliP21373.
    SMRiP21373. Positions 128-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0061.
    GeneTreeiENSGT00390000013792.
    HOGENOMiHOG000176769.
    OMAiIHNKLYC.
    OrthoDBiEOG72VHFZ.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P21373-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKENDMNNGV DKWVNEEDGR NDHHNNNNNL MKKAMMNNEQ IDRTQDIDNA    50
    KEMLRKISSE SSSRRSSLLN KDSSLVNGNA NSGGGTSING TRGSSKSSNT 100
    HFQYASTAYG VRMLSKDISN TKVELDVENL MIVTKLNDVS LYFLTRELVE 150
    WVLVHFPRVT VYVDSELKNS KKFAAGELCE DSKCRESRIK YWTKDFIREH 200
    DVFFDLVVTL GGDGTVLFVS SIFQRHVPPV MSFSLGSLGF LTNFKFEHFR 250
    EDLPRIMNHK IKTNLRLRLE CTIYRRHRPE VDPNTGKKIC VVEKLSTHHI 300
    LNEVTIDRGP SPFLSMLELY GDGSLMTVAQ ADGLIAATPT GSTAYSLSAG 350
    GSLVCPTVNA IALTPICPHA LSFRPIILPE SINLKVKVSM KSRAPAWAAF 400
    DGKDRIELQK GDFITICASP YAFPTVEASP DEFINSISRQ LNWNVREQQK 450
    SFTHILSQKN QEKYAHEANK VRNQAEPLEV IRDKYSLEAD ATKENNNGSD 500
    DESDDESVNC EACKLKPSSV PKPSQARFSV 530
    Length:530
    Mass (Da):59,469
    Last modified:February 1, 1995 - v2
    Checksum:i1BF4F01D7213B94B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti337 – 3371A → V in AAT92918. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB044344 Genomic DNA. Translation: BAB83863.1.
    L26347 Genomic DNA. Translation: AAA62857.1.
    L36344 Genomic DNA. Translation: AAA88752.1.
    M37696 Genomic DNA. No translation available.
    Z49549 Genomic DNA. Translation: CAA89577.1.
    AY692899 Genomic DNA. Translation: AAT92918.1.
    BK006943 Genomic DNA. Translation: DAA08836.1.
    PIRiS46589.
    RefSeqiNP_012583.1. NM_001181707.1.

    Genome annotation databases

    EnsemblFungiiYJR049C; YJR049C; YJR049C.
    GeneIDi853508.
    KEGGisce:YJR049C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB044344 Genomic DNA. Translation: BAB83863.1 .
    L26347 Genomic DNA. Translation: AAA62857.1 .
    L36344 Genomic DNA. Translation: AAA88752.1 .
    M37696 Genomic DNA. No translation available.
    Z49549 Genomic DNA. Translation: CAA89577.1 .
    AY692899 Genomic DNA. Translation: AAT92918.1 .
    BK006943 Genomic DNA. Translation: DAA08836.1 .
    PIRi S46589.
    RefSeqi NP_012583.1. NM_001181707.1.

    3D structure databases

    ProteinModelPortali P21373.
    SMRi P21373. Positions 128-444.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33802. 62 interactions.
    DIPi DIP-4479N.
    IntActi P21373. 4 interactions.
    MINTi MINT-549835.

    Proteomic databases

    MaxQBi P21373.
    PaxDbi P21373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR049C ; YJR049C ; YJR049C .
    GeneIDi 853508.
    KEGGi sce:YJR049C.

    Organism-specific databases

    CYGDi YJR049c.
    SGDi S000003810. UTR1.

    Phylogenomic databases

    eggNOGi COG0061.
    GeneTreei ENSGT00390000013792.
    HOGENOMi HOG000176769.
    OMAi IHNKLYC.
    OrthoDBi EOG72VHFZ.

    Enzyme and pathway databases

    BioCyci MetaCyc:G3O-31684-MONOMER.
    YEAST:G3O-31684-MONOMER.
    BRENDAi 2.7.1.23. 984.

    Miscellaneous databases

    NextBioi 974165.

    Gene expression databases

    Genevestigatori P21373.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and identification of UTR1 of a yeast Saccharomyces cerevisiae as a gene encoding an NAD kinase."
      Kawai S., Mori S., Suzuki S., Murata K.
      FEMS Microbiol. Lett. 200:181-184(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    2. "Nucleotide sequence of the COR region: a cluster of six genes in the yeast Saccharomyces cerevisiae."
      Melnick L., Sherman F.
      Gene 87:157-166(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE.
    3. "Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X."
      Huang M.-E., Manus V., Chuat J.-C., Galibert F.
      Yeast 10:811-818(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499 AND SER-503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499 AND SER-503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUTR1_YEAST
    AccessioniPrimary (citable) accession number: P21373
    Secondary accession number(s): D6VWM0, E9P8Y8, Q54AD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5040 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was briefly thought to be FRE2.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3