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Protein

Pre-mRNA-processing ATP-dependent RNA helicase PRP5

Gene

PRP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved spliceosome assembly and in nuclear splicing. Catalyzes an ATP-dependent conformational change of U2 snRNP. Bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA.8 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi300 – 307ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • mRNA branch site recognition Source: SGD
  • regulation of gene expression Source: GO_Central
  • RNA secondary structure unwinding Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29168-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing ATP-dependent RNA helicase PRP5 (EC:3.6.4.13)
Gene namesi
Name:PRP5
Synonyms:RNA5
Ordered Locus Names:YBR237W
ORF Names:YBR1603
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR237W.
SGDiS000000441. PRP5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi293G → D in PRP5-1; no growth at 37 degrees Celsius and impairs pre-spliceosome formation in vitro. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551261 – 849Pre-mRNA-processing ATP-dependent RNA helicase PRP5Add BLAST849

Proteomic databases

MaxQBiP21372.
PRIDEiP21372.

PTM databases

iPTMnetiP21372.

Interactioni

Subunit structurei

Interacts with the U2 snRNP and HSH155.2 Publications

Protein-protein interaction databases

BioGridi32932. 19 interactors.
DIPiDIP-88N.
IntActiP21372. 3 interactors.
MINTiMINT-403988.

Structurei

Secondary structure

1849
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi223 – 226Combined sources4
Helixi230 – 239Combined sources10
Beta strandi243 – 248Combined sources6
Helixi257 – 259Combined sources3
Helixi264 – 272Combined sources9
Helixi281 – 291Combined sources11
Beta strandi296 – 299Combined sources4
Helixi306 – 319Combined sources14
Beta strandi332 – 336Combined sources5
Helixi340 – 354Combined sources15
Beta strandi362 – 367Combined sources6
Helixi372 – 378Combined sources7
Beta strandi383 – 387Combined sources5
Helixi389 – 395Combined sources7
Helixi398 – 401Combined sources4
Beta strandi411 – 414Combined sources4
Helixi417 – 422Combined sources6
Helixi426 – 435Combined sources10
Beta strandi441 – 447Combined sources7
Helixi451 – 460Combined sources10
Beta strandi461 – 463Combined sources3
Beta strandi465 – 472Combined sources8
Beta strandi479 – 488Combined sources10
Helixi489 – 510Combined sources22
Beta strandi528 – 533Combined sources6
Helixi535 – 547Combined sources13
Beta strandi553 – 555Combined sources3
Helixi561 – 573Combined sources13
Beta strandi578 – 582Combined sources5
Helixi583 – 586Combined sources4
Beta strandi595 – 601Combined sources7
Helixi606 – 613Combined sources8
Helixi614 – 616Combined sources3
Helixi619 – 621Combined sources3
Beta strandi624 – 630Combined sources7
Helixi634 – 643Combined sources10
Helixi646 – 650Combined sources5
Helixi654 – 672Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LJYX-ray1.95A206-698[»]
4LK2X-ray2.12A/B206-698[»]
ProteinModelPortaliP21372.
SMRiP21372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini287 – 467Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST181
Domaini502 – 661Helicase C-terminalPROSITE-ProRule annotationAdd BLAST160

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili13 – 81Sequence analysisAdd BLAST69

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi90 – 96Nuclear localization signalSequence analysis7
Motifi255 – 284Q motifAdd BLAST30
Motifi415 – 418DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00840000129934.
HOGENOMiHOG000007229.
InParanoidiP21372.
KOiK12811.
OMAiQCVLFSA.
OrthoDBiEOG092C1B4A.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004088. KH_dom_type_1.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METIDSKQNI NRESLLEERR KKLAKWKQKK AQFDAQKEHQ TSRNDIVTNS
60 70 80 90 100
LEGKQTTEKF TERQERVKEE LRKRKNEFRK SDEPVSVKPS KKKSKRSKVK
110 120 130 140 150
KKISFDFSDD DDSEIGVSFR SKEHIQKAPE HDNEKDPLDE FMTSLKEEKM
160 170 180 190 200
SNSKGMYDRG DILDVEDQLF ELGGTDDEDV EDNTDNSNIA KIAKLKAKKR
210 220 230 240 250
VKQIYYSPEE LEPFQKNFYI ESETVSSMSE MEVEELRLSL DNIKIKGTGC
260 270 280 290 300
PKPVTKWSQL GLSTDTMVLI TEKLHFGSLT PIQSQALPAI MSGRDVIGIS
310 320 330 340 350
KTGSGKTISY LLPLLRQVKA QRPLSKHETG PMGLILAPTR ELALQIHEEV
360 370 380 390 400
TKFTEADTSI RSVCCTGGSE MKKQITDLKR GTEIVVATPG RFIDILTLND
410 420 430 440 450
GKLLSTKRIT FVVMDEADRL FDLGFEPQIT QIMKTVRPDK QCVLFSATFP
460 470 480 490 500
NKLRSFAVRV LHSPISITIN SKGMVNENVK QKFRICHSED EKFDNLVQLI
510 520 530 540 550
HERSEFFDEV QSENDGQSSD VEEVDAKAII FVSSQNICDF ISKKLLNAGI
560 570 580 590 600
VTCAIHAGKP YQERLMNLEK FKREKNSILL CTEVLSRGLN VPEVSLVIIY
610 620 630 640 650
NAVKTFAQYV HTTGRTARGS RSGTAITLLL HDELSGAYIL SKAMRDEEIK
660 670 680 690 700
ALDPLQAKEL QEMSAKFESG MKKGKFRLSK GFGGKGLENI KSKREEAQNK
710 720 730 740 750
DLELKKNDKR SDDLEKKISN PREGHDSVSE SSALIPRLNY ELFKESTDGS
760 770 780 790 800
IIFYAKVYIN DLPQIVRWEA TKNTTLLFIK HETGCSITNK GKFYPEGKEP
810 820 830 840
KNENDEPKLY LLIEGQDEKD IQLSIELLEQ KVKEGVVKAA SLSLKSTKY
Length:849
Mass (Da):96,359
Last modified:May 1, 1991 - v1
Checksum:i326AAB3473ED7423
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33191 Genomic DNA. Translation: AAA34914.1.
Z36106 Genomic DNA. Translation: CAA85200.1.
AY692817 Genomic DNA. Translation: AAT92836.1.
BK006936 Genomic DNA. Translation: DAA07353.1.
PIRiA35791.
RefSeqiNP_009796.1. NM_001178585.1.

Genome annotation databases

EnsemblFungiiYBR237W; YBR237W; YBR237W.
GeneIDi852539.
KEGGisce:YBR237W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33191 Genomic DNA. Translation: AAA34914.1.
Z36106 Genomic DNA. Translation: CAA85200.1.
AY692817 Genomic DNA. Translation: AAT92836.1.
BK006936 Genomic DNA. Translation: DAA07353.1.
PIRiA35791.
RefSeqiNP_009796.1. NM_001178585.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LJYX-ray1.95A206-698[»]
4LK2X-ray2.12A/B206-698[»]
ProteinModelPortaliP21372.
SMRiP21372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32932. 19 interactors.
DIPiDIP-88N.
IntActiP21372. 3 interactors.
MINTiMINT-403988.

PTM databases

iPTMnetiP21372.

Proteomic databases

MaxQBiP21372.
PRIDEiP21372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR237W; YBR237W; YBR237W.
GeneIDi852539.
KEGGisce:YBR237W.

Organism-specific databases

EuPathDBiFungiDB:YBR237W.
SGDiS000000441. PRP5.

Phylogenomic databases

GeneTreeiENSGT00840000129934.
HOGENOMiHOG000007229.
InParanoidiP21372.
KOiK12811.
OMAiQCVLFSA.
OrthoDBiEOG092C1B4A.

Enzyme and pathway databases

BioCyciYEAST:G3O-29168-MONOMER.

Miscellaneous databases

PROiP21372.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004088. KH_dom_type_1.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP5_YEAST
AccessioniPrimary (citable) accession number: P21372
Secondary accession number(s): D6VQN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.