Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyrazinamidase/nicotinamidase

Gene

pncA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Nicotinamide + H2O = nicotinate + NH3.

Pathwayi: nicotinate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate from nicotinamide.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyrazinamidase/nicotinamidase (pncA)
This subpathway is part of the pathway nicotinate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate from nicotinamide, the pathway nicotinate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei10 – 101Sequence analysis
Metal bindingi52 – 521ZincBy similarity
Metal bindingi54 – 541ZincBy similarity
Metal bindingi86 – 861ZincBy similarity
Active sitei111 – 1111Sequence analysis
Active sitei156 – 1561NucleophileSequence analysis

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • nicotinamidase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:NICOTINAMID-MONOMER.
ECOL316407:JW1757-MONOMER.
MetaCyc:NICOTINAMID-MONOMER.
RETL1328306-WGS:GSTH-2863-MONOMER.
UniPathwayiUPA00830; UER00790.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrazinamidase/nicotinamidase (EC:3.5.1.-, EC:3.5.1.19)
Short name:
PZAase
Alternative name(s):
Nicotine deamidase
Short name:
NAMase
Gene namesi
Name:pncA
Synonyms:nam, ydjB
Ordered Locus Names:b1768, JW1757
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11135. pncA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Pyrazinamidase/nicotinamidasePRO_0000206557Add
BLAST

Proteomic databases

EPDiP21369.
PaxDbiP21369.
PRIDEiP21369.

Interactioni

Protein-protein interaction databases

BioGridi4262963. 17 interactions.
DIPiDIP-10520N.
IntActiP21369. 4 interactions.
STRINGi511145.b1768.

Structurei

3D structure databases

ProteinModelPortaliP21369.
SMRiP21369. Positions 2-199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PncA family.Curated

Phylogenomic databases

eggNOGiENOG4108RWI. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078666.
InParanoidiP21369.
KOiK08281.
OMAiDSWPPHC.
OrthoDBiEOG615VP6.
PhylomeDBiP21369.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
InterProiIPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.

Sequencei

Sequence statusi: Complete.

P21369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRALLLVD LQNDFCAGGA LAVPEGDSTV DVANRLIDWC QSRGEAVIAS
60 70 80 90 100
QDWHPANHGS FASQHGVEPY TPGQLDGLPQ TFWPDHCVQN SEGAQLHPLL
110 120 130 140 150
HQKAIAAVFH KGENPLVDSY SAFFDNGRRQ KTSLDDWLRD HEIDELIVMG
160 170 180 190 200
LATDYCVKFT VLDALQLGYK VNVITDGCRG VNIQPQDSAH AFMEMSAAGA
210
TLYTLADWEE TQG
Length:213
Mass (Da):23,362
Last modified:May 1, 1991 - v1
Checksum:iB9F8D946FA18433F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26934 Genomic DNA. Translation: AAA23447.1.
U00096 Genomic DNA. Translation: AAC74838.2.
AP009048 Genomic DNA. Translation: BAA15559.1.
PIRiH64936. QQECA5.
RefSeqiNP_416282.4. NC_000913.3.
WP_001135066.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74838; AAC74838; b1768.
BAA15559; BAA15559; BAA15559.
GeneIDi946276.
KEGGiecj:JW1757.
eco:b1768.
PATRICi32118847. VBIEscCol129921_1841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26934 Genomic DNA. Translation: AAA23447.1.
U00096 Genomic DNA. Translation: AAC74838.2.
AP009048 Genomic DNA. Translation: BAA15559.1.
PIRiH64936. QQECA5.
RefSeqiNP_416282.4. NC_000913.3.
WP_001135066.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP21369.
SMRiP21369. Positions 2-199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262963. 17 interactions.
DIPiDIP-10520N.
IntActiP21369. 4 interactions.
STRINGi511145.b1768.

Proteomic databases

EPDiP21369.
PaxDbiP21369.
PRIDEiP21369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74838; AAC74838; b1768.
BAA15559; BAA15559; BAA15559.
GeneIDi946276.
KEGGiecj:JW1757.
eco:b1768.
PATRICi32118847. VBIEscCol129921_1841.

Organism-specific databases

EchoBASEiEB1125.
EcoGeneiEG11135. pncA.

Phylogenomic databases

eggNOGiENOG4108RWI. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078666.
InParanoidiP21369.
KOiK08281.
OMAiDSWPPHC.
OrthoDBiEOG615VP6.
PhylomeDBiP21369.

Enzyme and pathway databases

UniPathwayiUPA00830; UER00790.
BioCyciEcoCyc:NICOTINAMID-MONOMER.
ECOL316407:JW1757-MONOMER.
MetaCyc:NICOTINAMID-MONOMER.
RETL1328306-WGS:GSTH-2863-MONOMER.

Miscellaneous databases

PROiP21369.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
InterProiIPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression in Escherichia coli K-12 of the L-asparaginase I-encoding ansA gene and its flanking regions."
    Jerlstroem P.G., Bezjak D.A., Jennings M.P., Beacham I.R.
    Gene 78:37-46(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA."
    Frothingham R., Meeker-O'Connell W.A., Talbot E.A., George J.W., Kreuzer K.N.
    Antimicrob. Agents Chemother. 40:1426-1431(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPNCA_ECOLI
AccessioniPrimary (citable) accession number: P21369
Secondary accession number(s): P76229, P76910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: March 16, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.