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Protein

Nicotinamidase

Gene

pncA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the deamidation of nicotinamide (NAM) into nicotinate (PubMed:4399474, PubMed:8726014). Likely functions in the cyclical salvage pathway for production of NAD from nicotinamide (PubMed:4399474).1 Publication1 Publication
Is also able to hydrolyze the first-line antituberculous drug pyrazinamide (PZA) into pyrazinoic acid in vitro, but this reaction is not considered to be physiologically relevant.1 Publication

Catalytic activityi

Nicotinamide + H2O = nicotinate + NH3.1 Publication1 Publication
Pyrazinamide + H2O = pyrazine-2-carboxylate + NH3.1 Publication

Kineticsi

  1. KM=70 µM for nicotinamide1 Publication
  1. Vmax=560 µmol/min/mg enzyme for the nicotinamidase activity1 Publication

pH dependencei

Optimum pH is 7.2.1 Publication

Pathwayi: nicotinate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate from nicotinamide.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinamidase (pncA)
This subpathway is part of the pathway nicotinate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate from nicotinamide, the pathway nicotinate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei10Proton acceptorBy similarity1
Metal bindingi52ZincBy similarity1
Metal bindingi54ZincBy similarity1
Metal bindingi86ZincBy similarity1
Active sitei111By similarity1
Active sitei156NucleophileBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • nicotinamidase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processPyridine nucleotide biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:NICOTINAMID-MONOMER.
MetaCyc:NICOTINAMID-MONOMER.
UniPathwayiUPA00830; UER00790.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamidase2 Publications (EC:3.5.1.191 Publication1 Publication)
Alternative name(s):
Nicotinamide deamidase1 Publication
Short name:
NAMase1 Publication
Pyrazinamidase1 Publication (EC:3.5.1.-1 Publication)
Short name:
PZAase1 Publication
Gene namesi
Name:pncA1 Publication
Synonyms:nam, ydjB
Ordered Locus Names:b1768, JW1757
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11135. pncA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002065571 – 213NicotinamidaseAdd BLAST213

Proteomic databases

EPDiP21369.
PaxDbiP21369.
PRIDEiP21369.

Expressioni

Inductioni

Is constitutively expressed in very small amounts in E.coli.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262963. 17 interactors.
DIPiDIP-10520N.
IntActiP21369. 4 interactors.
STRINGi511145.b1768.

Structurei

3D structure databases

ProteinModelPortaliP21369.
SMRiP21369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family.Curated

Phylogenomic databases

eggNOGiENOG4108RWI. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078666.
InParanoidiP21369.
KOiK08281.
OMAiDSWPPHC.
PhylomeDBiP21369.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
InterProiView protein in InterPro
IPR000868. Isochorismatase-like.
PfamiView protein in Pfam
PF00857. Isochorismatase. 1 hit.
SUPFAMiSSF52499. SSF52499. 1 hit.

Sequencei

Sequence statusi: Complete.

P21369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRALLLVD LQNDFCAGGA LAVPEGDSTV DVANRLIDWC QSRGEAVIAS
60 70 80 90 100
QDWHPANHGS FASQHGVEPY TPGQLDGLPQ TFWPDHCVQN SEGAQLHPLL
110 120 130 140 150
HQKAIAAVFH KGENPLVDSY SAFFDNGRRQ KTSLDDWLRD HEIDELIVMG
160 170 180 190 200
LATDYCVKFT VLDALQLGYK VNVITDGCRG VNIQPQDSAH AFMEMSAAGA
210
TLYTLADWEE TQG
Length:213
Mass (Da):23,362
Last modified:May 1, 1991 - v1
Checksum:iB9F8D946FA18433F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26934 Genomic DNA. Translation: AAA23447.1.
U00096 Genomic DNA. Translation: AAC74838.2.
AP009048 Genomic DNA. Translation: BAA15559.1.
PIRiH64936. QQECA5.
RefSeqiNP_416282.4. NC_000913.3.
WP_001135066.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74838; AAC74838; b1768.
BAA15559; BAA15559; BAA15559.
GeneIDi946276.
KEGGiecj:JW1757.
eco:b1768.
PATRICi32118847. VBIEscCol129921_1841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26934 Genomic DNA. Translation: AAA23447.1.
U00096 Genomic DNA. Translation: AAC74838.2.
AP009048 Genomic DNA. Translation: BAA15559.1.
PIRiH64936. QQECA5.
RefSeqiNP_416282.4. NC_000913.3.
WP_001135066.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP21369.
SMRiP21369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262963. 17 interactors.
DIPiDIP-10520N.
IntActiP21369. 4 interactors.
STRINGi511145.b1768.

Proteomic databases

EPDiP21369.
PaxDbiP21369.
PRIDEiP21369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74838; AAC74838; b1768.
BAA15559; BAA15559; BAA15559.
GeneIDi946276.
KEGGiecj:JW1757.
eco:b1768.
PATRICi32118847. VBIEscCol129921_1841.

Organism-specific databases

EchoBASEiEB1125.
EcoGeneiEG11135. pncA.

Phylogenomic databases

eggNOGiENOG4108RWI. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078666.
InParanoidiP21369.
KOiK08281.
OMAiDSWPPHC.
PhylomeDBiP21369.

Enzyme and pathway databases

UniPathwayiUPA00830; UER00790.
BioCyciEcoCyc:NICOTINAMID-MONOMER.
MetaCyc:NICOTINAMID-MONOMER.

Miscellaneous databases

PROiP21369.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
InterProiView protein in InterPro
IPR000868. Isochorismatase-like.
PfamiView protein in Pfam
PF00857. Isochorismatase. 1 hit.
SUPFAMiSSF52499. SSF52499. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNCA_ECOLI
AccessioniPrimary (citable) accession number: P21369
Secondary accession number(s): P76229, P76910
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: March 15, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.