Reviewed, UniProtKB/Swiss-Prot P21360 (MASY_PICAN)

Names and origin

Protein names

Recommended name:
    Malate synthase, glyoxysomal
    EC=2.3.3.9

Gene names

Name:

MAS

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

4905 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Glyoxysome.
Induction	By ethanol.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Malate synthase, glyoxysomal		PRO_0000166863
Sites
	Active site	179	1	Proton acceptor By similarity
	Active site	460	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21360-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 88137B77B2ECE303	FASTA	555	63,255
	10 20 30 40 50 60 MAQINPTTLD NVKILGEVSD KPLLSKATPR DILTKDALKF IVLLHRSFNE TRKQLLENRQ 70 80 90 100 110 120 KVQERLDAGE SLHFLEETKY IREDPNWKCL PTHPKLQCRK IEITGPPDAK MIVNAFNTDV 130 140 150 160 170 180 FTYMTDFEDS CSPTWNNMIY GQVNLYDAIR DRIDFTNEAT GKRYKINREG RRVPVMIVRP 190 200 210 220 230 240 RGWHMVDKHI LVDGEPISAS IMDFGLFFFH NAKFLLSQGL APFFYLPKME HYKEAQLWND 250 260 270 280 290 300 IFCVAQDCLE IPRGTIKATV LIETLPISYQ LDEVLYALRD HSAGLNCGRW DYMFSTIKRL 310 320 330 340 350 360 RNQKQHILPD RHQVTMTVPF MTNYVKQLIK ICHKRGVHAM GGMAATIPIK DDPEKNAAAM 370 380 390 400 410 420 EAVRQDKLRE VLAGHDGTWI AHPGLLPTAL SVFQEHMPTP NQIHVQKNVE ITEADLVDTN 430 440 450 460 470 480 IPDGKITMKG VSANIYIGLN YMESWLRGLG CVPINNLMED AATAEVSRLQ LYSWCKHAVK 490 500 510 520 530 540 MDDTGKTITP EFISKLIDEE AERCAANKPN NKFKIAADCL KKEINGHTEV AEFLTTLLYD 550 DIVTIGPEVD ISSLK

References

[1]	"Cloning and sequencing of the malate synthase gene from Hansenula polymorpha." Bruinenberg P.G., Blaauw M., Kazemier B., Ab G. Yeast 6:245-254(1990) [PubMed: 2349836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 234-253. Strain: MAYA CBS4732.

Cross-references

Sequence databases
PIR	SYHQMA. S09294.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.3.9. 276392.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_PICAN

Accession

Primary (citable) accession number: P21360

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	October 13, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)