Reviewed, UniProtKB/Swiss-Prot P21357 (AROF_SOLTU)

Names and origin

Protein names

Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase 1
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 1
    DAHP synthase 1

Gene names

Name:	SHKA
Synonyms:	ARO1

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Enzyme regulation	Activation by tryptophan (a hysteretic factor).
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subcellular location	Plastid › chloroplast.
Induction	By the herbicide glyphosate and wounding.
Sequence similarities	Belongs to the class-II DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 74	74	Chloroplast
	Chain	75 – 538	464	Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic		PRO_0000002302
Amino acid modifications
	Modified residue	75	1	Blocked amino end (Thr)

Sequences

	Sequence		Length	Mass (Da)
	P21357-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 9088EDF97CA14B13	FASTA	538	59,668
	10 20 30 40 50 60 MALSSTSTTN SLLPNRSLVQ NQPLLPSPLK NAFFSNNSTK TVRFVQPISA VHSSDSNKIP 70 80 90 100 110 120 IVSDKPSKSS PPAATATTAP APAVTKTEWA VDSWKSKKAL QLPEYPNQEE LRSVLKTIDE 130 140 150 160 170 180 FPPIVFAGEA RSLEERLGEA AMGRAFLLQG GDCAESFKEF NANNIRDTFR ILLQMGAVLM 190 200 210 220 230 240 FGGQMPVIKV GRMAGQFAKP RSDSFEEKDG VKLPSYRGDN VNGDAFDVKS RTPDPQRLIR 250 260 270 280 290 300 AYCQSAATLN LLRAFATGGY AAMQRINQWN LDFTEHSEQG DRYRELASRV DEALGFMTAA 310 320 330 340 350 360 GLTMDHPIMK TTEFWTSHEC LLLPYEQSLT RRDSTSGLYY DCSAHFLWVG ERTRQLDGAH 370 380 390 400 410 420 VEFLRGIANP LGIKVSDKMD PSALVKLIEI LNPQNKAGRI TIITRMGAEN MRVKLPHLIR 430 440 450 460 470 480 AVRRAGQIVT WVSDPMHGNT IKAPCGLKTR PFDSIRAEVR AFFDVHDQEG SHPGGVHLEM 490 500 510 520 530 TGQNVTECIG GSRTVTFDDL SSRYHTHCDP RLNASQSLEL SFIIAERLRK RRLGSQSV

References

[1]

"A cDNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Solanum tuberosum L." Dyer W.E., Weaver L.M., Zhao J., Kuhn D.N., Weller S.C., Herrmann K.M. J. Biol. Chem. 265:1608-1614(1990) [PubMed: 1967256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Superior.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05191 mRNA. Translation: AAA33810.1. Sequence problems.
PIR	A35016.
3D structure databases
SMR	P21357. Positions 87-530.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.54. 296.
Family and domain databases
InterPro	IPR002480. DAHP_synth_2. [Graphical view]
PANTHER	PTHR21337. DAHP_synth_2. 1 hit.
Pfam	PF01474. DAHP_synth_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01358. DAHP_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROF_SOLTU

Accession

Primary (citable) accession number: P21357

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)