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Protein

Proton/glutamate-aspartate symporter

Gene

gltP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the proton-dependent, binding-protein-independent transport of glutamate and aspartate.4 Publications

Enzyme regulationi

Glutamate uptake is inhibited by L-cysteate and beta-hydroxyaspartate. Inhibited by the uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP).3 Publications

Kineticsi

    1. Vmax=2.6 nmol/min/mg enzyme with glutamate as substrate1 Publication
    2. Vmax=2.3 nmol/min/mg enzyme with aspartate as substrate1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • aspartate transport Source: InterPro
    • dicarboxylic acid transport Source: EcoCyc
    • L-glutamate transport Source: InterPro
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Symport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:GLTP-MONOMER.
    ECOL316407:JW4038-MONOMER.
    MetaCyc:GLTP-MONOMER.

    Protein family/group databases

    TCDBi2.A.23.1.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proton/glutamate-aspartate symporterUniRule annotationCurated
    Alternative name(s):
    Glutamate-aspartate carrier proteinCurated
    Proton-glutamate-aspartate transport protein1 Publication
    Gene namesi
    Name:gltP2 PublicationsUniRule annotation
    Ordered Locus Names:b4077, JW4038
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10405. gltP.

    Subcellular locationi

    • Cell inner membrane UniRule annotation3 Publications; Multi-pass membrane protein UniRule annotation

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 5CytoplasmicCurated5
    Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
    Topological domaini27 – 50PeriplasmicCuratedAdd BLAST24
    Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
    Topological domaini72 – 84CytoplasmicCuratedAdd BLAST13
    Transmembranei85 – 105HelicalSequence analysisAdd BLAST21
    Topological domaini106 – 159PeriplasmicCuratedAdd BLAST54
    Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
    Topological domaini181 – 210CytoplasmicCuratedAdd BLAST30
    Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
    Topological domaini232PeriplasmicCurated1
    Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
    Topological domaini254 – 292CytoplasmicCuratedAdd BLAST39
    Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
    Topological domaini314 – 324PeriplasmicCuratedAdd BLAST11
    Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
    Topological domaini346 – 361CytoplasmicCuratedAdd BLAST16
    Transmembranei362 – 382HelicalSequence analysisAdd BLAST21
    Topological domaini383 – 387PeriplasmicCurated5
    Transmembranei388 – 408HelicalSequence analysisAdd BLAST21
    Topological domaini409 – 437Cytoplasmic1 PublicationAdd BLAST29

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002020881 – 437Proton/glutamate-aspartate symporterAdd BLAST437

    Proteomic databases

    PaxDbiP21345.
    PRIDEiP21345.

    Interactioni

    Protein-protein interaction databases

    BioGridi4262679. 6 interactors.
    STRINGi511145.b4077.

    Structurei

    3D structure databases

    ProteinModelPortaliP21345.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. GltP subfamily. [View classification]UniRule annotationCurated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105CK2. Bacteria.
    COG1301. LUCA.
    HOGENOMiHOG000208778.
    InParanoidiP21345.
    KOiK11102.
    OMAiICSFVVP.
    PhylomeDBiP21345.

    Family and domain databases

    Gene3Di1.10.3860.10. 1 hit.
    HAMAPiMF_02063. GltP_subfam. 1 hit.
    InterProiIPR033380. GltP/GltT.
    IPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11958. PTHR11958. 1 hit.
    PTHR11958:SF26. PTHR11958:SF26. 1 hit.
    PfamiPF00375. SDF. 1 hit.
    [Graphical view]
    PRINTSiPR00173. EDTRNSPORT.
    SUPFAMiSSF118215. SSF118215. 1 hit.
    PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21345-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKNIKFSLAW QILFAMVLGI LLGSYLHYHS DSRDWLVVNL LSPAGDIFIH
    60 70 80 90 100
    LIKMIVVPIV ISTLVVGIAG VGDAKQLGRI GAKTIIYFEV ITTVAIILGI
    110 120 130 140 150
    TLANVFQPGA GVDMSQLATV DISKYQSTTE AVQSSSHGIM GTILSLVPTN
    160 170 180 190 200
    IVASMAKGEM LPIIFFSVLF GLGLSSLPAT HREPLVTVFR SISETMFKVT
    210 220 230 240 250
    HMVMRYAPVG VFALIAVTVA NFGFSSLWPL AKLVLLVHFA ILFFALVVLG
    260 270 280 290 300
    IVARLCGLSV WILIRILKDE LILAYSTASS ESVLPRIIEK MEAYGAPVSI
    310 320 330 340 350
    TSFVVPTGYS FNLDGSTLYQ SIAAIFIAQL YGIDLSIWQE IILVLTLMVT
    360 370 380 390 400
    SKGIAGVPGV SFVVLLATLG SVGIPLEGLA FIAGVDRILD MARTALNVVG
    410 420 430
    NALAVLVIAK WEHKFDRKKA LAYEREVLGK FDKTADQ
    Length:437
    Mass (Da):47,159
    Last modified:March 1, 1992 - v2
    Checksum:i62E1A5FC16EA54BE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M32488 Genomic DNA. Translation: AAA23832.1. Sequence problems.
    M84805 Genomic DNA. Translation: AAA24323.1.
    U00006 Genomic DNA. Translation: AAC43171.1.
    U00096 Genomic DNA. Translation: AAD13460.1.
    AP009048 Genomic DNA. Translation: BAE78079.1.
    PIRiA42384.
    RefSeqiNP_418501.1. NC_000913.3.
    WP_000789592.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAD13460; AAD13460; b4077.
    BAE78079; BAE78079; BAE78079.
    GeneIDi948591.
    KEGGiecj:JW4038.
    eco:b4077.
    PATRICi32123705. VBIEscCol129921_4201.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M32488 Genomic DNA. Translation: AAA23832.1. Sequence problems.
    M84805 Genomic DNA. Translation: AAA24323.1.
    U00006 Genomic DNA. Translation: AAC43171.1.
    U00096 Genomic DNA. Translation: AAD13460.1.
    AP009048 Genomic DNA. Translation: BAE78079.1.
    PIRiA42384.
    RefSeqiNP_418501.1. NC_000913.3.
    WP_000789592.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP21345.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262679. 6 interactors.
    STRINGi511145.b4077.

    Protein family/group databases

    TCDBi2.A.23.1.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

    Proteomic databases

    PaxDbiP21345.
    PRIDEiP21345.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD13460; AAD13460; b4077.
    BAE78079; BAE78079; BAE78079.
    GeneIDi948591.
    KEGGiecj:JW4038.
    eco:b4077.
    PATRICi32123705. VBIEscCol129921_4201.

    Organism-specific databases

    EchoBASEiEB0400.
    EcoGeneiEG10405. gltP.

    Phylogenomic databases

    eggNOGiENOG4105CK2. Bacteria.
    COG1301. LUCA.
    HOGENOMiHOG000208778.
    InParanoidiP21345.
    KOiK11102.
    OMAiICSFVVP.
    PhylomeDBiP21345.

    Enzyme and pathway databases

    BioCyciEcoCyc:GLTP-MONOMER.
    ECOL316407:JW4038-MONOMER.
    MetaCyc:GLTP-MONOMER.

    Miscellaneous databases

    PROiP21345.

    Family and domain databases

    Gene3Di1.10.3860.10. 1 hit.
    HAMAPiMF_02063. GltP_subfam. 1 hit.
    InterProiIPR033380. GltP/GltT.
    IPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11958. PTHR11958. 1 hit.
    PTHR11958:SF26. PTHR11958:SF26. 1 hit.
    PfamiPF00375. SDF. 1 hit.
    [Graphical view]
    PRINTSiPR00173. EDTRNSPORT.
    SUPFAMiSSF118215. SSF118215. 1 hit.
    PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLTP_ECOLI
    AccessioniPrimary (citable) accession number: P21345
    Secondary accession number(s): Q2M6M7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: March 1, 1992
    Last modified: November 2, 2016
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.