Proton glutamate symport protein - Escherichia coli (strain K12)

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P21345 (GLTP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Proton glutamate symport protein

Alternative name(s):
Glutamate-aspartate carrier protein

Gene names

Name:	gltP
Ordered Locus Names:	b4077, JW4038

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This carrier protein is part of the Na⁺-independent, binding-protein-independent glutamate-aspartate transport system.
Enzyme regulation	Glutamate uptake was inhibited by L-cysteate and DL-3-hydroxyaspartate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.1. Source: EcoCyc
Molecular_function	sodium:dicarboxylate symporter activity Inferred from direct assay PubMed 336628. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 437

437

Proton glutamate symport protein

PRO_0000202088

Regions

Topological domain

1 – 23

Cytoplasmic Potential

Transmembrane

24 – 46

Helical; Name=1; Potential

Topological domain

47 – 65

Periplasmic Potential

Transmembrane

66 – 84

Helical; Name=2; Potential

Topological domain

85 – 96

Cytoplasmic Potential

Transmembrane

97 – 115

Helical; Name=3; Potential

Topological domain

116 – 149

Periplasmic Potential

Transmembrane

150 – 168

Helical; Name=4; Potential

Topological domain

169 – 171

Cytoplasmic Potential

Transmembrane

172 – 191

Helical; Name=5; Potential

Topological domain

192 – 210

Periplasmic Potential

Transmembrane

211 – 229

Helical; Name=6; Potential

Topological domain

230 – 242

Cytoplasmic Potential

Transmembrane

243 – 261

Helical; Name=7; Potential

Topological domain

262 – 282

Periplasmic Potential

Transmembrane

283 – 301

Helical; Name=8; Potential

Topological domain

302 – 321

Cytoplasmic Potential

Transmembrane

322 – 340

Helical; Name=9; Potential

Topological domain

341 – 351

Periplasmic Potential

Transmembrane

352 – 370

Helical; Name=10; Potential

Topological domain

371 – 378

Cytoplasmic Potential

Transmembrane

379 – 398

Helical; Name=11; Potential

Topological domain

399 – 405

Periplasmic Potential

Transmembrane

406 – 419

Helical; Name=12; Potential

Topological domain

420 – 437

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P21345 [UniParc].

Last modified March 1, 1992. Version 2.
Checksum: 62E1A5FC16EA54BE
Show »

FASTA

437

47,159

        10         20         30         40         50         60 
MKNIKFSLAW QILFAMVLGI LLGSYLHYHS DSRDWLVVNL LSPAGDIFIH LIKMIVVPIV 

        70         80         90        100        110        120 
ISTLVVGIAG VGDAKQLGRI GAKTIIYFEV ITTVAIILGI TLANVFQPGA GVDMSQLATV 

       130        140        150        160        170        180 
DISKYQSTTE AVQSSSHGIM GTILSLVPTN IVASMAKGEM LPIIFFSVLF GLGLSSLPAT 

       190        200        210        220        230        240 
HREPLVTVFR SISETMFKVT HMVMRYAPVG VFALIAVTVA NFGFSSLWPL AKLVLLVHFA 

       250        260        270        280        290        300 
ILFFALVVLG IVARLCGLSV WILIRILKDE LILAYSTASS ESVLPRIIEK MEAYGAPVSI 

       310        320        330        340        350        360 
TSFVVPTGYS FNLDGSTLYQ SIAAIFIAQL YGIDLSIWQE IILVLTLMVT SKGIAGVPGV 

       370        380        390        400        410        420 
SFVVLLATLG SVGIPLEGLA FIAGVDRILD MARTALNVVG NALAVLVIAK WEHKFDRKKA 

       430 
LAYEREVLGK FDKTADQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a gene encoding a glutamate and aspartate carrier of Escherichia coli K-12." Wallace B., Yang Y.-J., Hong J., Lum D. J. Bacteriol. 172:3214-3220(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Revised nucleotide sequence of the gltP gene, which encodes the proton-glutamate-aspartate transport protein of Escherichia coli K-12." Tolner B., Poolman B., Wallace B., Konings W.N. J. Bacteriol. 174:2391-2393(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION. Strain: K12.
[3]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32488 Genomic DNA. Translation: AAA23832.1. Sequence problems. M84805 Genomic DNA. Translation: AAA24323.1. U00006 Genomic DNA. Translation: AAC43171.1. U00096 Genomic DNA. Translation: AAD13460.1. AP009048 Genomic DNA. Translation: BAE78079.1.
PIR	A42384.
RefSeq	NP_418501.1. NC_000913.2. YP_492220.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P21345.
SMR	P21345. Positions 17-412.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b4077.
Protein family/group databases
TCDB	2.A.23.1.1. dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD13460; AAD13460; b4077. BAE78079; BAE78079; BAE78079.
GeneID	12933685. 948591.
KEGG	ecj:Y75_p3964. eco:b4077.
PATRIC	32123705. VBIEscCol129921_4201.
Organism-specific databases
EchoBASE	EB0400.
EcoGene	EG10405. gltP.
Phylogenomic databases
eggNOG	COG1301.
HOGENOM	HOG000208778.
KO	K11102.
OMA	VMVIAFP.
ProtClustDB	PRK11283.
Enzyme and pathway databases
BioCyc	EcoCyc:GLTP-MONOMER. ECOL316407:JW4038-MONOMER.
Gene expression databases
Genevestigator	P21345.
Family and domain databases
InterPro	IPR001991. Na-dicarboxylate_symporter. IPR018107. Na-dicarboxylate_symporter_CS. [Graphical view]
PANTHER	PTHR11958. PTHR11958. 1 hit.
Pfam	PF00375. SDF. 1 hit. [Graphical view]
PRINTS	PR00173. EDTRNSPORT.
PROSITE	PS00713. NA_DICARBOXYL_SYMP_1. 1 hit. PS00714. NA_DICARBOXYL_SYMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTP_ECOLI

Accession

Primary (citable) accession number: P21345
Secondary accession number(s): Q2M6M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	March 1, 1992
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents