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Protein

Proton glutamate symport protein

Gene

gltP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This carrier protein is part of the Na+-independent, binding-protein-independent glutamate-aspartate transport system.

Enzyme regulationi

Glutamate uptake was inhibited by L-cysteate and DL-3-hydroxyaspartate.

GO - Molecular functioni

GO - Biological processi

  • dicarboxylic acid transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GLTP-MONOMER.
ECOL316407:JW4038-MONOMER.
MetaCyc:GLTP-MONOMER.

Protein family/group databases

TCDBi2.A.23.1.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Proton glutamate symport protein
Alternative name(s):
Glutamate-aspartate carrier protein
Gene namesi
Name:gltP
Ordered Locus Names:b4077, JW4038
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10405. gltP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323CytoplasmicSequence analysisAdd
BLAST
Transmembranei24 – 4623Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini47 – 6519PeriplasmicSequence analysisAdd
BLAST
Transmembranei66 – 8419Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini85 – 9612CytoplasmicSequence analysisAdd
BLAST
Transmembranei97 – 11519Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini116 – 14934PeriplasmicSequence analysisAdd
BLAST
Transmembranei150 – 16819Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini169 – 1713CytoplasmicSequence analysis
Transmembranei172 – 19120Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini192 – 21019PeriplasmicSequence analysisAdd
BLAST
Transmembranei211 – 22919Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini230 – 24213CytoplasmicSequence analysisAdd
BLAST
Transmembranei243 – 26119Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini262 – 28221PeriplasmicSequence analysisAdd
BLAST
Transmembranei283 – 30119Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini302 – 32120CytoplasmicSequence analysisAdd
BLAST
Transmembranei322 – 34019Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini341 – 35111PeriplasmicSequence analysisAdd
BLAST
Transmembranei352 – 37019Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini371 – 3788CytoplasmicSequence analysis
Transmembranei379 – 39820Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini399 – 4057PeriplasmicSequence analysis
Transmembranei406 – 41914Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini420 – 43718CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Proton glutamate symport proteinPRO_0000202088Add
BLAST

Proteomic databases

PaxDbiP21345.

Interactioni

Protein-protein interaction databases

BioGridi4262679. 6 interactions.
STRINGi511145.b4077.

Structurei

3D structure databases

ProteinModelPortaliP21345.
SMRiP21345. Positions 4-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK2. Bacteria.
COG1301. LUCA.
HOGENOMiHOG000208778.
InParanoidiP21345.
KOiK11102.
OMAiICSFVVP.
OrthoDBiEOG69GZN1.
PhylomeDBiP21345.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiIPR033380. GltP/GltT.
IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PTHR11958:SF26. PTHR11958:SF26. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIKFSLAW QILFAMVLGI LLGSYLHYHS DSRDWLVVNL LSPAGDIFIH
60 70 80 90 100
LIKMIVVPIV ISTLVVGIAG VGDAKQLGRI GAKTIIYFEV ITTVAIILGI
110 120 130 140 150
TLANVFQPGA GVDMSQLATV DISKYQSTTE AVQSSSHGIM GTILSLVPTN
160 170 180 190 200
IVASMAKGEM LPIIFFSVLF GLGLSSLPAT HREPLVTVFR SISETMFKVT
210 220 230 240 250
HMVMRYAPVG VFALIAVTVA NFGFSSLWPL AKLVLLVHFA ILFFALVVLG
260 270 280 290 300
IVARLCGLSV WILIRILKDE LILAYSTASS ESVLPRIIEK MEAYGAPVSI
310 320 330 340 350
TSFVVPTGYS FNLDGSTLYQ SIAAIFIAQL YGIDLSIWQE IILVLTLMVT
360 370 380 390 400
SKGIAGVPGV SFVVLLATLG SVGIPLEGLA FIAGVDRILD MARTALNVVG
410 420 430
NALAVLVIAK WEHKFDRKKA LAYEREVLGK FDKTADQ
Length:437
Mass (Da):47,159
Last modified:March 1, 1992 - v2
Checksum:i62E1A5FC16EA54BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32488 Genomic DNA. Translation: AAA23832.1. Sequence problems.
M84805 Genomic DNA. Translation: AAA24323.1.
U00006 Genomic DNA. Translation: AAC43171.1.
U00096 Genomic DNA. Translation: AAD13460.1.
AP009048 Genomic DNA. Translation: BAE78079.1.
PIRiA42384.
RefSeqiNP_418501.1. NC_000913.3.
WP_000789592.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAD13460; AAD13460; b4077.
BAE78079; BAE78079; BAE78079.
GeneIDi948591.
KEGGiecj:JW4038.
eco:b4077.
PATRICi32123705. VBIEscCol129921_4201.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32488 Genomic DNA. Translation: AAA23832.1. Sequence problems.
M84805 Genomic DNA. Translation: AAA24323.1.
U00006 Genomic DNA. Translation: AAC43171.1.
U00096 Genomic DNA. Translation: AAD13460.1.
AP009048 Genomic DNA. Translation: BAE78079.1.
PIRiA42384.
RefSeqiNP_418501.1. NC_000913.3.
WP_000789592.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP21345.
SMRiP21345. Positions 4-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262679. 6 interactions.
STRINGi511145.b4077.

Protein family/group databases

TCDBi2.A.23.1.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Proteomic databases

PaxDbiP21345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD13460; AAD13460; b4077.
BAE78079; BAE78079; BAE78079.
GeneIDi948591.
KEGGiecj:JW4038.
eco:b4077.
PATRICi32123705. VBIEscCol129921_4201.

Organism-specific databases

EchoBASEiEB0400.
EcoGeneiEG10405. gltP.

Phylogenomic databases

eggNOGiENOG4105CK2. Bacteria.
COG1301. LUCA.
HOGENOMiHOG000208778.
InParanoidiP21345.
KOiK11102.
OMAiICSFVVP.
OrthoDBiEOG69GZN1.
PhylomeDBiP21345.

Enzyme and pathway databases

BioCyciEcoCyc:GLTP-MONOMER.
ECOL316407:JW4038-MONOMER.
MetaCyc:GLTP-MONOMER.

Miscellaneous databases

PROiP21345.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiIPR033380. GltP/GltT.
IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PTHR11958:SF26. PTHR11958:SF26. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a gene encoding a glutamate and aspartate carrier of Escherichia coli K-12."
    Wallace B., Yang Y.-J., Hong J., Lum D.
    J. Bacteriol. 172:3214-3220(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Revised nucleotide sequence of the gltP gene, which encodes the proton-glutamate-aspartate transport protein of Escherichia coli K-12."
    Tolner B., Poolman B., Wallace B., Konings W.N.
    J. Bacteriol. 174:2391-2393(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12.
  3. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiGLTP_ECOLI
AccessioniPrimary (citable) accession number: P21345
Secondary accession number(s): Q2M6M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: March 1, 1992
Last modified: May 11, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.