Reviewed, UniProtKB/Swiss-Prot P21343 (PFPB_SOLTU)

Names and origin

Protein names	Recommended name:     Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta       Short name=PFP     EC=2.7.1.90 Alternative name(s):     6-phosphofructokinase, pyrophosphate dependent     Pyrophosphate-dependent 6-phosphofructose-1-kinase     PPi-PFK
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.
Enzyme regulation	Fru-2,6-P is an activator of the plant enzyme.
Subunit structure	Tetramer of two alpha and two beta chains.
Miscellaneous	The active site might be on the beta subunit.
Sequence similarities	Belongs to the pyrophosphate-dependent phosphofructokinase family.
Sequence caution	The sequence AAA63452.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors. Its N-terminal part is probably wrong.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diphosphate-fructose-6-phosphate 1-phosphotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta		PRO_0000112050
Sites
	Active site	214	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21343-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 1AF685A66C023C8E	FASTA	552	60,394
	10 20 30 40 50 60 XQVSGHRRYA AVYSEVQNSR LDHPLPLPSV LGSPFKVVDG PPSSAAGHPE EIAKLFPSLY 70 80 90 100 110 120 GQPHVSLVPD DSGDVAMNQI LKIGVVLSGG QAPGGHNVIS GIFDYLQTHC KGSTMYGFRG 130 140 150 160 170 180 GPAGVMKGKY VVLTPEFIYP YRNQGGFDMI CSGRDKIETP EQFKQAEETA KKLDLDGLVV 190 200 210 220 230 240 IGGDDSNTNA CLLAENFRSK NLKTRVIGCP KTIDGDLKSK EVPTSFGFDT ACKIYAEMIG 250 260 270 280 290 300 NVMIDARSTG KYYHFVRLMG RAASHITLEC ALQTHPNVTL IGEEVFAKKL TLKNVTDYIA 310 320 330 340 350 360 DVVCKRAESG YNYGVILIPE GLIDFIPEVQ QLIAELNEIL AHDVVDEAGV WKKKLTPQCL 370 380 390 400 410 420 ELFELLPLAI QEQLLLERDP HGNVQVAKIE TEKMLIQMVE TELDQRKQKG AYNAQFKGQF 430 440 450 460 470 480 HFFGYEGRCG LPSNFDSTYC YALGYGAGSL LQSGKTGLIS SVGNLAAPVE ELTVGGTALT 490 500 510 520 530 540 ALMDVERRHG KFKPVIKKAM VELEGAPFKK FASKREEWAL NNRYINPGPI QFVGPVANKV 550 NHTLLLELGV DA

References

[1]

"Pyrophosphate-dependent phosphofructokinase. Conservation of protein sequence between the alpha- and beta-subunits and with the ATP-dependent phosphofructokinase." Carlisle S.M., Blakeley S.D., Hemmingsen S.M., Trevanion S.J., Hiyoshi T., Kruger N.J., Dennis D.T. J. Biol. Chem. 265:18366-18371(1990) [PubMed: 2170409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 393-404. Strain: cv. Kennebec.

[2]

"Structure of the genes encoding the alpha- and beta-subunits of castor pyrophosphate-dependent phosphofructokinase." Todd J.F., Blakeley S.D., Dennis D.T. Gene 152:181-186(1995) [PubMed: 7835697] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M55191 mRNA. Translation: AAA63452.1. Sequence problems.
PIR	B36094.
3D structure databases
HSSP	HSSP built from PDB template 1KZH based on UniProtKB P70826.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.90. 296.
Family and domain databases
InterPro	IPR011183. PfpB_PPi_PFK. IPR000023. Phosphofructokinase. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF005677. PPi_PFK_PfpB. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02477. PFKA_PPi. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PFPB_SOLTU

Accession

Primary (citable) accession number: P21343

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)