Reviewed, UniProtKB/Swiss-Prot P21342 (PFPA_SOLTU)

Names and origin

Protein names	Recommended name:     Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha       Short name=PFP     EC=2.7.1.90 Alternative name(s):     6-phosphofructokinase, pyrophosphate dependent     Pyrophosphate-dependent 6-phosphofructose-1-kinase     PPi-PFK
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The alpha subunit may be involved in the regulation of PFP by Fru-2,6-P.
Catalytic activity	Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.
Enzyme regulation	Fru-2,6-P is an activator of the plant enzyme.
Subunit structure	Tetramer of two alpha and two beta chains.
Miscellaneous	The active site might be on the beta subunit.
Sequence similarities	Belongs to the pyrophosphate-dependent phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diphosphate-fructose-6-phosphate 1-phosphotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha		PRO_0000112048

Sequences

	Sequence		Length	Mass (Da)
	P21342-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 2D33EF2ADF3AA0AF	FASTA	616	67,157
	10 20 30 40 50 60 MDADYGIPRE LSDLQKLRSH YHPELPPCLQ GTTVRVELRD ATTAADPAGE HTIKRFFPHT 70 80 90 100 110 120 YGQPLAHFLR ATAKVPDAQI ITEHPAIRVG VLFCGRQSPG GHNVIWGLHD ALKVHNPKNI 130 140 150 160 170 180 LLGFLGGSEG LFAQKTLEIT DDVLATYKNQ GGYDMLGRTK DQIRTTEQVN AAMAACKALK 190 200 210 220 230 240 LDGLVIIGGV TSNTDAAHLA EKFAETKCLT KVVGVPVTLN GDLKNQFVEA NVGFDTICKV 250 260 270 280 290 300 NSQLISNVCT DALSAEKYYY FIRLMGRKAS HVALDCTLQS HPNMVILGEE VAASKLTIFD 310 320 330 340 350 360 ITQQICDAVQ ARAEHDKNHG VILLPEGLIE SIPEVYSLLQ EIHGLLRQGV SADKISSQLS 370 380 390 400 410 420 PWASALFEFL PHFIRKQLLL HPESDDSAQL SQIETEKLIA HLVETEMNKR LKEGTYKGKK 430 440 450 460 470 480 FNAICHFFGY QARGSLPSKF DCDYAYVLGH VCYHILAAGL NGYMATITNL KNPANKWHCG 490 500 510 520 530 540 ASPISAMMTV KRYGRGPGKA SIGVPALHPA TVDLRGKSYE LLSQNATKFL LDDVYRNPGP 550 560 570 580 590 600 LQFDGPGADA KAGSLVVEDA DYIGAIKKLQ EYLDKVRTIV KPGCSQDVLK AALSAMASVT 610 DILSVISSPS SVSTPF

References

[1]

"Pyrophosphate-dependent phosphofructokinase. Conservation of protein sequence between the alpha- and beta-subunits and with the ATP-dependent phosphofructokinase." Carlisle S.M., Blakeley S.D., Hemmingsen S.M., Trevanion S.J., Hiyoshi T., Kruger N.J., Dennis D.T. J. Biol. Chem. 265:18366-18371(1990) [PubMed: 2170409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 277-295. Strain: cv. Kennebec.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M55190 mRNA. Translation: AAA63451.1.
PIR	A36094.
3D structure databases
HSSP	HSSP built from PDB template 1KZH based on UniProtKB P70826.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.90. 296.
Family and domain databases
InterPro	IPR011183. PfpB_PPi_PFK. IPR000023. Phosphofructokinase. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF005677. PPi_PFK_PfpB. 1 hit.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02477. PFKA_PPi. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PFPA_SOLTU

Accession

Primary (citable) accession number: P21342

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)