ID   MSB1_YEAST              Reviewed;        1137 AA.
AC   P21339; D6W2P4;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   27-MAR-2024, entry version 169.
DE   RecName: Full=Morphogenesis-related protein MSB1;
DE   AltName: Full=Multicopy suppressor of bud emergence 1;
GN   Name=MSB1; OrderedLocusNames=YOR188W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1996092; DOI=10.1128/mcb.11.3.1295-1305.1991;
RA   Bender A., Pringle J.R.;
RT   "Use of a screen for synthetic lethal and multicopy suppressee mutants to
RT   identify two new genes involved in morphogenesis in Saccharomyces
RT   cerevisiae.";
RL   Mol. Cell. Biol. 11:1295-1305(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA   Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA   Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA   Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA   Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA   Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA   Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA   Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA   Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA   Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA   Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA   Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA   Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-538 AND SER-816, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-538 AND SER-776, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: May play a role in polarity establishment and bud formation.
CC       The MSB1 gene may be functionally redundant.
CC   -!- INTERACTION:
CC       P21339; P40073: SHO1; NbExp=2; IntAct=EBI-11322, EBI-18140;
CC   -!- MISCELLANEOUS: Present with 172 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; M37767; AAA34797.1; -; Genomic_DNA.
DR   EMBL; Z75096; CAA99397.1; -; Genomic_DNA.
DR   EMBL; BK006948; DAA10960.1; -; Genomic_DNA.
DR   PIR; S13759; S13759.
DR   RefSeq; NP_014831.3; NM_001183607.3.
DR   AlphaFoldDB; P21339; -.
DR   BioGRID; 34583; 83.
DR   DIP; DIP-2687N; -.
DR   IntAct; P21339; 5.
DR   MINT; P21339; -.
DR   STRING; 4932.YOR188W; -.
DR   iPTMnet; P21339; -.
DR   MaxQB; P21339; -.
DR   PaxDb; 4932-YOR188W; -.
DR   PeptideAtlas; P21339; -.
DR   EnsemblFungi; YOR188W_mRNA; YOR188W; YOR188W.
DR   GeneID; 854360; -.
DR   KEGG; sce:YOR188W; -.
DR   AGR; SGD:S000005714; -.
DR   SGD; S000005714; MSB1.
DR   VEuPathDB; FungiDB:YOR188W; -.
DR   eggNOG; ENOG502QUEY; Eukaryota.
DR   HOGENOM; CLU_004952_0_0_1; -.
DR   InParanoid; P21339; -.
DR   OMA; LETHLEM; -.
DR   OrthoDB; 2727882at2759; -.
DR   BioCyc; YEAST:G3O-33698-MONOMER; -.
DR   BioGRID-ORCS; 854360; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P21339; -.
DR   Proteomes; UP000002311; Chromosome XV.
DR   RNAct; P21339; Protein.
DR   GO; GO:0005935; C:cellular bud neck; HDA:SGD.
DR   GO; GO:0005934; C:cellular bud tip; HDA:SGD.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; HDA:SGD.
DR   GO; GO:0030010; P:establishment of cell polarity; IGI:SGD.
DR   CDD; cd04401; RhoGAP_fMSB1; 1.
DR   InterPro; IPR037508; Msb1/Mug8.
DR   InterPro; IPR012965; Msb1/Mug8_dom.
DR   PANTHER; PTHR28093; MORPHOGENESIS-RELATED PROTEIN MSB1; 1.
DR   PANTHER; PTHR28093:SF1; MORPHOGENESIS-RELATED PROTEIN MSB1; 1.
DR   Pfam; PF08101; Msb1-Mug8_dom; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..1137
FT                   /note="Morphogenesis-related protein MSB1"
FT                   /id="PRO_0000096588"
FT   REGION          1..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          483..520
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          577..620
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          736..799
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          814..871
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        25..46
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        577..595
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        596..620
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        747..767
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        768..799
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         538
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         776
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MOD_RES         816
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
SQ   SEQUENCE   1137 AA;  129971 MW;  59CDD6E0F1E43F8A CRC64;
     MNDMAKPLPT PPTAEIRKSR SNSPKKAQKT NLSPNKNQNN EKNVPRSNGR TKNEHNSMDD
     EEFEFFHQFS REKVKGVIHV ITAELKEKGP DVEFLMIPFR PEQTNDKLLT LLNQLFPLGN
     GQPVNEKKQL RIVSKADVWT LFQCLKYIWC RLPNSEIIGW KSYLEFKFRE EDKKFPRKSF
     LEIMPQCLAS PNHASIVYDF FDLIISISSN SRVNKMSARK ISKMCAIWAF SKQIPNSDIQ
     DYDFESAAMK SFAPNNSIQD GLDQWIPASD AMFHLLLAFL RSFVPQDLES AKLPRTLKSL
     LFNNQYPPRK STAYTSETIL TIPLVTLKTD VFSRKPWQLL ERCNDLLDFS DHDAFEARED
     YALLKSLFRK KNTVEGISRK MSQESRRLMK AMSTKHSTFQ PGWAPRECIE NISHLKECIE
     VKRLDIDDYF IWTWLSSLSF EQTSEKKKIF GRSIILEFEF DGFKKWVVFQ ECDITLDYNK
     KGQLKKKTSA QSPTTEKELP PDDFELEDPP LSKSPTLSQT YKKFQAEVPQ QSTVRRDSAP
     DNQGIYHTVI SKNALTKNKH NVNLHSFEHK ISKWNPLNNL RKKSGSNSSS SSFEEKSKDA
     PIREEYHTNK NHKSKKEERV LSQFSTLNPD EYQLPVIETG SSNFKIEIPE LMYEHDDDDS
     DKLKNSQKRA TDSAIEELNG MVEEMMINEP DDVKISITEA ETFESLTKFD QYKPSNITDD
     DLQSSHSSAV HSLKLSTNTN DSCADSSKYT ADRKLAEPRK ISEESKVNDD SSSYYSPNIN
     NLPASRMPSQ PTYSNSDSKK AFTNESRLNV LQGAVSPSQQ VTPKPYKNAP GDCVSPVQQK
     YYQNDRRNEM SPASAPVPPS AYSPARSPQF STNSAGFKQN TINVPVGYND PAHVLANQPH
     MTYRDQHNYP SHQQKQRPFQ NNIVPPELKS RNQRADASPI PQHMVPVKQG VPNLPSNVPL
     YQQMERMNPN HQHPVNTYKV TQPPYHNNTT NAYGNSRAGN AHMLDGKWSN NPPQMVPKGV
     RPNQYPQQHV NRYSPQAQPV VPAEYYNGPP PMRAPPMMSH MVPAQEPIRY TAGANRRSFP
     QGMQQNAYSV PAQPMGAVNS EFYLPEAPQG NKLHGNINKR QERKKLYDNI RSGNFGI
//