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Protein

Ribonuclease I

Gene

rna

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781By similarity
Active sitei152 – 1521By similarity
Active sitei156 – 1561By similarity

GO - Molecular functioni

  • endonuclease activity Source: EcoCyc
  • Enterobacter ribonuclease activity Source: UniProtKB-EC
  • ribonuclease T2 activity Source: InterPro
  • RNA binding Source: InterPro

GO - Biological processi

  • RNA catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BioCyciEcoCyc:EG10856-MONOMER.
ECOL316407:JW0603-MONOMER.
MetaCyc:EG10856-MONOMER.
BRENDAi3.1.27.5. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease I (EC:3.1.27.6)
Short name:
RNase I
Alternative name(s):
Enterobacter ribonuclease
Gene namesi
Name:rna
Synonyms:rnsA
Ordered Locus Names:b0611, JW0603
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10856. rna.

Subcellular locationi

  • Periplasm
  • Cytoplasm

  • Note: RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 268245Ribonuclease IPRO_0000030961Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi103 ↔ 159By similarity

Post-translational modificationi

Contains four disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP21338.
PRIDEiP21338.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4259901. 6 interactions.
IntActiP21338. 1 interaction.
MINTiMINT-1292710.
STRINGi511145.b0611.

Structurei

Secondary structure

1
268
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 313Combined sources
Beta strandi36 – 438Combined sources
Helixi44 – 5310Combined sources
Helixi60 – 634Combined sources
Helixi71 – 744Combined sources
Beta strandi75 – 828Combined sources
Helixi86 – 894Combined sources
Turni90 – 923Combined sources
Helixi95 – 1017Combined sources
Helixi102 – 1043Combined sources
Turni106 – 1094Combined sources
Helixi116 – 1216Combined sources
Helixi129 – 13810Combined sources
Turni144 – 1463Combined sources
Helixi148 – 1569Combined sources
Helixi158 – 1603Combined sources
Helixi164 – 18017Combined sources
Helixi182 – 1898Combined sources
Turni190 – 1923Combined sources
Beta strandi193 – 1964Combined sources
Helixi197 – 20812Combined sources
Helixi210 – 2156Combined sources
Beta strandi216 – 2216Combined sources
Turni222 – 2254Combined sources
Beta strandi226 – 23510Combined sources
Helixi236 – 2383Combined sources
Beta strandi255 – 2573Combined sources
Beta strandi259 – 2624Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EA1X-ray1.80A24-268[»]
2PQXX-ray1.42A24-268[»]
2PQYX-ray2.30A24-268[»]
2Z70X-ray1.70A24-268[»]
ProteinModelPortaliP21338.
SMRiP21338. Positions 24-268.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21338.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase T2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105KTS. Bacteria.
COG3719. LUCA.
HOGENOMiHOG000117890.
InParanoidiP21338.
KOiK01169.
OMAiTRPIPNM.
OrthoDBiEOG6MWN93.

Family and domain databases

Gene3Di3.90.730.10. 1 hit.
InterProiIPR001568. RNase_T2-like.
IPR018188. RNase_T2_His_AS_1.
IPR033130. RNase_T2_His_AS_2.
[Graphical view]
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAFWRNAAL LAVSLLPFSS ANALALQAKQ YGDFDRYVLA LSWQTGFCQS
60 70 80 90 100
QHDRNRNERD ECRLQTETTN KADFLTVHGL WPGLPKSVAA RGVDERRWMR
110 120 130 140 150
FGCATRPIPN LPEARASRMC SSPETGLSLE TAAKLSEVMP GAGGRSCLER
160 170 180 190 200
YEYAKHGACF GFDPDAYFGT MVRLNQEIKE SEAGKFLADN YGKTVSRRDF
210 220 230 240 250
DAAFAKSWGK ENVKAVKLTC QGNPAYLTEI QISIKADAIN APLSANSFLP
260
QPHPGNCGKT FVIDKAGY
Length:268
Mass (Da):29,618
Last modified:May 1, 1991 - v1
Checksum:i56BC290438EE3DD0
GO

Sequence cautioni

The sequence AAB40811.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55687 Genomic DNA. Translation: AAA24548.1.
U82598 Genomic DNA. Translation: AAB40811.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73712.1.
AP009048 Genomic DNA. Translation: BAA35240.1.
PIRiJQ0777.
RefSeqiNP_415144.1. NC_000913.3.
WP_000643397.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73712; AAC73712; b0611.
BAA35240; BAA35240; BAA35240.
GeneIDi949065.
KEGGiecj:JW0603.
eco:b0611.
PATRICi32116402. VBIEscCol129921_0641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55687 Genomic DNA. Translation: AAA24548.1.
U82598 Genomic DNA. Translation: AAB40811.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73712.1.
AP009048 Genomic DNA. Translation: BAA35240.1.
PIRiJQ0777.
RefSeqiNP_415144.1. NC_000913.3.
WP_000643397.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EA1X-ray1.80A24-268[»]
2PQXX-ray1.42A24-268[»]
2PQYX-ray2.30A24-268[»]
2Z70X-ray1.70A24-268[»]
ProteinModelPortaliP21338.
SMRiP21338. Positions 24-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259901. 6 interactions.
IntActiP21338. 1 interaction.
MINTiMINT-1292710.
STRINGi511145.b0611.

Proteomic databases

PaxDbiP21338.
PRIDEiP21338.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73712; AAC73712; b0611.
BAA35240; BAA35240; BAA35240.
GeneIDi949065.
KEGGiecj:JW0603.
eco:b0611.
PATRICi32116402. VBIEscCol129921_0641.

Organism-specific databases

EchoBASEiEB0849.
EcoGeneiEG10856. rna.

Phylogenomic databases

eggNOGiENOG4105KTS. Bacteria.
COG3719. LUCA.
HOGENOMiHOG000117890.
InParanoidiP21338.
KOiK01169.
OMAiTRPIPNM.
OrthoDBiEOG6MWN93.

Enzyme and pathway databases

BioCyciEcoCyc:EG10856-MONOMER.
ECOL316407:JW0603-MONOMER.
MetaCyc:EG10856-MONOMER.
BRENDAi3.1.27.5. 2026.

Miscellaneous databases

EvolutionaryTraceiP21338.
PROiP21338.

Family and domain databases

Gene3Di3.90.730.10. 1 hit.
InterProiIPR001568. RNase_T2-like.
IPR018188. RNase_T2_His_AS_1.
IPR033130. RNase_T2_His_AS_2.
[Graphical view]
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing the gene encoding Escherichia coli ribonuclease I: exact physical mapping using the genome library."
    Meador J. III, Kennell D.
    Gene 95:1-7(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-63.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Protein family classification based on searching a database of blocks."
    Henikoff S., Henikoff J.G.
    Genomics 19:97-107(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO FUNGAL RIBONUCLEASES.

Entry informationi

Entry nameiRNI_ECOLI
AccessioniPrimary (citable) accession number: P21338
Secondary accession number(s): P77101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: July 6, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.