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P21335

- TADA_BACSU

UniProt

P21335 - TADA_BACSU

Protein

tRNA-specific adenosine deaminase

Gene

tadA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).UniRule annotation

    Catalytic activityi

    Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Zinc; via pros nitrogen; catalyticUniRule annotation
    Active sitei55 – 551Proton donorUniRule annotation
    Metal bindingi83 – 831Zinc; catalyticUniRule annotation
    Metal bindingi86 – 861Zinc; catalyticUniRule annotation

    GO - Molecular functioni

    1. tRNA-specific adenosine-34 deaminase activity Source: UniProtKB-HAMAP
    2. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA wobble adenosine to inosine editing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBSUB:BSU00180-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-specific adenosine deaminaseUniRule annotation (EC:3.5.4.33UniRule annotation)
    Gene namesi
    Name:tadAUniRule annotation
    Synonyms:yaaJ
    Ordered Locus Names:BSU00180
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU00180. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 161161tRNA-specific adenosine deaminasePRO_0000171705Add
    BLAST

    Proteomic databases

    PaxDbiP21335.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224308.BSU00180.

    Structurei

    3D structure databases

    ProteinModelPortaliP21335.
    SMRiP21335. Positions 1-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 101101CMP/dCMP deaminase zinc-bindingUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cytidine and deoxycytidylate deaminase family.UniRule annotation
    Contains 1 CMP/dCMP deaminase zinc-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0590.
    HOGENOMiHOG000085050.
    KOiK11991.
    OMAiIMCAGAM.
    OrthoDBiEOG64FKGZ.
    PhylomeDBiP21335.

    Family and domain databases

    HAMAPiMF_00972. tRNA_aden_deaminase.
    InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR002125. CMP_dCMP_Zn-bd.
    IPR016193. Cytidine_deaminase-like.
    IPR028883. tRNA_aden_deaminase.
    [Graphical view]
    PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53927. SSF53927. 1 hit.
    PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21335-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQDELYMKE AIKEAKKAEE KGEVPIGAVL VINGEIIARA HNLRETEQRS    50
    IAHAEMLVID EACKALGTWR LEGATLYVTL EPCPMCAGAV VLSRVEKVVF 100
    GAFDPKGGCS GTLMNLLQEE RFNHQAEVVS GVLEEECGGM LSAFFRELRK 150
    KKKAARKNLS E 161
    Length:161
    Mass (Da):17,752
    Last modified:May 1, 1991 - v1
    Checksum:i3256F31DF6610FBB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52144 Genomic DNA. Translation: CAA36389.1.
    D26185 Genomic DNA. Translation: BAA05254.1.
    AL009126 Genomic DNA. Translation: CAB11794.1.
    PIRiS11690.
    RefSeqiNP_387899.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB11794; CAB11794; BSU00180.
    GeneIDi937989.
    KEGGibsu:BSU00180.
    PATRICi18971497. VBIBacSub10457_0019.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52144 Genomic DNA. Translation: CAA36389.1 .
    D26185 Genomic DNA. Translation: BAA05254.1 .
    AL009126 Genomic DNA. Translation: CAB11794.1 .
    PIRi S11690.
    RefSeqi NP_387899.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P21335.
    SMRi P21335. Positions 1-149.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU00180.

    Proteomic databases

    PaxDbi P21335.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB11794 ; CAB11794 ; BSU00180 .
    GeneIDi 937989.
    KEGGi bsu:BSU00180.
    PATRICi 18971497. VBIBacSub10457_0019.

    Organism-specific databases

    GenoListi BSU00180. [Micado ]

    Phylogenomic databases

    eggNOGi COG0590.
    HOGENOMi HOG000085050.
    KOi K11991.
    OMAi IMCAGAM.
    OrthoDBi EOG64FKGZ.
    PhylomeDBi P21335.

    Enzyme and pathway databases

    BioCyci BSUB:BSU00180-MONOMER.

    Family and domain databases

    HAMAPi MF_00972. tRNA_aden_deaminase.
    InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR002125. CMP_dCMP_Zn-bd.
    IPR016193. Cytidine_deaminase-like.
    IPR028883. tRNA_aden_deaminase.
    [Graphical view ]
    Pfami PF00383. dCMP_cyt_deam_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53927. SSF53927. 1 hit.
    PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a 17 kDa protein gene upstream from the small cytoplasmic RNA gene of Bacillus subtilis."
      Struck J.C.R., Kretschmer-Kazemi F.R., Schroeder W., Hucho F., Toschka H.Y., Erdmann V.A.
      Biochim. Biophys. Acta 1050:80-83(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-16.
      Strain: 168.
    2. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
      Ogasawara N., Nakai S., Yoshikawa H.
      DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiTADA_BACSU
    AccessioniPrimary (citable) accession number: P21335
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3