P21335 (TADA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-specific adenosine deaminase
EC=3.5.4.-

Gene names

Name:	tadA
Synonyms:	yaaJ
Ordered Locus Names:	BSU00180

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Deaminates adenosine-34 to inosine in tRNA-Arg2 By similarity.
Catalytic activity	Adenosine + H2O = inosine + NH3.
Cofactor	Binds 1 zinc ion per subunit.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	tRNA-specific adenosine deaminase		PRO_0000171705
Sites
	Active site	55	1	Proton donor By similarity
	Metal binding	53	1	Zinc; catalytic By similarity
	Metal binding	83	1	Zinc; catalytic By similarity
	Metal binding	86	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21335 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 3256F31DF6610FBB	FASTA	161	17,752
	10 20 30 40 50 60 MTQDELYMKE AIKEAKKAEE KGEVPIGAVL VINGEIIARA HNLRETEQRS IAHAEMLVID 70 80 90 100 110 120 EACKALGTWR LEGATLYVTL EPCPMCAGAV VLSRVEKVVF GAFDPKGGCS GTLMNLLQEE 130 140 150 160 RFNHQAEVVS GVLEEECGGM LSAFFRELRK KKKAARKNLS E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a 17 kDa protein gene upstream from the small cytoplasmic RNA gene of Bacillus subtilis." Struck J.C.R., Kretschmer-Kazemi F.R., Schroeder W., Hucho F., Toschka H.Y., Erdmann V.A. Biochim. Biophys. Acta 1050:80-83(1990) [PubMed: 1698458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-16. Strain: 168.
[2]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52144 Genomic DNA. Translation: CAA36389.1. D26185 Genomic DNA. Translation: BAA05254.1. AL009126 Genomic DNA. Translation: CAB11794.1.
PIR	S11690.
RefSeq	NP_387899.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P21335.
SMR	P21335. Positions 1-149.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000000664; EBBACP00000000664; EBBACG00000000662.
GeneID	937989.
GenomeReviews	Gene locus BSU00180 in contig AL009126_GR.
KEGG	bsu:BSU00180.
NMPDR	fig|224308.1.peg.18.
PATRIC	18971497. VBIBacSub10457_0019.
Organism-specific databases
GenoList	BSU00180. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002012.
HOGENOM	HBG741046.
OMA	DACSAQL.
PhylomeDB	P21335.
ProtClustDB	CLSK886547.
Enzyme and pathway databases
BioCyc	BSUB:BSU00180-MONOMER.
Family and domain databases
InterPro	IPR016192. APOBEC/CMP_deaminase_Zn-bd. IPR002125. CMP_dCMP_Zn-bd. IPR016193. Cytidine_deaminase-like. [Graphical view]
Pfam	PF00383. dCMP_cyt_deam_1. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit.
PROSITE	PS00903. CYT_DCMP_DEAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TADA_BACSU

Accession

Primary (citable) accession number: P21335

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program