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Protein

tRNA-specific adenosine deaminase

Gene

tadA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).UniRule annotation

Catalytic activityi

Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Zinc; via pros nitrogen; catalyticUniRule annotation
Active sitei55 – 551Proton donorUniRule annotation
Metal bindingi83 – 831Zinc; catalyticUniRule annotation
Metal bindingi86 – 861Zinc; catalyticUniRule annotation

GO - Molecular functioni

  1. tRNA-specific adenosine-34 deaminase activity Source: UniProtKB-HAMAP
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA wobble adenosine to inosine editing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU00180-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminaseUniRule annotation (EC:3.5.4.33UniRule annotation)
Gene namesi
Name:tadAUniRule annotation
Synonyms:yaaJ
Ordered Locus Names:BSU00180
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU00180. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161tRNA-specific adenosine deaminasePRO_0000171705Add
BLAST

Proteomic databases

PaxDbiP21335.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224308.BSU00180.

Structurei

3D structure databases

ProteinModelPortaliP21335.
SMRiP21335. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 101101CMP/dCMP deaminase zinc-bindingUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the cytidine and deoxycytidylate deaminase family.UniRule annotation
Contains 1 CMP/dCMP deaminase zinc-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0590.
HOGENOMiHOG000085050.
InParanoidiP21335.
KOiK11991.
OMAiHRVEISE.
OrthoDBiEOG64FKGZ.
PhylomeDBiP21335.

Family and domain databases

HAMAPiMF_00972. tRNA_aden_deaminase.
InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR028883. tRNA_aden_deaminase.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21335-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQDELYMKE AIKEAKKAEE KGEVPIGAVL VINGEIIARA HNLRETEQRS
60 70 80 90 100
IAHAEMLVID EACKALGTWR LEGATLYVTL EPCPMCAGAV VLSRVEKVVF
110 120 130 140 150
GAFDPKGGCS GTLMNLLQEE RFNHQAEVVS GVLEEECGGM LSAFFRELRK
160
KKKAARKNLS E
Length:161
Mass (Da):17,752
Last modified:May 1, 1991 - v1
Checksum:i3256F31DF6610FBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52144 Genomic DNA. Translation: CAA36389.1.
D26185 Genomic DNA. Translation: BAA05254.1.
AL009126 Genomic DNA. Translation: CAB11794.1.
PIRiS11690.
RefSeqiNP_387899.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB11794; CAB11794; BSU00180.
GeneIDi937989.
KEGGibsu:BSU00180.
PATRICi18971497. VBIBacSub10457_0019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52144 Genomic DNA. Translation: CAA36389.1.
D26185 Genomic DNA. Translation: BAA05254.1.
AL009126 Genomic DNA. Translation: CAB11794.1.
PIRiS11690.
RefSeqiNP_387899.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP21335.
SMRiP21335. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU00180.

Proteomic databases

PaxDbiP21335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11794; CAB11794; BSU00180.
GeneIDi937989.
KEGGibsu:BSU00180.
PATRICi18971497. VBIBacSub10457_0019.

Organism-specific databases

GenoListiBSU00180. [Micado]

Phylogenomic databases

eggNOGiCOG0590.
HOGENOMiHOG000085050.
InParanoidiP21335.
KOiK11991.
OMAiHRVEISE.
OrthoDBiEOG64FKGZ.
PhylomeDBiP21335.

Enzyme and pathway databases

BioCyciBSUB:BSU00180-MONOMER.

Family and domain databases

HAMAPiMF_00972. tRNA_aden_deaminase.
InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR028883. tRNA_aden_deaminase.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a 17 kDa protein gene upstream from the small cytoplasmic RNA gene of Bacillus subtilis."
    Struck J.C.R., Kretschmer-Kazemi F.R., Schroeder W., Hucho F., Toschka H.Y., Erdmann V.A.
    Biochim. Biophys. Acta 1050:80-83(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-16.
    Strain: 168.
  2. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiTADA_BACSU
AccessioniPrimary (citable) accession number: P21335
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.