Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P21327

- INPP_BOVIN

UniProt

P21327 - INPP_BOVIN

Protein

Inositol polyphosphate 1-phosphatase

Gene

INPP1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    1D-myo-inositol 1,4-bisphosphate + H2O = 1D-myo-inositol 4-phosphate + phosphate.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Inhibited by Li+.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi79 – 791Magnesium 1
    Binding sitei119 – 1191SubstrateBy similarity
    Metal bindingi153 – 1531Magnesium 1By similarity
    Metal bindingi153 – 1531Magnesium 2
    Metal bindingi155 – 1551Magnesium 1; via carbonyl oxygen
    Metal bindingi156 – 1561Magnesium 2
    Metal bindingi317 – 3171Magnesium 2
    Binding sitei317 – 3171SubstrateBy similarity

    GO - Molecular functioni

    1. inositol-1,4-bisphosphate 1-phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. phosphatidylinositol phosphorylation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Lithium, Magnesium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_212018. Synthesis of IP2, IP, and Ins in the cytosol.
    SABIO-RKP21327.
    UniPathwayiUPA00944.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol polyphosphate 1-phosphatase (EC:3.1.3.57)
    Short name:
    IPP
    Short name:
    IPPase
    Gene namesi
    Name:INPP1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Inositol polyphosphate 1-phosphatasePRO_0000142509Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei318 – 3181PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP21327.
    PRIDEiP21327.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000009977.

    Structurei

    Secondary structure

    1
    400
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 2421
    Helixi26 – 294
    Turni40 – 434
    Helixi48 – 6821
    Helixi72 – 754
    Beta strandi76 – 805
    Beta strandi82 – 854
    Beta strandi91 – 933
    Helixi103 – 1108
    Turni111 – 1133
    Helixi117 – 1259
    Beta strandi134 – 1385
    Helixi145 – 1473
    Beta strandi148 – 1569
    Helixi158 – 1636
    Beta strandi176 – 1783
    Helixi179 – 1813
    Beta strandi183 – 1908
    Turni191 – 1933
    Beta strandi196 – 20813
    Turni210 – 2123
    Beta strandi215 – 23016
    Turni240 – 2423
    Beta strandi258 – 2614
    Beta strandi264 – 2674
    Turni278 – 2814
    Beta strandi284 – 2874
    Helixi291 – 2999
    Beta strandi304 – 3085
    Helixi315 – 32612
    Turni327 – 3293
    Beta strandi331 – 3344
    Helixi335 – 3406
    Turni359 – 3646
    Beta strandi373 – 3775
    Helixi379 – 3857
    Turni390 – 3923

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1INPX-ray2.30A1-400[»]
    ProteinModelPortaliP21327.
    SMRiP21327. Positions 1-400.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21327.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni155 – 1584Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the inositol monophosphatase family.Curated

    Phylogenomic databases

    eggNOGiNOG306469.
    GeneTreeiENSGT00390000014061.
    HOGENOMiHOG000007839.
    HOVERGENiHBG006163.
    InParanoidiP21327.
    KOiK01107.
    OMAiAGYKSLC.
    OrthoDBiEOG7G4QG6.
    TreeFamiTF314300.

    Family and domain databases

    InterProiIPR020583. Inositol_monoP_metal-BS.
    IPR000760. Inositol_monophosphatase.
    IPR020550. Inositol_monophosphatase_CS.
    [Graphical view]
    PANTHERiPTHR20854. PTHR20854. 1 hit.
    PfamiPF00459. Inositol_P. 1 hit.
    [Graphical view]
    PROSITEiPS00629. IMP_1. 1 hit.
    PS00630. IMP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21327-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDILQELLR VSEKAANIAR ACRQQETLFQ LLIEEKKEGE KNKKFAVDFK    50
    TLADVLVQEV IKENMENKFP GLGKKIFGEE SNEFTNDLGE KIIMRLGPTE 100
    EETVALLSKV LNGNKLASEA LAKVVHQDVF FSDPALDSVE INIPQDILGI 150
    WVDPIDSTYQ YIKGSADITP NQGIFPSGLQ CVTVLIGVYD IQTGVPLMGV 200
    INQPFVSQDL HTRRWKGQCY WGLSYLGTNI HSLLPPVSTR SNSEAQSQGT 250
    QNPSSEGSCR FSVVISTSEK ETIKGALSHV CGERIFRAAG AGYKSLCVIL 300
    GLADIYIFSE DTTFKWDSCA AHAILRAMGG GMVDLKECLE RNPDTGLDLP 350
    QLVYHVGNEG AAGVDQWANK GGLIAYRSEK QLETFLSRLL QHLAPVATHT 400
    Length:400
    Mass (Da):43,965
    Last modified:October 17, 2006 - v2
    Checksum:i27917063A224D6A8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti84 – 841F → L in AAA30588. (PubMed:2175905)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55916 mRNA. Translation: AAA30588.1.
    BC114863 mRNA. Translation: AAI14864.1.
    PIRiA39254.
    RefSeqiNP_776789.1. NM_174364.2.
    XP_005202248.1. XM_005202191.1.
    XP_005202249.1. XM_005202192.1.
    XP_005202250.1. XM_005202193.1.
    XP_005202251.1. XM_005202194.1.
    UniGeneiBt.5174.

    Genome annotation databases

    EnsembliENSBTAT00000009977; ENSBTAP00000009977; ENSBTAG00000007584.
    GeneIDi281869.
    KEGGibta:281869.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55916 mRNA. Translation: AAA30588.1 .
    BC114863 mRNA. Translation: AAI14864.1 .
    PIRi A39254.
    RefSeqi NP_776789.1. NM_174364.2.
    XP_005202248.1. XM_005202191.1.
    XP_005202249.1. XM_005202192.1.
    XP_005202250.1. XM_005202193.1.
    XP_005202251.1. XM_005202194.1.
    UniGenei Bt.5174.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1INP X-ray 2.30 A 1-400 [» ]
    ProteinModelPortali P21327.
    SMRi P21327. Positions 1-400.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000009977.

    Proteomic databases

    PaxDbi P21327.
    PRIDEi P21327.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000009977 ; ENSBTAP00000009977 ; ENSBTAG00000007584 .
    GeneIDi 281869.
    KEGGi bta:281869.

    Organism-specific databases

    CTDi 3628.

    Phylogenomic databases

    eggNOGi NOG306469.
    GeneTreei ENSGT00390000014061.
    HOGENOMi HOG000007839.
    HOVERGENi HBG006163.
    InParanoidi P21327.
    KOi K01107.
    OMAi AGYKSLC.
    OrthoDBi EOG7G4QG6.
    TreeFami TF314300.

    Enzyme and pathway databases

    UniPathwayi UPA00944 .
    Reactomei REACT_212018. Synthesis of IP2, IP, and Ins in the cytosol.
    SABIO-RK P21327.

    Miscellaneous databases

    EvolutionaryTracei P21327.
    NextBioi 20805771.

    Family and domain databases

    InterProi IPR020583. Inositol_monoP_metal-BS.
    IPR000760. Inositol_monophosphatase.
    IPR020550. Inositol_monophosphatase_CS.
    [Graphical view ]
    PANTHERi PTHR20854. PTHR20854. 1 hit.
    Pfami PF00459. Inositol_P. 1 hit.
    [Graphical view ]
    PROSITEi PS00629. IMP_1. 1 hit.
    PS00630. IMP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and heterologous expression of a cDNA encoding bovine inositol polyphosphate 1-phosphatase."
      York J.D., Majerus P.W.
      Proc. Natl. Acad. Sci. U.S.A. 87:9548-9552(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Brain.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Thymus.
    3. "Crystal structure of inositol polyphosphate 1-phosphatase at 2.3-A resolution."
      York J.D., Ponder J.W., Chen Z.-W., Mathews F.S., Majerus P.W.
      Biochemistry 33:13164-13171(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS.

    Entry informationi

    Entry nameiINPP_BOVIN
    AccessioniPrimary (citable) accession number: P21327
    Secondary accession number(s): Q1RMJ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Acts on inositol 1,4-bisphosphate and inositol 1,3,4-trisphosphate (forming inositol 3,4-bisphosphate) with a 4-fold higher affinity for the bisphosphate.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3