Reviewed, UniProtKB/Swiss-Prot P21326 (RIPP_MIRJA)

Names and origin

Protein names	Recommended name:     Antiviral protein MAP     EC=3.2.2.22 Alternative name(s):     MAP-S     Ribosome-inactivating protein     rRNA N-glycosidase
Organism	Mirabilis jalapa (Garden four-o'clock)
Taxonomic identifier	3538 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Nyctaginaceae › Mirabilis

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits viral infection of plants, and protein synthesis in vitro.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.
Mass spectrometry	Molecular mass is 27789±2 Da from positions 29 - 278. Determined by ESI. Ref.4

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Ref.4 Ref.3
	Chain	29 – 278	250	Antiviral protein MAP		PRO_0000030778
Sites
	Active site	196	1	By similarity
Amino acid modifications
	Disulfide bond	64 ↔ 248		Ref.4
Experimental info
	Sequence conflict	35	1	I → L in BAA01079. Ref.1
	Sequence conflict	58	1	A → V in BAA01079. Ref.1
	Sequence conflict	62	1	E → L AA sequence Ref.4
	Sequence conflict	180	1	V → C in BAA01079. Ref.1
	Sequence conflict	189	1	L → I AA sequence Ref.4
	Sequence conflict	213	1	E → D AA sequence Ref.4
	Sequence conflict	218	1	D → G in BAA01079. Ref.1
	Sequence conflict	219	1	E → D AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P21326-1 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: BDB9E08E65D2F92D	FASTA	278	31,132
	10 20 30 40 50 60 MLTTTKVFFL LLTTWITWYA IVNPQSRAAP TLETIASLDL NNPTTYLSFI TNIRTKVADK 70 80 90 100 110 120 TEQCTIQKIS KTFTQRYSYI DLIVSSTQKI TLAIDMADLY VLGYSDIANN KGRAFFFKDV 130 140 150 160 170 180 TEAVANNFFP GATGTNRIKL TFTGSYGDLE KNGGLRKDNP LGIFRLENSI VNIYGKAGDV 190 200 210 220 230 240 KKQAKFFLLA IQMVSEAARF KYISDKIPSE KYEEVTVDEY MTALENNWAK LSTAVYNSKP 250 260 270 STTTATKCQL ATSPVTISPW IFKTVEEIKL VMGLLKSS

References

[1]	"DNA sequence of Mirabilis antiviral protein (MAP), a ribosome-inactivating protein with an antiviral property, from mirabilis jalapa L. and its expression in Escherichia coli." Kataoka J., Habuka N., Furuno M., Miyano M., Takanami Y., Koiwai A. J. Biol. Chem. 266:8426-8430(1991) [PubMed: 2022657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A genomic gene for MAP, a ribosome-inactivating protein from Mirabilis jalapa, contains an intron." Kataoka J., Miyano M., Habuka N., Masuta C., Koiwai A. Nucleic Acids Res. 21:1035-1035(1993) [PubMed: 8451171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Amino acid sequence of Mirabilis antiviral protein, total synthesis of its gene and expression in Escherichia coli." Habuka N., Murakami Y., Noma M., Kudo T., Horikoshi K. J. Biol. Chem. 264:6629-6637(1989) [PubMed: 2708328] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-278.
[4]	"Reliable sequence determination of ribosome-inactivating proteins by combining electrospray mass spectrometry and Edman degradation." Di Maro A., Ferranti P., Mastronicola M., Polito L., Bolognesi A., Stirpe F., Malorni A., Parente A. J. Mass Spectrom. 36:38-46(2001) [PubMed: 11180645] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-278, MASS SPECTROMETRY, DISULFIDE BOND. Tissue: Seed.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10227 mRNA. Translation: BAA01079.1. D10569 Genomic DNA. Translation: BAA01425.1.
PIR	A39817. S35539.
3D structure databases
SMR	P21326. Positions 36-276.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273561.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIPP_MIRJA

Accession

Primary (citable) accession number: P21326

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	December 1, 1992
Last modified:	January 19, 2010
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)