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P21311

- DMA7_ECOLX

UniProt

P21311 - DMA7_ECOLX

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Protein
Retron EC67 DNA adenine methylase
Gene
N/A
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71S-adenosyl-L-methionine By similarity
Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei51 – 511S-adenosyl-L-methionine By similarity
Binding sitei179 – 1791S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi2846. M.EcoEc67Dam.

Names & Taxonomyi

Protein namesi
Recommended name:
Retron EC67 DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
M.Eco67Dam
ORF1-EC67 DAM
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Retron EC67 DNA adenine methylase
PRO_0000087995Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP21311.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0338.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21311-1 [UniParc]FASTAAdd to Basket

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MSTILKWAGN KTAIMSELKK HLPAGPRLVE PFAGSCAVMM ATDYPSYLVA    50
DINPDLINLY KKIAADCEAF ISRARVLFEI ANREVAYYNI RQEFNYSTEI 100
TDFMKAVYFL YLNRHGYRGL CRYNKSGHFN IPYGNYKNPY FPEKEIRKFA 150
EKAQRATFIC ASFDETLAML QVGDVVYCDP PYDGTFSGYH TDGFTEDDQY 200
HLASVLEYRS SEGHPVIVSN SDTSLIRSLY RNFTHHYIKA KRSIGVSAGE 250
SKSATEIIAV SGARCWVGFD PSRGVDSSAV YEVRV 285
Length:285
Mass (Da):32,255
Last modified:May 1, 1991 - v1
Checksum:i2F7F5FF2804F4841
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55249 Genomic DNA. Translation: AAA23396.1.
PIRiJQ0851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55249 Genomic DNA. Translation: AAA23396.1 .
PIRi JQ0851.

3D structure databases

ProteinModelPortali P21311.
ModBasei Search...

Protein family/group databases

REBASEi 2846. M.EcoEc67Dam.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0338.

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Retron for the 67-base multicopy single-stranded DNA from Escherichia coli: a potential transposable element encoding both reverse transcriptase and Dam methylase functions."
    Hsu M.-Y., Inouye M., Inouye S.
    Proc. Natl. Acad. Sci. U.S.A. 87:9454-9458(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: O1:NM / CL-1.

Entry informationi

Entry nameiDMA7_ECOLX
AccessioniPrimary (citable) accession number: P21311
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Transposable element

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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