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Protein

Retron EC67 DNA adenine methylase

Gene
N/A
Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron.

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7S-adenosyl-L-methionineBy similarity1
Binding sitei11S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei51S-adenosyl-L-methionineBy similarity1
Binding sitei179S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi2846. M.EcoEc67Dam.

Names & Taxonomyi

Protein namesi
Recommended name:
Retron EC67 DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
M.Eco67Dam
ORF1-EC67 DAM
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879951 – 285Retron EC67 DNA adenine methylaseAdd BLAST285

Interactioni

Protein-protein interaction databases

STRINGi199310.c0941.

Structurei

3D structure databases

ProteinModelPortaliP21311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105DFE. Bacteria.
COG0338. LUCA.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTILKWAGN KTAIMSELKK HLPAGPRLVE PFAGSCAVMM ATDYPSYLVA
60 70 80 90 100
DINPDLINLY KKIAADCEAF ISRARVLFEI ANREVAYYNI RQEFNYSTEI
110 120 130 140 150
TDFMKAVYFL YLNRHGYRGL CRYNKSGHFN IPYGNYKNPY FPEKEIRKFA
160 170 180 190 200
EKAQRATFIC ASFDETLAML QVGDVVYCDP PYDGTFSGYH TDGFTEDDQY
210 220 230 240 250
HLASVLEYRS SEGHPVIVSN SDTSLIRSLY RNFTHHYIKA KRSIGVSAGE
260 270 280
SKSATEIIAV SGARCWVGFD PSRGVDSSAV YEVRV
Length:285
Mass (Da):32,255
Last modified:May 1, 1991 - v1
Checksum:i2F7F5FF2804F4841
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55249 Genomic DNA. Translation: AAA23396.1.
PIRiJQ0851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55249 Genomic DNA. Translation: AAA23396.1.
PIRiJQ0851.

3D structure databases

ProteinModelPortaliP21311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0941.

Protein family/group databases

REBASEi2846. M.EcoEc67Dam.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105DFE. Bacteria.
COG0338. LUCA.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDMA7_ECOLX
AccessioniPrimary (citable) accession number: P21311
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Transposable element

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.