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P21311

- DMA7_ECOLX

UniProt

P21311 - DMA7_ECOLX

Protein

Retron EC67 DNA adenine methylase

Gene
N/A
Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    • Comment

    Functioni

    Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei7 – 71S-adenosyl-L-methionineBy similarity
    Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei51 – 511S-adenosyl-L-methionineBy similarity
    Binding sitei179 – 1791S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi2846. M.EcoEc67Dam.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Retron EC67 DNA adenine methylase (EC:2.1.1.72)
    Alternative name(s):
    M.Eco67Dam
    ORF1-EC67 DAM
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Retron EC67 DNA adenine methylasePRO_0000087995Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP21311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0338.

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21311-1 [UniParc]FASTAAdd to Basket

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    MSTILKWAGN KTAIMSELKK HLPAGPRLVE PFAGSCAVMM ATDYPSYLVA    50
    DINPDLINLY KKIAADCEAF ISRARVLFEI ANREVAYYNI RQEFNYSTEI 100
    TDFMKAVYFL YLNRHGYRGL CRYNKSGHFN IPYGNYKNPY FPEKEIRKFA 150
    EKAQRATFIC ASFDETLAML QVGDVVYCDP PYDGTFSGYH TDGFTEDDQY 200
    HLASVLEYRS SEGHPVIVSN SDTSLIRSLY RNFTHHYIKA KRSIGVSAGE 250
    SKSATEIIAV SGARCWVGFD PSRGVDSSAV YEVRV 285
    Length:285
    Mass (Da):32,255
    Last modified:May 1, 1991 - v1
    Checksum:i2F7F5FF2804F4841
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55249 Genomic DNA. Translation: AAA23396.1.
    PIRiJQ0851.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55249 Genomic DNA. Translation: AAA23396.1 .
    PIRi JQ0851.

    3D structure databases

    ProteinModelPortali P21311.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 2846. M.EcoEc67Dam.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0338.

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Retron for the 67-base multicopy single-stranded DNA from Escherichia coli: a potential transposable element encoding both reverse transcriptase and Dam methylase functions."
      Hsu M.-Y., Inouye M., Inouye S.
      Proc. Natl. Acad. Sci. U.S.A. 87:9454-9458(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: O1:NM / CL-1.

    Entry informationi

    Entry nameiDMA7_ECOLX
    AccessioniPrimary (citable) accession number: P21311
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Transposable element

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3