Reviewed, UniProtKB/Swiss-Prot P21311 (DMA7_ECOLX)

Names and origin

Protein names	Recommended name:     Retron EC67 DNA adenine methylase     EC=2.1.1.72 Alternative name(s):     M.Eco67Dam     ORF1-EC67 DAM
Organism	Escherichia coli
Taxonomic identifier	562 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Transposable element
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Retron EC67 DNA adenine methylase		PRO_0000087995
Sites
	Binding site	7	1	S-adenosyl-L-methionine By similarity
	Binding site	11	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	51	1	S-adenosyl-L-methionine By similarity
	Binding site	179	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21311-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 2F7F5FF2804F4841	FASTA	285	32,255
	10 20 30 40 50 60 MSTILKWAGN KTAIMSELKK HLPAGPRLVE PFAGSCAVMM ATDYPSYLVA DINPDLINLY 70 80 90 100 110 120 KKIAADCEAF ISRARVLFEI ANREVAYYNI RQEFNYSTEI TDFMKAVYFL YLNRHGYRGL 130 140 150 160 170 180 CRYNKSGHFN IPYGNYKNPY FPEKEIRKFA EKAQRATFIC ASFDETLAML QVGDVVYCDP 190 200 210 220 230 240 PYDGTFSGYH TDGFTEDDQY HLASVLEYRS SEGHPVIVSN SDTSLIRSLY RNFTHHYIKA 250 260 270 280 KRSIGVSAGE SKSATEIIAV SGARCWVGFD PSRGVDSSAV YEVRV

References

[1]

"Retron for the 67-base multicopy single-stranded DNA from Escherichia coli: a potential transposable element encoding both reverse transcriptase and Dam methylase functions." Hsu M.-Y., Inouye M., Inouye S. Proc. Natl. Acad. Sci. U.S.A. 87:9454-9458(1990) [PubMed: 1701261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O1:NM / CL-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M55249 Genomic DNA. Translation: AAA23396.1.
PIR	JQ0851.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2846. M.EcoEc67Dam.
Enzyme and pathway databases
BRENDA	2.1.1.72. 246.
Family and domain databases
InterPro	IPR002294. D12N6_MeTrfase. IPR012326. Dam. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMA7_ECOLX

Accession

Primary (citable) accession number: P21311

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	September 22, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)