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P21311 (DMA7_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Retron EC67 DNA adenine methylase

EC=2.1.1.72
Alternative name(s):
M.Eco67Dam
ORF1-EC67 DAM
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
   Biological processDNA replication
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termTransposable element
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

site-specific DNA-methyltransferase (adenine-specific) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Retron EC67 DNA adenine methylase
PRO_0000087995

Sites

Binding site71S-adenosyl-L-methionine By similarity
Binding site111S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site511S-adenosyl-L-methionine By similarity
Binding site1791S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
P21311 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: 2F7F5FF2804F4841

FASTA28532,255
        10         20         30         40         50         60 
MSTILKWAGN KTAIMSELKK HLPAGPRLVE PFAGSCAVMM ATDYPSYLVA DINPDLINLY 

        70         80         90        100        110        120 
KKIAADCEAF ISRARVLFEI ANREVAYYNI RQEFNYSTEI TDFMKAVYFL YLNRHGYRGL 

       130        140        150        160        170        180 
CRYNKSGHFN IPYGNYKNPY FPEKEIRKFA EKAQRATFIC ASFDETLAML QVGDVVYCDP 

       190        200        210        220        230        240 
PYDGTFSGYH TDGFTEDDQY HLASVLEYRS SEGHPVIVSN SDTSLIRSLY RNFTHHYIKA 

       250        260        270        280 
KRSIGVSAGE SKSATEIIAV SGARCWVGFD PSRGVDSSAV YEVRV 

« Hide

References

[1]"Retron for the 67-base multicopy single-stranded DNA from Escherichia coli: a potential transposable element encoding both reverse transcriptase and Dam methylase functions."
Hsu M.-Y., Inouye M., Inouye S.
Proc. Natl. Acad. Sci. U.S.A. 87:9454-9458(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: O1:NM / CL-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55249 Genomic DNA. Translation: AAA23396.1.
PIRJQ0851.

3D structure databases

ProteinModelPortalP21311.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2846. M.EcoEc67Dam.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0338.

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDMA7_ECOLX
AccessionPrimary (citable) accession number: P21311
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries