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P21306

- ATP5E_YEAST

UniProt

P21306 - ATP5E_YEAST

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Protein

ATP synthase subunit epsilon, mitochondrial

Gene
ATP15, YPL271W, P0345
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

GO - Molecular functioni

  1. proton-transporting ATPase activity, rotational mechanism Source: InterPro
  2. proton-transporting ATP synthase activity, rotational mechanism Source: InterPro

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. ATP synthesis coupled proton transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34153-MONOMER.

Protein family/group databases

TCDBi3.A.2.1.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP synthase subunit epsilon, mitochondrial
Short name:
ATPase subunit epsilon
Gene namesi
Name:ATP15
Ordered Locus Names:YPL271W
ORF Names:P0345
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

SGDiS000006192. ATP15.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial proton-transporting ATP synthase, central stalk Source: SGD
  2. proton-transporting ATP synthase complex, catalytic core F(1) Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 6261ATP synthase subunit epsilon, mitochondrialPRO_0000071667Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21306.
PaxDbiP21306.

Expressioni

Gene expression databases

GenevestigatoriP21306.

Interactioni

Subunit structurei

F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c.

Protein-protein interaction databases

BioGridi35942. 58 interactions.
DIPiDIP-3031N.
IntActiP21306. 6 interactions.
MINTiMINT-547186.
STRINGi4932.YPL271W.

Structurei

Secondary structure

1
62
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni4 – 63
Helixi11 – 2414
Helixi28 – 303
Helixi33 – 375
Beta strandi44 – 474

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HLDX-ray2.801/I/R2-62[»]
2WPDX-ray3.43I2-62[»]
3FKSX-ray3.591/I/R2-62[»]
3OE7X-ray3.191/I/R2-62[»]
3OEEX-ray2.741/I/R2-62[»]
3OEHX-ray3.001/I/R2-62[»]
3OFNX-ray3.20I/R2-62[»]
3ZIAX-ray2.50I/S2-62[»]
4B2Qelectron microscopy37.00I/i2-60[»]
ProteinModelPortaliP21306.
SMRiP21306. Positions 2-60.

Miscellaneous databases

EvolutionaryTraceiP21306.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG294695.
HOGENOMiHOG000214506.
KOiK02135.
OMAiYDIGHAL.
OrthoDBiEOG7NSBG1.

Family and domain databases

Gene3Di1.10.1620.20. 1 hit.
InterProiIPR006721. ATPase_F1-cplx_esu_mt.
[Graphical view]
PfamiPF04627. ATP-synt_Eps. 1 hit.
[Graphical view]
SUPFAMiSSF48690. SSF48690. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21306-1 [UniParc]FASTAAdd to Basket

« Hide

MSAWRKAGIS YAAYLNVAAQ AIRSSLKTEL QTASVLNRSQ TDAFYTQYKN   50
GTAASEPTPI TK 62
Length:62
Mass (Da):6,743
Last modified:January 23, 2007 - v2
Checksum:i0CD754A9DEFFD08C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64767 Genomic DNA. Translation: CAA46014.1.
Z73627 Genomic DNA. Translation: CAA98007.1.
AY558140 Genomic DNA. Translation: AAS56466.1.
BK006949 Genomic DNA. Translation: DAA11165.1.
PIRiA45315.
RefSeqiNP_015052.1. NM_001184085.1.

Genome annotation databases

EnsemblFungiiYPL271W; YPL271W; YPL271W.
GeneIDi855857.
KEGGisce:YPL271W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64767 Genomic DNA. Translation: CAA46014.1 .
Z73627 Genomic DNA. Translation: CAA98007.1 .
AY558140 Genomic DNA. Translation: AAS56466.1 .
BK006949 Genomic DNA. Translation: DAA11165.1 .
PIRi A45315.
RefSeqi NP_015052.1. NM_001184085.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HLD X-ray 2.80 1/I/R 2-62 [» ]
2WPD X-ray 3.43 I 2-62 [» ]
3FKS X-ray 3.59 1/I/R 2-62 [» ]
3OE7 X-ray 3.19 1/I/R 2-62 [» ]
3OEE X-ray 2.74 1/I/R 2-62 [» ]
3OEH X-ray 3.00 1/I/R 2-62 [» ]
3OFN X-ray 3.20 I/R 2-62 [» ]
3ZIA X-ray 2.50 I/S 2-62 [» ]
4B2Q electron microscopy 37.00 I/i 2-60 [» ]
ProteinModelPortali P21306.
SMRi P21306. Positions 2-60.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35942. 58 interactions.
DIPi DIP-3031N.
IntActi P21306. 6 interactions.
MINTi MINT-547186.
STRINGi 4932.YPL271W.

Protein family/group databases

TCDBi 3.A.2.1.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

MaxQBi P21306.
PaxDbi P21306.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL271W ; YPL271W ; YPL271W .
GeneIDi 855857.
KEGGi sce:YPL271W.

Organism-specific databases

SGDi S000006192. ATP15.

Phylogenomic databases

eggNOGi NOG294695.
HOGENOMi HOG000214506.
KOi K02135.
OMAi YDIGHAL.
OrthoDBi EOG7NSBG1.

Enzyme and pathway databases

BioCyci YEAST:G3O-34153-MONOMER.

Miscellaneous databases

EvolutionaryTracei P21306.
NextBioi 980466.

Gene expression databases

Genevestigatori P21306.

Family and domain databases

Gene3Di 1.10.1620.20. 1 hit.
InterProi IPR006721. ATPase_F1-cplx_esu_mt.
[Graphical view ]
Pfami PF04627. ATP-synt_Eps. 1 hit.
[Graphical view ]
SUPFAMi SSF48690. SSF48690. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ATP synthase of yeast mitochondria. Isolation and disruption of the ATP epsilon gene."
    Guelin E., Chevallier J., Rigoulet M., Guerin B., Velours J.
    J. Biol. Chem. 268:161-167(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D273-10B/A/H/U.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Isolation and complete amino acid sequence of the mitochondrial ATP synthase epsilon-subunit of the yeast Saccharomyces cerevisiae."
    Arselin G., Gandar J.-C., Guerin B., Velours J.
    J. Biol. Chem. 266:723-727(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-62.
    Strain: ATCC 60782 / S / NCYC 232 / American yeast foam.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiATP5E_YEAST
AccessioniPrimary (citable) accession number: P21306
Secondary accession number(s): D6W399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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