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P21306

- ATP5E_YEAST

UniProt

P21306 - ATP5E_YEAST

Protein

ATP synthase subunit epsilon, mitochondrial

Gene

ATP15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

    GO - Molecular functioni

    1. proton-transporting ATPase activity, rotational mechanism Source: InterPro
    2. proton-transporting ATP synthase activity, rotational mechanism Source: InterPro

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. ATP synthesis coupled proton transport Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    ATP synthesis, Hydrogen ion transport, Ion transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34153-MONOMER.

    Protein family/group databases

    TCDBi3.A.2.1.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP synthase subunit epsilon, mitochondrial
    Short name:
    ATPase subunit epsilon
    Gene namesi
    Name:ATP15
    Ordered Locus Names:YPL271W
    ORF Names:P0345
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    SGDiS000006192. ATP15.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial proton-transporting ATP synthase, central stalk Source: SGD
    2. proton-transporting ATP synthase complex, catalytic core F(1) Source: SGD

    Keywords - Cellular componenti

    CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 6261ATP synthase subunit epsilon, mitochondrialPRO_0000071667Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei52 – 521Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP21306.
    PaxDbiP21306.

    Expressioni

    Gene expression databases

    GenevestigatoriP21306.

    Interactioni

    Subunit structurei

    F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c.

    Protein-protein interaction databases

    BioGridi35942. 58 interactions.
    DIPiDIP-3031N.
    IntActiP21306. 6 interactions.
    MINTiMINT-547186.
    STRINGi4932.YPL271W.

    Structurei

    Secondary structure

    1
    62
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni4 – 63
    Helixi11 – 2414
    Helixi28 – 303
    Helixi33 – 375
    Beta strandi44 – 474

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HLDX-ray2.801/I/R2-62[»]
    2WPDX-ray3.43I2-62[»]
    3FKSX-ray3.591/I/R2-62[»]
    3OE7X-ray3.191/I/R2-62[»]
    3OEEX-ray2.741/I/R2-62[»]
    3OEHX-ray3.001/I/R2-62[»]
    3OFNX-ray3.20I/R2-62[»]
    3ZIAX-ray2.50I/S2-62[»]
    4B2Qelectron microscopy37.00I/i2-60[»]
    ProteinModelPortaliP21306.
    SMRiP21306. Positions 2-60.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21306.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic ATPase epsilon family.Curated

    Phylogenomic databases

    eggNOGiNOG294695.
    HOGENOMiHOG000214506.
    KOiK02135.
    OMAiYDIGHAL.
    OrthoDBiEOG7NSBG1.

    Family and domain databases

    Gene3Di1.10.1620.20. 1 hit.
    InterProiIPR006721. ATPase_F1-cplx_esu_mt.
    [Graphical view]
    PfamiPF04627. ATP-synt_Eps. 1 hit.
    [Graphical view]
    SUPFAMiSSF48690. SSF48690. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21306-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAWRKAGIS YAAYLNVAAQ AIRSSLKTEL QTASVLNRSQ TDAFYTQYKN   50
    GTAASEPTPI TK 62
    Length:62
    Mass (Da):6,743
    Last modified:January 23, 2007 - v2
    Checksum:i0CD754A9DEFFD08C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64767 Genomic DNA. Translation: CAA46014.1.
    Z73627 Genomic DNA. Translation: CAA98007.1.
    AY558140 Genomic DNA. Translation: AAS56466.1.
    BK006949 Genomic DNA. Translation: DAA11165.1.
    PIRiA45315.
    RefSeqiNP_015052.1. NM_001184085.1.

    Genome annotation databases

    EnsemblFungiiYPL271W; YPL271W; YPL271W.
    GeneIDi855857.
    KEGGisce:YPL271W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64767 Genomic DNA. Translation: CAA46014.1 .
    Z73627 Genomic DNA. Translation: CAA98007.1 .
    AY558140 Genomic DNA. Translation: AAS56466.1 .
    BK006949 Genomic DNA. Translation: DAA11165.1 .
    PIRi A45315.
    RefSeqi NP_015052.1. NM_001184085.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HLD X-ray 2.80 1/I/R 2-62 [» ]
    2WPD X-ray 3.43 I 2-62 [» ]
    3FKS X-ray 3.59 1/I/R 2-62 [» ]
    3OE7 X-ray 3.19 1/I/R 2-62 [» ]
    3OEE X-ray 2.74 1/I/R 2-62 [» ]
    3OEH X-ray 3.00 1/I/R 2-62 [» ]
    3OFN X-ray 3.20 I/R 2-62 [» ]
    3ZIA X-ray 2.50 I/S 2-62 [» ]
    4B2Q electron microscopy 37.00 I/i 2-60 [» ]
    ProteinModelPortali P21306.
    SMRi P21306. Positions 2-60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35942. 58 interactions.
    DIPi DIP-3031N.
    IntActi P21306. 6 interactions.
    MINTi MINT-547186.
    STRINGi 4932.YPL271W.

    Protein family/group databases

    TCDBi 3.A.2.1.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Proteomic databases

    MaxQBi P21306.
    PaxDbi P21306.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL271W ; YPL271W ; YPL271W .
    GeneIDi 855857.
    KEGGi sce:YPL271W.

    Organism-specific databases

    SGDi S000006192. ATP15.

    Phylogenomic databases

    eggNOGi NOG294695.
    HOGENOMi HOG000214506.
    KOi K02135.
    OMAi YDIGHAL.
    OrthoDBi EOG7NSBG1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34153-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P21306.
    NextBioi 980466.

    Gene expression databases

    Genevestigatori P21306.

    Family and domain databases

    Gene3Di 1.10.1620.20. 1 hit.
    InterProi IPR006721. ATPase_F1-cplx_esu_mt.
    [Graphical view ]
    Pfami PF04627. ATP-synt_Eps. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48690. SSF48690. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "ATP synthase of yeast mitochondria. Isolation and disruption of the ATP epsilon gene."
      Guelin E., Chevallier J., Rigoulet M., Guerin B., Velours J.
      J. Biol. Chem. 268:161-167(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: D273-10B/A/H/U.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Isolation and complete amino acid sequence of the mitochondrial ATP synthase epsilon-subunit of the yeast Saccharomyces cerevisiae."
      Arselin G., Gandar J.-C., Guerin B., Velours J.
      J. Biol. Chem. 266:723-727(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-62.
      Strain: ATCC 60782 / S / NCYC 232 / American yeast foam.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
      Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
      Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiATP5E_YEAST
    AccessioniPrimary (citable) accession number: P21306
    Secondary accession number(s): D6W399
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4280 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3