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Protein

Thymidine kinase

Gene

TK

Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei243 – 2431Proton acceptorUniRule annotation
Binding sitei260 – 2601SubstrateUniRule annotation
Binding sitei281 – 2811SubstrateUniRule annotation
Binding sitei368 – 3681ATPUniRule annotation
Binding sitei374 – 3741SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi216 – 2238ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
Ordered Locus Names:21
OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10383 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
Proteomesi
  • UP000000587 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Thymidine kinasePRO_0000175077Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP21293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P21293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGRGQPPKK NDTYDYPRKQ PPKNGSYDNY DYPTSTKTRS TNKQRKDSNY
60 70 80 90 100
PPRETIFEPD LAADPIYSVP RPPSRVPHKL VKVNYKSNLV PITASNSVSE
110 120 130 140 150
LLSLHDETQV TECVEAPLIA KSPDITVYEK MFSVRPKHTL TKLEGKQKMF
160 170 180 190 200
TRKKKGSFVK IGSNMLEFGE SLKSKLHNDS KKSPDEPDGL VHVPVHLLYP
210 220 230 240 250
PKHQDPVPAF FIFLEGSIGV GKTTLLKSMN GILGGKNVLA FHEPIAYWTD
260 270 280 290 300
VFSNSLEEVY KLTLPAKVGR TSNSAKLLAC QLKFASPLLA LKTATDRLSS
310 320 330 340 350
PKNSLLSSDM WVMFDRHPLS ATVVFPYMHF QNGFLSFSHL IQLWSSFKAS
360 370 380 390 400
RGDNIILLNL NSQENLKRVK KRNRKEEKSV SIEHIRLLNN CYHAVYCAWL
410 420 430 440 450
LVQNFTPEEI VEVCFNAKHI TDLSSSKPSF LAKHVSTEDM LKSSIFNTWI
460 470 480 490 500
EMTKAHRDSC TLMECLLTFC KELEKVQLIH VNVSPFTDDI PGLWASIYTS
510 520
IRRNSAIKPN RVNWLALEDL ARTFNSQ
Length:527
Mass (Da):59,807
Last modified:May 1, 1991 - v1
Checksum:i4E8E3A3A178FAD7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA. Translation: CAA45643.1.
D00543 Genomic DNA. Translation: BAA00432.1.
RefSeqiNP_040223.1. NC_001350.1.

Genome annotation databases

GeneIDi1488261.
KEGGivg:1488261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA. Translation: CAA45643.1.
D00543 Genomic DNA. Translation: BAA00432.1.
RefSeqiNP_040223.1. NC_001350.1.

3D structure databases

ProteinModelPortaliP21293.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1488261.
KEGGivg:1488261.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A comparative analysis of the sequence of the thymidine kinase gene of a gammaherpesvirus, herpesvirus saimiri."
    Honess R.W., Craxton M.A., Williams L., Gompels U.A.
    J. Gen. Virol. 70:3003-3013(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Primary structure of the herpesvirus saimiri genome."
    Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
    J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKITH_SHV21
AccessioniPrimary (citable) accession number: P21293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: July 6, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.