Reviewed,
UniProtKB/Swiss-Prot P21281 (VATB2_HUMAN)
Last modified
November 3, 2009.
Version 103.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: V-type proton ATPase subunit B, brain isoform Short name=V-ATPase subunit B 2 Alternative name(s): Vacuolar proton pump subunit B 2 Endomembrane proton pump 58 kDa subunit HO57 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 511 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. |
| Subunit structure | V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein). |
| Subcellular location | Endomembrane system; Peripheral membrane protein. Melanosome. Note: Endomembrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.6 Ref.7 |
| Sequence similarities | Belongs to the ATPase alpha/beta chains family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 511 | 511 | V-type proton ATPase subunit B, brain isoform | PRO_0000144626 | |||||
Amino acid modifications | |||||||||
| Modified residue | 404 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 498 | 1 | Phosphoserine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 28 | 1 | A → S in CAA44721. Ref.1 | ||||||
| Sequence conflict | 29 | 1 | R → Q in AAH30640. Ref.3 | ||||||
| Sequence conflict | 171 | 1 | Q → R in AAH30640. Ref.3 | ||||||
| Sequence conflict | 342 | 1 | E → G in AAH30640. Ref.3 | ||||||
| Sequence conflict | 376 | 1 | Q → L in AAA58661. Ref.2 | ||||||
| Sequence conflict | 424 – 425 | 2 | AC → RA in AAA35610. Ref.5 | ||||||
| Sequence conflict | 435 | 1 | M → V in AAA35610. Ref.5 | ||||||
| Sequence conflict | 510 – 511 | 2 | KH → ND in AAA35610. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Selectively amplified expression of an isoform of the vacuolar H(+)-ATPase 56-kilodalton subunit in renal intercalated cells." Nelson R.D., Guo X.-L., Masood K., Brown D., Kalkbrenner M., Gluck S. Proc. Natl. Acad. Sci. U.S.A. 89:3541-3545(1992) [PubMed: 1373501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney. |
| [2] | "Heterogeneity of vacuolar H(+)-ATPase: differential expression of two human subunit B isoforms." van Hille B., Richener H., Schmid P., Puettner I., Green J.R., Bilbe G. Biochem. J. 303:191-198(1994) [PubMed: 7945239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Eye. |
| [4] | "Transcriptional regulation of the vacuolar H(+)-ATPase B2 subunit gene in differentiating THP-1 cells." Lee B.S., Underhill D.M., Crane M.K., Gluck S.L. J. Biol. Chem. 270:7320-7329(1995) [PubMed: 7706273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45. |
| [5] | "An mRNA from human brain encodes an isoform of the B subunit of the vacuolar H(+)-ATPase." Bernasconi P., Rausch T., Struve I., Morgan L., Taiz L. J. Biol. Chem. 265:17428-17431(1990) [PubMed: 2145275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 106-511. Tissue: Brain. |
| [6] | "Proteomic analysis of early melanosomes: identification of novel melanosomal proteins." Basrur V., Yang F., Kushimoto T., Higashimoto Y., Yasumoto K., Valencia J., Muller J., Vieira W.D., Watabe H., Shabanowitz J., Hearing V.J., Hunt D.F., Appella E. J. Proteome Res. 2:69-79(2003) [PubMed: 12643545] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [7] | "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F. J. Proteome Res. 5:3135-3144(2006) [PubMed: 17081065] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| M60346 mRNA. Translation: AAA35610.1. L35249 mRNA. Translation: AAA58661.1. BC003100 mRNA. Translation: AAH03100.1. BC007309 mRNA. Translation: AAH07309.1. BC030640 mRNA. Translation: AAH30640.1. Z37165 Genomic DNA. Translation: CAA85522.1. X62949 mRNA. Translation: CAA44721.1. | |
| IPI | IPI00007812. |
| PIR | B44138. I39208. |
| RefSeq | NP_001684.2. |
| UniGene | Hs.295917 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P21281. |
Protein family/group databases | |
| TCDB | 3.A.2.2.4. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily. |
PTM databases | |
| PhosphoSite | P21281. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | IPI00007812. |
Proteomic databases | |
| PeptideAtlas | P21281. |
| PRIDE | P21281. |
Genome annotation databases | |
| Ensembl | ENST00000276390; ENSP00000276390; ENSG00000147416; Homo sapiens. [Genome view] |
| GeneID | 526. |
| KEGG | hsa:526. |
| UCSC | uc003wzp.1. human. |
Organism-specific databases | |
| CTD | 526. |
| GeneCards | GC08P020098. |
| H-InvDB | HIX0007352. HIX0068099. |
| HGNC | HGNC:854. ATP6V1B2. |
| HPA | HPA008147. |
| MIM | 606939. gene. |
| PharmGKB | PA25155. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P21281. |
| HOVERGEN | P21281. |
| OMA | EGQIFVD. |
Enzyme and pathway databases | |
| BRENDA | 3.6.3.14. 247. |
Gene expression databases | |
| ArrayExpress | P21281. |
| Bgee | P21281. |
| CleanEx | HS_ATP6V1B2. |
| Genevestigator | P21281. |
| GermOnline | ENSG00000147416. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C. IPR004100. ATPase_F1/V1/A1-cplx_a/bsu_N. IPR020003. ATPase_F1/V1/A1_a/bsu_AS. IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd. IPR005723. ATPase_V1-cplx_bsu. [Graphical view] |
| Pfam | PF00006. ATP-synt_ab. 1 hit. PF00306. ATP-synt_ab_C. 1 hit. PF02874. ATP-synt_ab_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01040. V-ATPase_V1_B. 1 hit. |
| PROSITE | PS00152. ATPASE_ALPHA_BETA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 2183. |
| SOURCE | Search... |
Entry information
| Entry name | VATB2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P21281 Secondary accession number(s): Q14544, Q15859, Q96IR0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 8 Human chromosome 8: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


