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P21281

- VATB2_HUMAN

UniProt

P21281 - VATB2_HUMAN

Protein

V-type proton ATPase subunit B, brain isoform

Gene

ATP6V1B2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 155 (01 Oct 2014)
      Sequence version 3 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. hydrogen ion transmembrane transporter activity Source: ProtInc
    3. proton-transporting ATPase activity, rotational mechanism Source: ProtInc

    GO - Biological processi

    1. ATP hydrolysis coupled proton transport Source: InterPro
    2. cellular iron ion homeostasis Source: Reactome
    3. insulin receptor signaling pathway Source: Reactome
    4. interaction with host Source: Reactome
    5. phagosome maturation Source: Reactome
    6. proton transport Source: ProtInc
    7. transferrin transport Source: Reactome
    8. transmembrane transport Source: Reactome

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Hydrogen ion transport, Ion transport, Transport

    Enzyme and pathway databases

    BioCyciMetaCyc:HS07429-MONOMER.
    ReactomeiREACT_1109. Insulin receptor recycling.
    REACT_121256. Phagosomal maturation (early endosomal stage).
    REACT_25283. Transferrin endocytosis and recycling.

    Protein family/group databases

    TCDBi3.A.2.2.4. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    V-type proton ATPase subunit B, brain isoform
    Short name:
    V-ATPase subunit B 2
    Alternative name(s):
    Endomembrane proton pump 58 kDa subunit
    HO57
    Vacuolar proton pump subunit B 2
    Gene namesi
    Name:ATP6V1B2
    Synonyms:ATP6B2, VPP3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:854. ATP6V1B2.

    Subcellular locationi

    Endomembrane system; Peripheral membrane protein. Melanosome
    Note: Endomembrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. endomembrane system Source: UniProtKB-SubCell
    3. extracellular vesicular exosome Source: UniProt
    4. integral component of membrane Source: Ensembl
    5. intracellular membrane-bounded organelle Source: HPA
    6. lysosomal membrane Source: UniProtKB
    7. melanosome Source: UniProtKB-SubCell
    8. microvillus Source: Ensembl
    9. plasma membrane Source: UniProtKB
    10. proton-transporting V-type ATPase, V1 domain Source: InterPro

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25155.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 511511V-type proton ATPase subunit B, brain isoformPRO_0000144626Add
    BLAST

    Proteomic databases

    MaxQBiP21281.
    PaxDbiP21281.
    PeptideAtlasiP21281.
    PRIDEiP21281.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00007812.

    PTM databases

    PhosphoSiteiP21281.

    Expressioni

    Gene expression databases

    ArrayExpressiP21281.
    BgeeiP21281.
    CleanExiHS_ATP6V1B2.
    GenevestigatoriP21281.

    Organism-specific databases

    HPAiHPA008147.

    Interactioni

    Subunit structurei

    V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein).

    Protein-protein interaction databases

    BioGridi107009. 65 interactions.
    IntActiP21281. 4 interactions.
    MINTiMINT-5004128.
    STRINGi9606.ENSP00000276390.

    Structurei

    3D structure databases

    ProteinModelPortaliP21281.
    SMRiP21281. Positions 55-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATPase alpha/beta chains family.Curated

    Phylogenomic databases

    eggNOGiCOG1156.
    HOGENOMiHOG000165320.
    HOVERGENiHBG002176.
    InParanoidiP21281.
    KOiK02147.
    OMAiTVCEFTG.
    OrthoDBiEOG7NW68Q.
    PhylomeDBiP21281.
    TreeFamiTF300313.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00310. ATP_synth_B_arch.
    InterProiIPR020003. ATPase_a/bsu_AS.
    IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR004100. ATPase_F1_a/bsu_N.
    IPR005723. ATPase_V1-cplx_bsu.
    IPR027417. P-loop_NTPase.
    IPR022879. V-ATPase_su_B/beta.
    [Graphical view]
    PfamiPF00006. ATP-synt_ab. 1 hit.
    PF00306. ATP-synt_ab_C. 1 hit.
    PF02874. ATP-synt_ab_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR01040. V-ATPase_V1_B. 1 hit.
    PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21281-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALRAMRGIV NGAAPELPVP TGGPAVGARE QALAVSRNYL SQPRLTYKTV    50
    SGVNGPLVIL DHVKFPRYAE IVHLTLPDGT KRSGQVLEVS GSKAVVQVFE 100
    GTSGIDAKKT SCEFTGDILR TPVSEDMLGR VFNGSGKPID RGPVVLAEDF 150
    LDIMGQPINP QCRIYPEEMI QTGISAIDGM NSIARGQKIP IFSAAGLPHN 200
    EIAAQICRQA GLVKKSKDVV DYSEENFAIV FAAMGVNMET ARFFKSDFEE 250
    NGSMDNVCLF LNLANDPTIE RIITPRLALT TAEFLAYQCE KHVLVILTDM 300
    SSYAEALREV SAAREEVPGR RGFPGYMYTD LATIYERAGR VEGRNGSITQ 350
    IPILTMPNDD ITHPIPDLTG YITEGQIYVD RQLHNRQIYP PINVLPSLSR 400
    LMKSAIGEGM TRKDHADVSN QLYACYAIGK DVQAMKAVVG EEALTSDDLL 450
    YLEFLQKFER NFIAQGPYEN RTVFETLDIG WQLLRIFPKE MLKRIPQSTL 500
    SEFYPRDSAK H 511
    Length:511
    Mass (Da):56,501
    Last modified:December 1, 2000 - v3
    Checksum:iE01E85BBA36E5DED
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281A → S in CAA44721. (PubMed:1373501)Curated
    Sequence conflicti29 – 291R → Q in AAH30640. (PubMed:15489334)Curated
    Sequence conflicti171 – 1711Q → R in AAH30640. (PubMed:15489334)Curated
    Sequence conflicti342 – 3421E → G in AAH30640. (PubMed:15489334)Curated
    Sequence conflicti376 – 3761Q → L in AAA58661. (PubMed:7945239)Curated
    Sequence conflicti424 – 4252AC → RA in AAA35610. (PubMed:2145275)Curated
    Sequence conflicti435 – 4351M → V in AAA35610. (PubMed:2145275)Curated
    Sequence conflicti510 – 5112KH → ND in AAA35610. (PubMed:2145275)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60346 mRNA. Translation: AAA35610.1.
    L35249 mRNA. Translation: AAA58661.1.
    AK312372 mRNA. Translation: BAG35290.1.
    CH471080 Genomic DNA. Translation: EAW63758.1.
    CH471080 Genomic DNA. Translation: EAW63759.1.
    BC003100 mRNA. Translation: AAH03100.1.
    BC007309 mRNA. Translation: AAH07309.1.
    BC030640 mRNA. Translation: AAH30640.1.
    Z37165 Genomic DNA. Translation: CAA85522.1.
    X62949 mRNA. Translation: CAA44721.1.
    CCDSiCCDS6014.1.
    PIRiB44138.
    I39208.
    RefSeqiNP_001684.2. NM_001693.3.
    UniGeneiHs.295917.

    Genome annotation databases

    EnsembliENST00000276390; ENSP00000276390; ENSG00000147416.
    GeneIDi526.
    KEGGihsa:526.
    UCSCiuc003wzp.3. human.

    Polymorphism databases

    DMDMi12643271.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60346 mRNA. Translation: AAA35610.1 .
    L35249 mRNA. Translation: AAA58661.1 .
    AK312372 mRNA. Translation: BAG35290.1 .
    CH471080 Genomic DNA. Translation: EAW63758.1 .
    CH471080 Genomic DNA. Translation: EAW63759.1 .
    BC003100 mRNA. Translation: AAH03100.1 .
    BC007309 mRNA. Translation: AAH07309.1 .
    BC030640 mRNA. Translation: AAH30640.1 .
    Z37165 Genomic DNA. Translation: CAA85522.1 .
    X62949 mRNA. Translation: CAA44721.1 .
    CCDSi CCDS6014.1.
    PIRi B44138.
    I39208.
    RefSeqi NP_001684.2. NM_001693.3.
    UniGenei Hs.295917.

    3D structure databases

    ProteinModelPortali P21281.
    SMRi P21281. Positions 55-505.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107009. 65 interactions.
    IntActi P21281. 4 interactions.
    MINTi MINT-5004128.
    STRINGi 9606.ENSP00000276390.

    Chemistry

    BindingDBi P21281.
    ChEMBLi CHEMBL5641.
    DrugBanki DB05260. Gallium nitrate.

    Protein family/group databases

    TCDBi 3.A.2.2.4. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    PTM databases

    PhosphoSitei P21281.

    Polymorphism databases

    DMDMi 12643271.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00007812.

    Proteomic databases

    MaxQBi P21281.
    PaxDbi P21281.
    PeptideAtlasi P21281.
    PRIDEi P21281.

    Protocols and materials databases

    DNASUi 526.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000276390 ; ENSP00000276390 ; ENSG00000147416 .
    GeneIDi 526.
    KEGGi hsa:526.
    UCSCi uc003wzp.3. human.

    Organism-specific databases

    CTDi 526.
    GeneCardsi GC08P020054.
    HGNCi HGNC:854. ATP6V1B2.
    HPAi HPA008147.
    MIMi 606939. gene.
    neXtProti NX_P21281.
    PharmGKBi PA25155.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1156.
    HOGENOMi HOG000165320.
    HOVERGENi HBG002176.
    InParanoidi P21281.
    KOi K02147.
    OMAi TVCEFTG.
    OrthoDBi EOG7NW68Q.
    PhylomeDBi P21281.
    TreeFami TF300313.

    Enzyme and pathway databases

    BioCyci MetaCyc:HS07429-MONOMER.
    Reactomei REACT_1109. Insulin receptor recycling.
    REACT_121256. Phagosomal maturation (early endosomal stage).
    REACT_25283. Transferrin endocytosis and recycling.

    Miscellaneous databases

    GeneWikii ATP6V1B2.
    GenomeRNAii 526.
    NextBioi 2183.
    PROi P21281.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P21281.
    Bgeei P21281.
    CleanExi HS_ATP6V1B2.
    Genevestigatori P21281.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00310. ATP_synth_B_arch.
    InterProi IPR020003. ATPase_a/bsu_AS.
    IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR004100. ATPase_F1_a/bsu_N.
    IPR005723. ATPase_V1-cplx_bsu.
    IPR027417. P-loop_NTPase.
    IPR022879. V-ATPase_su_B/beta.
    [Graphical view ]
    Pfami PF00006. ATP-synt_ab. 1 hit.
    PF00306. ATP-synt_ab_C. 1 hit.
    PF02874. ATP-synt_ab_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR01040. V-ATPase_V1_B. 1 hit.
    PROSITEi PS00152. ATPASE_ALPHA_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Selectively amplified expression of an isoform of the vacuolar H(+)-ATPase 56-kilodalton subunit in renal intercalated cells."
      Nelson R.D., Guo X.-L., Masood K., Brown D., Kalkbrenner M., Gluck S.
      Proc. Natl. Acad. Sci. U.S.A. 89:3541-3545(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. "Heterogeneity of vacuolar H(+)-ATPase: differential expression of two human subunit B isoforms."
      van Hille B., Richener H., Schmid P., Puettner I., Green J.R., Bilbe G.
      Biochem. J. 303:191-198(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Amygdala.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Eye.
    6. "Transcriptional regulation of the vacuolar H(+)-ATPase B2 subunit gene in differentiating THP-1 cells."
      Lee B.S., Underhill D.M., Crane M.K., Gluck S.L.
      J. Biol. Chem. 270:7320-7329(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
    7. "An mRNA from human brain encodes an isoform of the B subunit of the vacuolar H(+)-ATPase."
      Bernasconi P., Rausch T., Struve I., Morgan L., Taiz L.
      J. Biol. Chem. 265:17428-17431(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 106-511.
      Tissue: Brain.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Tissue: Melanoma.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Tissue: Melanoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVATB2_HUMAN
    AccessioniPrimary (citable) accession number: P21281
    Secondary accession number(s): B2R5Z3
    , D3DSQ5, Q14544, Q15859, Q96IR0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 155 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3