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P21281 (VATB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
V-type proton ATPase subunit B, brain isoform

Short name=V-ATPase subunit B 2
Alternative name(s):
Endomembrane proton pump 58 kDa subunit
HO57
Vacuolar proton pump subunit B 2
Gene names
Name:ATP6V1B2
Synonyms:ATP6B2, VPP3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. HAMAP-Rule MF_00310

Subunit structure

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein).

Subcellular location

Endomembrane system; Peripheral membrane protein. Melanosome. Note: Endomembrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.8 Ref.9

Sequence similarities

Belongs to the ATPase alpha/beta chains family.

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentMembrane
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP hydrolysis coupled proton transport

Inferred from electronic annotation. Source: InterPro

cellular iron ion homeostasis

Traceable author statement. Source: Reactome

insulin receptor signaling pathway

Traceable author statement. Source: Reactome

interaction with host

Traceable author statement. Source: Reactome

phagosome maturation

Traceable author statement. Source: Reactome

proton transport

Traceable author statement Ref.7. Source: ProtInc

transferrin transport

Traceable author statement. Source: Reactome

transmembrane transport

Traceable author statement. Source: Reactome

   Cellular_componentcytosol

Inferred from sequence or structural similarity. Source: UniProtKB

endomembrane system

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867PubMed 19199708PubMed 23376485. Source: UniProt

integral component of membrane

Inferred from electronic annotation. Source: Ensembl

intracellular membrane-bounded organelle

Inferred from direct assay. Source: HPA

lysosomal membrane

Inferred from direct assay PubMed 17897319. Source: UniProtKB

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

microvillus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

proton-transporting V-type ATPase, V1 domain

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

hydrogen ion transmembrane transporter activity

Traceable author statement Ref.7. Source: ProtInc

proton-transporting ATPase activity, rotational mechanism

Traceable author statement Ref.7. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 511511V-type proton ATPase subunit B, brain isoform HAMAP-Rule MF_00310
PRO_0000144626

Experimental info

Sequence conflict281A → S in CAA44721. Ref.1
Sequence conflict291R → Q in AAH30640. Ref.5
Sequence conflict1711Q → R in AAH30640. Ref.5
Sequence conflict3421E → G in AAH30640. Ref.5
Sequence conflict3761Q → L in AAA58661. Ref.2
Sequence conflict424 – 4252AC → RA in AAA35610. Ref.7
Sequence conflict4351M → V in AAA35610. Ref.7
Sequence conflict510 – 5112KH → ND in AAA35610. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P21281 [UniParc].

Last modified December 1, 2000. Version 3.
Checksum: E01E85BBA36E5DED

FASTA51156,501
        10         20         30         40         50         60 
MALRAMRGIV NGAAPELPVP TGGPAVGARE QALAVSRNYL SQPRLTYKTV SGVNGPLVIL 

        70         80         90        100        110        120 
DHVKFPRYAE IVHLTLPDGT KRSGQVLEVS GSKAVVQVFE GTSGIDAKKT SCEFTGDILR 

       130        140        150        160        170        180 
TPVSEDMLGR VFNGSGKPID RGPVVLAEDF LDIMGQPINP QCRIYPEEMI QTGISAIDGM 

       190        200        210        220        230        240 
NSIARGQKIP IFSAAGLPHN EIAAQICRQA GLVKKSKDVV DYSEENFAIV FAAMGVNMET 

       250        260        270        280        290        300 
ARFFKSDFEE NGSMDNVCLF LNLANDPTIE RIITPRLALT TAEFLAYQCE KHVLVILTDM 

       310        320        330        340        350        360 
SSYAEALREV SAAREEVPGR RGFPGYMYTD LATIYERAGR VEGRNGSITQ IPILTMPNDD 

       370        380        390        400        410        420 
ITHPIPDLTG YITEGQIYVD RQLHNRQIYP PINVLPSLSR LMKSAIGEGM TRKDHADVSN 

       430        440        450        460        470        480 
QLYACYAIGK DVQAMKAVVG EEALTSDDLL YLEFLQKFER NFIAQGPYEN RTVFETLDIG 

       490        500        510 
WQLLRIFPKE MLKRIPQSTL SEFYPRDSAK H 

« Hide

References

« Hide 'large scale' references
[1]"Selectively amplified expression of an isoform of the vacuolar H(+)-ATPase 56-kilodalton subunit in renal intercalated cells."
Nelson R.D., Guo X.-L., Masood K., Brown D., Kalkbrenner M., Gluck S.
Proc. Natl. Acad. Sci. U.S.A. 89:3541-3545(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[2]"Heterogeneity of vacuolar H(+)-ATPase: differential expression of two human subunit B isoforms."
van Hille B., Richener H., Schmid P., Puettner I., Green J.R., Bilbe G.
Biochem. J. 303:191-198(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Eye.
[6]"Transcriptional regulation of the vacuolar H(+)-ATPase B2 subunit gene in differentiating THP-1 cells."
Lee B.S., Underhill D.M., Crane M.K., Gluck S.L.
J. Biol. Chem. 270:7320-7329(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
[7]"An mRNA from human brain encodes an isoform of the B subunit of the vacuolar H(+)-ATPase."
Bernasconi P., Rausch T., Struve I., Morgan L., Taiz L.
J. Biol. Chem. 265:17428-17431(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 106-511.
Tissue: Brain.
[8]"Proteomic analysis of early melanosomes: identification of novel melanosomal proteins."
Basrur V., Yang F., Kushimoto T., Higashimoto Y., Yasumoto K., Valencia J., Muller J., Vieira W.D., Watabe H., Shabanowitz J., Hearing V.J., Hunt D.F., Appella E.
J. Proteome Res. 2:69-79(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Melanoma.
[9]"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F.
J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Melanoma.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60346 mRNA. Translation: AAA35610.1.
L35249 mRNA. Translation: AAA58661.1.
AK312372 mRNA. Translation: BAG35290.1.
CH471080 Genomic DNA. Translation: EAW63758.1.
CH471080 Genomic DNA. Translation: EAW63759.1.
BC003100 mRNA. Translation: AAH03100.1.
BC007309 mRNA. Translation: AAH07309.1.
BC030640 mRNA. Translation: AAH30640.1.
Z37165 Genomic DNA. Translation: CAA85522.1.
X62949 mRNA. Translation: CAA44721.1.
CCDSCCDS6014.1.
PIRB44138.
I39208.
RefSeqNP_001684.2. NM_001693.3.
UniGeneHs.295917.

3D structure databases

ProteinModelPortalP21281.
SMRP21281. Positions 55-505.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107009. 65 interactions.
IntActP21281. 4 interactions.
MINTMINT-5004128.
STRING9606.ENSP00000276390.

Chemistry

BindingDBP21281.
ChEMBLCHEMBL5641.

Protein family/group databases

TCDB3.A.2.2.4. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

PhosphoSiteP21281.

Polymorphism databases

DMDM12643271.

2D gel databases

REPRODUCTION-2DPAGEIPI00007812.

Proteomic databases

MaxQBP21281.
PaxDbP21281.
PeptideAtlasP21281.
PRIDEP21281.

Protocols and materials databases

DNASU526.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000276390; ENSP00000276390; ENSG00000147416.
GeneID526.
KEGGhsa:526.
UCSCuc003wzp.3. human.

Organism-specific databases

CTD526.
GeneCardsGC08P020054.
HGNCHGNC:854. ATP6V1B2.
HPAHPA008147.
MIM606939. gene.
neXtProtNX_P21281.
PharmGKBPA25155.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1156.
HOGENOMHOG000165320.
HOVERGENHBG002176.
InParanoidP21281.
KOK02147.
OMATVCEFTG.
OrthoDBEOG7NW68Q.
PhylomeDBP21281.
TreeFamTF300313.

Enzyme and pathway databases

BioCycMetaCyc:HS07429-MONOMER.
ReactomeREACT_111102. Signal Transduction.
REACT_116125. Disease.
REACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressP21281.
BgeeP21281.
CleanExHS_ATP6V1B2.
GenevestigatorP21281.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_00310. ATP_synth_B_arch.
InterProIPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR005723. ATPase_V1-cplx_bsu.
IPR027417. P-loop_NTPase.
IPR022879. V-ATPase_su_B/beta.
[Graphical view]
PfamPF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR01040. V-ATPase_V1_B. 1 hit.
PROSITEPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiATP6V1B2.
GenomeRNAi526.
NextBio2183.
PROP21281.
SOURCESearch...

Entry information

Entry nameVATB2_HUMAN
AccessionPrimary (citable) accession number: P21281
Secondary accession number(s): B2R5Z3 expand/collapse secondary AC list , D3DSQ5, Q14544, Q15859, Q96IR0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM