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Protein

CCA tRNA nucleotidyltransferase, mitochondrial

Gene

CCA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site.

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.

GO - Molecular functioni

  • ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: SGD
  • CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
  • RNA binding Source: UniProtKB-KW
  • tRNA adenylyltransferase activity Source: SGD

GO - Biological processi

  • tRNA 3'-terminal CCA addition Source: SGD

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30329-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA tRNA nucleotidyltransferase, mitochondrial (EC:2.7.7.72)
Alternative name(s):
CCA-adding enzyme
tRNA CCA-pyrophosphorylase
tRNA adenylyltransferase
tRNA nucleotidyltransferase
Gene namesi
Name:CCA1
Synonyms:TNT1
Ordered Locus Names:YER168C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER168C.
SGDiS000000970. CCA1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000004779? – 546CCA tRNA nucleotidyltransferase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PeptideAtlasiP21269.
PRIDEiP21269.

PTM databases

iPTMnetiP21269.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ENO2P009252EBI-4347,EBI-6475

Protein-protein interaction databases

BioGridi36921. 259 interactors.
DIPiDIP-2841N.
IntActiP21269. 6 interactors.
MINTiMINT-633371.
STRINGi4932.YER168C.

Structurei

3D structure databases

ProteinModelPortaliP21269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000009678.
HOGENOMiHOG000168085.
InParanoidiP21269.
KOiK00974.
OMAiKETYTDD.
OrthoDBiEOG092C2L2W.

Family and domain databases

CDDicd05398. NT_ClassII-CCAase. 1 hit.
InterProiView protein in InterPro
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
PfamiView protein in Pfam
PF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P21269-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRSTISLLM NSAAQKTMTN SNFVLNAPKI TLTKVEQNIC NLLNDYTDLY
60 70 80 90 100
NQKYHNKPEP LTLRITGGWV RDKLLGQGSH DLDIAINVMS GEQFATGLNE
110 120 130 140 150
YLQQHYAKYG AKPHNIHKID KNPEKSKHLE TATTKLFGVE VDFVNLRSEK
160 170 180 190 200
YTELSRIPKV CFGTPEEDAL RRDATLNALF YNIHKGEVED FTKRGLQDLK
210 220 230 240 250
DGVLRTPLPA KQTFLDDPLR VLRLIRFASR FNFTIDPEVM AEMGDPQINV
260 270 280 290 300
AFNSKISRER VGVEMEKILV GPTPLLALQL IQRAHLENVI FFWHNDSSVV
310 320 330 340 350
KFNEENCQDM DKINHVYNDN ILNSHLKSFI ELYPMFLEKL PILREKIGRS
360 370 380 390 400
PGFQQNFILS AILSPMANLQ IIGNPKKKIN NLVSVTESIV KEGLKLSKND
410 420 430 440 450
AAVIAKTVDS ICSYEEILAK FADRSQLKKS EIGIFLRNFN GEWETAHFAS
460 470 480 490 500
LSDAFLKIPK LETKKIELLF QNYNEFYSYI FDNNLNNCHE LKPIVDGKQM
510 520 530 540
AKLLQMKPGP WLGKINNEAI RWQFDNPTGT DQELITHLKA ILPKYL
Length:546
Mass (Da):62,485
Last modified:May 1, 1991 - v1
Checksum:iC0B2B918596E19D5
GO
Isoform Cytoplasmic+nuclear 1 (identifier: P21269-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Note: Produced by alternative initiation at Met-10 of isoform Mitochondrial.
Show »
Length:537
Mass (Da):61,470
Checksum:iDAB9685770C77A61
GO
Isoform Cytoplasmic+nuclear 2 (identifier: P21269-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: Produced by alternative initiation at Met-18 of isoform Mitochondrial.
Show »
Length:529
Mass (Da):60,638
Checksum:i9479675DBCB98814
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186991 – 17Missing in isoform Cytoplasmic+nuclear 2. CuratedAdd BLAST17
Alternative sequenceiVSP_0186981 – 9Missing in isoform Cytoplasmic+nuclear 1. Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59870 Genomic DNA. Translation: AAA35160.1.
U18922 Genomic DNA. Translation: AAB64695.1.
BK006939 Genomic DNA. Translation: DAA07830.1.
PIRiS11180.
RefSeqiNP_011095.1. NM_001179058.1. [P21269-1]

Genome annotation databases

EnsemblFungiiYER168C; YER168C; YER168C. [P21269-1]
GeneIDi856915.
KEGGisce:YER168C.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiCCA1_YEAST
AccessioniPrimary (citable) accession number: P21269
Secondary accession number(s): D3DM76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 25, 2017
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names