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P21269

- CCA1_YEAST

UniProt

P21269 - CCA1_YEAST

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Protein

CCA tRNA nucleotidyltransferase, mitochondrial

Gene

CCA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site.

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.

GO - Molecular functioni

  1. ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
  4. CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
  5. RNA binding Source: UniProtKB-KW
  6. tRNA adenylyltransferase activity Source: SGD

GO - Biological processi

  1. tRNA 3'-terminal CCA addition Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30329-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA tRNA nucleotidyltransferase, mitochondrial (EC:2.7.7.72)
Alternative name(s):
CCA-adding enzyme
tRNA CCA-pyrophosphorylase
tRNA adenylyltransferase
tRNA nucleotidyltransferase
Gene namesi
Name:CCA1
Synonyms:TNT1
Ordered Locus Names:YER168C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER168c.
SGDiS000000970. CCA1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. mitochondrial matrix Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 546CCA tRNA nucleotidyltransferase, mitochondrialPRO_0000004779
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

MaxQBiP21269.
PaxDbiP21269.
PeptideAtlasiP21269.

Expressioni

Gene expression databases

GenevestigatoriP21269.

Interactioni

Protein-protein interaction databases

BioGridi36921. 18 interactions.
DIPiDIP-2841N.
IntActiP21269. 6 interactions.
MINTiMINT-633371.

Structurei

3D structure databases

ProteinModelPortaliP21269.
SMRiP21269. Positions 26-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0617.
GeneTreeiENSGT00390000009678.
HOGENOMiHOG000168085.
InParanoidiP21269.
KOiK00974.
OMAiISHERIA.
OrthoDBiEOG78SQTC.

Family and domain databases

InterProiIPR002646. PolA_pol_head_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative initiation. Align

Isoform Mitochondrial (identifier: P21269-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRSTISLLM NSAAQKTMTN SNFVLNAPKI TLTKVEQNIC NLLNDYTDLY
60 70 80 90 100
NQKYHNKPEP LTLRITGGWV RDKLLGQGSH DLDIAINVMS GEQFATGLNE
110 120 130 140 150
YLQQHYAKYG AKPHNIHKID KNPEKSKHLE TATTKLFGVE VDFVNLRSEK
160 170 180 190 200
YTELSRIPKV CFGTPEEDAL RRDATLNALF YNIHKGEVED FTKRGLQDLK
210 220 230 240 250
DGVLRTPLPA KQTFLDDPLR VLRLIRFASR FNFTIDPEVM AEMGDPQINV
260 270 280 290 300
AFNSKISRER VGVEMEKILV GPTPLLALQL IQRAHLENVI FFWHNDSSVV
310 320 330 340 350
KFNEENCQDM DKINHVYNDN ILNSHLKSFI ELYPMFLEKL PILREKIGRS
360 370 380 390 400
PGFQQNFILS AILSPMANLQ IIGNPKKKIN NLVSVTESIV KEGLKLSKND
410 420 430 440 450
AAVIAKTVDS ICSYEEILAK FADRSQLKKS EIGIFLRNFN GEWETAHFAS
460 470 480 490 500
LSDAFLKIPK LETKKIELLF QNYNEFYSYI FDNNLNNCHE LKPIVDGKQM
510 520 530 540
AKLLQMKPGP WLGKINNEAI RWQFDNPTGT DQELITHLKA ILPKYL
Length:546
Mass (Da):62,485
Last modified:May 1, 1991 - v1
Checksum:iC0B2B918596E19D5
GO
Isoform Cytoplasmic+nuclear 1 (identifier: P21269-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Note: Produced by alternative initiation at Met-10 of isoform Mitochondrial.

Show »
Length:537
Mass (Da):61,470
Checksum:iDAB9685770C77A61
GO
Isoform Cytoplasmic+nuclear 2 (identifier: P21269-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: Produced by alternative initiation at Met-18 of isoform Mitochondrial.

Show »
Length:529
Mass (Da):60,638
Checksum:i9479675DBCB98814
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1717Missing in isoform Cytoplasmic+nuclear 2. CuratedVSP_018699Add
BLAST
Alternative sequencei1 – 99Missing in isoform Cytoplasmic+nuclear 1. CuratedVSP_018698

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59870 Genomic DNA. Translation: AAA35160.1.
U18922 Genomic DNA. Translation: AAB64695.1.
BK006939 Genomic DNA. Translation: DAA07830.1.
PIRiS11180.
RefSeqiNP_011095.1. NM_001179058.1. [P21269-1]

Genome annotation databases

EnsemblFungiiYER168C; YER168C; YER168C. [P21269-1]
GeneIDi856915.
KEGGisce:YER168C.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59870 Genomic DNA. Translation: AAA35160.1 .
U18922 Genomic DNA. Translation: AAB64695.1 .
BK006939 Genomic DNA. Translation: DAA07830.1 .
PIRi S11180.
RefSeqi NP_011095.1. NM_001179058.1. [P21269-1 ]

3D structure databases

ProteinModelPortali P21269.
SMRi P21269. Positions 26-345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36921. 18 interactions.
DIPi DIP-2841N.
IntActi P21269. 6 interactions.
MINTi MINT-633371.

Proteomic databases

MaxQBi P21269.
PaxDbi P21269.
PeptideAtlasi P21269.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER168C ; YER168C ; YER168C . [P21269-1 ]
GeneIDi 856915.
KEGGi sce:YER168C.

Organism-specific databases

CYGDi YER168c.
SGDi S000000970. CCA1.

Phylogenomic databases

eggNOGi COG0617.
GeneTreei ENSGT00390000009678.
HOGENOMi HOG000168085.
InParanoidi P21269.
KOi K00974.
OMAi ISHERIA.
OrthoDBi EOG78SQTC.

Enzyme and pathway databases

BioCyci YEAST:G3O-30329-MONOMER.

Miscellaneous databases

NextBioi 983365.

Gene expression databases

Genevestigatori P21269.

Family and domain databases

InterProi IPR002646. PolA_pol_head_dom.
[Graphical view ]
Pfami PF01743. PolyA_pol. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae."
    Aebi M., Kirchner G., Chen J.Y., Vijayraghavan U., Jacobson A., Martin N.C., Abelson J.
    J. Biol. Chem. 265:16216-16220(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Interplay of heterogeneous transcriptional start sites and translational selection of AUGs dictate the production of mitochondrial and cytosolic/nuclear tRNA nucleotidyltransferase from the same gene in yeast."
    Wolfe C.L., Lou Y.-C., Hopper A.K., Martin N.C.
    J. Biol. Chem. 269:13361-13366(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE INITIATION, PROTEIN SEQUENCE OF 19-26.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCCA1_YEAST
AccessioniPrimary (citable) accession number: P21269
Secondary accession number(s): D3DM76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 29, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3