Reviewed,
UniProtKB/Swiss-Prot P21269 (CCA1_YEAST)
Last modified
June 16, 2009.
Version 86.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: tRNA nucleotidyltransferase, mitochondrial EC=2.7.7.25 Alternative name(s): tRNA adenylyltransferase tRNA CCA-pyrophosphorylase CCA-adding enzyme | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 546 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site. |
| Catalytic activity | ATP + tRNA(n) = diphosphate + tRNA(n+1). |
| Subcellular location | |
| Miscellaneous | Present with 13500 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Mitochondrion Nucleus |
| Coding sequence diversity | Alternative initiation |
| Domain | Transit peptide |
| Ligand | ATP-binding Nucleotide-binding RNA-binding |
| Molecular function | Nucleotidyltransferase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | tRNA modification Inferred from direct assay. Source: SGD |
| Cellular component | mitochondrial matrix Inferred from direct assay. Source: SGD nucleus Ref.3Non-traceable author statement. Source: SGD |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA bindingInferred from electronic annotation. Source: UniProtKB-KW tRNA adenylyltransferase activityInferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Alternative products
| This entry describes 3 isoforms produced by alternative initiation. [Align] [Select] | ||||||
| Isoform Mitochondrial (identifier: P21269-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Cytoplasmic+nuclear 1 (identifier: P21269-2) The sequence of this isoform differs from the canonical sequence as follows: 1-9: Missing. | ||||||
| Isoform Cytoplasmic+nuclear 2 (identifier: P21269-3) The sequence of this isoform differs from the canonical sequence as follows: 1-17: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Mitochondrion Potential | |||||||
| Chain | ? – 546 | tRNA nucleotidyltransferase, mitochondrial | PRO_0000004779 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 21 | 1 | Phosphoserine Ref.5 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 17 | 17 | Missing in isoform Cytoplasmic+nuclear 2. | VSP_018699 | |||||
| Alternative sequence | 1 – 9 | 9 | Missing in isoform Cytoplasmic+nuclear 1. | VSP_018698 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae." Aebi M., Kirchner G., Chen J.Y., Vijayraghavan U., Jacobson A., Martin N.C., Abelson J. J. Biol. Chem. 265:16216-16220(1990) [PubMed: 2204621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W.Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | "Interplay of heterogeneous transcriptional start sites and translational selection of AUGs dictate the production of mitochondrial and cytosolic/nuclear tRNA nucleotidyltransferase from the same gene in yeast." Wolfe C.L., Lou Y.-C., Hopper A.K., Martin N.C. J. Biol. Chem. 269:13361-13366(1994) [PubMed: 8175766] [Abstract] Cited for: ALTERNATIVE INITIATION, PROTEIN SEQUENCE OF 19-26. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [5] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M59870 Genomic DNA. Translation: AAA35160.1. U18922 Genomic DNA. Translation: AAB64695.1. | |
| PIR | S11180. |
| RefSeq | NP_011095.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:2841N. |
| IntAct | P21269. 7 interactions. |
Proteomic databases | |
| PeptideAtlas | P21269. |
Genome annotation databases | |
| Ensembl | YER168C. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 856915. |
| GenomeReviews | Gene locus YER168C in contig U00092_GR. |
| KEGG | sce:YER168C. |
| NMPDR | fig|4932.3.peg.2181. |
Organism-specific databases | |
| CYGD | YER168c. |
| SGD | S000000970. CCA1. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P21269. |
| OMA | P21269. TCTTKLF. |
Enzyme and pathway databases | |
| BRENDA | 2.7.7.25. 250. |
Gene expression databases | |
| ArrayExpress | P21269. |
| GermOnline | YER168C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR002646. PolyA_pol_reg. [Graphical view] |
| Pfam | PF01743. PolyA_pol. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 983365. |
Entry information
| Entry name | CCA1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P21269 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |

Clusters with


