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P21269

- CCA1_YEAST

UniProt

P21269 - CCA1_YEAST

Protein

CCA tRNA nucleotidyltransferase, mitochondrial

Gene

CCA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site.

    Catalytic activityi

    A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.

    GO - Molecular functioni

    1. ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
    4. CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
    5. RNA binding Source: UniProtKB-KW
    6. tRNA adenylyltransferase activity Source: SGD

    GO - Biological processi

    1. tRNA 3'-terminal CCA addition Source: SGD

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30329-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CCA tRNA nucleotidyltransferase, mitochondrial (EC:2.7.7.72)
    Alternative name(s):
    CCA-adding enzyme
    tRNA CCA-pyrophosphorylase
    tRNA adenylyltransferase
    tRNA nucleotidyltransferase
    Gene namesi
    Name:CCA1
    Synonyms:TNT1
    Ordered Locus Names:YER168C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER168c.
    SGDiS000000970. CCA1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. mitochondrial matrix Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 546CCA tRNA nucleotidyltransferase, mitochondrialPRO_0000004779
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Proteomic databases

    MaxQBiP21269.
    PaxDbiP21269.
    PeptideAtlasiP21269.

    Expressioni

    Gene expression databases

    GenevestigatoriP21269.

    Interactioni

    Protein-protein interaction databases

    BioGridi36921. 17 interactions.
    DIPiDIP-2841N.
    IntActiP21269. 6 interactions.
    MINTiMINT-633371.

    Structurei

    3D structure databases

    ProteinModelPortaliP21269.
    SMRiP21269. Positions 26-345.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0617.
    GeneTreeiENSGT00390000009678.
    HOGENOMiHOG000168085.
    KOiK00974.
    OMAiISHERIA.
    OrthoDBiEOG78SQTC.

    Family and domain databases

    InterProiIPR002646. PolA_pol_head_dom.
    [Graphical view]
    PfamiPF01743. PolyA_pol. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative initiation. Align

    Isoform Mitochondrial (identifier: P21269-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLRSTISLLM NSAAQKTMTN SNFVLNAPKI TLTKVEQNIC NLLNDYTDLY    50
    NQKYHNKPEP LTLRITGGWV RDKLLGQGSH DLDIAINVMS GEQFATGLNE 100
    YLQQHYAKYG AKPHNIHKID KNPEKSKHLE TATTKLFGVE VDFVNLRSEK 150
    YTELSRIPKV CFGTPEEDAL RRDATLNALF YNIHKGEVED FTKRGLQDLK 200
    DGVLRTPLPA KQTFLDDPLR VLRLIRFASR FNFTIDPEVM AEMGDPQINV 250
    AFNSKISRER VGVEMEKILV GPTPLLALQL IQRAHLENVI FFWHNDSSVV 300
    KFNEENCQDM DKINHVYNDN ILNSHLKSFI ELYPMFLEKL PILREKIGRS 350
    PGFQQNFILS AILSPMANLQ IIGNPKKKIN NLVSVTESIV KEGLKLSKND 400
    AAVIAKTVDS ICSYEEILAK FADRSQLKKS EIGIFLRNFN GEWETAHFAS 450
    LSDAFLKIPK LETKKIELLF QNYNEFYSYI FDNNLNNCHE LKPIVDGKQM 500
    AKLLQMKPGP WLGKINNEAI RWQFDNPTGT DQELITHLKA ILPKYL 546
    Length:546
    Mass (Da):62,485
    Last modified:May 1, 1991 - v1
    Checksum:iC0B2B918596E19D5
    GO
    Isoform Cytoplasmic+nuclear 1 (identifier: P21269-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-9: Missing.

    Note: Produced by alternative initiation at Met-10 of isoform Mitochondrial.

    Show »
    Length:537
    Mass (Da):61,470
    Checksum:iDAB9685770C77A61
    GO
    Isoform Cytoplasmic+nuclear 2 (identifier: P21269-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-17: Missing.

    Note: Produced by alternative initiation at Met-18 of isoform Mitochondrial.

    Show »
    Length:529
    Mass (Da):60,638
    Checksum:i9479675DBCB98814
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1717Missing in isoform Cytoplasmic+nuclear 2. CuratedVSP_018699Add
    BLAST
    Alternative sequencei1 – 99Missing in isoform Cytoplasmic+nuclear 1. CuratedVSP_018698

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59870 Genomic DNA. Translation: AAA35160.1.
    U18922 Genomic DNA. Translation: AAB64695.1.
    BK006939 Genomic DNA. Translation: DAA07830.1.
    PIRiS11180.
    RefSeqiNP_011095.1. NM_001179058.1. [P21269-1]

    Genome annotation databases

    EnsemblFungiiYER168C; YER168C; YER168C. [P21269-1]
    GeneIDi856915.
    KEGGisce:YER168C.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59870 Genomic DNA. Translation: AAA35160.1 .
    U18922 Genomic DNA. Translation: AAB64695.1 .
    BK006939 Genomic DNA. Translation: DAA07830.1 .
    PIRi S11180.
    RefSeqi NP_011095.1. NM_001179058.1. [P21269-1 ]

    3D structure databases

    ProteinModelPortali P21269.
    SMRi P21269. Positions 26-345.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36921. 17 interactions.
    DIPi DIP-2841N.
    IntActi P21269. 6 interactions.
    MINTi MINT-633371.

    Proteomic databases

    MaxQBi P21269.
    PaxDbi P21269.
    PeptideAtlasi P21269.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER168C ; YER168C ; YER168C . [P21269-1 ]
    GeneIDi 856915.
    KEGGi sce:YER168C.

    Organism-specific databases

    CYGDi YER168c.
    SGDi S000000970. CCA1.

    Phylogenomic databases

    eggNOGi COG0617.
    GeneTreei ENSGT00390000009678.
    HOGENOMi HOG000168085.
    KOi K00974.
    OMAi ISHERIA.
    OrthoDBi EOG78SQTC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30329-MONOMER.

    Miscellaneous databases

    NextBioi 983365.

    Gene expression databases

    Genevestigatori P21269.

    Family and domain databases

    InterProi IPR002646. PolA_pol_head_dom.
    [Graphical view ]
    Pfami PF01743. PolyA_pol. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae."
      Aebi M., Kirchner G., Chen J.Y., Vijayraghavan U., Jacobson A., Martin N.C., Abelson J.
      J. Biol. Chem. 265:16216-16220(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Interplay of heterogeneous transcriptional start sites and translational selection of AUGs dictate the production of mitochondrial and cytosolic/nuclear tRNA nucleotidyltransferase from the same gene in yeast."
      Wolfe C.L., Lou Y.-C., Hopper A.K., Martin N.C.
      J. Biol. Chem. 269:13361-13366(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE INITIATION, PROTEIN SEQUENCE OF 19-26.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCCA1_YEAST
    AccessioniPrimary (citable) accession number: P21269
    Secondary accession number(s): D3DM76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 13500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3