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P21269 (CCA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CCA tRNA nucleotidyltransferase, mitochondrial

EC=2.7.7.72
Alternative name(s):
CCA-adding enzyme
tRNA CCA-pyrophosphorylase
tRNA adenylyltransferase
tRNA nucleotidyltransferase
Gene names
Name:CCA1
Synonyms:TNT1
Ordered Locus Names:YER168C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length546 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site.

Catalytic activity

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.

Subcellular location

Cytoplasm. Nucleus.

Isoform Mitochondrial: Mitochondrion.

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.

Alternative products

This entry describes 3 isoforms produced by alternative initiation. [Align] [Select]
Isoform Mitochondrial (identifier: P21269-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Cytoplasmic+nuclear 1 (identifier: P21269-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.
Note: Produced by alternative initiation at Met-10 of isoform Mitochondrial.
Isoform Cytoplasmic+nuclear 2 (identifier: P21269-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.
Note: Produced by alternative initiation at Met-18 of isoform Mitochondrial.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 546CCA tRNA nucleotidyltransferase, mitochondrialPRO_0000004779

Natural variations

Alternative sequence1 – 1717Missing in isoform Cytoplasmic+nuclear 2.
VSP_018699
Alternative sequence1 – 99Missing in isoform Cytoplasmic+nuclear 1.
VSP_018698

Sequences

Sequence LengthMass (Da)Tools
Isoform Mitochondrial [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: C0B2B918596E19D5

FASTA54662,485
        10         20         30         40         50         60 
MLRSTISLLM NSAAQKTMTN SNFVLNAPKI TLTKVEQNIC NLLNDYTDLY NQKYHNKPEP 

        70         80         90        100        110        120 
LTLRITGGWV RDKLLGQGSH DLDIAINVMS GEQFATGLNE YLQQHYAKYG AKPHNIHKID 

       130        140        150        160        170        180 
KNPEKSKHLE TATTKLFGVE VDFVNLRSEK YTELSRIPKV CFGTPEEDAL RRDATLNALF 

       190        200        210        220        230        240 
YNIHKGEVED FTKRGLQDLK DGVLRTPLPA KQTFLDDPLR VLRLIRFASR FNFTIDPEVM 

       250        260        270        280        290        300 
AEMGDPQINV AFNSKISRER VGVEMEKILV GPTPLLALQL IQRAHLENVI FFWHNDSSVV 

       310        320        330        340        350        360 
KFNEENCQDM DKINHVYNDN ILNSHLKSFI ELYPMFLEKL PILREKIGRS PGFQQNFILS 

       370        380        390        400        410        420 
AILSPMANLQ IIGNPKKKIN NLVSVTESIV KEGLKLSKND AAVIAKTVDS ICSYEEILAK 

       430        440        450        460        470        480 
FADRSQLKKS EIGIFLRNFN GEWETAHFAS LSDAFLKIPK LETKKIELLF QNYNEFYSYI 

       490        500        510        520        530        540 
FDNNLNNCHE LKPIVDGKQM AKLLQMKPGP WLGKINNEAI RWQFDNPTGT DQELITHLKA 


ILPKYL 

« Hide

Isoform Cytoplasmic+nuclear 1 [UniParc].

Checksum: DAB9685770C77A61
Show »

FASTA53761,470
Isoform Cytoplasmic+nuclear 2 [UniParc].

Checksum: 9479675DBCB98814
Show »

FASTA52960,638

References

« Hide 'large scale' references
[1]"Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae."
Aebi M., Kirchner G., Chen J.Y., Vijayraghavan U., Jacobson A., Martin N.C., Abelson J.
J. Biol. Chem. 265:16216-16220(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Interplay of heterogeneous transcriptional start sites and translational selection of AUGs dictate the production of mitochondrial and cytosolic/nuclear tRNA nucleotidyltransferase from the same gene in yeast."
Wolfe C.L., Lou Y.-C., Hopper A.K., Martin N.C.
J. Biol. Chem. 269:13361-13366(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE INITIATION, PROTEIN SEQUENCE OF 19-26.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59870 Genomic DNA. Translation: AAA35160.1.
U18922 Genomic DNA. Translation: AAB64695.1.
BK006939 Genomic DNA. Translation: DAA07830.1.
PIRS11180.
RefSeqNP_011095.1. NM_001179058.1. [P21269-1]

3D structure databases

ProteinModelPortalP21269.
SMRP21269. Positions 26-345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36921. 17 interactions.
DIPDIP-2841N.
IntActP21269. 6 interactions.
MINTMINT-633371.

Proteomic databases

MaxQBP21269.
PaxDbP21269.
PeptideAtlasP21269.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER168C; YER168C; YER168C. [P21269-1]
GeneID856915.
KEGGsce:YER168C.

Organism-specific databases

CYGDYER168c.
SGDS000000970. CCA1.

Phylogenomic databases

eggNOGCOG0617.
GeneTreeENSGT00390000009678.
HOGENOMHOG000168085.
KOK00974.
OMAISHERIA.
OrthoDBEOG78SQTC.

Enzyme and pathway databases

BioCycYEAST:G3O-30329-MONOMER.

Gene expression databases

GenevestigatorP21269.

Family and domain databases

InterProIPR002646. PolA_pol_head_dom.
[Graphical view]
PfamPF01743. PolyA_pol. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983365.

Entry information

Entry nameCCA1_YEAST
AccessionPrimary (citable) accession number: P21269
Secondary accession number(s): D3DM76
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families