tRNA nucleotidyltransferase, mitochondrial precursor - Saccharomyces cerevisiae (Baker's yeast)

Names and origin

Protein names

Recommended name:
    tRNA nucleotidyltransferase, mitochondrial
    EC=2.7.7.25
Alternative name(s):
    tRNA adenylyltransferase
    tRNA CCA-pyrophosphorylase
    CCA-adding enzyme

Gene names

Name:	CCA1
Synonyms:	TNT1
Ordered Locus Names:	YER168C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site.
Catalytic activity	ATP + tRNA(n) = diphosphate + tRNA(n+1).
Subcellular location	Mitochondrion. Cytoplasm. Nucleus.
Miscellaneous	Present with 13500 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.

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Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion Nucleus
Coding sequence diversity	Alternative initiation
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	tRNA modification Inferred from direct assay. Source: SGD
Cellular component	mitochondrial matrix Inferred from direct assay. Source: SGD nucleus Ref.3 Non-traceable author statement. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA adenylyltransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: P21269-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic+nuclear 1 (identifier: P21269-2) The sequence of this isoform differs from the canonical sequence as follows: 1-9: Missing.

Isoform Cytoplasmic+nuclear 2 (identifier: P21269-3) The sequence of this isoform differs from the canonical sequence as follows: 1-17: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 546

tRNA nucleotidyltransferase, mitochondrial

PRO_0000004779

Amino acid modifications

Modified residue

21

1

Phosphoserine Ref.5

Natural variations

Alternative sequence

1 – 17

17

Missing in isoform Cytoplasmic+nuclear 2.

VSP_018699

Alternative sequence

1 – 9

9

Missing in isoform Cytoplasmic+nuclear 1.

VSP_018698

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Sequences

Sequence		Length	Mass (Da)
Isoform Mitochondrial [UniParc]. Last modified May 1, 1991. Version 1. Checksum: C0B2B918596E19D5 Show »	FASTA	546	62,485
10 20 30 40 50 60 MLRSTISLLM NSAAQKTMTN SNFVLNAPKI TLTKVEQNIC NLLNDYTDLY NQKYHNKPEP 70 80 90 100 110 120 LTLRITGGWV RDKLLGQGSH DLDIAINVMS GEQFATGLNE YLQQHYAKYG AKPHNIHKID 130 140 150 160 170 180 KNPEKSKHLE TATTKLFGVE VDFVNLRSEK YTELSRIPKV CFGTPEEDAL RRDATLNALF 190 200 210 220 230 240 YNIHKGEVED FTKRGLQDLK DGVLRTPLPA KQTFLDDPLR VLRLIRFASR FNFTIDPEVM 250 260 270 280 290 300 AEMGDPQINV AFNSKISRER VGVEMEKILV GPTPLLALQL IQRAHLENVI FFWHNDSSVV 310 320 330 340 350 360 KFNEENCQDM DKINHVYNDN ILNSHLKSFI ELYPMFLEKL PILREKIGRS PGFQQNFILS 370 380 390 400 410 420 AILSPMANLQ IIGNPKKKIN NLVSVTESIV KEGLKLSKND AAVIAKTVDS ICSYEEILAK 430 440 450 460 470 480 FADRSQLKKS EIGIFLRNFN GEWETAHFAS LSDAFLKIPK LETKKIELLF QNYNEFYSYI 490 500 510 520 530 540 FDNNLNNCHE LKPIVDGKQM AKLLQMKPGP WLGKINNEAI RWQFDNPTGT DQELITHLKA ILPKYL « Hide
Isoform Cytoplasmic+nuclear 1. Checksum: DAB9685770C77A61 Show »	FASTA	537	61,470
10 20 30 40 50 60 MNSAAQKTMT NSNFVLNAPK ITLTKVEQNI CNLLNDYTDL YNQKYHNKPE PLTLRITGGW 70 80 90 100 110 120 VRDKLLGQGS HDLDIAINVM SGEQFATGLN EYLQQHYAKY GAKPHNIHKI DKNPEKSKHL 130 140 150 160 170 180 ETATTKLFGV EVDFVNLRSE KYTELSRIPK VCFGTPEEDA LRRDATLNAL FYNIHKGEVE 190 200 210 220 230 240 DFTKRGLQDL KDGVLRTPLP AKQTFLDDPL RVLRLIRFAS RFNFTIDPEV MAEMGDPQIN 250 260 270 280 290 300 VAFNSKISRE RVGVEMEKIL VGPTPLLALQ LIQRAHLENV IFFWHNDSSV VKFNEENCQD 310 320 330 340 350 360 MDKINHVYND NILNSHLKSF IELYPMFLEK LPILREKIGR SPGFQQNFIL SAILSPMANL 370 380 390 400 410 420 QIIGNPKKKI NNLVSVTESI VKEGLKLSKN DAAVIAKTVD SICSYEEILA KFADRSQLKK 430 440 450 460 470 480 SEIGIFLRNF NGEWETAHFA SLSDAFLKIP KLETKKIELL FQNYNEFYSY IFDNNLNNCH 490 500 510 520 530 ELKPIVDGKQ MAKLLQMKPG PWLGKINNEA IRWQFDNPTG TDQELITHLK AILPKYL « Hide
Isoform Cytoplasmic+nuclear 2. Checksum: 9479675DBCB98814 Show »	FASTA	529	60,638
10 20 30 40 50 60 MTNSNFVLNA PKITLTKVEQ NICNLLNDYT DLYNQKYHNK PEPLTLRITG GWVRDKLLGQ 70 80 90 100 110 120 GSHDLDIAIN VMSGEQFATG LNEYLQQHYA KYGAKPHNIH KIDKNPEKSK HLETATTKLF 130 140 150 160 170 180 GVEVDFVNLR SEKYTELSRI PKVCFGTPEE DALRRDATLN ALFYNIHKGE VEDFTKRGLQ 190 200 210 220 230 240 DLKDGVLRTP LPAKQTFLDD PLRVLRLIRF ASRFNFTIDP EVMAEMGDPQ INVAFNSKIS 250 260 270 280 290 300 RERVGVEMEK ILVGPTPLLA LQLIQRAHLE NVIFFWHNDS SVVKFNEENC QDMDKINHVY 310 320 330 340 350 360 NDNILNSHLK SFIELYPMFL EKLPILREKI GRSPGFQQNF ILSAILSPMA NLQIIGNPKK 370 380 390 400 410 420 KINNLVSVTE SIVKEGLKLS KNDAAVIAKT VDSICSYEEI LAKFADRSQL KKSEIGIFLR 430 440 450 460 470 480 NFNGEWETAH FASLSDAFLK IPKLETKKIE LLFQNYNEFY SYIFDNNLNN CHELKPIVDG 490 500 510 520 KQMAKLLQMK PGPWLGKINN EAIRWQFDNP TGTDQELITH LKAILPKYL « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae." Aebi M., Kirchner G., Chen J.Y., Vijayraghavan U., Jacobson A., Martin N.C., Abelson J. J. Biol. Chem. 265:16216-16220(1990) [PubMed: 2204621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Interplay of heterogeneous transcriptional start sites and translational selection of AUGs dictate the production of mitochondrial and cytosolic/nuclear tRNA nucleotidyltransferase from the same gene in yeast." Wolfe C.L., Lou Y.-C., Hopper A.K., Martin N.C. J. Biol. Chem. 269:13361-13366(1994) [PubMed: 8175766] [Abstract] Cited for: ALTERNATIVE INITIATION, PROTEIN SEQUENCE OF 19-26.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	M59870 Genomic DNA. Translation: AAA35160.1. U18922 Genomic DNA. Translation: AAB64695.1.
PIR	S11180.
RefSeq	NP_011095.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2841N.
IntAct	P21269. 7 interactions.
Proteomic databases
PeptideAtlas	P21269.
Genome annotation databases
Ensembl	YER168C. Saccharomyces cerevisiae. [Contig view]
GeneID	856915.
GenomeReviews	Gene locus YER168C in contig U00092_GR.
KEGG	sce:YER168C.
NMPDR	fig\|4932.3.peg.2181.
Organism-specific databases
CYGD	YER168c.
SGD	S000000970. CCA1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P21269.
OMA	P21269. TCTTKLF.
Enzyme and pathway databases
BRENDA	2.7.7.25. 250.
Gene expression databases
ArrayExpress	P21269.
GermOnline	YER168C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002646. PolyA_pol_reg. [Graphical view]
Pfam	PF01743. PolyA_pol. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	983365.

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Entry information

Entry name

CCA1_YEAST

Accession

Primary (citable) accession number: P21269

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents