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P21268

- FAR1_YEAST

UniProt

P21268 - FAR1_YEAST

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Protein
Cyclin-dependent kinase inhibitor FAR1
Gene
FAR1, YJL157C, J0565
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibitor of the cyclin-dependent kinase CDC28. Necessary for cell cycle arrest. Involved in pheromone response. Contributes to mating efficiency. Required for oriented polarization of yeast cells in response to mating pheromones.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri202 – 25251RING-type
Add
BLAST

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell cycle arrest in response to pheromone Source: SGD
  2. cell division Source: UniProtKB-KW
  3. maintenance of protein location in nucleus Source: SGD
  4. negative regulation of protein serine/threonine kinase activity Source: GOC
  5. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Protein kinase inhibitor

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31597-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase inhibitor FAR1
Short name:
CKI FAR1
Alternative name(s):
Factor arrest protein
Gene namesi
Name:FAR1
Ordered Locus Names:YJL157C
ORF Names:J0565
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL157c.
SGDiS000003693. FAR1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. mating projection tip Source: SGD
  3. membrane Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Some mutants appear to be defective in mating because they are unable to locate the mating partner.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi87 – 871S → A: Prevents cell cycle-dependent degradation of FAR1. 1 Publication
Mutagenesisi306 – 3061T → A: Abolishes G1 arrest function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830Cyclin-dependent kinase inhibitor FAR1
PRO_0000055910Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei87 – 871Phosphoserine; by CDC28 Inferred
Modified residuei110 – 1101Phosphoserine1 Publication
Modified residuei114 – 1141Phosphoserine1 Publication
Modified residuei306 – 3061Phosphothreonine1 Publication

Post-translational modificationi

Thought to be phosphorylated by MAP kinase FUS3. Thought to enhance the binding of FAR1 to G1-specific cyclin-dependent kinase (CDK) complexes.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21268.
PaxDbiP21268.
PeptideAtlasiP21268.

Expressioni

Inductioni

By alpha-factor in a cells.

Gene expression databases

GenevestigatoriP21268.

Interactioni

Subunit structurei

Associates with the CDC28-CLN complex.

Protein-protein interaction databases

BioGridi33603. 74 interactions.
DIPiDIP-2229N.
IntActiP21268. 20 interactions.
MINTiMINT-364767.
STRINGi4932.YJL157C.

Structurei

3D structure databases

ProteinModelPortaliP21268.
SMRiP21268. Positions 198-256.

Family & Domainsi

Domaini

There is evidence to suggest that the N-terminal part may be sufficient for cell cycle arrest and the C-terminal may be necessary for some step in mating.

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG331220.
KOiK06652.
OMAiAIFEETH.
OrthoDBiEOG7712HK.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21268-1 [UniParc]FASTAAdd to Basket

« Hide

MKTPTRVSFE KKIHTPPSGD RDAERSPPKK FLRGLSGKVF RKTPEFKKQQ    50
MPTFGYIEES QFTPNLGLMM SKRGNIPKPL NLSKPISPPP SLKKTAGSVA 100
SGFSKTGQLS ALQSPVNITS SNKYNIKATN LTTSLLRESI SDSTTMCDTL 150
SDINLTVMDE DYRIDGDSYY EEDSPTFMIS LERNIKKCNS QFSPKRYIGE 200
KCLICEESIS STFTGEKVVE STCSHTSHYN CYLMLFETLY FQGKFPECKI 250
CGEVSKPKDK DIVPEMVSKL LTGAGAHDDG PSSNMQQQWI DLKTARSFTG 300
EFPQFTPQEQ LIRTADISCD GFRTPRLSNS NQFEAVSYLD SPFLNSPFVN 350
KMATTDPFDL SDDEKLDCDD EIDESAAEVW FSKTGGEHVM VSVKFQEMRT 400
SDDLGVLQDV NHVDHEELEE REKEWKKKID QYIETNVDKD SEFGSLILFD 450
KLMYSDDGEQ WVDNNLVILF SKFLVLFDFE EMKILGKIPR DQFYQVIKFN 500
EDVLLCSLKS TNIPEIYLRF NENCEKWLLP KWKYCLENSS LETLPLSEIV 550
STVKELSHVN IIGALGAPPD VISAQSHDSR LPWKRLHSDT PLKLIVCLNL 600
SHADGELYRK RVLKSVHQIL DGLNTDDLLG IVVVGRDGSG VVGPFGTFIG 650
MINKNWDGWT TFLDNLEVVN PNVFRDEKQQ YKVTLQTCER LASTSAYVDT 700
DDHIATGYAK QILVLNGSDV VDIEHDQKLK KAFDQLSYHW RYEISQRRMT 750
PLNASIKQFL EELHTKRYLD VTLRLPQATF EQVYLGDMAA GEQKTRLIMD 800
EHPHSSLIEI EYFDLVKQQR IHQTLEVPNL 830
Length:830
Mass (Da):94,572
Last modified:November 1, 1995 - v2
Checksum:i834EBB2D7BB9641D
GO

Sequence cautioni

The sequence AAA34600.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti87 – 871S → P in FAR1-22P; induces cell cycle arrest in the absence of alpha-factor.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201D → N1 Publication
Sequence conflicti568 – 5681P → R in AAA34600. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60071 Genomic DNA. Translation: AAA34600.1. Different initiation.
Z49432 Genomic DNA. Translation: CAA89452.1.
BK006943 Genomic DNA. Translation: DAA08646.1.
PIRiS56940.
RefSeqiNP_012378.1. NM_001181590.1.

Genome annotation databases

EnsemblFungiiYJL157C; YJL157C; YJL157C.
GeneIDi853283.
KEGGisce:YJL157C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60071 Genomic DNA. Translation: AAA34600.1 . Different initiation.
Z49432 Genomic DNA. Translation: CAA89452.1 .
BK006943 Genomic DNA. Translation: DAA08646.1 .
PIRi S56940.
RefSeqi NP_012378.1. NM_001181590.1.

3D structure databases

ProteinModelPortali P21268.
SMRi P21268. Positions 198-256.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33603. 74 interactions.
DIPi DIP-2229N.
IntActi P21268. 20 interactions.
MINTi MINT-364767.
STRINGi 4932.YJL157C.

Proteomic databases

MaxQBi P21268.
PaxDbi P21268.
PeptideAtlasi P21268.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL157C ; YJL157C ; YJL157C .
GeneIDi 853283.
KEGGi sce:YJL157C.

Organism-specific databases

CYGDi YJL157c.
SGDi S000003693. FAR1.

Phylogenomic databases

eggNOGi NOG331220.
KOi K06652.
OMAi AIFEETH.
OrthoDBi EOG7712HK.

Enzyme and pathway databases

BioCyci YEAST:G3O-31597-MONOMER.

Miscellaneous databases

NextBioi 973579.

Gene expression databases

Genevestigatori P21268.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2."
    Chang F., Herskowitz I.
    Cell 63:999-1011(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "FAR1 and the G1 phase specificity of cell cycle arrest by mating factor in Saccharomyces cerevisiae."
    McKinney J.D., Cross F.R.
    Mol. Cell. Biol. 15:2509-2516(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO N-TERMINUS, DISRUPTION PHENOTYPE.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: REVIEW.
  6. "Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1."
    Peter M., Herskowitz I.
    Science 265:1228-1231(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "FAR1 is required for oriented polarization of yeast cells in response to mating pheromones."
    Valtz N., Peter M., Herskowitz I.
    J. Cell Biol. 131:863-873(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Functional analysis of FAR1 in yeast."
    Valtz N., Peter M.
    Methods Enzymol. 283:350-365(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. "Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast."
    Henchoz S., Chi Y., Catarin B., Herskowitz I., Deshaies R.J., Peter M.
    Genes Dev. 11:3046-3060(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  10. "Pheromone-dependent G1 cell cycle arrest requires Far1 phosphorylation, but may not involve inhibition of Cdc28-Cln2 kinase, in vivo."
    Gartner A., Jovanovic A., Jeoung D.I., Bourlat S., Cross F.R., Ammerer G.
    Mol. Cell. Biol. 18:3681-3691(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-87 AND THR-306.
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; SER-114 AND THR-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.

Entry informationi

Entry nameiFAR1_YEAST
AccessioniPrimary (citable) accession number: P21268
Secondary accession number(s): D6VW30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi