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P21268 (FAR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase inhibitor FAR1

Short name=CKI FAR1
Alternative name(s):
Factor arrest protein
Gene names
Name:FAR1
Ordered Locus Names:YJL157C
ORF Names:J0565
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of the cyclin-dependent kinase CDC28. Necessary for cell cycle arrest. Involved in pheromone response. Contributes to mating efficiency. Required for oriented polarization of yeast cells in response to mating pheromones.

Subunit structure

Associates with the CDC28-CLN complex.

Induction

By alpha-factor in a cells.

Domain

There is evidence to suggest that the N-terminal part may be sufficient for cell cycle arrest and the C-terminal may be necessary for some step in mating.

Post-translational modification

Thought to be phosphorylated by MAP kinase FUS3. Thought to enhance the binding of FAR1 to G1-specific cyclin-dependent kinase (CDK) complexes.

Disruption phenotype

Some mutants appear to be defective in mating because they are unable to locate the mating partner. Ref.2

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 RING-type zinc finger.

Sequence caution

The sequence AAA34600.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 830830Cyclin-dependent kinase inhibitor FAR1
PRO_0000055910

Regions

Zinc finger202 – 25251RING-type

Amino acid modifications

Modified residue871Phosphoserine; by CDC28 Probable
Modified residue1101Phosphoserine Ref.12
Modified residue1141Phosphoserine Ref.12
Modified residue3061Phosphothreonine Ref.12

Natural variations

Natural variant871S → P in FAR1-22P; induces cell cycle arrest in the absence of alpha-factor.

Experimental info

Mutagenesis871S → A: Prevents cell cycle-dependent degradation of FAR1. Ref.10
Mutagenesis3061T → A: Abolishes G1 arrest function. Ref.10
Sequence conflict201D → N Ref.1
Sequence conflict5681P → R in AAA34600. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P21268 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 834EBB2D7BB9641D

FASTA83094,572
        10         20         30         40         50         60 
MKTPTRVSFE KKIHTPPSGD RDAERSPPKK FLRGLSGKVF RKTPEFKKQQ MPTFGYIEES 

        70         80         90        100        110        120 
QFTPNLGLMM SKRGNIPKPL NLSKPISPPP SLKKTAGSVA SGFSKTGQLS ALQSPVNITS 

       130        140        150        160        170        180 
SNKYNIKATN LTTSLLRESI SDSTTMCDTL SDINLTVMDE DYRIDGDSYY EEDSPTFMIS 

       190        200        210        220        230        240 
LERNIKKCNS QFSPKRYIGE KCLICEESIS STFTGEKVVE STCSHTSHYN CYLMLFETLY 

       250        260        270        280        290        300 
FQGKFPECKI CGEVSKPKDK DIVPEMVSKL LTGAGAHDDG PSSNMQQQWI DLKTARSFTG 

       310        320        330        340        350        360 
EFPQFTPQEQ LIRTADISCD GFRTPRLSNS NQFEAVSYLD SPFLNSPFVN KMATTDPFDL 

       370        380        390        400        410        420 
SDDEKLDCDD EIDESAAEVW FSKTGGEHVM VSVKFQEMRT SDDLGVLQDV NHVDHEELEE 

       430        440        450        460        470        480 
REKEWKKKID QYIETNVDKD SEFGSLILFD KLMYSDDGEQ WVDNNLVILF SKFLVLFDFE 

       490        500        510        520        530        540 
EMKILGKIPR DQFYQVIKFN EDVLLCSLKS TNIPEIYLRF NENCEKWLLP KWKYCLENSS 

       550        560        570        580        590        600 
LETLPLSEIV STVKELSHVN IIGALGAPPD VISAQSHDSR LPWKRLHSDT PLKLIVCLNL 

       610        620        630        640        650        660 
SHADGELYRK RVLKSVHQIL DGLNTDDLLG IVVVGRDGSG VVGPFGTFIG MINKNWDGWT 

       670        680        690        700        710        720 
TFLDNLEVVN PNVFRDEKQQ YKVTLQTCER LASTSAYVDT DDHIATGYAK QILVLNGSDV 

       730        740        750        760        770        780 
VDIEHDQKLK KAFDQLSYHW RYEISQRRMT PLNASIKQFL EELHTKRYLD VTLRLPQATF 

       790        800        810        820        830 
EQVYLGDMAA GEQKTRLIMD EHPHSSLIEI EYFDLVKQQR IHQTLEVPNL 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2."
Chang F., Herskowitz I.
Cell 63:999-1011(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"FAR1 and the G1 phase specificity of cell cycle arrest by mating factor in Saccharomyces cerevisiae."
McKinney J.D., Cross F.R.
Mol. Cell. Biol. 15:2509-2516(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO N-TERMINUS, DISRUPTION PHENOTYPE.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Stop that cell cycle."
Chang F.
Curr. Biol. 3:693-695(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[6]"Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1."
Peter M., Herskowitz I.
Science 265:1228-1231(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"FAR1 is required for oriented polarization of yeast cells in response to mating pheromones."
Valtz N., Peter M., Herskowitz I.
J. Cell Biol. 131:863-873(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Functional analysis of FAR1 in yeast."
Valtz N., Peter M.
Methods Enzymol. 283:350-365(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[9]"Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast."
Henchoz S., Chi Y., Catarin B., Herskowitz I., Deshaies R.J., Peter M.
Genes Dev. 11:3046-3060(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[10]"Pheromone-dependent G1 cell cycle arrest requires Far1 phosphorylation, but may not involve inhibition of Cdc28-Cln2 kinase, in vivo."
Gartner A., Jovanovic A., Jeoung D.I., Bourlat S., Cross F.R., Ammerer G.
Mol. Cell. Biol. 18:3681-3691(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF SER-87 AND THR-306.
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; SER-114 AND THR-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60071 Genomic DNA. Translation: AAA34600.1. Different initiation.
Z49432 Genomic DNA. Translation: CAA89452.1.
BK006943 Genomic DNA. Translation: DAA08646.1.
PIRS56940.
RefSeqNP_012378.1. NM_001181590.1.

3D structure databases

ProteinModelPortalP21268.
SMRP21268. Positions 198-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33603. 73 interactions.
DIPDIP-2229N.
IntActP21268. 20 interactions.
MINTMINT-364767.
STRING4932.YJL157C.

Proteomic databases

PaxDbP21268.
PeptideAtlasP21268.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL157C; YJL157C; YJL157C.
GeneID853283.
KEGGsce:YJL157C.

Organism-specific databases

CYGDYJL157c.
SGDS000003693. FAR1.

Phylogenomic databases

eggNOGNOG331220.
KOK06652.
OMACANTSDE.
OrthoDBEOG7712HK.

Enzyme and pathway databases

BioCycYEAST:G3O-31597-MONOMER.

Gene expression databases

GenevestigatorP21268.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
PROSITEPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973579.

Entry information

Entry nameFAR1_YEAST
AccessionPrimary (citable) accession number: P21268
Secondary accession number(s): D6VW30
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families