Reviewed, UniProtKB/Swiss-Prot P21268 (FAR1_YEAST)

Names and origin

Protein names

Recommended name:
    Cyclin-dependent kinase inhibitor FAR1
      Short name=CKI FAR1
Alternative name(s):
    Factor arrest protein

Gene names

Name:	FAR1
Ordered Locus Names:	YJL157C
ORF Names:	J0565

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibitor of the cyclin-dependent kinase CDC28. Necessary for cell cycle arrest. Involved in pheromone response. Contributes to mating efficiency. Required for oriented polarization of yeast cells in response to mating pheromones.
Subunit structure	Associates with the CDC28-CLN complex.
Induction	By alpha-factor in a cells.
Domain	There is evidence to suggest that the N-terminal part may be sufficient for cell cycle arrest and the C-terminal may be necessary for some step in mating.
Post-translational modification	Thought to be phosphorylated by MAP kinase FUS3. Thought to enhance the binding of FAR1 to G1-specific cyclin-dependent kinase (CDK) complexes. Ref.11 Ref.12 Ref.13
Disruption phenotype	Some mutants appear to be defective in mating because they are unable to locate the mating partner. Ref.2
Miscellaneous	Present with 238 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Protein kinase inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle arrest in response to pheromone Ref.1 Inferred from mutant phenotype. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW maintenance of protein location in nucleus Inferred from mutant phenotype. Source: SGD pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD mating projection tip Inferred from direct assay. Source: SGD membrane Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	cyclin-dependent protein kinase inhibitor activity Ref.5 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BEM1	P29366	2	EBI-6780,EBI-3508

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 830	830	Cyclin-dependent kinase inhibitor FAR1		PRO_0000055910
Regions
	Zinc finger	202 – 252	51	RING-type
Amino acid modifications
	Modified residue	87	1	Phosphoserine; by CDC28 Probable
	Modified residue	98	1	Phosphoserine Ref.13
	Modified residue	110	1	Phosphoserine Ref.12
	Modified residue	114	1	Phosphoserine Ref.11 Ref.12
	Modified residue	306	1	Phosphothreonine Ref.12
Natural variations
	Natural variant	87	1	S → P in FAR1-22P; induces cell cycle arrest in the absence of alpha-factor.
Experimental info
	Mutagenesis	87	1	S → A: Prevents cell cycle-dependent degradation of FAR1. Ref.9
	Mutagenesis	306	1	T → A: Abolishes G1 arrest function. Ref.9
	Sequence conflict	20	1	D → N Ref.1
	Sequence conflict	568	1	P → R in AAA34600. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21268-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 834EBB2D7BB9641D	FASTA	830	94,572
	10 20 30 40 50 60 MKTPTRVSFE KKIHTPPSGD RDAERSPPKK FLRGLSGKVF RKTPEFKKQQ MPTFGYIEES 70 80 90 100 110 120 QFTPNLGLMM SKRGNIPKPL NLSKPISPPP SLKKTAGSVA SGFSKTGQLS ALQSPVNITS 130 140 150 160 170 180 SNKYNIKATN LTTSLLRESI SDSTTMCDTL SDINLTVMDE DYRIDGDSYY EEDSPTFMIS 190 200 210 220 230 240 LERNIKKCNS QFSPKRYIGE KCLICEESIS STFTGEKVVE STCSHTSHYN CYLMLFETLY 250 260 270 280 290 300 FQGKFPECKI CGEVSKPKDK DIVPEMVSKL LTGAGAHDDG PSSNMQQQWI DLKTARSFTG 310 320 330 340 350 360 EFPQFTPQEQ LIRTADISCD GFRTPRLSNS NQFEAVSYLD SPFLNSPFVN KMATTDPFDL 370 380 390 400 410 420 SDDEKLDCDD EIDESAAEVW FSKTGGEHVM VSVKFQEMRT SDDLGVLQDV NHVDHEELEE 430 440 450 460 470 480 REKEWKKKID QYIETNVDKD SEFGSLILFD KLMYSDDGEQ WVDNNLVILF SKFLVLFDFE 490 500 510 520 530 540 EMKILGKIPR DQFYQVIKFN EDVLLCSLKS TNIPEIYLRF NENCEKWLLP KWKYCLENSS 550 560 570 580 590 600 LETLPLSEIV STVKELSHVN IIGALGAPPD VISAQSHDSR LPWKRLHSDT PLKLIVCLNL 610 620 630 640 650 660 SHADGELYRK RVLKSVHQIL DGLNTDDLLG IVVVGRDGSG VVGPFGTFIG MINKNWDGWT 670 680 690 700 710 720 TFLDNLEVVN PNVFRDEKQQ YKVTLQTCER LASTSAYVDT DDHIATGYAK QILVLNGSDV 730 740 750 760 770 780 VDIEHDQKLK KAFDQLSYHW RYEISQRRMT PLNASIKQFL EELHTKRYLD VTLRLPQATF 790 800 810 820 830 EQVYLGDMAA GEQKTRLIMD EHPHSSLIEI EYFDLVKQQR IHQTLEVPNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2." Chang F., Herskowitz I. Cell 63:999-1011(1990) [PubMed: 2147873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"FAR1 and the G1 phase specificity of cell cycle arrest by mating factor in Saccharomyces cerevisiae." McKinney J.D., Cross F.R. Mol. Cell. Biol. 15:2509-2516(1995) [PubMed: 7739534] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS, DISRUPTION PHENOTYPE.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Stop that cell cycle." Chang F. Curr. Biol. 3:693-695(1993) [PubMed: 15335864] [Abstract] Cited for: REVIEW.
[5]	"Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1." Peter M., Herskowitz I. Science 265:1228-1231(1994) [PubMed: 8066461] [Abstract] Cited for: CHARACTERIZATION.
[6]	"FAR1 is required for oriented polarization of yeast cells in response to mating pheromones." Valtz N., Peter M., Herskowitz I. J. Cell Biol. 131:863-873(1995) [PubMed: 7490290] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Functional analysis of FAR1 in yeast." Valtz N., Peter M. Methods Enzymol. 283:350-365(1997) [PubMed: 9251033] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast." Henchoz S., Chi Y., Catarin B., Herskowitz I., Deshaies R.J., Peter M. Genes Dev. 11:3046-3060(1997) [PubMed: 9367986] [Abstract] Cited for: CHARACTERIZATION.
[9]	"Pheromone-dependent G1 cell cycle arrest requires Far1 phosphorylation, but may not involve inhibition of Cdc28-Cln2 kinase, in vivo." Gartner A., Jovanovic A., Jeoung D.I., Bourlat S., Cross F.R., Ammerer G. Mol. Cell. Biol. 18:3681-3691(1998) [PubMed: 9632750] [Abstract] Cited for: MUTAGENESIS OF SER-87 AND THR-306.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, MASS SPECTROMETRY.
[12]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; SER-114 AND THR-306, MASS SPECTROMETRY.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-98, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60071 Genomic DNA. Translation: AAA34600.1. Different initiation. Z49432 Genomic DNA. Translation: CAA89452.1.
PIR	S56940.
RefSeq	NP_012378.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2229N.
IntAct	P21268. 18 interactions.
STRING	P21268.
Proteomic databases
PeptideAtlas	P21268.
PRIDE	P21268.
Genome annotation databases
Ensembl	YJL157C; YJL157C; YJL157C; Saccharomyces cerevisiae. [Genome view]
GeneID	853283.
KEGG	sce:YJL157C.
NMPDR	fig|4932.3.peg.3342.
Organism-specific databases
CYGD	YJL157c.
SGD	S000003693. FAR1.
Phylogenomic databases
OMA	ITSAING
OrthoDB	EOG9CZCZ3
Gene expression databases
ArrayExpress	P21268.
Genevestigator	P21268.
GermOnline	YJL157C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001841. Znf_RING. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973579.

Entry information

Entry name

FAR1_YEAST

Accession

Primary (citable) accession number: P21268

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)