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Protein

Glutathione S-transferase Mu 3

Gene

GSTM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=1.1 mM for 1-chloro-2,4-dinitrobenzene1 Publication
  2. KM=0.084 mM for glutathione1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei120SubstrateBy similarity1

    GO - Molecular functioni

    • enzyme binding Source: BHF-UCL
    • glutathione binding Source: BHF-UCL
    • glutathione transferase activity Source: UniProtKB
    • identical protein binding Source: IntAct
    • protein homodimerization activity Source: MGI

    GO - Biological processi

    • cellular detoxification of nitrogen compound Source: BHF-UCL
    • establishment of blood-nerve barrier Source: ProtInc
    • glutathione derivative biosynthetic process Source: Reactome
    • glutathione metabolic process Source: UniProtKB
    • nitrobenzene metabolic process Source: BHF-UCL
    • response to estrogen Source: UniProtKB
    • xenobiotic catabolic process Source: BHF-UCL

    Keywordsi

    Molecular functionTransferase

    Enzyme and pathway databases

    BRENDAi2.5.1.18 2681
    ReactomeiR-HSA-156590 Glutathione conjugation

    Protein family/group databases

    MoonProtiP21266

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase Mu 3 (EC:2.5.1.18)
    Alternative name(s):
    GST class-mu 3
    GSTM3-3
    Short name:
    hGSTM3-3
    Gene namesi
    Name:GSTM3
    Synonyms:GST5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000134202.10
    HGNCiHGNC:4635 GSTM3
    MIMi138390 gene
    neXtProtiNX_P21266

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi120Y → A: No effect. 1 Publication1
    Mutagenesisi120Y → F: Strongly increased catalytic activity. 1 Publication1
    Mutagenesisi213N → F: Strongly increased catalytic activity. 1 Publication1
    Mutagenesisi213N → G: No effect. 1 Publication1

    Organism-specific databases

    DisGeNETi2947
    OpenTargetsiENSG00000134202
    PharmGKBiPA29024

    Chemistry databases

    ChEMBLiCHEMBL2242
    DrugBankiDB00143 Glutathione
    DB00163 Vitamin E

    Polymorphism and mutation databases

    BioMutaiGSTM3
    DMDMi21264423

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001858221 – 225Glutathione S-transferase Mu 3Add BLAST225

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
    Cross-linki73Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    EPDiP21266
    PaxDbiP21266
    PeptideAtlasiP21266
    PRIDEiP21266

    2D gel databases

    OGPiP21266
    REPRODUCTION-2DPAGEiIPI00246975

    PTM databases

    iPTMnetiP21266
    PhosphoSitePlusiP21266

    Expressioni

    Tissue specificityi

    Testis and brain.

    Gene expression databases

    BgeeiENSG00000134202
    CleanExiHS_GSTM3
    ExpressionAtlasiP21266 baseline and differential
    GenevisibleiP21266 HS

    Organism-specific databases

    HPAiCAB017130
    CAB040583
    HPA035190
    HPA055972

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    • enzyme binding Source: BHF-UCL
    • identical protein binding Source: IntAct
    • protein homodimerization activity Source: MGI

    Protein-protein interaction databases

    BioGridi109202, 26 interactors
    IntActiP21266, 5 interactors
    MINTiP21266
    STRINGi9606.ENSP00000256594

    Structurei

    Secondary structure

    1225
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 15Combined sources9
    Helixi16 – 18Combined sources3
    Helixi19 – 27Combined sources9
    Beta strandi32 – 37Combined sources6
    Beta strandi42 – 44Combined sources3
    Helixi48 – 54Combined sources7
    Beta strandi64 – 69Combined sources6
    Beta strandi72 – 76Combined sources5
    Helixi77 – 87Combined sources11
    Helixi95 – 120Combined sources26
    Helixi124 – 146Combined sources23
    Beta strandi154 – 156Combined sources3
    Helixi159 – 174Combined sources16
    Helixi176 – 179Combined sources4
    Helixi183 – 193Combined sources11
    Helixi196 – 203Combined sources8
    Helixi205 – 208Combined sources4
    Beta strandi211 – 213Combined sources3
    Beta strandi217 – 220Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3GTUX-ray2.80B/D2-225[»]
    ProteinModelPortaliP21266
    SMRiP21266
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21266

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini5 – 92GST N-terminalAdd BLAST88
    Domaini94 – 212GST C-terminalAdd BLAST119

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni11 – 12Glutathione bindingBy similarity2
    Regioni50 – 54Glutathione bindingBy similarity5
    Regioni63 – 64Glutathione bindingBy similarity2
    Regioni76 – 77Glutathione bindingBy similarity2

    Sequence similaritiesi

    Belongs to the GST superfamily. Mu family.Curated

    Phylogenomic databases

    eggNOGiENOG410IN5J Eukaryota
    ENOG4110YU0 LUCA
    GeneTreeiENSGT00550000074559
    HOVERGENiHBG106842
    InParanoidiP21266
    KOiK00799
    OMAiDIMENQV
    OrthoDBiEOG091G0K2E
    PhylomeDBiP21266
    TreeFamiTF353040

    Family and domain databases

    InterProiView protein in InterPro
    IPR010987 Glutathione-S-Trfase_C-like
    IPR036282 Glutathione-S-Trfase_C_sf
    IPR004045 Glutathione_S-Trfase_N
    IPR004046 GST_C
    IPR003081 GST_mu
    IPR036249 Thioredoxin-like_sf
    PfamiView protein in Pfam
    PF00043 GST_C, 1 hit
    PF02798 GST_N, 1 hit
    PRINTSiPR01267 GSTRNSFRASEM
    SUPFAMiSSF47616 SSF47616, 1 hit
    SSF52833 SSF52833, 1 hit
    PROSITEiView protein in PROSITE
    PS50405 GST_CTER, 1 hit
    PS50404 GST_NTER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P21266-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSCESSMVLG YWDIRGLAHA IRLLLEFTDT SYEEKRYTCG EAPDYDRSQW
    60 70 80 90 100
    LDVKFKLDLD FPNLPYLLDG KNKITQSNAI LRYIARKHNM CGETEEEKIR
    110 120 130 140 150
    VDIIENQVMD FRTQLIRLCY SSDHEKLKPQ YLEELPGQLK QFSMFLGKFS
    160 170 180 190 200
    WFAGEKLTFV DFLTYDILDQ NRIFDPKCLD EFPNLKAFMC RFEALEKIAA
    210 220
    YLQSDQFCKM PINNKMAQWG NKPVC
    Length:225
    Mass (Da):26,560
    Last modified:January 23, 2007 - v3
    Checksum:i79093161ECEF5396
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti147G → W in AAA60964 (PubMed:2345169).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_014498224V → I1 PublicationCorresponds to variant dbSNP:rs7483Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J05459 mRNA Translation: AAA60964.1
    AF043105 Genomic DNA Translation: AAC17866.1
    BT019945 mRNA Translation: AAV38748.1
    BC000088 mRNA Translation: AAH00088.1
    BC008790 mRNA Translation: AAH08790.1
    CCDSiCCDS812.1
    PIRiA35295
    RefSeqiNP_000840.2, NM_000849.4
    UniGeneiHs.2006

    Genome annotation databases

    EnsembliENST00000256594; ENSP00000256594; ENSG00000134202
    ENST00000361066; ENSP00000354357; ENSG00000134202
    GeneIDi2947
    KEGGihsa:2947

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiGSTM3_HUMAN
    AccessioniPrimary (citable) accession number: P21266
    Secondary accession number(s): O60550, Q96HA3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 23, 2007
    Last modified: May 23, 2018
    This is version 194 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

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