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Protein

Glutathione S-transferase Mu 3

Gene

GSTM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=1.1 mM for 1-chloro-2,4-dinitrobenzene1 Publication
  2. KM=0.084 mM for glutathione1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei120SubstrateBy similarity1

    GO - Molecular functioni

    • enzyme binding Source: BHF-UCL
    • glutathione binding Source: BHF-UCL
    • glutathione transferase activity Source: UniProtKB
    • identical protein binding Source: IntAct
    • protein homodimerization activity Source: MGI

    GO - Biological processi

    • cellular detoxification of nitrogen compound Source: BHF-UCL
    • establishment of blood-nerve barrier Source: ProtInc
    • glutathione derivative biosynthetic process Source: Reactome
    • glutathione metabolic process Source: UniProtKB
    • nitrobenzene metabolic process Source: BHF-UCL
    • response to estrogen Source: UniProtKB
    • xenobiotic catabolic process Source: BHF-UCL

    Keywordsi

    Molecular functionTransferase

    Enzyme and pathway databases

    BRENDAi2.5.1.18. 2681.
    ReactomeiR-HSA-156590. Glutathione conjugation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase Mu 3 (EC:2.5.1.18)
    Alternative name(s):
    GST class-mu 3
    GSTM3-3
    Short name:
    hGSTM3-3
    Gene namesi
    Name:GSTM3
    Synonyms:GST5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:4635. GSTM3.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: BHF-UCL
    • cytosol Source: Reactome
    • extracellular exosome Source: UniProtKB
    • nucleus Source: UniProtKB
    • sperm fibrous sheath Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi120Y → A: No effect. 1 Publication1
    Mutagenesisi120Y → F: Strongly increased catalytic activity. 1 Publication1
    Mutagenesisi213N → F: Strongly increased catalytic activity. 1 Publication1
    Mutagenesisi213N → G: No effect. 1 Publication1

    Organism-specific databases

    DisGeNETi2947.
    OpenTargetsiENSG00000134202.
    PharmGKBiPA29024.

    Chemistry databases

    ChEMBLiCHEMBL2242.
    DrugBankiDB00143. Glutathione.
    DB00163. Vitamin E.

    Polymorphism and mutation databases

    BioMutaiGSTM3.
    DMDMi21264423.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001858221 – 225Glutathione S-transferase Mu 3Add BLAST225

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki54Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
    Cross-linki73Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    EPDiP21266.
    PaxDbiP21266.
    PeptideAtlasiP21266.
    PRIDEiP21266.

    2D gel databases

    OGPiP21266.
    REPRODUCTION-2DPAGEiIPI00246975.

    PTM databases

    iPTMnetiP21266.
    PhosphoSitePlusiP21266.

    Expressioni

    Tissue specificityi

    Testis and brain.

    Gene expression databases

    BgeeiENSG00000134202.
    CleanExiHS_GSTM3.
    ExpressionAtlasiP21266. baseline and differential.
    GenevisibleiP21266. HS.

    Organism-specific databases

    HPAiCAB017130.
    CAB040583.
    HPA035190.
    HPA055972.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    • enzyme binding Source: BHF-UCL
    • identical protein binding Source: IntAct
    • protein homodimerization activity Source: MGI

    Protein-protein interaction databases

    BioGridi109202. 24 interactors.
    IntActiP21266. 5 interactors.
    STRINGi9606.ENSP00000256594.

    Structurei

    Secondary structure

    1225
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 15Combined sources9
    Helixi16 – 18Combined sources3
    Helixi19 – 27Combined sources9
    Beta strandi32 – 37Combined sources6
    Beta strandi42 – 44Combined sources3
    Helixi48 – 54Combined sources7
    Beta strandi64 – 69Combined sources6
    Beta strandi72 – 76Combined sources5
    Helixi77 – 87Combined sources11
    Helixi95 – 120Combined sources26
    Helixi124 – 146Combined sources23
    Beta strandi154 – 156Combined sources3
    Helixi159 – 174Combined sources16
    Helixi176 – 179Combined sources4
    Helixi183 – 193Combined sources11
    Helixi196 – 203Combined sources8
    Helixi205 – 208Combined sources4
    Beta strandi211 – 213Combined sources3
    Beta strandi217 – 220Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3GTUX-ray2.80B/D2-225[»]
    ProteinModelPortaliP21266.
    SMRiP21266.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21266.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini5 – 92GST N-terminalAdd BLAST88
    Domaini94 – 212GST C-terminalAdd BLAST119

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni11 – 12Glutathione bindingBy similarity2
    Regioni50 – 54Glutathione bindingBy similarity5
    Regioni63 – 64Glutathione bindingBy similarity2
    Regioni76 – 77Glutathione bindingBy similarity2

    Sequence similaritiesi

    Belongs to the GST superfamily. Mu family.Curated

    Phylogenomic databases

    eggNOGiENOG410IN5J. Eukaryota.
    ENOG4110YU0. LUCA.
    GeneTreeiENSGT00550000074559.
    HOVERGENiHBG106842.
    InParanoidiP21266.
    KOiK00799.
    OMAiDIMENQV.
    OrthoDBiEOG091G0K2E.
    PhylomeDBiP21266.
    TreeFamiTF353040.

    Family and domain databases

    InterProiView protein in InterPro
    IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003081. GST_mu.
    IPR012336. Thioredoxin-like_fold.
    PfamiView protein in Pfam
    PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    PRINTSiPR01267. GSTRNSFRASEM.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiView protein in PROSITE
    PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P21266-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSCESSMVLG YWDIRGLAHA IRLLLEFTDT SYEEKRYTCG EAPDYDRSQW
    60 70 80 90 100
    LDVKFKLDLD FPNLPYLLDG KNKITQSNAI LRYIARKHNM CGETEEEKIR
    110 120 130 140 150
    VDIIENQVMD FRTQLIRLCY SSDHEKLKPQ YLEELPGQLK QFSMFLGKFS
    160 170 180 190 200
    WFAGEKLTFV DFLTYDILDQ NRIFDPKCLD EFPNLKAFMC RFEALEKIAA
    210 220
    YLQSDQFCKM PINNKMAQWG NKPVC
    Length:225
    Mass (Da):26,560
    Last modified:January 23, 2007 - v3
    Checksum:i79093161ECEF5396
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti147G → W in AAA60964 (PubMed:2345169).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_014498224V → I1 PublicationCorresponds to variant dbSNP:rs7483Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J05459 mRNA. Translation: AAA60964.1.
    AF043105 Genomic DNA. Translation: AAC17866.1.
    BT019945 mRNA. Translation: AAV38748.1.
    BC000088 mRNA. Translation: AAH00088.1.
    BC008790 mRNA. Translation: AAH08790.1.
    CCDSiCCDS812.1.
    PIRiA35295.
    RefSeqiNP_000840.2. NM_000849.4.
    UniGeneiHs.2006.

    Genome annotation databases

    EnsembliENST00000256594; ENSP00000256594; ENSG00000134202.
    ENST00000361066; ENSP00000354357; ENSG00000134202.
    GeneIDi2947.
    KEGGihsa:2947.

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiGSTM3_HUMAN
    AccessioniPrimary (citable) accession number: P21266
    Secondary accession number(s): O60550, Q96HA3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 23, 2007
    Last modified: August 30, 2017
    This is version 188 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families