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Protein

Phosphoribosylaminoimidazole carboxylase

Gene

ADE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Phosphoribosylaminoimidazole carboxylase (ADE2)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi138 – 193ATPPROSITE-ProRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: SGD
  • adenine biosynthetic process Source: GO_Central
  • purine nucleobase metabolic process Source: SGD
  • purine nucleotide biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YOR128C-MONOMER.
YEAST:YOR128C-MONOMER.
BRENDAi4.1.1.21. 984.
UniPathwayiUPA00074; UER00130.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole carboxylase (EC:4.1.1.21)
Alternative name(s):
AIR carboxylase
Short name:
AIRC
Gene namesi
Name:ADE2
Ordered Locus Names:YOR128C
ORF Names:O3293, YOR3293C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR128C.
SGDiS000005654. ADE2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000750281 – 571Phosphoribosylaminoimidazole carboxylaseAdd BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei37PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21264.
PRIDEiP21264.
TopDownProteomicsiP21264.

PTM databases

iPTMnetiP21264.

Interactioni

Protein-protein interaction databases

BioGridi34523. 42 interactors.
DIPiDIP-1171N.
IntActiP21264. 11 interactors.
MINTiMINT-387849.

Structurei

3D structure databases

ProteinModelPortaliP21264.
SMRiP21264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini110 – 298ATP-graspPROSITE-ProRule annotationAdd BLAST189

Sequence similaritiesi

In the C-terminal section; belongs to the AIR carboxylase family. Class I subfamily.Curated
Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000034027.
InParanoidiP21264.
KOiK11808.
OMAiACETSQY.
OrthoDBiEOG092C1DWF.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.40.50.7700. 1 hit.
HAMAPiMF_01929. PurE_classI. 1 hit.
MF_01928. PurK. 1 hit.
InterProiIPR016301. Ade2_fungi/plant.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR033747. PurE_ClassI.
IPR000031. PurE_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00731. AIRC. 1 hit.
PF02222. ATP-grasp. 1 hit.
[Graphical view]
PIRSFiPIRSF001340. AIR_carboxylase. 1 hit.
SMARTiSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52255. SSF52255. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01162. purE. 1 hit.
TIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSRTVGILG GGQLGRMIVE AANRLNIKTV ILDAENSPAK QISNSNDHVN
60 70 80 90 100
GSFSNPLDIE KLAEKCDVLT IEIEHVDVPT LKNLQVKHPK LKIYPSPETI
110 120 130 140 150
RLIQDKYIQK EHLIKNGIAV TQSVPVEQAS ETSLLNVGRD LGFPFVLKSR
160 170 180 190 200
TLAYDGRGNF VVKNKEMIPE ALEVLKDRPL YAEKWAPFTK ELAVMIVRSV
210 220 230 240 250
NGLVFSYPIV ETIHKDNICD LCYAPARVPD SVQLKAKLLA ENAIKSFPGC
260 270 280 290 300
GIFGVEMFYL ETGELLINEI APRPHNSGHY TIDACVTSQF EAHLRSILDL
310 320 330 340 350
PMPKNFTSFS TITTNAIMLN VLGDKHTKDK ELETCERALA TPGSSVYLYG
360 370 380 390 400
KESRPNRKVG HINIIASSMA ECEQRLNYIT GRTDIPIKIS VAQKLDLEAM
410 420 430 440 450
VKPLVGIIMG SDSDLPVMSA ACAVLKDFGV PFEVTIVSAH RTPHRMSAYA
460 470 480 490 500
ISASKRGIKT IIAGAGGAAH LPGMVAAMTP LPVIGVPVKG SCLDGVDSLH
510 520 530 540 550
SIVQMPRGVP VATVAINNST NAALLAVRLL GAYDSSYTTK MEQFLLKQEE
560 570
EVLVKAQKLE TVGYEAYLEN K
Length:571
Mass (Da):62,339
Last modified:May 1, 1991 - v1
Checksum:i62D2693916F97AA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101R → G in AAA34407 (PubMed:1514324).Curated1
Sequence conflicti186A → G in AAA34407 (PubMed:1514324).Curated1
Sequence conflicti206S → F in AAA34407 (PubMed:1514324).Curated1
Sequence conflicti241 – 253ENAIK…GCGIF → KMQSNFSRLWYI (PubMed:1514324).CuratedAdd BLAST13
Sequence conflicti387I → L in AAA34407 (PubMed:1514324).Curated1
Sequence conflicti407I → V in AAA34407 (PubMed:1514324).Curated1
Sequence conflicti431P → T in AAA34407 (PubMed:1514324).Curated1
Sequence conflicti434V → L in AAA34407 (PubMed:1514324).Curated1
Sequence conflicti502I → T in AAA34407 (PubMed:1514324).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59824 Genomic DNA. Translation: AAA34401.1.
M58324 Genomic DNA. Translation: AAA34407.1.
X90518 Genomic DNA. Translation: CAA62125.1.
X94335 Genomic DNA. Translation: CAA64047.1.
Z75036 Genomic DNA. Translation: CAA99327.1.
BK006948 Genomic DNA. Translation: DAA10902.1.
PIRiJN0098. DEBYP.
RefSeqiNP_014771.3. NM_001183547.3.

Genome annotation databases

EnsemblFungiiYOR128C; YOR128C; YOR128C.
GeneIDi854295.
KEGGisce:YOR128C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59824 Genomic DNA. Translation: AAA34401.1.
M58324 Genomic DNA. Translation: AAA34407.1.
X90518 Genomic DNA. Translation: CAA62125.1.
X94335 Genomic DNA. Translation: CAA64047.1.
Z75036 Genomic DNA. Translation: CAA99327.1.
BK006948 Genomic DNA. Translation: DAA10902.1.
PIRiJN0098. DEBYP.
RefSeqiNP_014771.3. NM_001183547.3.

3D structure databases

ProteinModelPortaliP21264.
SMRiP21264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34523. 42 interactors.
DIPiDIP-1171N.
IntActiP21264. 11 interactors.
MINTiMINT-387849.

PTM databases

iPTMnetiP21264.

Proteomic databases

MaxQBiP21264.
PRIDEiP21264.
TopDownProteomicsiP21264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR128C; YOR128C; YOR128C.
GeneIDi854295.
KEGGisce:YOR128C.

Organism-specific databases

EuPathDBiFungiDB:YOR128C.
SGDiS000005654. ADE2.

Phylogenomic databases

HOGENOMiHOG000034027.
InParanoidiP21264.
KOiK11808.
OMAiACETSQY.
OrthoDBiEOG092C1DWF.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00130.
BioCyciMetaCyc:YOR128C-MONOMER.
YEAST:YOR128C-MONOMER.
BRENDAi4.1.1.21. 984.

Miscellaneous databases

PROiP21264.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.40.50.7700. 1 hit.
HAMAPiMF_01929. PurE_classI. 1 hit.
MF_01928. PurK. 1 hit.
InterProiIPR016301. Ade2_fungi/plant.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR033747. PurE_ClassI.
IPR000031. PurE_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00731. AIRC. 1 hit.
PF02222. ATP-grasp. 1 hit.
[Graphical view]
PIRSFiPIRSF001340. AIR_carboxylase. 1 hit.
SMARTiSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52255. SSF52255. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01162. purE. 1 hit.
TIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR6_YEAST
AccessioniPrimary (citable) accession number: P21264
Secondary accession number(s): D6W2I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5410 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.