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Reviewed, UniProtKB/Swiss-Prot P21264 (PUR6_YEAST)

Last modified February 9, 2010. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phosphoribosylaminoimidazole carboxylase
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC
Gene names
Name: ADE2
Ordered Locus Names: YOR128C
ORF Names: O3293, YOR3293C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.

Miscellaneous

Present with 5410 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

In the C-terminal section; belongs to the AIR carboxylase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571Phosphoribosylaminoimidazole carboxylase
PRO_0000075028

Regions

Domain110 – 298189ATP-grasp
Nucleotide binding138 – 19356ATP By similarity

Experimental info

Sequence conflict1011R → G in AAA34407. Ref.2
Sequence conflict1861A → G in AAA34407. Ref.2
Sequence conflict2061S → F in AAA34407. Ref.2
Sequence conflict241 – 25313ENAIK…GCGIF → KMQSNFSRLWYI Ref.2
Sequence conflict3871I → L in AAA34407. Ref.2
Sequence conflict4071I → V in AAA34407. Ref.2
Sequence conflict4311P → T in AAA34407. Ref.2
Sequence conflict4341V → L in AAA34407. Ref.2
Sequence conflict5021I → T in AAA34407. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P21264-1 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: 62D2693916F97AA0

FASTA57162,339
        10         20         30         40         50         60 
MDSRTVGILG GGQLGRMIVE AANRLNIKTV ILDAENSPAK QISNSNDHVN GSFSNPLDIE 

        70         80         90        100        110        120 
KLAEKCDVLT IEIEHVDVPT LKNLQVKHPK LKIYPSPETI RLIQDKYIQK EHLIKNGIAV 

       130        140        150        160        170        180 
TQSVPVEQAS ETSLLNVGRD LGFPFVLKSR TLAYDGRGNF VVKNKEMIPE ALEVLKDRPL 

       190        200        210        220        230        240 
YAEKWAPFTK ELAVMIVRSV NGLVFSYPIV ETIHKDNICD LCYAPARVPD SVQLKAKLLA 

       250        260        270        280        290        300 
ENAIKSFPGC GIFGVEMFYL ETGELLINEI APRPHNSGHY TIDACVTSQF EAHLRSILDL 

       310        320        330        340        350        360 
PMPKNFTSFS TITTNAIMLN VLGDKHTKDK ELETCERALA TPGSSVYLYG KESRPNRKVG 

       370        380        390        400        410        420 
HINIIASSMA ECEQRLNYIT GRTDIPIKIS VAQKLDLEAM VKPLVGIIMG SDSDLPVMSA 

       430        440        450        460        470        480 
ACAVLKDFGV PFEVTIVSAH RTPHRMSAYA ISASKRGIKT IIAGAGGAAH LPGMVAAMTP 

       490        500        510        520        530        540 
LPVIGVPVKG SCLDGVDSLH SIVQMPRGVP VATVAINNST NAALLAVRLL GAYDSSYTTK 

       550        560        570 
MEQFLLKQEE EVLVKAQKLE TVGYEAYLEN K 

« Hide

References

« Hide 'large scale' references
[1]"The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors."
Stotz A., Linder P.
Gene 95:91-98(1990) [PubMed: 2253890] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular cloning and analysis of autonomous replicating sequence of Candida maltosa."
Sasnauskas K., Jomantiene R., Lebediene E., Lebedys J., Januska A., Janulaitis A.
Yeast 8:253-259(1992) [PubMed: 1514324] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames."
Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W.
Yeast 12:281-288(1996) [PubMed: 8904341] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"DNA sequencing and analysis of 130 kb from yeast chromosome XV."
Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W.
Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59824 Genomic DNA. Translation: AAA34401.1.
M58324 Genomic DNA. Translation: AAA34407.1.
X90518 Genomic DNA. Translation: CAA62125.1.
X94335 Genomic DNA. Translation: CAA64047.1.
Z75036 Genomic DNA. Translation: CAA99327.1.
PIRDEBYP. JN0098.
RefSeqNP_014771.1.

3D structure databases

SMRP21264. Positions 5-377, 403-571.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1171N.
IntActP21264. 27 interactions.
STRINGP21264.

Proteomic databases

PeptideAtlasP21264.
PRIDEP21264.

Genome annotation databases

EnsemblYOR128C; YOR128C; YOR128C; Saccharomyces cerevisiae. [Genome view]
GeneID854295.
KEGGsce:YOR128C.
NMPDRfig|4932.3.peg.5874.

Organism-specific databases

CYGDYOR128c.
SGDS000005654. ADE2.

Phylogenomic databases

eggNOGfuNOG05150.
HOGENOMHBG516369.
OMATSQFEAH.
OrthoDBEOG9908SB.
PhylomeDBP21264.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-487.
BRENDA4.1.1.21. 250.

Gene expression databases

ArrayExpressP21264.
GenevestigatorP21264.
GermOnlineYOR128C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016301. AIR_COase.
IPR005875. AIR_COase_ATPase-su.
IPR000031. AIR_COase_core.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHERPTHR23047:SF1. PurK_ATP. 1 hit.
PfamPF00731. AIRC. 1 hit.
PF02222. ATP-grasp. 1 hit.
[Graphical view]
PIRSFPIRSF001340. AIR_carboxylase. 1 hit.
TIGRFAMsTIGR01162. purE. 1 hit.
TIGR01161. purK. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio976290.

Entry information

Entry namePUR6_YEAST
AccessionPrimary (citable) accession number: P21264
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: February 9, 2010
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents