Reviewed, UniProtKB/Swiss-Prot P21264 (PUR6_YEAST)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	ADE2
Ordered Locus Names:	YOR128C
ORF Names:	O3293, YOR3293C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Miscellaneous	Present with 5410 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	In the C-terminal section; belongs to the AIR carboxylase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro purine base metabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P87282	1	EBI-14252,EBI-36717
EAF5	P39995	1	EBI-14252,EBI-22312
LYS5	P50113	1	EBI-14252,EBI-10282
PGM2	P37012	1	EBI-14252,EBI-13296
SOH1	P38633	1	EBI-14252,EBI-17658
SRP54	P20424	1	EBI-14252,EBI-17997
TAF9	Q05027	1	EBI-14252,EBI-27500

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Phosphoribosylaminoimidazole carboxylase		PRO_0000075028
Regions
	Domain	110 – 298	189	ATP-grasp
	Nucleotide binding	138 – 193	56	ATP By similarity
Experimental info
	Sequence conflict	101	1	R → G in AAA34407. Ref.2
	Sequence conflict	186	1	A → G in AAA34407. Ref.2
	Sequence conflict	206	1	S → F in AAA34407. Ref.2
	Sequence conflict	241 – 253	13	ENAIK…GCGIF → KMQSNFSRLWYI Ref.2
	Sequence conflict	387	1	I → L in AAA34407. Ref.2
	Sequence conflict	407	1	I → V in AAA34407. Ref.2
	Sequence conflict	431	1	P → T in AAA34407. Ref.2
	Sequence conflict	434	1	V → L in AAA34407. Ref.2
	Sequence conflict	502	1	I → T in AAA34407. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P21264-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 62D2693916F97AA0	FASTA	571	62,339
	10 20 30 40 50 60 MDSRTVGILG GGQLGRMIVE AANRLNIKTV ILDAENSPAK QISNSNDHVN GSFSNPLDIE 70 80 90 100 110 120 KLAEKCDVLT IEIEHVDVPT LKNLQVKHPK LKIYPSPETI RLIQDKYIQK EHLIKNGIAV 130 140 150 160 170 180 TQSVPVEQAS ETSLLNVGRD LGFPFVLKSR TLAYDGRGNF VVKNKEMIPE ALEVLKDRPL 190 200 210 220 230 240 YAEKWAPFTK ELAVMIVRSV NGLVFSYPIV ETIHKDNICD LCYAPARVPD SVQLKAKLLA 250 260 270 280 290 300 ENAIKSFPGC GIFGVEMFYL ETGELLINEI APRPHNSGHY TIDACVTSQF EAHLRSILDL 310 320 330 340 350 360 PMPKNFTSFS TITTNAIMLN VLGDKHTKDK ELETCERALA TPGSSVYLYG KESRPNRKVG 370 380 390 400 410 420 HINIIASSMA ECEQRLNYIT GRTDIPIKIS VAQKLDLEAM VKPLVGIIMG SDSDLPVMSA 430 440 450 460 470 480 ACAVLKDFGV PFEVTIVSAH RTPHRMSAYA ISASKRGIKT IIAGAGGAAH LPGMVAAMTP 490 500 510 520 530 540 LPVIGVPVKG SCLDGVDSLH SIVQMPRGVP VATVAINNST NAALLAVRLL GAYDSSYTTK 550 560 570 MEQFLLKQEE EVLVKAQKLE TVGYEAYLEN K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors." Stotz A., Linder P. Gene 95:91-98(1990) [PubMed: 2253890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and analysis of autonomous replicating sequence of Candida maltosa." Sasnauskas K., Jomantiene R., Lebediene E., Lebedys J., Januska A., Janulaitis A. Yeast 8:253-259(1992) [PubMed: 1514324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames." Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W. Yeast 12:281-288(1996) [PubMed: 8904341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59824 Genomic DNA. Translation: AAA34401.1. M58324 Genomic DNA. Translation: AAA34407.1. X90518 Genomic DNA. Translation: CAA62125.1. X94335 Genomic DNA. Translation: CAA64047.1. Z75036 Genomic DNA. Translation: CAA99327.1.
PIR	DEBYP. JN0098.
RefSeq	NP_014771.1.
3D structure databases
SMR	P21264. Positions 5-377, 403-571.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1171N.
IntAct	P21264. 27 interactions.
STRING	P21264.
Proteomic databases
PeptideAtlas	P21264.
PRIDE	P21264.
Genome annotation databases
Ensembl	YOR128C; YOR128C; YOR128C; Saccharomyces cerevisiae. [Genome view]
GeneID	854295.
KEGG	sce:YOR128C.
NMPDR	fig|4932.3.peg.5874.
Organism-specific databases
CYGD	YOR128c.
SGD	S000005654. ADE2.
Phylogenomic databases
eggNOG	fuNOG05150.
HOGENOM	HBG516369.
OMA	TSQFEAH.
OrthoDB	EOG9908SB.
PhylomeDB	P21264.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-487.
BRENDA	4.1.1.21. 250.
Gene expression databases
ArrayExpress	P21264.
Genevestigator	P21264.
GermOnline	YOR128C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016301. AIR_COase. IPR005875. AIR_COase_ATPase-su. IPR000031. AIR_COase_core. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF00731. AIRC. 1 hit. PF02222. ATP-grasp. 1 hit. [Graphical view]
PIRSF	PIRSF001340. AIR_carboxylase. 1 hit.
TIGRFAMs	TIGR01162. purE. 1 hit. TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976290.

Entry information

Entry name

PUR6_YEAST

Accession

Primary (citable) accession number: P21264

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	February 9, 2010
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)