P21264 (PUR6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole carboxylase
EC=4.1.1.21

Alternative name(s):
AIR carboxylase
Short name=AIRC

Gene names

Name:	ADE2
Ordered Locus Names:	YOR128C
ORF Names:	O3293, YOR3293C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Miscellaneous	Present with 5410 molecules/cell in log phase SD medium.
Sequence similarities	In the C-terminal section; belongs to the AIR carboxylase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase metabolic process Inferred from mutant phenotype PubMed 8939809. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	5-(carboxyamino)imidazole ribonucleotide mutase activity Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Inferred from mutant phenotype PubMed 5767024. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Phosphoribosylaminoimidazole carboxylase		PRO_0000075028
Regions
	Domain	110 – 298	189	ATP-grasp
	Nucleotide binding	138 – 193	56	ATP By similarity
Experimental info
	Sequence conflict	101	1	R → G in AAA34407. Ref.2
	Sequence conflict	186	1	A → G in AAA34407. Ref.2
	Sequence conflict	206	1	S → F in AAA34407. Ref.2
	Sequence conflict	241 – 253	13	ENAIK…GCGIF → KMQSNFSRLWYI Ref.2
	Sequence conflict	387	1	I → L in AAA34407. Ref.2
	Sequence conflict	407	1	I → V in AAA34407. Ref.2
	Sequence conflict	431	1	P → T in AAA34407. Ref.2
	Sequence conflict	434	1	V → L in AAA34407. Ref.2
	Sequence conflict	502	1	I → T in AAA34407. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P21264 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 62D2693916F97AA0	FASTA	571	62,339
	10 20 30 40 50 60 MDSRTVGILG GGQLGRMIVE AANRLNIKTV ILDAENSPAK QISNSNDHVN GSFSNPLDIE 70 80 90 100 110 120 KLAEKCDVLT IEIEHVDVPT LKNLQVKHPK LKIYPSPETI RLIQDKYIQK EHLIKNGIAV 130 140 150 160 170 180 TQSVPVEQAS ETSLLNVGRD LGFPFVLKSR TLAYDGRGNF VVKNKEMIPE ALEVLKDRPL 190 200 210 220 230 240 YAEKWAPFTK ELAVMIVRSV NGLVFSYPIV ETIHKDNICD LCYAPARVPD SVQLKAKLLA 250 260 270 280 290 300 ENAIKSFPGC GIFGVEMFYL ETGELLINEI APRPHNSGHY TIDACVTSQF EAHLRSILDL 310 320 330 340 350 360 PMPKNFTSFS TITTNAIMLN VLGDKHTKDK ELETCERALA TPGSSVYLYG KESRPNRKVG 370 380 390 400 410 420 HINIIASSMA ECEQRLNYIT GRTDIPIKIS VAQKLDLEAM VKPLVGIIMG SDSDLPVMSA 430 440 450 460 470 480 ACAVLKDFGV PFEVTIVSAH RTPHRMSAYA ISASKRGIKT IIAGAGGAAH LPGMVAAMTP 490 500 510 520 530 540 LPVIGVPVKG SCLDGVDSLH SIVQMPRGVP VATVAINNST NAALLAVRLL GAYDSSYTTK 550 560 570 MEQFLLKQEE EVLVKAQKLE TVGYEAYLEN K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors." Stotz A., Linder P. Gene 95:91-98(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and analysis of autonomous replicating sequence of Candida maltosa." Sasnauskas K., Jomantiene R., Lebediene E., Lebedys J., Januska A., Janulaitis A. Yeast 8:253-259(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames." Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W. Yeast 12:281-288(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59824 Genomic DNA. Translation: AAA34401.1. M58324 Genomic DNA. Translation: AAA34407.1. X90518 Genomic DNA. Translation: CAA62125.1. X94335 Genomic DNA. Translation: CAA64047.1. Z75036 Genomic DNA. Translation: CAA99327.1. BK006948 Genomic DNA. Translation: DAA10902.1.
PIR	DEBYP. JN0098.
RefSeq	NP_014771.3. NM_001183547.3.
3D structure databases
ProteinModelPortal	P21264.
SMR	P21264. Positions 16-376, 403-571.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1171N.
IntAct	P21264. 9 interactions.
MINT	MINT-387849.
STRING	4932.YOR128C.
Proteomic databases
PaxDb	P21264.
PeptideAtlas	P21264.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR128C; YOR128C; YOR128C.
GeneID	854295.
KEGG	sce:YOR128C. sce:YOR130C.
Organism-specific databases
CYGD	YOR128c.
SGD	S000005654. ADE2.
Phylogenomic databases
eggNOG	COG0026.
HOGENOM	HOG000034027.
KO	K11808. K15114.
OMA	PLYAEKW.
OrthoDB	EOG4TXG17.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-487.
BRENDA	4.1.1.21. 984.
UniPathway	UPA00074; UER00130.
Gene expression databases
Genevestigator	P21264.
GermOnline	YOR128C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.40.50.7700. 1 hit.
InterPro	IPR016301. AIR_COase. IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000031. N5-CAIR_Mutase_PurE_dom. IPR016185. PreATP-grasp_dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF00731. AIRC. 1 hit. PF02222. ATP-grasp. 1 hit. [Graphical view]
PIRSF	PIRSF001340. AIR_carboxylase. 1 hit.
SMART	SM01001. AIRC. 1 hit. [Graphical view]
SUPFAM	SSF52255. AIR_carboxyl. 1 hit. SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR01162. purE. 1 hit. TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976290.

Entry information

Entry name

PUR6_YEAST

Accession

Primary (citable) accession number: P21264
Secondary accession number(s): D6W2I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program