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P21263

- NEST_RAT

UniProt

P21263 - NEST_RAT

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Protein
Nestin
Gene
Nes
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for brain and eye development By similarity. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.2 Publications

GO - Molecular functioni

  1. CCR5 chemokine receptor binding Source: RGD
  2. intermediate filament binding Source: UniProtKB
  3. structural molecule activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. brain development Source: UniProtKB
  2. embryonic camera-type eye development Source: UniProtKB
  3. neuron differentiation Source: RGD
  4. positive regulation of intermediate filament depolymerization Source: UniProtKB
  5. positive regulation of neural precursor cell proliferation Source: UniProtKB
  6. response to drug Source: RGD
  7. response to ionizing radiation Source: RGD
  8. response to nutrient levels Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Nestin
Gene namesi
Name:Nes
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3162. Nes.

Subcellular locationi

GO - Cellular componenti

  1. intermediate filament Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18931893Nestin
PRO_0000063854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei312 – 3121Phosphoserine By similarity
Modified residuei316 – 3161Phosphothreonine1 Publication
Modified residuei356 – 3561Phosphoserine By similarity
Modified residuei359 – 3591Phosphoserine By similarity
Modified residuei389 – 3891Phosphothreonine By similarity
Modified residuei562 – 5621Phosphoserine By similarity
Modified residuei685 – 6851Phosphoserine By similarity
Modified residuei1145 – 11451Phosphoserine By similarity
Modified residuei1216 – 12161Phosphoserine By similarity
Modified residuei1229 – 12291Phosphoserine By similarity
Modified residuei1570 – 15701Phosphoserine By similarity
Modified residuei1686 – 16861Phosphoserine By similarity
Modified residuei1695 – 16951Phosphoserine By similarity
Modified residuei1772 – 17721Phosphoserine By similarity
Modified residuei1774 – 17741Phosphoserine By similarity
Modified residuei1866 – 18661Phosphoserine By similarity
Modified residuei1889 – 18891Phosphoserine By similarity
Modified residuei1890 – 18901Phosphoserine By similarity

Post-translational modificationi

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP21263.
PRIDEiP21263.

PTM databases

PhosphoSiteiP21263.

Expressioni

Tissue specificityi

CNS stem cells.

Developmental stagei

Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

Gene expression databases

GenevestigatoriP21263.

Interactioni

Subunit structurei

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25% By similarity. Interacts with FHOD3.1 Publication

Protein-protein interaction databases

IntActiP21263. 3 interactions.
MINTiMINT-3376397.
STRINGi10116.ENSRNOP00000025314.

Structurei

3D structure databases

ProteinModelPortaliP21263.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 77Head
Regioni8 – 314307Rod
Add
BLAST
Regioni8 – 4336Coil 1A
Add
BLAST
Regioni44 – 5512Linker 1
Add
BLAST
Regioni56 – 15196Coil 1B
Add
BLAST
Regioni152 – 17423Linker 12
Add
BLAST
Regioni175 – 19319Coil 2A
Add
BLAST
Regioni194 – 1963Linker 2
Regioni197 – 314118Coil 2B
Add
BLAST
Regioni315 – 18931579Tail
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000113766.
HOVERGENiHBG006463.
InParanoidiP21263.
KOiK07609.
PhylomeDBiP21263.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 2 hits.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P21263-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDT     50
SWRARADDEL ASLRILVDQR WREKLEAEVQ RDNLAEELES VAGRCQQVRL 100
ARERTVQEAA CSRRALEAEK NARGWLSTQA AELERELEAL RAAHEEERAH 150
LNAQAACAPR RPPAPPHGSP VRAPEVEDLA RRLGEVWRGA VRDYQERVAH 200
MESSLGQARE RLSQAVRGAR ECRLEVQQLQ ADRDSLQERR EALEQRLEGR 250
WQDRLQATDK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV 300
ATYRTLLEAE NSRLQTPGRG SQASLGFLDP KLKPNFLGIP EDQYLGSVLP 350
ALSPTSFPSP LPNTLETPVT AFLKTQEFLQ ARTPTLASTP IPPISEAPCP 400
PNAEVRAQEV PLSLLQTQAP EPLWAEATVP SSSAILPELE EPGGKQQGHF 450
PDDLTSLATT LNPHHPTLEA KDGESSGSRV SSIFQEDEGQ IWELVEKEAD 500
IEVKVENSSA QKTQESGLDT EETQDSQGPL QKETLKALGE EPLMSLKIQN 550
YETAGKENCN SSTEGHLGTL EGPEKEKQIP LKSLEEKNVE SEKTLENGVP 600
VLSELLGKED TRTEDQELMS PKGTLKRFSS LGKESQEVVR PSKEGNLESW 650
TAFKEESQHP LGFPGAEDQM LERLVEKEDQ SFPRSPEEED QEACRPLQKE 700
NQEPLGYEEA EGQILERLIE KESQESLRSP EEEDQEAGRS LQKGNQEPLG 750
YEEAEGQILE RLIEKESQES LRSAEEEDQE ACRSLQKENQ EPLGYEEAED 800
QILERLIEKE SQESLRSPEE EDQEAGRSLQ KENQEPLGYE EAEDQMLERL 850
IEKESQESLK SPEENQRIGK PLERENQKSL RYLEENQETF VPLESRNQRP 900
LRSLEVEEEE QRIVKPLEKV SQDSLGSLAE ENVQPLRYLE EDNCINKSLL 950
EDKTHKSLGS LEDRNGDSII IPQESETQVS LRPPEEEDQR IVNHLEKESQ 1000
EFSRSSEEEE RVMERSLEGE NHESLSSVEK EDQMVESQLE KESQDSGKSL 1050
EDESQETFGP LEKENAESLR SLAGQDQEEQ KLEQETQQTL RAVGNEQMAV 1100
SPPEKVDPEL PKPLGNDQEI ARSLGKENQE SLVSLKEKGI ETVKSLETEI 1150
IEPLETAEED LERRKSIDTQ EPLWSTEVAR ETVEPPEDEP PGSLGSVDEN 1200
RETLTSLEKE SQELSSLGKW NVETRVEDSQ QCLQVEEGLQ EEQHQESLRE 1250
VKQELPSSGN QQRWEDVVEG KAVGQEAPLA TTGVGTEDKA ELHLRGQGGE 1300
EEAAAEGELL QDIVGEAWSL GSSEPKEQRV PAEALDNLEG GALEVPVAQS 1350
MPEVTERDED RAQAGEQDSI EVTLGLEAAR TGLELEQEVV GLEDPRHFAR 1400
EEAIPPSLGE ESVKAKIAQG LEGPGKEPKE AGALDSGILE LPKTSSEALE 1450
CQGHEESESM EGWEEEEASL ETSDHEGSDA PQPRPPETEE DEGAQAALTA 1500
PGPKLLEPCS PIPILTDAHE LQPQAEGIQE AGWQPEAGSE ALERVENEPE 1550
FGLGEIPEGL QDWEEGREES EADDLGETLP DSTPLGLYLR SPASPKWDLA 1600
GEQRLSPQGD AGKEDWGPAV PAAQGLSGPP EEEEEQGHGS DLSSEEFEDL 1650
GTEASLLPGV PKEVADHVGQ VPPVLQPACW DQGGESDGFA DEEESGEEGE 1700
EEDADEEGAE SGAQWWGSGA SGGGCKVQDI AQRGDPVQES VGVSGLWDDG 1750
LRGAAANVPA LEMVSQDSAE PSGSEESESA SLEGEEGQVT DHLDAPQEVT 1800
SMVPGVGDAF DIGGQSPNLD SEQVNGKMEN GLEQAEGQVV LDGDEDQELL 1850
LQGQEVGALK VPLVASPVHL GPSQPLKFTL SGVDGDSWSS GED 1893
Length:1,893
Mass (Da):208,797
Last modified:May 1, 2007 - v2
Checksum:i03AE6B616A1A7623
GO
Isoform 2 (identifier: P21263-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     736-823: Missing.

Show »
Length:1,805
Mass (Da):198,623
Checksum:iDC2A89784654D647
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei736 – 82388Missing in isoform 2.
VSP_024924Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti168 – 1725GSPVR → RIPGP in AAA41685. 1 Publication
Sequence conflicti425 – 4262AE → LK in AAA41685. 1 Publication
Sequence conflicti460 – 4601T → N in AAA41685. 1 Publication
Sequence conflicti477 – 4771G → E in AAA41685. 1 Publication
Sequence conflicti943 – 9431N → D in AAA41685. 1 Publication
Sequence conflicti1011 – 10111R → Q in AAA41685. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34384 mRNA. Translation: AAA41685.1.
AF538924 mRNA. Translation: AAN33053.1.
PIRiA34736.
RefSeqiNP_037119.1. NM_012987.1.
UniGeneiRn.9701.

Genome annotation databases

GeneIDi25491.
KEGGirno:25491.
UCSCiRGD:3162. rat. [P21263-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34384 mRNA. Translation: AAA41685.1 .
AF538924 mRNA. Translation: AAN33053.1 .
PIRi A34736.
RefSeqi NP_037119.1. NM_012987.1.
UniGenei Rn.9701.

3D structure databases

ProteinModelPortali P21263.
ModBasei Search...

Protein-protein interaction databases

IntActi P21263. 3 interactions.
MINTi MINT-3376397.
STRINGi 10116.ENSRNOP00000025314.

PTM databases

PhosphoSitei P21263.

Proteomic databases

PaxDbi P21263.
PRIDEi P21263.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25491.
KEGGi rno:25491.
UCSCi RGD:3162. rat. [P21263-1 ]

Organism-specific databases

CTDi 10763.
RGDi 3162. Nes.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000113766.
HOVERGENi HBG006463.
InParanoidi P21263.
KOi K07609.
PhylomeDBi P21263.

Miscellaneous databases

NextBioi 606861.
PROi P21263.

Gene expression databases

Genevestigatori P21263.

Family and domain databases

InterProi IPR001664. IF.
IPR018039. Intermediate_filament_CS.
[Graphical view ]
PANTHERi PTHR23239. PTHR23239. 1 hit.
Pfami PF00038. Filament. 2 hits.
[Graphical view ]
PROSITEi PS00226. IF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "CNS stem cells express a new class of intermediate filament protein."
    Lendahl U., Zimmerman L.B., McKay R.D.G.
    Cell 60:585-595(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Nestin promotes the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis."
    Chou Y.-H., Khuon S., Herrmann H., Goldman R.D.
    Mol. Biol. Cell 14:1468-1478(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    Tissue: Glial tumor.
  3. "Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase."
    Sahlgren C.M., Mikhailov A., Hellman J., Chou Y.H., Lendahl U., Goldman R.D., Eriksson J.E.
    J. Biol. Chem. 276:16456-16463(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 314-319, PHOSPHORYLATION AT THR-316.
  4. "Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament."
    Kanaya H., Takeya R., Takeuchi K., Watanabe N., Jing N., Sumimoto H.
    Genes Cells 10:665-678(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FHOD3.
  5. "Nestin is essential for mitogen-stimulated proliferation of neural progenitor cells."
    Xue X.J., Yuan X.B.
    Mol. Cell. Neurosci. 45:26-36(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiNEST_RAT
AccessioniPrimary (citable) accession number: P21263
Secondary accession number(s): Q8CJ14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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