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P21263 (NEST_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nestin
Gene names
Name:Nes
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1893 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for brain and eye development By similarity. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. Ref.2 Ref.5

Subunit structure

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25% By similarity. Interacts with FHOD3. Ref.4

Tissue specificity

CNS stem cells.

Developmental stage

Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

Post-translational modification

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Ref.3

Sequence similarities

Belongs to the intermediate filament family.

Ontologies

Keywords
   Biological processNeurogenesis
   Cellular componentIntermediate filament
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   Molecular functionDevelopmental protein
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processbrain development

Inferred from sequence or structural similarity. Source: UniProtKB

embryonic camera-type eye development

Inferred from sequence or structural similarity. Source: UniProtKB

neuron differentiation

Non-traceable author statement Ref.1. Source: RGD

positive regulation of intermediate filament depolymerization

Inferred from direct assay Ref.2. Source: UniProtKB

positive regulation of neural precursor cell proliferation

Inferred from mutant phenotype Ref.5. Source: UniProtKB

response to drug

Inferred from expression pattern PubMed 17510525. Source: RGD

response to ionizing radiation

Inferred from expression pattern PubMed 12445635. Source: RGD

response to nutrient levels

Inferred from expression pattern PubMed 17065391. Source: RGD

   Cellular_componentintermediate filament

Inferred from direct assay PubMed 12832492. Source: RGD

   Molecular_functionCCR5 chemokine receptor binding

Inferred from physical interaction PubMed 19559698. Source: RGD

intermediate filament binding

Inferred from sequence or structural similarity. Source: UniProtKB

structural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P21263-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P21263-2)

The sequence of this isoform differs from the canonical sequence as follows:
     736-823: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18931893Nestin
PRO_0000063854

Regions

Region1 – 77Head
Region8 – 314307Rod
Region8 – 4336Coil 1A
Region44 – 5512Linker 1
Region56 – 15196Coil 1B
Region152 – 17423Linker 12
Region175 – 19319Coil 2A
Region194 – 1963Linker 2
Region197 – 314118Coil 2B
Region315 – 18931579Tail

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue3121Phosphoserine By similarity
Modified residue3161Phosphothreonine Ref.3
Modified residue3561Phosphoserine By similarity
Modified residue3591Phosphoserine By similarity
Modified residue3891Phosphothreonine By similarity
Modified residue5621Phosphoserine By similarity
Modified residue6851Phosphoserine By similarity
Modified residue11451Phosphoserine By similarity
Modified residue12161Phosphoserine By similarity
Modified residue12291Phosphoserine By similarity
Modified residue15701Phosphoserine By similarity
Modified residue16861Phosphoserine By similarity
Modified residue16951Phosphoserine By similarity
Modified residue17721Phosphoserine By similarity
Modified residue17741Phosphoserine By similarity
Modified residue18661Phosphoserine By similarity
Modified residue18891Phosphoserine By similarity
Modified residue18901Phosphoserine By similarity

Natural variations

Alternative sequence736 – 82388Missing in isoform 2.
VSP_024924

Experimental info

Sequence conflict168 – 1725GSPVR → RIPGP in AAA41685. Ref.1
Sequence conflict425 – 4262AE → LK in AAA41685. Ref.1
Sequence conflict4601T → N in AAA41685. Ref.1
Sequence conflict4771G → E in AAA41685. Ref.1
Sequence conflict9431N → D in AAA41685. Ref.1
Sequence conflict10111R → Q in AAA41685. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 03AE6B616A1A7623

FASTA1,893208,797
        10         20         30         40         50         60 
MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDT SWRARADDEL 

        70         80         90        100        110        120 
ASLRILVDQR WREKLEAEVQ RDNLAEELES VAGRCQQVRL ARERTVQEAA CSRRALEAEK 

       130        140        150        160        170        180 
NARGWLSTQA AELERELEAL RAAHEEERAH LNAQAACAPR RPPAPPHGSP VRAPEVEDLA 

       190        200        210        220        230        240 
RRLGEVWRGA VRDYQERVAH MESSLGQARE RLSQAVRGAR ECRLEVQQLQ ADRDSLQERR 

       250        260        270        280        290        300 
EALEQRLEGR WQDRLQATDK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV 

       310        320        330        340        350        360 
ATYRTLLEAE NSRLQTPGRG SQASLGFLDP KLKPNFLGIP EDQYLGSVLP ALSPTSFPSP 

       370        380        390        400        410        420 
LPNTLETPVT AFLKTQEFLQ ARTPTLASTP IPPISEAPCP PNAEVRAQEV PLSLLQTQAP 

       430        440        450        460        470        480 
EPLWAEATVP SSSAILPELE EPGGKQQGHF PDDLTSLATT LNPHHPTLEA KDGESSGSRV 

       490        500        510        520        530        540 
SSIFQEDEGQ IWELVEKEAD IEVKVENSSA QKTQESGLDT EETQDSQGPL QKETLKALGE 

       550        560        570        580        590        600 
EPLMSLKIQN YETAGKENCN SSTEGHLGTL EGPEKEKQIP LKSLEEKNVE SEKTLENGVP 

       610        620        630        640        650        660 
VLSELLGKED TRTEDQELMS PKGTLKRFSS LGKESQEVVR PSKEGNLESW TAFKEESQHP 

       670        680        690        700        710        720 
LGFPGAEDQM LERLVEKEDQ SFPRSPEEED QEACRPLQKE NQEPLGYEEA EGQILERLIE 

       730        740        750        760        770        780 
KESQESLRSP EEEDQEAGRS LQKGNQEPLG YEEAEGQILE RLIEKESQES LRSAEEEDQE 

       790        800        810        820        830        840 
ACRSLQKENQ EPLGYEEAED QILERLIEKE SQESLRSPEE EDQEAGRSLQ KENQEPLGYE 

       850        860        870        880        890        900 
EAEDQMLERL IEKESQESLK SPEENQRIGK PLERENQKSL RYLEENQETF VPLESRNQRP 

       910        920        930        940        950        960 
LRSLEVEEEE QRIVKPLEKV SQDSLGSLAE ENVQPLRYLE EDNCINKSLL EDKTHKSLGS 

       970        980        990       1000       1010       1020 
LEDRNGDSII IPQESETQVS LRPPEEEDQR IVNHLEKESQ EFSRSSEEEE RVMERSLEGE 

      1030       1040       1050       1060       1070       1080 
NHESLSSVEK EDQMVESQLE KESQDSGKSL EDESQETFGP LEKENAESLR SLAGQDQEEQ 

      1090       1100       1110       1120       1130       1140 
KLEQETQQTL RAVGNEQMAV SPPEKVDPEL PKPLGNDQEI ARSLGKENQE SLVSLKEKGI 

      1150       1160       1170       1180       1190       1200 
ETVKSLETEI IEPLETAEED LERRKSIDTQ EPLWSTEVAR ETVEPPEDEP PGSLGSVDEN 

      1210       1220       1230       1240       1250       1260 
RETLTSLEKE SQELSSLGKW NVETRVEDSQ QCLQVEEGLQ EEQHQESLRE VKQELPSSGN 

      1270       1280       1290       1300       1310       1320 
QQRWEDVVEG KAVGQEAPLA TTGVGTEDKA ELHLRGQGGE EEAAAEGELL QDIVGEAWSL 

      1330       1340       1350       1360       1370       1380 
GSSEPKEQRV PAEALDNLEG GALEVPVAQS MPEVTERDED RAQAGEQDSI EVTLGLEAAR 

      1390       1400       1410       1420       1430       1440 
TGLELEQEVV GLEDPRHFAR EEAIPPSLGE ESVKAKIAQG LEGPGKEPKE AGALDSGILE 

      1450       1460       1470       1480       1490       1500 
LPKTSSEALE CQGHEESESM EGWEEEEASL ETSDHEGSDA PQPRPPETEE DEGAQAALTA 

      1510       1520       1530       1540       1550       1560 
PGPKLLEPCS PIPILTDAHE LQPQAEGIQE AGWQPEAGSE ALERVENEPE FGLGEIPEGL 

      1570       1580       1590       1600       1610       1620 
QDWEEGREES EADDLGETLP DSTPLGLYLR SPASPKWDLA GEQRLSPQGD AGKEDWGPAV 

      1630       1640       1650       1660       1670       1680 
PAAQGLSGPP EEEEEQGHGS DLSSEEFEDL GTEASLLPGV PKEVADHVGQ VPPVLQPACW 

      1690       1700       1710       1720       1730       1740 
DQGGESDGFA DEEESGEEGE EEDADEEGAE SGAQWWGSGA SGGGCKVQDI AQRGDPVQES 

      1750       1760       1770       1780       1790       1800 
VGVSGLWDDG LRGAAANVPA LEMVSQDSAE PSGSEESESA SLEGEEGQVT DHLDAPQEVT 

      1810       1820       1830       1840       1850       1860 
SMVPGVGDAF DIGGQSPNLD SEQVNGKMEN GLEQAEGQVV LDGDEDQELL LQGQEVGALK 

      1870       1880       1890 
VPLVASPVHL GPSQPLKFTL SGVDGDSWSS GED 

« Hide

Isoform 2 [UniParc].

Checksum: DC2A89784654D647
Show »

FASTA1,805198,623

References

[1]"CNS stem cells express a new class of intermediate filament protein."
Lendahl U., Zimmerman L.B., McKay R.D.G.
Cell 60:585-595(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Nestin promotes the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis."
Chou Y.-H., Khuon S., Herrmann H., Goldman R.D.
Mol. Biol. Cell 14:1468-1478(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
Tissue: Glial tumor.
[3]"Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase."
Sahlgren C.M., Mikhailov A., Hellman J., Chou Y.H., Lendahl U., Goldman R.D., Eriksson J.E.
J. Biol. Chem. 276:16456-16463(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 314-319, PHOSPHORYLATION AT THR-316.
[4]"Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament."
Kanaya H., Takeya R., Takeuchi K., Watanabe N., Jing N., Sumimoto H.
Genes Cells 10:665-678(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FHOD3.
[5]"Nestin is essential for mitogen-stimulated proliferation of neural progenitor cells."
Xue X.J., Yuan X.B.
Mol. Cell. Neurosci. 45:26-36(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34384 mRNA. Translation: AAA41685.1.
AF538924 mRNA. Translation: AAN33053.1.
PIRA34736.
RefSeqNP_037119.1. NM_012987.1.
UniGeneRn.9701.

3D structure databases

ProteinModelPortalP21263.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP21263. 3 interactions.
MINTMINT-3376397.
STRING10116.ENSRNOP00000025314.

PTM databases

PhosphoSiteP21263.

Proteomic databases

PaxDbP21263.
PRIDEP21263.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25491.
KEGGrno:25491.
UCSCRGD:3162. rat. [P21263-1]

Organism-specific databases

CTD10763.
RGD3162. Nes.

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000113766.
HOVERGENHBG006463.
InParanoidP21263.
KOK07609.
PhylomeDBP21263.

Gene expression databases

GenevestigatorP21263.

Family and domain databases

InterProIPR001664. IF.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERPTHR23239. PTHR23239. 1 hit.
PfamPF00038. Filament. 2 hits.
[Graphical view]
PROSITEPS00226. IF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio606861.
PROP21263.

Entry information

Entry nameNEST_RAT
AccessionPrimary (citable) accession number: P21263
Secondary accession number(s): Q8CJ14
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families