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P21263

- NEST_RAT

UniProt

P21263 - NEST_RAT

Protein

Nestin

Gene

Nes

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Required for brain and eye development By similarity. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.By similarity2 Publications

    GO - Molecular functioni

    1. CCR5 chemokine receptor binding Source: RGD
    2. intermediate filament binding Source: UniProtKB
    3. structural molecule activity Source: InterPro

    GO - Biological processi

    1. brain development Source: UniProtKB
    2. embryonic camera-type eye development Source: UniProtKB
    3. neuron differentiation Source: RGD
    4. positive regulation of intermediate filament depolymerization Source: UniProtKB
    5. positive regulation of neural precursor cell proliferation Source: UniProtKB
    6. response to drug Source: RGD
    7. response to ionizing radiation Source: RGD
    8. response to nutrient levels Source: RGD

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nestin
    Gene namesi
    Name:Nes
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3162. Nes.

    Subcellular locationi

    GO - Cellular componenti

    1. intermediate filament Source: RGD

    Keywords - Cellular componenti

    Intermediate filament

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18931893NestinPRO_0000063854Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei312 – 3121PhosphoserineBy similarity
    Modified residuei316 – 3161Phosphothreonine1 Publication
    Modified residuei356 – 3561PhosphoserineBy similarity
    Modified residuei359 – 3591PhosphoserineBy similarity
    Modified residuei389 – 3891PhosphothreonineBy similarity
    Modified residuei562 – 5621PhosphoserineBy similarity
    Modified residuei685 – 6851PhosphoserineBy similarity
    Modified residuei1145 – 11451PhosphoserineBy similarity
    Modified residuei1216 – 12161PhosphoserineBy similarity
    Modified residuei1229 – 12291PhosphoserineBy similarity
    Modified residuei1570 – 15701PhosphoserineBy similarity
    Modified residuei1686 – 16861PhosphoserineBy similarity
    Modified residuei1695 – 16951PhosphoserineBy similarity
    Modified residuei1772 – 17721PhosphoserineBy similarity
    Modified residuei1774 – 17741PhosphoserineBy similarity
    Modified residuei1866 – 18661PhosphoserineBy similarity
    Modified residuei1889 – 18891PhosphoserineBy similarity
    Modified residuei1890 – 18901PhosphoserineBy similarity

    Post-translational modificationi

    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP21263.
    PRIDEiP21263.

    PTM databases

    PhosphoSiteiP21263.

    Expressioni

    Tissue specificityi

    CNS stem cells.

    Developmental stagei

    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

    Gene expression databases

    GenevestigatoriP21263.

    Interactioni

    Subunit structurei

    Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25% By similarity. Interacts with FHOD3.By similarity1 Publication

    Protein-protein interaction databases

    IntActiP21263. 3 interactions.
    MINTiMINT-3376397.
    STRINGi10116.ENSRNOP00000025314.

    Structurei

    3D structure databases

    ProteinModelPortaliP21263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 77Head
    Regioni8 – 314307RodAdd
    BLAST
    Regioni8 – 4336Coil 1AAdd
    BLAST
    Regioni44 – 5512Linker 1Add
    BLAST
    Regioni56 – 15196Coil 1BAdd
    BLAST
    Regioni152 – 17423Linker 12Add
    BLAST
    Regioni175 – 19319Coil 2AAdd
    BLAST
    Regioni194 – 1963Linker 2
    Regioni197 – 314118Coil 2BAdd
    BLAST
    Regioni315 – 18931579TailAdd
    BLAST

    Sequence similaritiesi

    Belongs to the intermediate filament family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000113766.
    HOVERGENiHBG006463.
    InParanoidiP21263.
    KOiK07609.
    PhylomeDBiP21263.

    Family and domain databases

    InterProiIPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    [Graphical view]
    PANTHERiPTHR23239. PTHR23239. 1 hit.
    PfamiPF00038. Filament. 2 hits.
    [Graphical view]
    PROSITEiPS00226. IF. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P21263-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDT     50
    SWRARADDEL ASLRILVDQR WREKLEAEVQ RDNLAEELES VAGRCQQVRL 100
    ARERTVQEAA CSRRALEAEK NARGWLSTQA AELERELEAL RAAHEEERAH 150
    LNAQAACAPR RPPAPPHGSP VRAPEVEDLA RRLGEVWRGA VRDYQERVAH 200
    MESSLGQARE RLSQAVRGAR ECRLEVQQLQ ADRDSLQERR EALEQRLEGR 250
    WQDRLQATDK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV 300
    ATYRTLLEAE NSRLQTPGRG SQASLGFLDP KLKPNFLGIP EDQYLGSVLP 350
    ALSPTSFPSP LPNTLETPVT AFLKTQEFLQ ARTPTLASTP IPPISEAPCP 400
    PNAEVRAQEV PLSLLQTQAP EPLWAEATVP SSSAILPELE EPGGKQQGHF 450
    PDDLTSLATT LNPHHPTLEA KDGESSGSRV SSIFQEDEGQ IWELVEKEAD 500
    IEVKVENSSA QKTQESGLDT EETQDSQGPL QKETLKALGE EPLMSLKIQN 550
    YETAGKENCN SSTEGHLGTL EGPEKEKQIP LKSLEEKNVE SEKTLENGVP 600
    VLSELLGKED TRTEDQELMS PKGTLKRFSS LGKESQEVVR PSKEGNLESW 650
    TAFKEESQHP LGFPGAEDQM LERLVEKEDQ SFPRSPEEED QEACRPLQKE 700
    NQEPLGYEEA EGQILERLIE KESQESLRSP EEEDQEAGRS LQKGNQEPLG 750
    YEEAEGQILE RLIEKESQES LRSAEEEDQE ACRSLQKENQ EPLGYEEAED 800
    QILERLIEKE SQESLRSPEE EDQEAGRSLQ KENQEPLGYE EAEDQMLERL 850
    IEKESQESLK SPEENQRIGK PLERENQKSL RYLEENQETF VPLESRNQRP 900
    LRSLEVEEEE QRIVKPLEKV SQDSLGSLAE ENVQPLRYLE EDNCINKSLL 950
    EDKTHKSLGS LEDRNGDSII IPQESETQVS LRPPEEEDQR IVNHLEKESQ 1000
    EFSRSSEEEE RVMERSLEGE NHESLSSVEK EDQMVESQLE KESQDSGKSL 1050
    EDESQETFGP LEKENAESLR SLAGQDQEEQ KLEQETQQTL RAVGNEQMAV 1100
    SPPEKVDPEL PKPLGNDQEI ARSLGKENQE SLVSLKEKGI ETVKSLETEI 1150
    IEPLETAEED LERRKSIDTQ EPLWSTEVAR ETVEPPEDEP PGSLGSVDEN 1200
    RETLTSLEKE SQELSSLGKW NVETRVEDSQ QCLQVEEGLQ EEQHQESLRE 1250
    VKQELPSSGN QQRWEDVVEG KAVGQEAPLA TTGVGTEDKA ELHLRGQGGE 1300
    EEAAAEGELL QDIVGEAWSL GSSEPKEQRV PAEALDNLEG GALEVPVAQS 1350
    MPEVTERDED RAQAGEQDSI EVTLGLEAAR TGLELEQEVV GLEDPRHFAR 1400
    EEAIPPSLGE ESVKAKIAQG LEGPGKEPKE AGALDSGILE LPKTSSEALE 1450
    CQGHEESESM EGWEEEEASL ETSDHEGSDA PQPRPPETEE DEGAQAALTA 1500
    PGPKLLEPCS PIPILTDAHE LQPQAEGIQE AGWQPEAGSE ALERVENEPE 1550
    FGLGEIPEGL QDWEEGREES EADDLGETLP DSTPLGLYLR SPASPKWDLA 1600
    GEQRLSPQGD AGKEDWGPAV PAAQGLSGPP EEEEEQGHGS DLSSEEFEDL 1650
    GTEASLLPGV PKEVADHVGQ VPPVLQPACW DQGGESDGFA DEEESGEEGE 1700
    EEDADEEGAE SGAQWWGSGA SGGGCKVQDI AQRGDPVQES VGVSGLWDDG 1750
    LRGAAANVPA LEMVSQDSAE PSGSEESESA SLEGEEGQVT DHLDAPQEVT 1800
    SMVPGVGDAF DIGGQSPNLD SEQVNGKMEN GLEQAEGQVV LDGDEDQELL 1850
    LQGQEVGALK VPLVASPVHL GPSQPLKFTL SGVDGDSWSS GED 1893
    Length:1,893
    Mass (Da):208,797
    Last modified:May 1, 2007 - v2
    Checksum:i03AE6B616A1A7623
    GO
    Isoform 2 (identifier: P21263-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         736-823: Missing.

    Show »
    Length:1,805
    Mass (Da):198,623
    Checksum:iDC2A89784654D647
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti168 – 1725GSPVR → RIPGP in AAA41685. (PubMed:1689217)Curated
    Sequence conflicti425 – 4262AE → LK in AAA41685. (PubMed:1689217)Curated
    Sequence conflicti460 – 4601T → N in AAA41685. (PubMed:1689217)Curated
    Sequence conflicti477 – 4771G → E in AAA41685. (PubMed:1689217)Curated
    Sequence conflicti943 – 9431N → D in AAA41685. (PubMed:1689217)Curated
    Sequence conflicti1011 – 10111R → Q in AAA41685. (PubMed:1689217)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei736 – 82388Missing in isoform 2. 1 PublicationVSP_024924Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34384 mRNA. Translation: AAA41685.1.
    AF538924 mRNA. Translation: AAN33053.1.
    PIRiA34736.
    RefSeqiNP_037119.1. NM_012987.1.
    UniGeneiRn.9701.

    Genome annotation databases

    GeneIDi25491.
    KEGGirno:25491.
    UCSCiRGD:3162. rat. [P21263-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34384 mRNA. Translation: AAA41685.1 .
    AF538924 mRNA. Translation: AAN33053.1 .
    PIRi A34736.
    RefSeqi NP_037119.1. NM_012987.1.
    UniGenei Rn.9701.

    3D structure databases

    ProteinModelPortali P21263.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P21263. 3 interactions.
    MINTi MINT-3376397.
    STRINGi 10116.ENSRNOP00000025314.

    PTM databases

    PhosphoSitei P21263.

    Proteomic databases

    PaxDbi P21263.
    PRIDEi P21263.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25491.
    KEGGi rno:25491.
    UCSCi RGD:3162. rat. [P21263-1 ]

    Organism-specific databases

    CTDi 10763.
    RGDi 3162. Nes.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000113766.
    HOVERGENi HBG006463.
    InParanoidi P21263.
    KOi K07609.
    PhylomeDBi P21263.

    Miscellaneous databases

    NextBioi 606861.
    PROi P21263.

    Gene expression databases

    Genevestigatori P21263.

    Family and domain databases

    InterProi IPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    [Graphical view ]
    PANTHERi PTHR23239. PTHR23239. 1 hit.
    Pfami PF00038. Filament. 2 hits.
    [Graphical view ]
    PROSITEi PS00226. IF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CNS stem cells express a new class of intermediate filament protein."
      Lendahl U., Zimmerman L.B., McKay R.D.G.
      Cell 60:585-595(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. "Nestin promotes the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis."
      Chou Y.-H., Khuon S., Herrmann H., Goldman R.D.
      Mol. Biol. Cell 14:1468-1478(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
      Tissue: Glial tumor.
    3. "Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase."
      Sahlgren C.M., Mikhailov A., Hellman J., Chou Y.H., Lendahl U., Goldman R.D., Eriksson J.E.
      J. Biol. Chem. 276:16456-16463(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 314-319, PHOSPHORYLATION AT THR-316.
    4. "Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament."
      Kanaya H., Takeya R., Takeuchi K., Watanabe N., Jing N., Sumimoto H.
      Genes Cells 10:665-678(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FHOD3.
    5. "Nestin is essential for mitogen-stimulated proliferation of neural progenitor cells."
      Xue X.J., Yuan X.B.
      Mol. Cell. Neurosci. 45:26-36(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiNEST_RAT
    AccessioniPrimary (citable) accession number: P21263
    Secondary accession number(s): Q8CJ14
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3