P21263 (NEST_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 87.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nestin | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 1893 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Required for brain and eye development By similarity. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. Ref.2 Ref.5 |
| Subunit structure | Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25% By similarity. Interacts with FHOD3. Ref.4 |
| Tissue specificity | CNS stem cells. |
| Developmental stage | Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. |
| Post-translational modification | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Ref.3 |
| Sequence similarities | Belongs to the intermediate filament family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P21263-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P21263-2) The sequence of this isoform differs from the canonical sequence as follows: 736-823: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1893 | 1893 | Nestin | PRO_0000063854 | |||||
Regions | |||||||||
| Region | 1 – 7 | 7 | Head | ||||||
| Region | 8 – 314 | 307 | Rod | ||||||
| Region | 8 – 43 | 36 | Coil 1A | ||||||
| Region | 44 – 55 | 12 | Linker 1 | ||||||
| Region | 56 – 151 | 96 | Coil 1B | ||||||
| Region | 152 – 174 | 23 | Linker 12 | ||||||
| Region | 175 – 193 | 19 | Coil 2A | ||||||
| Region | 194 – 196 | 3 | Linker 2 | ||||||
| Region | 197 – 314 | 118 | Coil 2B | ||||||
| Region | 315 – 1893 | 1579 | Tail | ||||||
Amino acid modifications | |||||||||
| Modified residue | 312 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 316 | 1 | Phosphothreonine Ref.3 | ||||||
| Modified residue | 356 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 359 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 389 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 562 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 685 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1145 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1216 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1229 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1570 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1686 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1695 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1772 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1774 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1889 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1890 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 736 – 823 | 88 | Missing in isoform 2. | VSP_024924 | |||||
Experimental info | |||||||||
| Sequence conflict | 168 – 172 | 5 | GSPVR → RIPGP in AAA41685. Ref.1 | ||||||
| Sequence conflict | 425 – 426 | 2 | AE → LK in AAA41685. Ref.1 | ||||||
| Sequence conflict | 460 | 1 | T → N in AAA41685. Ref.1 | ||||||
| Sequence conflict | 477 | 1 | G → E in AAA41685. Ref.1 | ||||||
| Sequence conflict | 943 | 1 | N → D in AAA41685. Ref.1 | ||||||
| Sequence conflict | 1011 | 1 | R → Q in AAA41685. Ref.1 | ||||||
Sequences
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References
| [1] | "CNS stem cells express a new class of intermediate filament protein." Lendahl U., Zimmerman L.B., McKay R.D.G. Cell 60:585-595(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). |
| [2] | "Nestin promotes the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis." Chou Y.-H., Khuon S., Herrmann H., Goldman R.D. Mol. Biol. Cell 14:1468-1478(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION. Tissue: Glial tumor. |
| [3] | "Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase." Sahlgren C.M., Mikhailov A., Hellman J., Chou Y.H., Lendahl U., Goldman R.D., Eriksson J.E. J. Biol. Chem. 276:16456-16463(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 314-319, PHOSPHORYLATION AT THR-316. |
| [4] | "Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament." Kanaya H., Takeya R., Takeuchi K., Watanabe N., Jing N., Sumimoto H. Genes Cells 10:665-678(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FHOD3. |
| [5] | "Nestin is essential for mitogen-stimulated proliferation of neural progenitor cells." Xue X.J., Yuan X.B. Mol. Cell. Neurosci. 45:26-36(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: Sprague-Dawley. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M34384 mRNA. Translation: AAA41685.1. AF538924 mRNA. Translation: AAN33053.1. |
| IPI | IPI00194103. IPI00201261. |
| PIR | A34736. |
| RefSeq | NP_037119.1. NM_012987.1. |
| UniGene | Rn.9701. |
3D structure databases | |
| ProteinModelPortal | P21263. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P21263. 1 interaction. |
| MINT | MINT-3376397. |
| STRING | 10116.ENSRNOP00000025314. |
PTM databases | |
| PhosphoSite | P21263. |
Proteomic databases | |
| PaxDb | P21263. |
| PRIDE | P21263. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 25491. |
| KEGG | rno:25491. |
| UCSC | RGD:3162. rat. |
Organism-specific databases | |
| CTD | 10763. |
| RGD | 3162. Nes. |
Phylogenomic databases | |
| eggNOG | NOG12793. |
| HOGENOM | HOG000113766. |
| HOVERGEN | HBG006463. |
| InParanoid | P21263. |
| KO | K07609. |
| OrthoDB | EOG49GKHK. |
Gene expression databases | |
| ArrayExpress | P21263. |
| Genevestigator | P21263. |
Family and domain databases | |
| InterPro | IPR016044. F. IPR001664. IF. IPR018039. Intermediate_filament_CS. [Graphical view] |
| PANTHER | PTHR23239. PTHR23239. 1 hit. |
| Pfam | PF00038. Filament. 2 hits. [Graphical view] |
| PROSITE | PS00226. IF. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 606861. |
Entry information
| Entry name | NEST_RAT | ||||||||
| Accession | Primary (citable) accession number: P21263 Secondary accession number(s): Q8CJ14 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |