ID   HEM4_BACSU              Reviewed;         262 AA.
AC   P21248;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   27-MAR-2024, entry version 132.
DE   RecName: Full=Uroporphyrinogen-III synthase;
DE            Short=UROS;
DE            EC=4.2.1.75;
DE   AltName: Full=Hydroxymethylbilane hydrolyase [cyclizing];
DE   AltName: Full=Uroporphyrinogen-III cosynthase;
GN   Name=hemD; OrderedLocusNames=BSU28140;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1672867; DOI=10.1128/jb.173.8.2590-2599.1991;
RA   Hansson M., Rutberg L., Schroeder I., Hederstedt L.;
RT   "The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the
RT   biosynthetic pathway from glutamate to uroporphyrinogen III.";
RL   J. Bacteriol. 173:2590-2599(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-130.
RX   PubMed=2110138; DOI=10.1128/jb.172.5.2250-2258.1990;
RA   Petricek M., Rutberg L., Schroeder I., Hederstedt L.;
RT   "Cloning and characterization of the hemA region of the Bacillus subtilis
RT   chromosome.";
RL   J. Bacteriol. 172:2250-2258(1990).
CC   -!- FUNCTION: Catalyzes cyclization of the linear tetrapyrrole,
CC       hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hydroxymethylbilane = H2O + uroporphyrinogen III;
CC         Xref=Rhea:RHEA:18965, ChEBI:CHEBI:15377, ChEBI:CHEBI:57308,
CC         ChEBI:CHEBI:57845; EC=4.2.1.75;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the uroporphyrinogen-III synthase family.
CC       {ECO:0000305}.
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DR   EMBL; M57676; AAA22513.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14774.1; -; Genomic_DNA.
DR   PIR; B42728; B42728.
DR   RefSeq; NP_390692.1; NC_000964.3.
DR   RefSeq; WP_003229629.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; P21248; -.
DR   SMR; P21248; -.
DR   STRING; 224308.BSU28140; -.
DR   PaxDb; 224308-BSU28140; -.
DR   DNASU; 937287; -.
DR   EnsemblBacteria; CAB14774; CAB14774; BSU_28140.
DR   GeneID; 937287; -.
DR   KEGG; bsu:BSU28140; -.
DR   PATRIC; fig|224308.179.peg.3057; -.
DR   eggNOG; COG1587; Bacteria.
DR   InParanoid; P21248; -.
DR   OrthoDB; 9815856at2; -.
DR   PhylomeDB; P21248; -.
DR   BioCyc; BSUB:BSU28140-MONOMER; -.
DR   UniPathway; UPA00251; UER00320.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0004852; F:uroporphyrinogen-III synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006780; P:uroporphyrinogen III biosynthetic process; IEA:InterPro.
DR   CDD; cd06578; HemD; 1.
DR   Gene3D; 3.40.50.10090; -; 2.
DR   InterPro; IPR036108; 4pyrrol_syn_uPrphyn_synt_sf.
DR   InterPro; IPR003754; 4pyrrol_synth_uPrphyn_synth.
DR   InterPro; IPR039793; UROS/Hem4.
DR   PANTHER; PTHR38042; UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR38042:SF1; UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC; 1.
DR   Pfam; PF02602; HEM4; 1.
DR   SUPFAM; SSF69618; HemD-like; 1.
PE   3: Inferred from homology;
KW   Lyase; Porphyrin biosynthesis; Reference proteome.
FT   CHAIN           1..262
FT                   /note="Uroporphyrinogen-III synthase"
FT                   /id="PRO_0000135240"
SQ   SEQUENCE   262 AA;  29114 MW;  DB4B80D93A643F64 CRC64;
     MENDFPLKGK TVLVTRNKAQ AASFQQKVEA LGGKAVLTSL ITFRRALPND VAEQVREDLA
     APGWLVFTSV NGADFFFSYL KENQLILPAH KKIAAVGEKT ARRLKMHNVS VDVMPQEYIA
     EQLADALKQH AEPGETITVM KGNLSRDVIK QELVPLGFEV KEWVLYETIP DEEGIEALKD
     AAGQYSFDYV TFTSSSTVHT FMHVLGEELK KWKANGTACI SIGPLTNDAL LTYGITSHTP
     DTFTIDGMLE LMCSMSREEE RI
//