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P21248 (HEM4_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uroporphyrinogen-III synthase

Short name=UROS
EC=4.2.1.75
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene names
Name:hemD
Ordered Locus Names:BSU28140
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III By similarity.

Catalytic activity

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the uroporphyrinogen-III synthase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionuroporphyrinogen-III synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Uroporphyrinogen-III synthase
PRO_0000135240

Sequences

Sequence LengthMass (Da)Tools
P21248 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: DB4B80D93A643F64

FASTA26229,114
        10         20         30         40         50         60 
MENDFPLKGK TVLVTRNKAQ AASFQQKVEA LGGKAVLTSL ITFRRALPND VAEQVREDLA 

        70         80         90        100        110        120 
APGWLVFTSV NGADFFFSYL KENQLILPAH KKIAAVGEKT ARRLKMHNVS VDVMPQEYIA 

       130        140        150        160        170        180 
EQLADALKQH AEPGETITVM KGNLSRDVIK QELVPLGFEV KEWVLYETIP DEEGIEALKD 

       190        200        210        220        230        240 
AAGQYSFDYV TFTSSSTVHT FMHVLGEELK KWKANGTACI SIGPLTNDAL LTYGITSHTP 

       250        260 
DTFTIDGMLE LMCSMSREEE RI 

« Hide

References

« Hide 'large scale' references
[1]"The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the biosynthetic pathway from glutamate to uroporphyrinogen III."
Hansson M., Rutberg L., Schroeder I., Hederstedt L.
J. Bacteriol. 173:2590-2599(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Cloning and characterization of the hemA region of the Bacillus subtilis chromosome."
Petricek M., Rutberg L., Schroeder I., Hederstedt L.
J. Bacteriol. 172:2250-2258(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-130.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57676 Genomic DNA. Translation: AAA22513.1.
AL009126 Genomic DNA. Translation: CAB14774.1.
PIRB42728.
RefSeqNP_390692.1. NC_000964.3.

3D structure databases

ProteinModelPortalP21248.
SMRP21248. Positions 37-255.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU28140.

Proteomic databases

PaxDbP21248.

Protocols and materials databases

DNASU937287.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14774; CAB14774; BSU28140.
GeneID937287.
KEGGbsu:BSU28140.
PATRIC18977504. VBIBacSub10457_2941.

Organism-specific databases

GenoListBSU28140. [Micado]

Phylogenomic databases

eggNOGCOG1587.
HOGENOMHOG000247992.
KOK01719.
OMACTEITVY.
OrthoDBEOG6JQH1B.
PhylomeDBP21248.

Enzyme and pathway databases

BioCycBSUB:BSU28140-MONOMER.
UniPathwayUPA00251; UER00320.

Family and domain databases

InterProIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMSSF69618. SSF69618. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHEM4_BACSU
AccessionPrimary (citable) accession number: P21248
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: July 1, 1993
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList